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Xenobiotica
the fate of foreign compounds in biological systems
Volume 32, 2002 - Issue 9
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Research Article

Use of a quantitative real-time reverse transcription-polymerase chain reaction method to study the induction of CYP1A, CYP2B and CYP4A forms in precision-cut rat liver slices

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Pages 739-747 | Published online: 22 Sep 2008

References

  • BARS, R. G., BELL, D. R. and ELCOMBE, C. R., 1993, Induction of cytochrome P450 and peroxisomal enzymes by clofibric acid in vivo and in vitro. Biochemical Pharmacology, 45, 2045–2053.
  • BARS, R. G., MITCHELL, A. M., WOLF, C. R. and ELCOMBE, C. R., 1989, Induction of cytochrome P-450 in cultured rat hepatocytes. The heterogeneous localization of specific isoenzymes using immunocytochemistry. Biochemical Journal, 262, 151–158.
  • BEAMAND, J. A., PRICE, R. J., CUNNINGHAME, M. E. and LAKE, B. G., 1993, Culture of precision-cut liver slices: effect of some peroxisome proliferators. Food and Chemical Toxicology, 31, 137–147.
  • BOWEN, W. P., CAREY, J. E., MI, A., MCMURRAY, H. F., MUNDAY, P. W., JAMES, R. S., COLEMAN, R. A. and BROWN, A. M., 2000, Measurement of cytochrome P450 gene induction in human hepatocytes using quantitative real-time reverse transcriptase-polymerase chain reaction. Drug Metabolism and Disposition, 28, 781–788.
  • BURCZYNSKI, M. E., MCMILLIAN, M., PARKER, J. B., BRYANT, S., LEONE, A., GRANT, E. R., THORNE, J. M., ZHONG, Z., ZIVIN, R. A. and JOHNSON, M. D., 2001, Cytochrome P450 induction in rat hepatocytes assessed by quantitative real-time reverse-transcription polymerase chain reaction and the RNA invasive cleavage assay. Drug Metabolism and Disposition, 29, 1243–1250.
  • CONNEY, A. J., 1986, Induction of microsomal cytochrome P-450 enzymes: the first Bernard B. Brodie lecture at Pennsylvania State University. Life Sciences, 39, 2493–2518.
  • DAVILA, J. C. and MORRIS, D. L., 1999, Analysis of cytochrome P450 and phase II conjugating enzyme expression in adult male rat hepatocytes. In Vitro Cellular and Developmental Biology, 35, 120–130.
  • DRAHUSHUK, A. T., MCGARRIGLE, B. P., TAI, H.-L. KITAREEWAN, S., GOLDSTEIN, J. A. and OLSON, J. R, 1996, Validation of precision-cut liver slices in dynamic organ culture as an in vitro model for studying CYP1A1 and CYP1A2 induction. Toxicology and Applied Pharmacology, 140, 393–403.
  • GIBSON, U. E. M., HEID, C. A. and WILLIAMS, P. M., 1996, A novel method for real-time quantitative PCR. Genome Research, 6, 995–1001.
  • GOKHALE, M. S., BUNTON, T. E., ZURLO, J. and YAGER, J. D., 1995, Cytochrome P-450 1A1/1A2 induction, albumin secretion, and histological changes in cultured liver slices. In vitro Toxicology, 8, 357–368.
  • GRASSO, P. and HINTON, R. H., 1991, Evidence for and possible mechanisms of non-genotoxic carcinogenesis in rodent liver. Mutation Research, 248, 271–290.
  • HEID, C. A., STEVENS, J., LIVAK, K. J. and WILLIAMS, P. M., 1996, Real-time quantitative PCR. Genome Research, 6, 986–994.
  • LAKE, B. G., 1995, Mechanisms of hepatocarcinogenicity of peroxisome-proliferating drugs and chemicals. Annual Review of Pharmacology and Toxicology, 35, 483–507.
  • LAKE, B. G., BEAMAND, J. A., JAPENGA, A. C., RENWICK, A., DAVIES, S. and PRICE, R. J., 1993, Induction of cytochrome P-450-dependent enzyme activities in cultured rat liver slices. Food and Chemical Toxicology, 31, 377–386.
  • LAKE, B. G., CHARZAT, C., TREDGER, J. M., RENWICK, A. B., BEAMAND, J. A. and PRICE, R. J., 1996, Induction of cytochrome P450 isoenzymes in cultured precision-cut rat and human liver slices. Xenobiotica, 26, 297–306.
  • LAKE, B. G. and LEWIS, D. F. V., 1996, The CYP4 family. In C. Ioannides (ed.), Cytochromes P450: Metabolic and Toxicological Aspects (Boca Raton: CRC Press), pp. 271–297.
