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Xenobiotica
the fate of foreign compounds in biological systems
Volume 33, 2003 - Issue 6
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Research Article

Functional characterization of three human cytochrome P450 2E1 variants with amino acid substitutions

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Pages 575-586 | Published online: 22 Sep 2008

References

  • ALBANO, E., CLOT, P., MORIMOTO, M., TOMASI, A., INGELMAN-SUNDBERG, M. and FRENCH, S. W., 1996, Role of cytochrome P4502E1-dependent formation of hydroxyethyl free radical in the development of liver damage in rats intragastrically fed with ethanol. Hepatology, 23, 155–163.
  • BROCKMOLLER, J., CASCORBI, I., KERB, R. and ROOTS, I., 1996, Combined analysis of inherited polymorphisms in arylamine N-acetyltransferase 2, glutathione S-transferases M1 and Ti, microsomal epoxide hydrolase, and cytochrome P450 enzymes as modulators of bladder cancer risk. Cancer Research, 56, 3915–3925.
  • DUPONT, I., BODENEZ, P., BERTHOU, F., SIMON, B., BARDOU, L. G. and LUCAS, D., 2000, Cytochrome P-450 2E1 activity and oxidative stress in alcoholic patients. Alcohol and Alcoholism, 35, 98–103.
  • EKINS, S., MAENPAA, J. and WRIGHTON, S. A., 1999, In vitro metabolism: subcelluar fractions. In T. F. Woolf (ed.), Handbook of Drug Metabolism (New York: Marcel Dekker), pp. 363–399.
  • FAIRBROTHER, K. S., GROVE, J., DE WAZIERS, I., STEIMEL, D. T., DAY, C. P., CRESPI, C. L. and DALY, A. K., 1998, Detection and characterization of novel polymorphisms in the CYP2E1 gene. Pharmacogenetics, 8, 543–552.
  • FREEMAN, J. E., STIRLING, D., RUSSELL, A. L. and WOLF, C. R., 1992, cDNA sequence, deduced amino acid sequence, predicted gene structure and chemical regulation of mouse Cyp2el. Biochemical Journal, 281, 689–695.
  • FUKUDA, T., NISHIDA, Y., IMAOKA, S., HIROI, T., NAOHARA, M., FUNAE, Y. and AZUMA, J., 2000, The decreased in vivo clearance of CYP2D6 substrates by CYP2D6*10 might be caused not only by the low-expression but also by low affinity of CYP2D6. Archives of Biochemistry and Biophysics, 380, 303–308.
  • GONZALEZ, F. J., 1990, Molecular genetics of the P-450 superfamily. Pharmacological and Therapeutics, 45, 1–38.
  • GUENGERICH, F. P. and SHIMADA, T., 1991, Oxidation of toxic and carcinogenic chemicals by human cytochrome P-450 enzymes. Chemical Research in Toxicology, 4, 391–407.
  • HU, Y., HAKKOLA, J., OSCARSON, M. and INGELMAN-SUNDBERG, M., 1999, Structural and functional characterization of the 5'-flanking region of the rat and human cytochrome P450 2E1 genes: identification of a polymorphic repeat in the human gene. Biochemical and Biophysical Research Communications, 263, 286–293.
  • HU, Y., OSCARSON, M., JOHANSSON, I., YUE, Q. Y., DAHL, M. L., TABONE, M., ARINCO, S., ALBANO, E. and INGELMAN-SUNDBERG, M., 1997, Genetic polymorphism of human C YP2E1: characterization of two variant alleles. Molecular Pharmacology, 51, 370–376.
  • JOHANSSON, I., OSCARSON, M., YUE, Q. Y., BERTILSSON, L., SJOQVIST, F. and INGELMAN-SUNDBERG, M., 1994, Genetic analysis of the Chinese cytochrome P450 2D locus: characterization of variant CYP2D6 genes present in subjects with diminished capacity for debrisoquine hydroxylation. Molecular Pharmacology, 46, 452–459.
  • KHANI, S. C., PORTER, T. D., FUJITA, V. S. and COON, M. J., 1988, Organization and differential expression of two highly similar genes in the rabbit alcohol-inducible cytochrome P-450 subfamily. Journal of Biological Chemistry, 263, 7170–7175.
  • KIM, R. B. and O'SHEA, D., 1995, Interindividual variability of chlorzoxazone 6-hydroxylation in men and women and its relationship to CYP2E1 genetic polymorphisms. Clinical Pharmacology and Therapeutics, 57, 645–655.
  • KISS, I., SANDOR, J., PAJKOS, G., BOGNER, B., HEGEDUS, G. and EMBER, I., 2000, Colorectal cancer risk in relation to genetic polymorphism of cytochrome P450 1A1, 2E1, and glutathione-S-transferase M1 enzymes. Anticancer Research, 20, 519–522.
  • KOMORI, M., KIKUCHI, O., SAKUMA, T., FUNAKI, J., KITADA, M. and KAMATAKI, T., 1992, Molecular cloning of monkey liver cytochrome P-450 cDNAs, similarity of the primary sequences to human cytochromes P-450. Biochimica et Biophysica Acta, 1171, 141–146.
  • LAEMMLI, U. K., 1970, Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature, 227, 680–685.
