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Xenobiotica
the fate of foreign compounds in biological systems
Volume 32, 2002 - Issue 8
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Research Article

Permissive and suppressive effects of dexamethasone on enzyme induction in hepatocyte co-cultures

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Pages 653-666 | Published online: 22 Sep 2008

References

  • BADER, A., BOREL RINKES, I. H., CLOSS, E. I., RYAN, C. M., TONER, M., CUNNINGHAM, J. M., TOMPKINS, R. G. and YARMUSH, M. L., 1992, A stable long-term hepatocyte culture system for studies of physiologic processes: cytokine stimulation of the acute phase response in rat and human hepatocytes. Biotechnology Progress, 8, 219–225.
  • BADER, A., KNOP, E., BOKER, K. H., CROME, O., FRUHAUF, N., GONSCHIOR, A. K., CHRISTIANS, U., ESSELMANN, H., PICHLMAYR, R. and SEWING, K. F., 1996, Tacrolimus (FK 506) biotransformation in primary rat hepatocytes depends on extracellular matrix geometry. Naunyn Schmiedebergs Archives of Pharmacology, 353, 461–473.
  • BRADFORD, M. M., 1969, A rapid and sensitive method for the quantification of microgram quantities of protein utilizing the principle of protein—dye binding. Analytical Biochemistry, 27, 502–522.
  • BURCZYNSKI, M. E., MCMILLAN, M., PARKER, J. B., BRYANT, S., LEONE, A., GRANT, E. R., THORNE, J. M., ZHONG, Z., ZIVIN, R. A. and JOHNSON, M. D.., 2001, Cytochrome P450 induction in rat hepatocytes assessed by quantitative real-time reverse-transcription polymerase chain reaction and the RNA invasive cleavage assay. Drug Metabolism and Disposition, 29,1243–1250.
  • BURKE, M. D. and MAYER, R. T., 1974, Ethoxyresorufin: direct fluorimetric assay of a microsomal O.dealkylation which is preferentially by 3-methylcholanthrene. Drug Metabolism and Disposition, 2, 583–588.
  • COONEY, G. F., MOCHON, M., KAISER, B., DUNN, S. P. and GOLDSMITH, B., 1995, Effects of carbamazepine on cyclosporine metabolism in pediatric renal transplant recipients. Pharmacotherap, 15, 353–356.
  • DONATO, M. T., GOMEZ LECHON, M. J. and CASTELL, J. V., 1990, Effect of xenobiotics on monooxygenase activities in cultured human hepatocytes. Biochemical Pharmacology, 39, 1321–1326.
  • Dou, M., DE SOUSA, G., LACARELLE, B., PLACIDI, M., LECHENE DE LA PORTE, P., DOMINGO, M., LAFONT, H. and RAHMANI, R., 1992, Thawed human hepatocytes in primary culture. Cryobiology, 29,454–469.
  • DESouzA, M. J., PoLLocK, S. H. and SOLOMON, H. M., 1988, Cyclosporine-phenytoin interaction. Drug Metabolism and Disposition, 16, 256–258.
  • EDWARDS, D. P., 1999, Coregulatory proteins in nuclear hormone receptor action. Vitamins and hormones, 55, 165–218.
  • EDWARDS, A. M. and LUCAS, C. M., 1985a, Phenobarbital and some other liver tumor promoters stimulate DNA synthesis in cultured rat hepatocytes. Biochemical and Biophysical Research Communications, 131, 103–108.
  • EDWARDS, A. M. and LUCAS, C. M., 1985b, Induction of gamma-glutamyl transpeptidase in primary cultures of normal rat hepatocytes by liver tumor promoters and structurally related compounds. Carcinogenesis, 6, 733–739.
  • FORMAN, B. M., TZAMELI, I., CHOI, H. S., CHEN, J., SIMHA, D., SEOL, W., EVANS, R. M. and MOORE, D. D., 1998, Androstane metabolites bind to and deactivate the nuclear receptor CAR-beta. Nature, 395, 612–615.
  • FREEMAN, D. J., LAUPACIS, A., KEOWN, P. A., STILLER, C. R. and CARRUTHERS, S. G., 1984, Evaluation of cyclosporin-phenytoin interaction with observations on cyclosporin metabolites. British Journal of Clinical Pharmacology, 18, 887–893.
  • HENGSTLER, J. G., RINGEL, M., BIEFANG, K., HAMMEL, S., MILBERT, U., GERL, M., KLEBACH, M., DIENER, B., PLATT, K. L., BOTTGER, T., STEINBERG, P. and OESCH, F., 2000a, Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction. Chemico-Biological Interactions, 125, 51–73.
  • HENGSTLER, J. G., UTESCH, D., STEINBERG, P., PLATT, K. L., DIENER, B., RINGEL, M., SWALES, N., FISCHER, T., BIEFANG, K., GERL, M., BOTTGER, T. and OESCH, F., 2000b, Cryopreserved primary hepatocytes as a constantly available in vitro model for the evaluation of human and animal drug metabolism and enzyme induction. Drug Metabolism Reviews, 32, 81–118.
