VacA Genotyping Directly from Gastric Biopsy Specimens and Estimation of Mixed Helicobacter pylori Infections in Patients with Duodenal Ulcer and Gastritis

References

• The Eurogast Study Group. Epidemiology of, and risk factor for, Helicobacter pylori infection among 3194 asymptomatic subjects in 17 populations. Gut 1993;34: 1672–6.
   Google Scholar
• Blaser MJ. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis 1990;161:626–33.
   PubMed Web of Science ®Google Scholar
• Marshall BJ. Helicobacter pylori. Am J Gastroenterol 1994;89:S116–28.
   PubMed Web of Science ®Google Scholar
• Cover TL, Blaser MJ. Helicobacter pylori: a bacterial cause of gastritis, peptic ulcer disease, and gastric cancer. Am Soc Microbiol News 1995;61:21–6.
   Google Scholar
• Walker MM, Pretolani S, Gasbarrini G. Gastric carcinoma and gastric lymphoma. Curr Opinion Gastroenterol 1996;12 Suppl 1:33–6.
   Google Scholar
• Blaser MJ. Heterogeneity of Helicobacter pylori. Eur J Gastroenterol Hepatol 1997;9 Suppl 1:S3–6.
   PubMedGoogle Scholar
• Taylor DE, Eaton M, Chang N, Salama SM. Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level. J Bacteriol 1992;174:6800–6.
   PubMed Web of Science ®Google Scholar
• Moran AP. Pathogenic properties of Helicobacter pylori. Scand J Gastroenterol 1996;31 Suppl 215:22–31.
   Google Scholar
• Covacci A, Censini S, Bugnoli M, Petracca R, Burroni D, Macchia G, et al. Molecular characterization of the 128-kDa immunodominant antigen of Helicobacter pylori associated with cytotoxicity and duodenal ulcer. Proc Natl Acad Sci USA 1993;90:5791–5.
   PubMed Web of Science ®Google Scholar
• Cover TL. The vacuolating cytotoxin of Helicobacter pylori. Mol Microbiol 1996;20:241–6.
   PubMed Web of Science ®Google Scholar
• Figura N, Tabaqchali S. Bacterial pathogenic factors. Curr Opinion Gastroenterol 1996;12 Suppl 1:11–5.
   Google Scholar
• Megraud F. Pathogenic diversity of Helicobacter pylori. J Gastroenterol 1997;32:278–81.
   PubMedGoogle Scholar
• Xiang Z, Censini S, Bayeli P, Telford JL, Figura N, Rappuoli R, et al. Analysis of expression of CagA and VacA virulence factors in 43 strains of Helicobacter pylori reveals that clinical isolates can be divided into two major types and that CagA is not necessary for expression of the vacuolating cytotoxin. Infect Immun 1995;63:94–8.
   PubMed Web of Science ®Google Scholar
• Atherton JC, Cao P, Peek RM, Tummuru MKR, Blaser M, Cover TL. Mosaicism in vacuolating cytotoxin alleles of Helicobacter pylori. J Biol Chem 1995;270:17771–7.
   PubMed Web of Science ®Google Scholar
• Strobel S, Bereswill S, Steinbruckner B, Ries H, Kist M. Genotyping of the vacA gene: correlation of genotype with duodenal ulceration and identification of untypeable isolates [abstract]. Gut 1997;41 Suppl 1:A112.
   Google Scholar
• Strobel S, Bereswill S, Balig P, Allgaier P, Sonntag HG, Kirst M. Identification and analysis of a new vacA genotype variant of Helicobacter pylori in different patient groups in Germany. J Clin Microbiol 1998;36:1285–9.
   PubMedGoogle Scholar
• Wang JT, Sheu JC, Lin JT, Wang TH, Wu MS. Direct DNA amplification and restriction pattern analysis of Helicobacter pylori in patients with duodenal ulcer and their families. J Infect Dis 1993;168: 1544–8.
   Google Scholar
• Hammar M, Tyszkiewicz T, Wadstrom T, O’Toole P. Rapid detection of Helicobacter pylori in gastric biopsy material by polymerase chain reaction. J Clin Microbiol 1992;30:54–62.
