Isolation and cultivation of Ca²⁺ tolerant cardiomyocytes from the adult rat: improvements and applications

References

• ALTSCHULD, R. A., GIBB, L., ANSEL, A., HOHL, C., KRUGER, F. A. and BRIERLEY, G. P., 1980, Calcium tolerance of isolated rat heart cells. Journal of Molecular and Cellular Cardiology, 12, 1383–1395.
   PubMedGoogle Scholar
• ARRAS, M., MOLLNAU, H., STRASSER, R., WENZ, R., ITo, W. D., SCHAPER, J. and SCHAPER, W., 1998, The delivery of angiogenic factors to the heart by microsphere therapy. Nature Biotechnology, 16, 159–162.
   PubMed Web of Science ®Google Scholar
• ARTURSSON, P. and MAGNUSSON, C., 1989, Epithelial transport of drugs in cell culture. II: Effect of extracellular calcium concentration on the paracellular transport of drugs of different lipo-philicities across monolayers of intestinal epithelial (CaCo-2) cells. Journal of Pharmaceutical Science, 7, 595–600.
   Google Scholar
• BAHR, M., SPELLEKEN, M., BOCK, M., VON HOLTEY, M., KIEHN, R. and ECKEL, J., 1996, Acute and chronic effects of troglitazone (CS-045) on isolated rat ventricular cardiomyocytes. Diabetologia, 39, 766–774.
   PubMedGoogle Scholar
• BKAILY, G., SPERELAKIS, N. and DOANE, J., 1984, A new method for preparation of isolated single adult myocytes. American Journal of Physiology, 247, 1018–1028.
   Google Scholar
• BRIERLEY, G. P., WENGER, W. C. and ALTSCHULD, R. A., 1985, Heart myocytes as models of the cellular response to ischaemia. Advances in Experimental Medicine and Biology, 194, 303–314.
   Google Scholar
• CAVANAUGH, N. W., 1955, Pulsation, migration and division in dissociated chick embryo heart cells in vitro. Journal of Experimental Zoology, 128, 573–585.
   Google Scholar
• CHARRON, F., PARADIS, P., BRONCHAIN, O., NEMER, G. and NEMER, M., 1999, Cooperative interaction between GATA-4 and GATA-6 regulates myocardial gene expression. Molecular Cell Biology, 19, 4355–4365.
   PubMedGoogle Scholar
• DARSINOS, J. T., SAMOUILIDOU, E. C., KRUMHOLZ, B., KONTOYANNI, M., PESTEVOS, A. K., KARLI, J. N., THEODORAKIS, M. G., LEVIS, G. M. and MOULOPOULOS, S. D., 1993, Distribution of lidocaine and digoxin in heart tissues and aorta following intrapericardial administration. International Journal of Clinical Pharmacology and Therapeutk Toxicology, 31, 611–615.
   PubMed Web of Science ®Google Scholar
• DURAND, J. B., BACHINSKI, L. L., BIELING, L. C., CZERNUSZEWICZ, G. Z., ABCHEE, A. B., Yu, Q. T., TAPSCOTT, T., HILL, R., IFEGWU, J. and MARIAN, A. J., 1995, Localization of a gene responsible for familial dilated cardiomyopathy to chromosome 1q32. Circulation, 92, 3387–3389.
   PubMedGoogle Scholar
• FABIATO, F., FABIATO, A. and SONNENBLICK, E. H., 1971, Isolated myocardial cells of adult mammals. Analytical study of contractility and excitation-contraction coupling. Journal of Physiology (Paris), 63, 210A.
   PubMedGoogle Scholar
• FABIATO, A. and FABIATO, F., 1972, Excitation-contraction coupling of isolated cardiac fibers with disrupted or closed sarcolemmas. Calcium-dependent cyclic and tonic contractions. Circulation Research, 31, 293–307.
   PubMed Web of Science ®Google Scholar
• FANTINI, E., DEMAISON, L., SENTEX, E., GRYNBERG, A. and ATHIAS, P., 1994, Some biochemical aspects of the protective effect of trimetazidine on rat cardiomyocytes during hypoxia and reoxygenation. Journal of Molecular and Cellular Cardiology, 26, 949–958.
   PubMed Web of Science ®Google Scholar
• GEETHA, A., MARAR, T. and DEVI, C. S., 1991, Effect of alpha-tocopherol on doxrubicin-induced changes in rat liver and heart microsomes. Indian Journal of Experimental Biology, 2, 782–785.
   Google Scholar
• GHANBARI, H. A. and MCCARL, R. L., 1980, A comparative study of myosin and its subunits in adult and neonatal-rat hearts and in rat heart cells from young and old cultures. Biochemical journal, 191, 627–635.
