A rapid and easy method of detecting levels of gene expression in intact tobacco plants using an Agrobacterium-mediated transient assay: Toward development of nutritional diagnosis based on gene expression

References

• Bevan , M. 1984 . Binary Agrobacterium vectors for plant transformation . Nucl. Acids Res. , 12 : 8711 – 8721 .
   PubMed Web of Science ®Google Scholar
• Bradford , M.M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal. Biochem. , 72 : 248 – 254 .
   PubMed Web of Science ®Google Scholar
• Bray , E.A. and Beachy , R.N. 1985 . Regulation by ABA of β-conglycinin expression in cultured developing soybean cotyledons . Plant Physiol. , 79 : 746 – 750 .
   PubMed Web of Science ®Google Scholar
• Bustos , M.M. , Begum , D. , Kalkan , F.A. , Battraw , M.J. and Hall , T.C. 1991 . Positive and negative cis-acting DNA domains are required for spatial and temporal regulation of gene expression by a seed storage protein promoter . EMBO J. , 10 : 1469 – 1479 .
   PubMedGoogle Scholar
• Cheng , C.-L. , Dewedney , J. , Kleinhofs , A. and Goodman , H.M. 1986 . Cloning and nitrate induction of nitrate reductase mRNA . Proc. Natl. Acad. Sci. USA , 83 : 6825 – 6828 .
   PubMedGoogle Scholar
• Crawford , N.M. , Campbell , W.H. and Davis , R.W. 1986 . Nitrate reductase from squash: cDNA cloning and nitrate regulation . Proc. Natl. Acad. Sci. USA , 83 : 8073 – 8076 .
   PubMedGoogle Scholar
• English , J.J. , Davenport , G.F. , Elmayan , T. , Vaucheret , H. and Baulcomb , D.C. 1997 . Requirement of sense transcription for homology-dependent virus resistance and trans-inactivation . Plant J. , 12 : 597 – 603 .
   Web of Science ®Google Scholar
• Escudero , J. and Hohn , B. 1997 . Transfer and integration of T-DNA without cell injury in the host plant . Plant Cell , 9 : 2135 – 2142 .
   PubMedGoogle Scholar
• Figurski , D.H. and Helinski , D.R. 1979 . Replication of an origin-containing derivative of plasmid RK2 dependent on a plasmid function provided in trans . Proc. Natl. Acad. Sci. USA , 76 : 1648 – 1652 .
   PubMed Web of Science ®Google Scholar
• Frisch , D.A. , Van der Geest , H.M.A. , Dias , K. and Hall , T.C. 1995 . Chromosomal integration is required for spatial regulation of expression from the β-phaseolin promoter . Plant J. , 7 : 503 – 512 .
   Google Scholar
• Fujiwara , T. and Beachy , R.N. 1994 . Tissue-specific and temporal regulation of a β-conglycinin Gene: Roles of the RY repeat and other cis-acting elements . Plant Mol. Biol. , 24 : 261 – 272 .
   PubMed Web of Science ®Google Scholar
• Fujiwara , T. , Naito , S. , Chino , M. and Nagata , T. 1991 . Electroporated protoplasts express seed specific gene promoters . Plant Cell Rep. , 9 : 602 – 606 .
   PubMedGoogle Scholar
• Galangau , F. , Daniel-Vedele , F. , Moureaux , T. , Dorbe , M.F. , Leydecker , M.T. and Caboche , M. 1988 . Expression of leaf nitrate reductase genes from tomato and tobacco in relation to light-dark regimes and nitrate supply . Plant Physiol. , 88 : 383 – 388 .
   PubMed Web of Science ®Google Scholar
• Hiei , Y. , Ohta , S. , Komari , T. and Kumashiro , T. 1994 . Efficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA . Plant J. , 6 : 271 – 282 .
   PubMed Web of Science ®Google Scholar
• Hirai , M.Y. , Fujiwara , T. , Chino , M. and Naito , S. 1995 . Effects of sulfate concentration on the expression of a soybean seed storage protein gene and its reversibility in transgenic Arabidopsis thaliana . Plant Cell Physiol. , 36 : 1331 – 1339 .
   PubMed Web of Science ®Google Scholar
• Holowach , L.P. , Madison , J.T. and Thompson , J.F. 1986 . Studies on the mechanism of regulation of the mRNA level for a soybean storage protein subunit by exogenous L-methionine . Plant Physiol. , 80 : 561 – 567 .
   PubMed Web of Science ®Google Scholar
• Janssen , B.-J. and Gardner , R.C. 1989 . Localized transient expression of GUS in leaf discs following cocultivation with Agrobacterium . Plant Mol. Biol. , 14 : 61 – 72 .
   Web of Science ®Google Scholar
• Jaunet , T. , Laguerre , G. , Lemanceau , P. , Frutos , R. and Notteghem , J.L. 1995 . Diversity of Pseudomonas fuscovaginae and other fluorescent pseudomonas isolated from diseased rice . Phytopathology , 85 : 1534 – 1541 .
   Google Scholar
• Jefferson , R.A. 1987 . Assaying chimeric genes in plants: The GUS gene fusion system . Plant Mol. Biol. , 5 : 387 – 405 .
   Google Scholar
• Kapila , J. , Rycke , R.D. , Van Montagu , M. and Angenon , G. 1997 . An Agrobacterium-mediated transient gene expression system for intact leaves . Plant Sci. , 122 : 101 – 108 .
   Web of Science ®Google Scholar
• Lonsdale , D.M. , Allen , R.L. , Belostosky , D. , Ghose , T.K. , Harvey , A.J. , Rogers , H.J. , Tebbut , S.J. and Trick , M. 1995 . An analysis of the relative activities of a number of promoter constructs from genes which are expressed during late pollen development as determined by particle bombardment . Plant Cell Rep. , 15 : 154 – 158 .
   PubMedGoogle Scholar
• Martin , T. , Wöhner , R.-V. , Hummel , S. , Willmitzer , L. and Frommer , W.B. 1992 . “ The GUS reporter system as a tool to study plant gene expression ” . In GUS Protocols: Using the GUS Gene as a Reporter Gene Expression , Edited by: Gallagher , S.R. 23 – 43 . San Diego , California : Academic Press Inc. .
   Google Scholar
• Naleway , J.J. 1992 . “ Histochemical, spectrophotometric, and fiuorometric GUS substrates ” . In GUS Protocols: Using the GUS Gene as a Reporter Gene Expression , Edited by: Gallagher , S.R. 61 – 76 . San Diego , California : Academic Press Inc. .
   Google Scholar
• Sambrook , J. , Fritsh , D.F. and Maniatis , T. 1989 . Molecular Cloning: A Laboratory Manual , New York : Cold Spring Harbor Laboratory Press .
   Google Scholar
• Takahashi , H. , Yamazaki , M. , Sasakura , N. , Watanabe , A. , Leustek , T. , Engler , J.A. , Engler , G. , Van Montagu , M. and Saito , K. 1997 . Regulation of sulfur assimilation in higher plants: A sulfate transporter induced in sulfate-starved roots plays a central role in Arabidopsis thaliana . Proc. Natl. Acad. Sci USA , 94 : 11102 – 11107 .
   PubMed Web of Science ®Google Scholar