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Xenobiotica
the fate of foreign compounds in biological systems
Volume 33, 2003 - Issue 5
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Research Article

Constitutive and inducible expression of CYP enzymes in immortal hepatocytes derived from SV40 transgenic mice

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Pages 459-473 | Published online: 22 Sep 2008

References

  • ANTOINE, B., LEVRAT, F., VALLET, V., BERBAR, T., CARTIE, N., DUBOIS, N., BRIAND, P. and KAHN, A., 1992, Gene expression in hepatocyte-like lines established by targeted carcinogenesis in transgenic mice. Experimental Cell Research, 200, 175–185.
  • BAYAD, J., BAGREL, D., SABOLOVIC, N., MAGDALOU, J. and SIEST, G., 1991, Expression and regulation of drug metabolizing enzymes in an immortalized rat hepatocyte cell line. Biochemical Pharmacology, 42, 1345–1351.
  • BORT, R., MACE, K., BOONIS, A., GômEz-LEcHôN, M. J., PFEIFER, A. and CASTELL, J. V., 1999, Hepatic metabolism of diclofenac: role of human CYP in the minor oxidative pathways. Biochemical Pharmacology, 58, 787–796.
  • BURKE, M. D., THOMPSON, S., ELCOMBE, C. R., HALPERT, J., HAAPARANTA, T. and MAYER, R. T., 1985, Ethoxy-, pentoxy-, and bezyloxyphenoxazones and homologues: a series of substrates to distinguish between different induced cytochromes P-450. Biochemical Pharmacology, 34, 3337–3345.
  • COURJAULT-GAUTIER, F., ANTOINE, B., BENS, M., VALLET, V., CLUZEAUD, F., PRINGAULT, E., KAHN, A., TOURAIN, H. and VANDEWALLE, A., 1997, Activity and inducibility of drug-metabolizing enzymes in immortalized hepatocyte-like cells (mhPKT) derived from a L-PK/Tag 1 transgenic mouse. Experimental Cell Research, 234, 362–372.
  • DICKER, E., McHuGH, T. and CEDERBAUM, A. I., 1990, Increased oxidation of p-nitrophenol and aniline by intact hepatocytes isolated from pyrazole-treated rats. Biochimica et Biophysica Acta, 1035, 249–256.
  • DONATO, M. T., BASSI, A. M., GômEz-LEcHôN, M. J., PENCO, S., HERRERO, E., ADAMO, D., CASTELL, J. V. and FERRO, M., 1994a, Evaluation of the xenobiotic biotransformation capability of six rodent hepatoma cell lines in comparison with rat hepatocytes. In Vitro Cellular and Developmental Biology, 30A, 574–580.
  • DONATO, M. T., CASTELL, J. V. and GômEz-LEcHôN, M. J., 1994b, Cytochrome P450 activities in pure and co-cultured rat hepatocytes. Effects of model inducers. In Vitro Cellular and Developmental Biology, 30A, 825–832.
  • DONATO, M. T., CASTELL, J. V. and GômEz-LEcHôN, M. J., 1995, Effect of model inducers 011 cytochrome P450 activities of human hepatocytes in primary culture. Drug Metabolism and Disposition, 23, 553–558.
  • DONATO, M. T., CASTELL, J. V. and GômEz-LEcHôN, M. J., 1999, Characterization of drug metabolizing activities in pig hepatocytes for use in bioartificial liver devices: comparison with other hepatic cellular models. Journal of Hepatology, 31, 542–549.
  • DONATO, M. T., GOmEz-LEcHON, M. J. and CASTELL, J. V., 1993, A microassay for measuring cytochrome P450IA1 and P450IIB1 activities in intact human and rat hepatocytes cultured on 96-well plates. Analytical Biochemistry, 213, 29–33.
  • DONATO, M. T., VIITALA, P., RODRIGUEZ-ANTONA, C., LINDFORS, A., CASTELL, J. V., GOMEZ-LECHÔN, M. J. and PELKONEN, O., 2000, CYP2A5/CYP2A6 expression in mouse and human hepatocytes treated with various in vivo inducers. Drug Metabolism and Disposition, 28, 1321–1326.
  • GOKHALE, M. S., BUNTON, T. E., ZURLO, J. and YAGER, J. D., 1997, Cytochrome P450 isoenzyme activities in cultured rat and mouse liver slices. Xenobiotica, 27, 341–355.