   PubMedGoogle Scholar
• GREEN, N. K., FRANKLYN, J. A., OHANIAN, V., HEAGERTY, A. M. and GAMMAGE, M. D., 1997, Transfection of cardiac muscle: effects of overexpression of c-myc and c-fos protooncogene proteins in primary cultures of neonatal rat cardiac myocytes. Clinical Science (Colch), 92, 181–188.
   PubMedGoogle Scholar
• GROSSO, D. S., FRANGAKIS, C. J., CARLSON, E. C. and BRESSLE R., 1977, Isolation and characterisation of myocytes from the adult rat heart. Preparations in Biochemistry, 7, 383–401.
   PubMed Web of Science ®Google Scholar
• HABAZETTL, H., VOIGTLANDER, J., LEIDERER, R. and MESSMER, K., 1998, Efficacy of myocardial initial reperfusion with 2,3 butanedione monoxime after cardioplegic arrest is time-dependent. Cardiovascular Research, 37, 684–690.
   PubMedGoogle Scholar
• HARARY, I. and FARLEY, B., 1960, In vitro studies of single isolated beating heart cells. Science, 131, 1674–1675.
   PubMedGoogle Scholar
• HAWORTH, R. A., GOKNUR, A. B., WARNER, T. F. and BERKOFF, H. A., 1989, Some determinants of quality and yield in the isolation of adult heart cells from rat. Cellular Calcium, 10, 57–62.
   PubMedGoogle Scholar
• HE, S. Y., 1995, Methamphetamine-induced toxicity in cultured adult rat cardiomyocytes. Nippon Hoigaku Zasshi, 49, 175–186.
   PubMedGoogle Scholar
• HERLITZ, J., WALDENSTROM, J. and HJALMARSON, A., 1988, Infarct size limitation after early intervention with metoprolol in the MIAMI trial. Cardiology, 75, 117–122.
   PubMed Web of Science ®Google Scholar
• JANs, S. W., WILLEMS, J., VAN BILSEN, M., REUTELINGSPERGER, C. P. and VAN DER VUSSE, G. J., 1997, Phospholipid degradation in energy-deprived cardiac myocytes: does annexin V play a role? Journal of Molecular and Cellular Cardiology, 29, 1401–1410.
   Google Scholar
• KAO, R. L., CHRISTMAN, E. W., LUH, S. L., KRAUHS, J. M., TYERS, G. F. and WILLIAMS, E. H., 1980, The effects of insulin and anoxia on the metabolism of isolated mature rat cardiac myocytes. Archives in Biochemistry and Biophysics, 203, 587–599.
   PubMedGoogle Scholar
• KATO, Y., MASUMIYA, H., AGATA, N., TANAKA, H. and SHIGENOBU, K. J., 1996, Developmental changes in action potential and membrane currents in foetal, neonatal and adult guinea-pig ventricular myocytes. Journal of Molecular and Cellular Cardiology, 28, 1515–1522.
   PubMedGoogle Scholar
• KESSLER-ICEKSON, G., SCHLESINGER, H., DIALDETTI, M., BRODIE, C., SAMPSON, S. R. and BERGMANN, M., 1996, Cardiomyocytes in culture - a model to study the cellular actions of amiodarone. Israeli Journal of Medical Science, 32, 1212–1216.
   Google Scholar
• KIVISTO, T., MAKIRANTA, M., OIKARINEN, E. L., KARHU, S., WECKSTROM, M. and SELLIN, L. C., 1995, 2,3-Butanedione monoxime (BDM) increases initial yields and improves long-term survival of isolated cardiac myocytes. Japanese Journal of Physiology, 45, 203–210.
   PubMedGoogle Scholar
• KoH, G. Y., SOONPAA, M. H., KLuG, M. G. and FIELD, L. J., 1993, Long-term survival of AT-1 cardiomyocyte grafts in syngeneic myocardium. American Journal of Physiology, 264, 1727–1733.
   Google Scholar
• LAKICH, M. M., DIAGANA, T. T., NORTH, D. L. and WHALEN, R. G., 1998, MEF-2 and Oct-1 bind to two homologous promoter sequence elements and participate in the expression of a skeletal muscle-specific gene. Journal of Biological Chemistry, 273, 15217–15226.
   PubMed Web of Science ®Google Scholar
• LEE, Y., NADAL-GINARD, B., MAHDAVI, V. and Izumo, S., 1997, Myocyte-specific enhancer factor 2 and thyroid hormone receptor associate and synergistically activate the alpha-cardiac myosin heavy-chain gene. Molecular Cell Biology, 17, 2745–2755.
