Multigram Synthesis of N ^G-Methyl-(L)-Arginine and Its Analytical Characterization

References

• Furchgott , R. F. and Zawadzki , J. V. 1980 . Nature (London) , 288 : 373 – 376 .
   PubMed Web of Science ®Google Scholar
• Palmer , R. M. J. , Ferrige , A. G. and Moncada , S. 1987 . Nature (London) , 327 : 524 – 526 .
   PubMed Web of Science ®Google Scholar
• Ignarro , L. J. , Byrns , R. E. , Buga , G. M. and Wood , K. S. 1987 . Circ. Res. , 61 : 866 – 879 .
   PubMed Web of Science ®Google Scholar
• Ignarro , L. J. , Buga , G. M. , Wood , K. S. , Byrnes , R. E. and Chaudhuri , G. 1987 . Proc. Natl. Acad. Sci. Usa , 84 : 9265 – 9269 .
   PubMed Web of Science ®Google Scholar
• Palmer , R. M. J. , Ashton , D. S. and Moncada , S. 1988 . Nature (London) , 333 : 664 – 666 .
   PubMed Web of Science ®Google Scholar
• Schmidt , H. H. H. W. , Nau , H. , Wittfoht , W. , Gerlach , J. , Prescher , K. E. , Klein , M. N. , Niroomand , F. and Böhme , E. 1988 . Eur. J. Pharmacol. , 154 : 213 – 216 .
   PubMed Web of Science ®Google Scholar
• Sakuma , I. , Stuehr , D. J. , Gross , S. S. , Nathan , C. and Levi , R. 1988 . Proc. Natl. Acad. Sci. Usa , 85 : 8664 – 8667 .
   PubMed Web of Science ®Google Scholar
• Aisaka , K. , Gross , S. S. , Griffith , O. W. and Levi , R. 1989 . Biochem. Biophys. Res. Commun. , 160 : 881 – 886 .
   PubMed Web of Science ®Google Scholar
• Kasai , T. , Samo , M. and Sakamura , S. 1976 . Agric. Biol. Chem. , 40 : 2449 – 2453 .
   Web of Science ®Google Scholar
• Corbin , J. L. and Reporter , M. 1974 . Anal. Biochem. , 57 : 310 – 312 .
   PubMed Web of Science ®Google Scholar
• Patthy , A. , Bajusz , S. and Patthy , L. 1977 . Acta Biochim. Et Biophys. Acad. Sci. Hung. , 12 : 191 – 196 .
   PubMedGoogle Scholar
• Bajusz, Sandoz, Patthy, Andreas Hung. Teljes 8019 (Cl. C 07c), 27 Apr. 1974, Appl. GO-11 89 22 Feb 1972
   Google Scholar
• L-NG-Methylargine monoacetate salt was obtained from Novabiochem (Switzerland) cod.04–11–9022. [α]D20 =+ 10.78 (c=0.7, H2O)
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• HPLC Analyses were carried out on a Hewlett-Packard 1090 liquid chromathograph equipped with a Merck RP-8 Lichrocart endcapped (5μm; 125mmx4mm) column. Eluent: triethylammonium phosphate buffer (pH3.5)/MeOH/iPrOH, 65/25/10
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• TLC (CH3OH-NH4OH 7:3), Rf=0.45 Ninhydrin Spray 0.2% ETOH.
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• [α]D20=+ 23° (C=1, HC1 1N). 1H-NMR (200 MHz(DC1 1N): δ(ppm): 1.70–2.29 (m, 4H); 2.96 (s, 3H); 3.40 (t, 2H); 4.29 (t, 1H); 13C-NMR (50.3 MHz) (DC1 1N): δ(ppm): 174.54; 159.40; 55.13; 42.98; 30.14; 29.48; 26.51
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