  • GômEz-LEcHôN, M. J., DONATO, T., PONSODA, X., FABRA, R., TRULLENQUE, R. and CASTELL, J. V., 1997, Isolation, culture and use of human hepatocytes in drug research. In J. V. Castell and M. J. Górnez-LEcHôN (eds), In Vitro Methods in Pharmaceutical Research (New York: Academic Press), pp. 129–153.
  • GômEz-LEcHôN, M. J., JovER, R., DONATO, T., PONSODA, X., RODRIGUEZ, C., STENZEL, K. G., KLOCKE, R., PAUL, D., GUILLEN, I., BORT, R. and CASTELL, J. V., 1998, Long-term expression of differentiated functions in hepatocytes cultured in three-dimensional collagen matrix. Journal of Cellular Physiology, 177, 553–562.
  • GOmEz-LEcHON, M. J., LOPEZ, M. P. and CASTELL, J. V., 1984, Biochemical functionality and recovery of hepatocytes after deep-freezing storage. In Vitro, 20, 826–832.
  • GOmEz-LEcHON, M. J., LOPEZ, P., DONATO, T., MONTOYA, A., LARRAURI, A., GIMENEZ, P., TRULLENQUE, R., FABRA, R. and CASTELL, J. V., 1990, Culture of human hepatocytes from small surgical biopsies. Biochemical characterization and comparison with in vivo. In Vitro Cellular and Developmental Biology, 26, 67–74.
  • GRANT, M. H., DUTHIE, S. J., GRAY, A. G. and BURKE, M. D., 1988, Mixed function oxidase and UDP-glucuronyltransferase activities in the human HepG2 hepatoma cell line. Biochemical Pharmacology, 37, 4111–4116.
  • GUENGERICH, F. P., 1999, Cytochrome P-450 3A4: regulation and role in drug metabolism. Annual Review of Pharmacology and Toxicology, 39, 1–17.
  • GuiLLouzo, A., 1998, Liver cell models in in vitro toxicology hepatocytes. Environmental Health Perspectives, 106, 511–531.
  • HOFFMANN, B., PIASECKI, A. and PAUL, D., 1989, Proliferation of fetal rat hepatocytes in response to growth factors and hormones in primary culture. Journal of Cellular Physiology, 139, 654–662.
  • HONKAKOSKI, P., AURIOLA, S. and LANG, M. A., 1992, Distinct induction profiles of three phenobarbital-responsive mouse liver cytochrome P450 isozymes. Biochemical Pharmacology, 43, 2121–2128.
  • Iciiii(AwA, T., ITAKURA, T. and NEGISHI, M., 1989, Functional characterization of two cytochrome p-450s within the mouse, male-specific steroid 16 alpha-hydroxylase gene family: expression in mammalian cells and chimeric proteins. Biochemistry, 28, 4779–4784.
  • IWASAKI, M., LINDBERG, R. L. P., JUVONEN, R. O. and NEGISHI, M., 1993, Site-directed mutagenesis of mouse steroid 7a-hydroxylase (cytochrome P-4507,): role of residue-209 in determining steroid-cytochrome P-450 interaction. Biochemical Journal, 291, 569–573.
  • KLOCKE, R., GômEz-LEcHôN, M. J., EHRHARDT, A., MENDOZA-FIGUEROA, T., DONATO, M. T., LOPEZ-SEVILLA, R., CASTELL, J. V. and PAUL, D., 2002, Establishment and characterization of immortal hepatocytes derived from various transgenic mouse lines. Biochemical and Biophysical Communications, 294, 864–871.
  • KONO, Y., YANG, S., LETARTE, M. and ROBERTS, E. A., 1995, Establishment of a human hepatocyte line derived from primary culture in a collagen gel sandwich culture system. Experimental Cell Research, 221, 478–485.
  • KOOP, D. R., 1992, Oxidative and reductive metabolism by cytochrome P450 2E1. FASEB Journal, 6, 724–730.
  • LINDBERG, R., BURKHART, B., ICHIKAWA T. and NEGISHI, M., 1989, The structure and characterization of type I P-45015a gene as major steroid 15a-hydroxylase and its comparison with type II P-45015a gene. Journal of Biological Chemistry, 264, 6465–6471.
  • LOWRY, O. H., ROSENBROUGH, N. J., FARR, A. L. and RANDALL, R. J., 1951, Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.