   Google Scholar
• LEOR, J., PRENTICE, H., SARTORELLI, V., QUINONES, M. J., PATTERSON, M., KEDES, L. K. and KLONER, R. A., 1997, Gene transfer and cell transplant: an experimental approach to repair a 'broken heart'. Cardiovascular Research, 35, 431–441.
   PubMed Web of Science ®Google Scholar
• LI, R. K., YAU, T. M., SAKAI, T., MICKLE, D. A. and WEISEL, R. D., 1998, Cell therapy to repair broken hearts. Canadian Journal of Cardiology, 14, 735–744.
   PubMedGoogle Scholar
• LOPEZ JIMENEZ, J. A., BORDONI, A., LORENZINI, A., Rossi, C. A., BIAGI, P. L. and HRELIA, S., 1997, Linoleic acid metabolism in primary cultures of adult rat cardiomyocytes is impaired by aging. Biochemical and Biophysical Research Communications, 237, 142–145.
   PubMedGoogle Scholar
• LOWES, B. D., MINOBE, W., ABRAHAM, W. T., RIZEQ, M. N., BOHLMEYER, T. J., QUAIFE, R. A., RODEN, R. L., DUTCHER, D. L., ROBERTSON, A. D., VOELKEL, N. F., BADESCH, D. B., GROVES, B. M., GILBERT, E. M. and BRISTOW, M. R., 1997, Changes in gene expression in the intact human heart. Downregulation of alpha-myosin heavy chain in hypertrophied, failing ventricular myocardium. Journal of Clinical Investigation, 100, 2315–2324.
   PubMed Web of Science ®Google Scholar
• MARINO, T. A., WALTER, R. A., DAMBRA, K. and MERCER, W. E., 1989, Effects of catecholamines on foetal rat cardiocytes in vitro. American Journal of Anatomy, 186, 127–132.
   PubMedGoogle Scholar
• MARSH, J. D., LEHMANN, M. H., RITCHIE, R. H., GWATHMEY, J. K., GREEN, G. E. and SCHIEBINGER, R. J., 1998, Androgen receptors mediate hypertrophy in cardiac myocytes. Circulation, 98,256–261.
   PubMed Web of Science ®Google Scholar
• MARUYAMA, K., MORI, Y., MURASAWA, S., MASAKI, H., TAKAHASHI, N., TSUTUSUMI, Y., MORIGUCHI, Y., SHIBAZAKI, Y., TANAKA, Y., SHIBUYA, M., INADA, M., MATSUBARA, H. and IWASAKA, T., 1999, Interleukin-1 beta upregulates cardiac expression of vascular endothelin growth factor and its receptor KDR/flk-1 via activation of protein tyrosine kinases. Journal of Molecular and Cellular Cardiology, 31, 607–617.
   PubMedGoogle Scholar
• McCALLum, G. P., HORTON, J. E., FALKNER, K. C. and BEND, J. R., 1993, Microsomal cytochrome P4501A1 dependent monooxygenase activity in guinea pig heart: induction, inhibition, and increased activity by addition of exogenous NADPH-cytochrome P450 reductase. Canadian Journal of Physiology Pharmacology, 71, 151–156.
   PubMed Web of Science ®Google Scholar
• MOLKENTIN, J. D., Lu, J. R., ANTOS, C. L., MARKHAM, B., RICHARDSON, J., ROBBINS, J., GRANT, R. R. and OLSON, E. N., 1998, A calcineurin-dependent transcriptional pathway for cardiac hy-pertrophy. Cell, 93, 215–228.
   PubMed Web of Science ®Google Scholar
• NAG, A. C., CHENG, M., FISCHMAN, D. A. and ZAK, R., 1983, Long-term cell culture of adult mammalian cardiac myocytes: electron microscopic and immuno fluorescent analyses of myofibrillar structure. Journal of Molecular and Cellular Cardiology, 15, 301–317.
   PubMed Web of Science ®Google Scholar
• NAG, A. C., INGLAND M. and CHENG, M., 1985, Factors controlling embryonic heart cell proliferation in serum-free synthetic media. In Vitro Cellular and Developmental Biology, 21, 553–562.
   Google Scholar
• NAG, A. C. and ZAK, R., 1979, Dissociation of adult mammalian heart into single cell suspension: an ultrastructural study. Journal of Anatomy, 129, 541–559.
   PubMedGoogle Scholar
• NAKAO, K., MINOBE, W., RODEN, R., BRISTOW, M. R. and LEINWAND, L. A., 1997, Myosin heavy chain gene expression in human heart failure. Journal of Clinical Investigation, 100, 2362–2370.