   Google Scholar
• Censini S, Lange C, Xiang Z, Crabtree JE, Ghiara P, Borodovsky M, et al. cag, a pathogenicity island of Helicobacter pylori, encodes type 1-specific and disease-associated virulence factors. Proc Natl Acad Sci USA 1996;93:14648–53.
   PubMed Web of Science ®Google Scholar
• Weel JF, van der Hulst RW, Gerrits Y, Roorda P, Feller M, Dankert J, et al. The interrelationship between cytotoxin associated gene A, vacuolating cytotoxin, and Helicobacter pylori-related diseases. J Infect Dis 1996;173:1171–5.
   PubMed Web of Science ®Google Scholar
• Ito A, Fujioka T, Kodama K, Nishizono A, Nasu M. Virulence associated genes as markers of strain diversity in Helicobacter pylori infections. J Gastroenterol Hepatol 1997;12:666–9.
   Google Scholar
• Takata T, Fujimoto S, Anzani K, Shirotani T, Okada M, Sawae Y, et al. Analysis of the expression of CagA and VacA and vacuolating activity in 167 isolates from patients with either peptic ulcers or non-ulcer dyspepsia. Am J Gastroenterol 1998;93:30–4.
   PubMedGoogle Scholar
• Shimoyama T, Fukuda S, Tanaka M, Mikami T, Saito Y, Munakata A. High prevalence of the cagA-positive Helicobacter pylori strains in Japanese asymptomatic patients and gastric cancer patients. Scand J Gastroenterol 1997;32:465–8.
   PubMed Web of Science ®Google Scholar
• Maeda S, Ogura K, Yoshida H, Kanai F, Ikenoue T, Kato N, et al. Major virulence factors, VacA and CagA, are commonly positive in Helicobacter pylori isolates. Gut 1998;42:338–43.
   PubMed Web of Science ®Google Scholar
• Ito Y, Azuma T, Ito S, Miyaji H, Hirai M, Yamazaki Y, et al. Analysis and typing of the vacA gene from cagA-positive strains of Helicobacter pylori isolated in Japan. J Clin Microbiol 1997;35:1710–4.
   PubMed Web of Science ®Google Scholar
• Pan Z-J, van der Hulst RWM, Feller M, Xiao SD, Tytgat GNJ, Dankert J, et al. Equally high prevalences of infection with cagA-positive Helicobacter pylori in Chinese patients with peptic ulcer disease and those with chronic gastritis-associated dyspepsia. J Clin Microbiol 1997;35: 1344–7.
   PubMedGoogle Scholar
• Miehlke S, Kibler K, Kim JG, Figura N, Small SM, Grahamand DY, et al. Allelic variation in the cagA gene of Helicobacter pylori obtained from Korea compared to the United States. Am J Gastroenterol 1996;91:1322–5.
   PubMed Web of Science ®Google Scholar
• Yamaoka Y, Kodama T, Kashima K, Graham DY, Sepulveda AR. Variants of the 3′ region of the cagA gene in Helicobacter pylori isolates from patients with different H. pylori-associated diseases. J Clin Microbiol 1998;36: 2258–63.
   PubMed Web of Science ®Google Scholar
• van der Ende A, Pan ZJ, Bart A, van der Hulst RWM, Feller M, Xiao SD, et al. cagA-Positive Helicobacter pylori populations in China and The Netherlands are distinct. Infect Immun 1998;66:1822–6.
   PubMed Web of Science ®Google Scholar
• Go MF, Cissell L, Graham DY, Gutierrez O, Kim JG. cagA polymorphism within geographic region and disease. Gut 1998;43 Suppl 2:A42.
   Google Scholar
• Atherton JC, Peek RM Jr, Tham KT, Cover TL, Blaser MJ. Clinical and pathological importance of heterogeneity in vacA, the vacuolating cytotoxin gene of Helicobacter pylori. Gastroenterology 1997;112:92–9.