  • NERURKAR, P. V., PARK, S. S., THOMAS, P. E., Nims, R. W. and LUBET, R. A., 1993, Methoxyresorufin and benzyloxyresorufin: substrates preferentially metabolized by cytochromes P4501A2 and 2B, respectively, in the rat and mouse. Biochemical Pharmacology, 46, 933–943.
  • PALMITER, R. D., CHEN, H. Y., MESSING, A. and BRINSTER, R. L., 1985, SV 40 enhancer and large T antigen one instrumental in development of choroid plexus tumours in transgenic mice. Nature, 316, 457–460.
  • PAUL, D., WHINE, M., PINKERT, C., PIASECKI, A., UMMELMANN, E. and BRINSTER, R. L., 1988, Immortalized differentiated hepatocyte lines derived from transgenic mice harboring 5V40 T-antigen genes. Experimental Cell Research, 175, 354–362.
  • PETER, R., WICKER, R., BEAUNE, P. H., IWASAKI, M., GUENGERICH, F. P. and YANG, C. S., 1990, Hydroxylation of chlorzoxazone as a specific probe for human liver cytochrome P-450IIE1. Chemical Research in Toxicology, 3, 566–573.
  • PFEIFER, A. M., COLE, K. E., SMOOT, D. T., WASTON, A., GROOPMAN, J. D., SHIELDS, P., VIGNAUD, J. M., JUILLERAT, M., LIPSKY, M. M., TRUMP, B. F., LECHNER, J. F. and HARRIS, C. C., 1993, Simian virus 40 large tumor antigen-immortalized normal human liver epithelial cells express hepatocyte characteristics and metabolize chemical carcinogens. Proceedings of the National Academy of Sciences, USA, 90, 5123–5127.
  • ROBERTS, E. A., LETARTE, M., SQUIRE, J. and YANG, S., 1994 Characterization of human hepatocyte lines derived from normal liver tissue. Hepatology, 19, 1390–1399.
  • RODRIGUES, A. D., 1999, Integrated cytochrome P450 reaction phenotyping. Attempting to bridge the gap between cDNA-expressed cytochrome P450 and native human liver microsomes. Biochemical Pharmacology, 57, 465–480.
  • RODRIGUEZ-ANTONA, C., DONATO, M. T., BOOB'S, A., EDWARDS, R. J., WATTS, P. S., CASTELL, J. V. and GOmEz-LEcHON, M. J., 2002, Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica, 32, 505–520.
  • SAKUMA, T., TAKAI, M., ENDO, Y., KUROIWA, M., OHARA, A., JARUKAMJORN, K., HONNA R. and NEMOTO, N., 2000, A novel female-specific member of the CYP3A gene subfamily in the mouse liver. Archives of Biochemistry Biophysics, 377, 153–162.
  • SCHIPPERS, I. J., MOSHAGE, H., ROELOFSEN, H., MULLER, M., HEYMANS, H. A. S., RUITERS, M. and KUIPERS, F., 1997, Immortalized human hepatocytes as a tool for the study of hepatocytic (de-)differentiation. Cell Biology and Toxicology, 13, 375–386.
  • SHARMA, M. C. and SHAPIRO, B. H., 1995, Purification and characterization of constituent testosterone 2a-hydroxylase (cytochrome P4502a) from mouse liver. Archives of Biochemistry and Biophysics, 316, 478–484.
  • SWALES, N. J., JOHNSON, T. and CALDWELL, J., 1996, Cryopreservation of rat and mouse hepatocytes. II. Assessment of metabolic capacity using testosterone metabolism. Drug Metabolism and Disposition, 24, 1224–1230.
  • TASSANEEYAKUL, W., VERONESE, M. E., BIRKETT, D. L., GONZALEZ, F. J. and MINERS, J. O., 1993, Validation of 4-nitrophenol as an in vitro substrate probe for human liver CYP2E1 using cDNA-expression and microsomal kinetic techniques. Biochemical Pharmacology, 46, 1975–1981.
  • TONJES, R. R., LOHLER, J., O'SULLIVAN, J. F., KAY, G. F., SCHMIDT, G. H., DALEMANS, W., PAVIRONI, A. and PAUL, D., 1995, Autocrine mitogen IgEGF cooperates with c-myc or with the Hcs locus during hepatocarcinogenesis in transgenic mice. Oncogene, 10, 765–768.
  • YANAI, N., SuzuKi, M. and OBINATA, M., 1991, Hepatocyte cell lines established from transgenic mice harboring temperature-sensitive simian virus 40 large T-antigen gene. Experimental Cell Research, 197, 50–56.

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