   PubMed Web of Science ®Google Scholar
• Nuss, H. B., MARBAN, E. and JOHNS, D. C., 1999, Overexpression of a human potassium channel suppresses cardiac hyperexcitability in rabbit ventricular myocytes. Journal of Clinical Investi-gation, 103, 889–896.
   PubMed Web of Science ®Google Scholar
• ORFALI, K. A., FRYER, L. G., HOLNESS, M. J. and SUGDEN, M. C., 1995, Interactive effects of insulin and triiodothyronine on pyruvate dehydrogenase kinase activity in cardiac myocytes. Journal of Molecular and Cellular Cardiology, 27, 901–908.
   PubMedGoogle Scholar
• PEHLIVAN, T., POBER, B. R., BRUECKNER, M., GARRETT, S., SLAUGH, R., VAN RHEEDEN, R., WILSON, D. B., WATSON, M. S., HING, A. V., 1999, GATA4 haploinsufficiency in patients with interstitial deletion of chromosome region 8p23.1 and congenital heart disease. American Journal of Medical Genetics, 83, 201–206.
   PubMedGoogle Scholar
• PIN, C. L. and MERRIFIELD, P. A.,1993, Embryonic and foetal rat myoblasts express different phenotypes following differentiation in vitro. Developmental Genetics, 14, 356–368.
   PubMedGoogle Scholar
• POWELL, T. and TWIST, V. W., 1976, A rapid technique for the isolation and purification of adult cardiac muscle cells having respiratory control and a tolerance to calcium. Biochemical and Biophysical Research Communications, 72, 327–333.
   PubMed Web of Science ®Google Scholar
• RIVAS, P. A., FABREGA, A. J., SCHWARTZ, D. and POLLAK, R., 1993, The morphology and function of rabbit hepatocytes isolated using ethylendiaminetetraacetate. Transplantation, 55, 335–339.
   PubMedGoogle Scholar
• RUMYANTSEV, P. P., 1963, A morphological and autoradiographical study of the peculiarities of differentiation rate, DNA synthesis and nuclear division in the embryonal and postnatal histogenesis of cardiac muscles of white rats. Histochemica et Cytochemica, 1, 463471.
   Google Scholar
• SAGE, M. D. and JENNINGS, R. B., 1988, Cytoskeletal injury and subsarcolemmal bleb formation in dog heart during in vitro total ischaemia. American Journal of Pathology, 133, 327–337.
   PubMed Web of Science ®Google Scholar
• SCHOTT, J. J., BENSON, D. W., BASSON, C. T., PEASE, W., SILBERBACH, G. M., MOAK, J. P., MARON, B. J., SEIDMANN, C. E., SEIDMANN, J. G., 1998, Congenital heart disease caused by mutations in the transcription factor Nkx2-5. Science, 281, 108–111.
   PubMed Web of Science ®Google Scholar
• SCHWARTZ, K., CHASSAGNE, C. and BOHELER, K. R., 1993, The molecular biology of heart failure. Journal of the American College of Cardiology, 22, 30A–33A.
   Google Scholar
• SEPULVEDA, J. L., BELAGULI, N., NIGAM, V., CHEN, C. Y., NEMER, M. and SCHWARTZ, R. J., 1998, GATA-4 and Nkx2. 5 coactivate Nkx-2 DNA binding targets: role for regulating early cardiac gene expression. Molecular Cell Biology, 18, 3405–3415.
   PubMed Web of Science ®Google Scholar
• SOONPAA, M. H., Kox, G. Y., KLuG, M. G. and FIELD, L. J., 1994, Formation of nascent intercalated disks between foetal cardiomyocytes and host myocardium. Science, 264, 98–101.
   PubMed Web of Science ®Google Scholar
• STEGEMAN, J. J., HAHN, M. E., WEISBROD, R., WOODIN, B. R., JoY, J. S., NAJIBI, S. and COHEN, R. A., 1995, Induction of cytochrome P4501A1 by aryl hydrocarbon receptor agonists in porcine aorta endothelial cells in culture and cytochrome P4501A1 activity in intact cells. Molecular Pharmacology, 47, 296–306.
   PubMed Web of Science ®Google Scholar
• STEGEMAN, J. J., MILLER, M. R. and HINTON, D. E., 1989, Cytochrome P4501A1 induction and localization in endothelium of vertebrate (teleost) heart. Molecular Pharmacology, 36, 723–729.
   PubMed Web of Science ®Google Scholar
• STEGEMAN, J. J., WOODIN, B. R., KLOTZ, A. V., WOLKE, R. E. and ORME-JOHNSON, N. R., 1982, Cytochrome P-450 and monooxygenase activity in cardiac microsomes from the fish Stenotomus chrysops. Molecular Pharmacology, 21, 517–526.