  • LIEBER, C. S., 1988, Biochemical and molecular basis of alcohol-induced injury to liver and other tissues. New England Journal of Medicine, 319, 1639–1650.
  • LOWRY, O. H., ROSEBROUGH, N. J., FARR, A. L. and RANDALL, R. J., 1951, Protein measurement with the Folin phenol reagent. Journal of Biological Chemistry, 193, 265–275.
  • LUCAS, D., MENEZ, J. F. and BERTHOU, F., 1996, Chlorzoxazone: an in vitro and in vivo substrate probe for liver CYP2E1. Methods in Enzymology, 272, 115–123.
  • LUCAS, D., MENEZ, C., GIRRE, C., BERTHOU, F., BODENEZ, P., JOANNET, I., HISPARD, E., BARDOU, L. G. and MENEZ, J. F., 1995, Cytochrome P450 2E1 genotype and chlorzoxazone metabolism in healthy and alcoholic Caucasian subjects. Pharmacogenetics, 5, 298–304.
  • MCCARVER, D. G., BYUN, R., HINES, R. N., HICHME, M. and WEGENEK, W., 1998, A genetic polymorphism in the regulatory sequences of human CYP2E1: association with increased chlorzoxazone hydroxylation in the presence of obesity and ethanol intake. Toxicology and Applied Pharmacology, 152, 276–281.
  • NELSON, D. R., KOYMANS, L., KAMATAKI, T., STEGEMAN, J. J., FEYEREISEN, R., WAXMAN, D. J., WATERMAN, M. R., GOTOH, O., COON, M. J., ESTABROOK, R. W., GUNSALUS, I. C. and NEBERT, D. W., 1996, P450 superfamily: update on new sequences, gene mapping, accession numbers and nomenclature. Pharmacogenetics, 6, 1–42.
  • NOVAK, R. F. and WOODCROFT, K. J., 2000, The alcohol-inducible form of cytochrome P450 (CYP 2E1): role in toxicology and regulation of expression. Archives of Pharmacology Research, 23, 267–282.
  • RENDIC, S. and DI CARLO, F. J., 1997, Human cytochrome P450 enzymes: a status report summarizing their reactions, substrates, inducers, and inhibitors. Drug Metabolism Reviews, 29, 413–580.
  • RONIS, M. J. J., LINDROS, K. O. and INGELMAN-SUNDBERG, M., 1996, The CYP2E subfamily: subcelluar fractions. In C. Ioannides (ed.), Cytochrome P450: Methods and Toxicology Aspects (London: CRC Press), pp. 211–239.
  • SHIMADA, T., TSUMURA, F. and YAMAZAKI, H., 1999, Prediction of human liver microsomal oxidations of 7-ethoxycoumarin and chlorzoxazone with kinetic parameters of recombinant cytochrome P-450 enzymes. Drug Metabolism and Disposition, 27, 1274–1280.
  • SHIMADA, T., YAMAZAKI, H., MIMURA, M., INui, Y. and GUENGERICH, F. P., 1994, Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. Journal of Pharmacology and Experimental Therapeutics, 270, 414–423.
  • SONG, B. J., 1996, Ethanol-inducible cytochrome P450 (CYP2E1): biochemistry, molecular biology and clinical relevance: 1996 update. Alcoholism Clinical and Experimental Research, 20, 138A–146A.
  • SONG, B. J., GELBOIN, H. V., PARK, S. S., YANG, C. S. and GONZALEZ, F. J., 1986, Complementary DNA and protein sequences of ethanol-inducible rat and human cytochrome P-450s: transcriptional and post-transcriptional regulation of the rat enzyme. Journal of Biological Chemistry, 261, 16689–16697.
  • TAN, W., SONG, N., WANG, G. Q., Liu, Q., TANG, H. J., KADLUBAR, F. F. and LIN, D. X., 2000, Impact of genetic polymorphisms in cytochrome P450 2E1 and glutathione S-transferases M1, Ti, and P1 on susceptibility to esophageal cancer among high-risk individuals in China. Cancer Epidemiology, Biomarkers and Prevention, 9, 551–556.
  • TANAKA, E., TERADA, M. and MISAWA, S., 2000, Cytochrome P450 2E1: its clinical and toxicological role. Journal of Clinical Pharmacy and Therapeutics, 25, 165–175.
  • TASSANEEYAKUL, W., VERONESE, M. E., BIRKETT, D. J. and MINERS, J. O., 1993, High-performance liquid chromatographic assay for 4-nitrophenol hydroxylation, a putative cytochrome P-450 2E1 activity, in human liver microsomes. Journal of Chromatography, 616, 73–78.
  • TOWBIN, H., STAEHELIN, T. and GORDON, J., 1979, Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proceedings of the National Academy of Sciences, USA, 76, 4350–4354.
  • UMENO, M., MCBRIDE, O. W., YANG, C. S., GELBOIN, H. V. and GONZALEZ, F. J., 1988, Human ethanol-inducible P450IIE1: complete gene sequence, promoter characterization, chromosome mapping, and cDNA-directed expression. Biochemistry, 27, 9006–9013.
  • YOO, J. S., GUENGERICH, F. P. and YANG, C. S., 1988, Metabolism of N-nitrosodialkylamines by human liver microsomes. Cancer Research, 48, 1499–1504.

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