  • IBER, H., CHEN, Q., SEWER, M. and MORGAN, E. T., 1997, Regulation of hepatic cytochrome P450 2C11 by glucocorticoids. Archives of Biochemistry and Biophysics, 345, 305–310.
  • JEMNITZ, K., VERES, Z., MONOSTORY, K. and VERECZKEY, L., 2000, Glucuronidation of thyroxine in primary monolayer cultures of rat hepatocytes: in vitro induction of UDP-glucuronosyl-tranferases by methylcholanthrene, clofibrate, and dexamethasone alone and in combination. Drug Metabolism and Disposition, 28, 34–37.
  • KENDE, A. S., EBETINO, F. H., DRENDEL, W. B., SUNDARALINGAM, M., GLOVER, E. and POLAND, A., 1985, Structure-activity relationship of bispyridyloxybenzene for induction of mouse hepatic aminopyrine N-demethylase activity. Chemical biological, and X-ray crystallographic studies. Molecular Pharmacology, 28, 445–453.
  • KOCAREK, T. A. and REDDY, A. B., 1998, Negative regulation by dexamethasone of fluvastatin-inducible CYP2B expression in primary cultures of rat hepatocytes: role of CYP3A. Biochemical Pharmacology, 55, 1435–1443.
  • KOEBE, H. G., DUNN, J. C., TONER, M., STERLING, L. M., HUBEL, A., CRAVALHO, E. G., YARMUSH, M. L. and TOMPKINS, R. G., 1990, A new approach to the cryopreservation of hepatocytes in a sandwich culture configuration. Cryobiology, 27, 576–584.
  • KREAMER, B. L., STAECKER, J. L., SAWADA, N., SATTLER, G. L., HSIA, M. T. and PITOT, H. C., 1986, Use of a low-speed, iso-density Percoll centrifugation method to increase the viability of isolated rat hepatocyte preparations. In Vitro Cellular and Developmental Biology, 22, 201–211.
  • LEVIN, W., THOMAS, P. E., RYAN, D. E. and WOOD, A. W., 1987, Isoenzyme specificity of testosterone 7 alpha-hydroxylation in rat hepatic microsomes: is cytochrome P-450a the sole catalyst? Archives of Biochemistry and Biophysics, 258, 630–635.
  • Li, A. P., GORYCKI, P. D., HENGSTLER, J. G., KEDDERIS, G. L., KOEBE, H. G., RAHMANI, R., DE SOUSAS, G., SILVA, J. M. and SKETT, P., 1999, Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel. Chemko-Biological Interactions, 121, 117–123.
  • Li, A. P., MAUREL, P., GOMEZ LECHON, M. J., CHENG, L. C. and JURIMA ROMET, M., 1997, Preclinical evaluation of drug-drug interaction potential; present status of the application of primary human hepatocytes in the evaluation of cytochrome P450 induction. Chemico-Biological Interactions, 107, 5–16.
  • LUBET, R. A., Nims, R. W., MAYER, R. T., CAMERON, J. W. and SCHECHTMAN, L. M., 1985, Measurement of cytochrome P-450 dependent dealkylation of alkoxyphenoxazones in hepatic S9s and hepatocyte homogenates: effects of dicumarol. Mutation Research, 142, 127–131.
  • MADAN, A., DEHAAN, R., MUDRA, D., CARROLL, K., LECLUYSE, E. and PARKINSON, A., 1999, Effect of cryopreservation on cytochrome P-450 enzyme induction in cultured rat hepatocytes. Drug Metabolism and Disposition, 27, 327–335.
  • MURRAY, M., 2000, Glucocorticoid- dependent maintenance of CYP2C11-dependent oxidation in male rat liver in vivo. Human and Experimental Toxicology, 19, 126–131.
  • NEMOTO, N., SAKURAI, J. and FUNAE, Y., 1995, Maintenance of phenobarbital-inducible Cyp2b gene expression in C57BL/6 mouse hepatocytes in primary culture as spheroids. Archives of Biochemistry and Biophysics, 316, 362–369.
  • OESCH, F., WAGNER, H., PLATT, K. L. and ARAND, M., 1992, Improved sample preparation for the testosterone hydroxylation assay using disposable extraction columns. Journal of Chromato-graphy, 582, 232–235.
  • PASCUSSI, J. M., GERBAL-CHALOIN, S., FABRE, J. M., MAUREL, P. and VILAREM, M. J., 2000, Dexamethasone enhances constitutive androstane receptor expression in human hepatocytes: consequences on cytochrome P450 gene regulation. Molecular Pharmacology, 58, 1441–1450.
  • RUNGE, D., KOHLER, C., KOSTRUBSKY, V. E., JAGER, D., LEHMANN, T., RUNGE, D. M., MAY, U., STOLZ, D. B., STROM, S. C., FLEIG, W. E. and MICHALOPOULOS, G. K., 2000, Induction of cytochrome P450 (CYP)1A1, CYP1A2, and CYP3A4 but not of CYP2C9, CYP2C19, multidrug resistance (MDR-1) and multidrug resistance associated protein (MRP-1) by prototypical inducers in human hepatocytes. Biochemical and Biophysical Research Communications, 273, 333–341.