   Google Scholar
• STRINGHAM, J. C., PAULSEN, K. L., SOUTHARD, J. H., FIELDS, B. L. and BELZER, F. O., 1992, Improved myocardial is chaemic tolerance by contractile inhibition with 2,3-butanedione monoxime. Annals of Thoracic Surgery, 54, 859–860.
   Google Scholar
• TAKASAGO, T., GOTO, Y., KAWAGUCHI, O., HATA, K., SAEKI, A., TAYLOR, T. W., NISHIOKA, T. and SUGA, H., 1997, 2,3-Butanedione monoxime suppresses excitation-contraction coupling in the canine blood-perfused left ventricle. Japanese Journal of Physiology, 47, 205–215.
   PubMedGoogle Scholar
• Thum, T. and BORLAK, J., 2000a, Cytochrome P450 monooxygenase gene expression and protein activity in cultures of adult cardiomyocytes of the rat. British Journal of Pharmacology, 130, 1745–1752.
   PubMed Web of Science ®Google Scholar
• Tnum, T. and BORLAK, J., 2000b, Reprogramming of gene expression in cultures of cardiomyocytes and in explanted hearts by the myosin ATPase inhibitor butanedione monoxime. Transplantation (in press).
   Google Scholar
• Tnum, T. and BORLAK, J., 2000c, Gene expression in distinct regions of the heart. Lancet, 355, 979-983. VAHOUNY, G. V., WIE, R., STARKWEATHER, R. and DAVIS, C. M., 1970, Preparation of beating heart cells from adult rats. Science, 167, 1616–1618.
   Google Scholar
• VAN DER HEIDE, R. S., RIM, D., Holm, C. M. and GANOTE, C. E., 1990, An in vitro model of myocardial ischaemia utilising isolated adult rat myocytes. Journal of Molecular and Cellular Cardiology, 22, 165–181.
   PubMedGoogle Scholar
• VANWINKLE, W. B., SNUGGS, M., MILLER, J. C. and Buj-A, L. M., 1994, Cytoskeletal alterations in cultured cardiomyocytes following exposure to the lipid peroxidation product, 4-hydroxynonenaL Cellular Motility and the Cytoskeleton, 28, 119–134.
   Google Scholar
• VERRECCHIA, F. and HERVE, J. C., 1997, Reversible blockade of gap junctional communication by 2,3-butanedione monoxime in rat cardiac myocytes. American Journal of Physiology, 272, 875–885.
   Google Scholar
• WATANABE, E., SMITH, D. M. JR., DELCARPIO, J. B., SUN, J., SMART, F. W., VAN METER, C. H. JR and CLAYCOMB, W. C., 1998, Cardiomyocyte transplantation in a porcine myocardial infarction model. Cell Transplantaticn, 7, 239–246.
   Google Scholar
• WEISENSEE, D., SEEGER, T., BITTNER, A., BEREITER-HAHN, J., SCHOEPPE, W. and LOW-FRIEDRICH, I., 1995, Cocultures of foetal and adult cardiomyocytes yield rhythmically beating rod shaped heart cells from adult hearts. In Vitro Cellular and Developmental Biology, 31, 190–195.
   Google Scholar
• WESTFALL, M. V., RUST, E. M. and METZGER, J. M., 1997, Slow skeletal troponin I gene transfer, expression, and myofilament incorporation enhances adult cardiac myocyte contractile function. Proceeding of the National Academy of Sciences, USA, 94, 5444–5449.
   PubMedGoogle Scholar
• XIAO, Y. F., HUANG, L. and MORGAN, J. P., 1998, Cytochrome P450: a novel system modulating Ca' channels and concentration in mammalian heart cells. Journal of Physiology, 508, 377–392.
   Google Scholar
• YAMADA, H., KANEKO, H., TAKEUCHI, K., OGURI, K. and YOSHIMURA, H., 1992, Tissue-specific expression, induction, and inhibition through metabolic intermediate-complex formation of guinea pig cytochrome P450 belonging to the CYP2B subfamily. Archives in Biochemistry and Biophysics, 299, 248–254.
   PubMed Web of Science ®Google Scholar
• YOSHIMURA, M., YASUE, H., OKUMURA, K., OGAWA, H., JOUGASAKI, M., MUKOYAMA, M., NAKAO, K. and ImuRA, H., 1993, Different secretion patterns of atrial natriuretic peptide and brain natriuretic peptide in patients with congestive heart failure. Circulation, 87, 464–469.
   PubMed Web of Science ®Google Scholar