  • LAKE, B. G., PRICE, R. J., RENWICK, A. B. and BEAMAND, J. A., 1998, Maintenance of intracellular organization in liver slices: importance in studies of drug metabolism. In A. R. Boobis, P. Kremers, O. Pelkonen and K. Pithan (eds), COST B1 European Symposium on the Prediction of Drug Metabolism in Man: Progress and Problems (Luxembourg: European Community), pp. 61–76.
  • LECLUYSE, E. L., BULLOCK, P. L. and PARKINSON, A., 1996, Strategies for restoration and maintenance of normal hepatic structure and function in long-term cultures of rat hepatocytes. Advanced Drug Delivery Reviews, 22, 133–186.
  • LERCHE-LANGRAND, C. and TOUTAIN, H. J., 2000, Precision-cut liver slices: characteristics and use for in vitro pharmaco-toxicology. Toxicology, 153, 221–253.
  • LOWRY, O. H., ROSEBROUGH, N. J., FAR, A. L. and RkNDALL, R. J., 1951, Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.
  • MULLER, D, GLOCKNER, R., ROST, M. and STEINMETZER, P., 1998, Monooxygenation, cytochrome P450-mRNA expression and other functions in precision-cut rat liver slices. Experimental Toxicology and Pathology, 50, 507–513.
  • MULLER, D., STEINMETZER, P., PISSOWOTZKI, K. and GLOCKNE R., 2000, Induction of cytochrome P450 2B1-mRNA and pentoxyresorufin O-depentylation after exposure of precision-cut rat liver slices to phenobarbital. Toxicology, 44, 93–97.
  • MURPHY, L. D., HERZOG, C. E., RUDICK, J. B., Foy), A. T. and BATES, S. E., 1990, Use of the polymerase chain reaction in the quantitation of mdr-1 gene expression. Biochemistry, 29, 10351–10356.
  • NAKAMURA, T., SAKAEDA, T., OHMOTO, N., TAMURA, T., AOYAMA, N., SHIRAKAWA, T., KAMIGAKI, T., NAKAMURA, T., Kim, K. I., Kim, S. R., KURODA, Y., MATSUO, M., KAsuGA, M. and OKUMURA, K., 2002, Real-time quantitative polymerase chain reaction for MDR1, MRP1, MRP2, and CYP3A-mRNA levels in Caco-2 cell lines, human duodenal enterocytes, normal colorectal tissues, and colorectal adenocarcinomas. Drug Metabolism and Disposition, 30, 4–6.
  • NELSON, D. R., KOYMANS, L., KAMATAKI, T., STEGEMAN, J. J., FEYEREISEN, R., WAXMAN, D. J., WATERMAN, M. R., GOTOH, O., COON, M. J., ESTABROOK, R. W., GUNSALUS, I. C. and NEBERT, D. W., 1996, P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics, 6, 1–42.
  • NIMS, R. W., DEVOR, D. E., HENNEMAN, J. R. and LUBET, R. A., 1987, Induction of alkoxyresorufin O-dealkylases, epoxide hydrolase, and liver weight gain: correlation with tumor-promoting potential in a series of barbiturates. Carcinogenesis, 8, 67–71.
  • NIMS, R. W. and LUBET, R. A., 1996, The CYP2B subfamily. In C. Ioannides (ed.), Cytochromes P450: Metabolic and Toxicological Aspects (Boca Raton: CRC press), pp. 135–160.
  • OKEY, A. B., 1990, Enzyme induction in the cytochrome P-450 system. Pharmacology and Therapeutics, 45, 241–298.
  • PAN, J., XIANG, Q. and BALL, S., 2000, Use of a novel real-time quantitative reverse transcription-polymerase chain reaction method to study the effects of cytokines on cytochrome P450 mRNA expression in mouse liver. Drug Metabolism and Disposition, 28, 709–713.
  • PARKINSON, A., 1996, Biotransformation of xenobiotics. In C. D. Klaassen (ed.), Casarett and Doull's Toxicology: The Basic Science of Poisons, 5th edn (New York: McGraw-Hill), pp. 113–186.
  • PRICE, R. J., RENWICK, A. B., BARTON, P. T., HOUSTON, J. B. and LAKE, B. G., 1998, Influence of slice thickness and culture conditions on the metabolism of 7-ethoxycoumarin in precision-cut rat liver slices. Alternatives to Laboratory Animals, 26, 541–548.
  • WHYSNER, J., Ross, P. M. and WILLIAMS, G. M., 1996, Phenobarbital mechanistic data and risk assessment: enzyme induction, enhanced cell proliferation, and tumor promotion. Pharmacology and Therapeutics, 71, 153–191.
  • WIESNER, R., RUEGG, J. C. and MORANO, I., 1992, Counting target molecules by exponential polymerase chain reaction: copy number of mitochondrial DNA in rat tissues. Biochemical and Biophysical Research Communications, 183, 553–559.

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