  • RUNGE, D., SCHMIDT, H., CHRIST, B. and JUNGERMANN, K., 1991, Mechanism of the permissive action of dexamethasone on the glucagon-dependent activation of the phosphoenolpyruvate carboxykinase gene in cultured rat hepatocytes. European Journal of Biochemistry, 198, 641–649.
  • SEGLEN, P. O., 1976, Preparation of isolated rat liver cells. In D. M. Prescott (ed.), Methods in Cell Biology (New York: Academic Press), pp. 29–83.
  • SIDHU, J. S., FARIN, F. M. and DMIECINSKI, C. J., 1993, Influence of extracellular matrix overlay on phenobarbital-mediated induction of CYP2B1, 2B2, and 3A1 genes in primary adult rat hepatocyte culture. Archives of Biochemistry and Biophysics, 301, 103–113.
  • SIDHU, J. S. and DMIECINSKI, C. J., 1995, Modulation of xenobiotic-inducible cytochrome P450 gene expression by dexamethasone in primary rat hepatocytes. Pharmacogenetics, 5, 24–36.
  • SIDHU, J. S. and DMIECINSKI, C. J., 1999, Insulin-mediated modulation of cytochrome P450 gene induction profiles in primary rat hepatocyte cultures. Journal of Biochemical and Molecular Toxicology, 13, 1–9.
  • SILVER, G., REID, L. M. and KRAUTER, K. S., 1990, Dexamethasone-mediated regulation of 3-methylcholanthrene-induced cytochrome P-450d mRNA accumulation in primary rat hepatocyte cultures. Journal of Biological Chemistry, 265, 3134–3138.
  • SONDERFAN, A. J., ARLOTTO, M. P., DUTTON, D. R., MCMILLEN, S. K. and PARKINSON, A., 1987, Regulation of testosterone hydroxylation by rat liver microsomal cytochrome P-450. Archives of Biochemistry and Biophysics, 255, 27–41.
  • STEINBERG, P., FISCHER, T., KIULIES, S., BIEFANG, K., PLATT', K. L., DESCH, F., BOTTGER, T., BULITTA, C., KEMPF, P. and HENGSTLER, J., 1999, Drug metabolizing capacity of cryopreserved human, rat, and mouse liver parenchymal cells in suspension. Drug Metabolism and Disposition, 27, 1415–1422.
  • STOLTZ, C., VACHON, M. H., TROTTIER, E., DUBOIS, S., PAQUET, Y. and ANDERSON, A., 1998, The CYP2B2 phenobarbital response unit contains an accessory factor element and a putative glucocorticoid response element essential for conferring maximal phenobarbital responsiveness. Journal of Biological Chemistry, 273, 8528–8536.
  • SUEYOSHI, T., KAWAMOTO, T., ZELKO, I., HONKAKOSKI, P. and NEGISHI, M., 1999, The repressed nuclear receptor CAR responds to phenobarbital in activating the human CYP2B6 gene. Journal of Biological Chemistry, 274, 6043–6046.
  • UTESCH, D., DIENER, B., MOLITOR, E., DESCH, F. and PLATT, K. L., 1992, Characterization of cryopreserved rat liver parenchymal cells by metabolism of diagnostic substrates and activities of related enzymes. Biochemical Pharmacology, 44, 309–315.
  • UTESCH, D. and DESCH, F., 1992, Dependency of the in vitro stabilization of differentiated functions in liver parenchymal cells on the type of cell line used for co-culture. In vitro Cellular and Developmental Biology, 28A, 193–198.
  • WAXMAN, D. J., 1988 Interactions of hepatic cytochromes P-450 with steroid hormones. Regioselectivity and stereospecificity of steroid metabolism and hormonal regulation of rat P-450 enzyme expression. Biochemical Pharmacology, 37, 71–84.
  • WAXMAN, D. J., 1999, P450 gene induction by structurally diverse xenochemicals: central role of nuclear receptors CAR, PXR, and PPAR. Archives of Biochemistry and Biophysics, 369, 11–23.
  • WAXMAN, D. J., Ko, A. and WALSH, C., 1983, Regioselectivity and stereoselectivity of androgen hydroxylations catalyzed by cytochrome P-450 isoenzymes purified from phenobarbital-induced rat liver. Journal of Biological Chemistry, 258, 11937–11947.
  • WAXMAN, D. J., MORRISSEY, J. J., NAIK, S. and JAUREGUI, H. O., 1990, Phenobarbital induction of cytochromes P-450. High-level long-term responsiveness of primary rat hepatocyte cultures to drug induction, and glucocorticoid dependence of the phenobarbital response. Biochemical Journal, 271, 113–119.
  • WORTELBOER, H. M., DE KRUIF, C. A., VAN TEASEL, A. A., FALKE, H. E., NOORDHOEK, J. and BLAAUBOER, B. J., 1991, Comparison of cytochrome P450 isoenzyme profiles in rat liver and hepatocyte cultures. The effects of model inducers on apoproteins and biotransformation activities. Biochemical Pharmacology, 42, 381–390.

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