   PubMed Web of Science ®Google Scholar
• Pan ZJ, Berg DE, van der Hulst RWM, Su WW, Raudonikiene A, Xiao SD, et al. Prevalence of vacuolating cytotoxin production and distribution of distinct vacA alleles in Helicobacter pylori from China. J Infect Dis 1998;178:220–6.
   Google Scholar
• Wang HJ, Kuo CH, Yeh AAM, Chang PCL, Wang WC. Vacuolating toxin production in clinical isolates of Helicobacter pylori with different vacA genotypes. J Infect Dis 1998;178:207–12.
   Google Scholar
• Atherton JC, Karita M, Gonzalez-Valencia G, Morales MR, Ray KC, Peek RM, et al. Diversity in vacA mid-region sequence but not in signal sequence type among Helicobacter pylori strains from Japan, China, Thailand and Peru [abstract]. Gut 1996;39 Suppl 2:A73.
   PubMedGoogle Scholar
• van Doorn LJ, Figueiredo C, Sanna R, Pena S, Midolo P, Ng EK, et al. Expanding allelic diversity of Helicobacter pylori vacA. J Clin Microbiol 1998;36:2597–603.
   PubMedGoogle Scholar
• Ostrowski J, Hennig E, Regula J, Przytulski K, Lazowska I, Butruk E. H. pylori vacA gene differences and cagA gene existence in patients with duodenal ulcer and gastritis [abstract]. Gut 1996;39 Suppl. 3:A94.
   Google Scholar
• Stephens JC, Stewart JA, Folwell AM, Rathbone BJ. Helicobacter pylori cagA status, vacA genotypes and ulcer disease. Eur J Gastroenterol Hepatol 1998;10:381–4.
   PubMedGoogle Scholar
• Ogura K, Kanai F, Maeda S, Yoshida H, Ogura M, Lan KH, et al. High prevalence of cytotoxin positive Helicobacter pylori in patients unrelated to the presence of peptic ulcers in Japan. Gut 1997;41:463–8.
   PubMed Web of Science ®Google Scholar
• van Doorn LJ, Figueiredo C, Sanna R, Plaisier A, Schneeberger P, de Boer W, et al. Clinical relevance of the cagA, vacA, and iceA status of Helicobacter pylori. Gastroenterology 1998;115: 58–66.
   PubMed Web of Science ®Google Scholar
• Regula J, Hennig E, Burzykowski T, Przytulski K, Dziurkowska-Marek A, Marek T, et al. Multivariate analysis of duodenal ulcer risk factors. Gut 1997;41 Suppl 1:A110.
   Google Scholar
• Dubois A, Berg DE, Incecik ET, Fiala N, Heman-Ackah LM, Perez-Perez GI, et al. Transient and persistent experimental infection of nonhuman primates with Helicobacter pylori: implications for human disease. Infect Immun 1996;64: 2885–91.
   PubMed Web of Science ®Google Scholar
• Fantry GT, Zheng QX, Darwin PE, Rosenstein AH, James SP. Mixed infection with cagA-positive and cagA-negative strains of Helicobacter pylori. Helicobacter 1996;1:98–106.
   Google Scholar
• Dore MP, Osato MS, Kwon DH, Graham DY, El-Zaatari FAK. Repetitive sequence-based PCR (REP-PCR) detects simultaneous multiple strain infection in duodenal ulcer patients [abstract]. Gut 1997;41 Suppl 1:A8.
   Google Scholar
• Kitamoto N, Nakamoto H, Katai A, Takahara N, Nakata H, Tamaki H, et al. Heterogeneity of protein profiles of Helicobacter pylori isolates from individual patients. Helicobacter 1998;3:152–62.
   PubMedGoogle Scholar
• Figura N, Vindigni C, Covacci A, Presenti L, Burroni D, Vernillo R, et al. cagA positive and negative Helicobacter pylori strains are simultaneously present in the stomach of most patients with non-ulcer dyspepsia: relevance to histological damage. Gut 1998;42:772–8.
   PubMed Web of Science ®Google Scholar