Phase I and II enzyme characterization of two sources of HepG2 cell lines

Reference

• ALLEN, S. W., MUELLER, L., WILLIAMS, S. N., QUATTROCHI, L. C. and RAUCY, J., 2001, The Use Of a high-volume screening procedure to assess the effects of dietary fiavonoids on human cyplal expression. Drug Metabolism and Disposition, 29, 1074–1079.
   PubMed Web of Science ®Google Scholar
• ARLOTTO, M. P., TRANT, J. M. and ESTABROOK, R. W., 1991, Measurement of steroid hydroxylation reactions by high-performance liquid chromatography as indicator of P450 identity and function. Methods in Enzymology, 206, 454–462.
   PubMed Web of Science ®Google Scholar
• CAMPBELL, N. R. C., VAN LOON, J. A. and WEISHILBOUM, R. M., 1987, Human liver phenol sulfotransferase: assay conditions, biochemical properties and partial purification of isozymes of the thermostable form. Biochemical Pharmacology, 36, 1435–1446.
   Google Scholar
• CHEN, Z. R., SOMOGYI, A. A. and BOCHNER, F., 1990, Simultaneous determination of dextromethorphan and three metabolites in plasma and urine using high-performance liquid chromatography with application to their disposition in man. Therapeutic Drug Monitoring, 12, 97–104.
   PubMed Web of Science ®Google Scholar
• CHiu, S.-H. L. and HUSKEY, S.-E. W., 1998, Species differences in N-glucuronidation. Drug Metabolism and Disposition, 26, 838–847.
   PubMed Web of Science ®Google Scholar
• DIERICKX, P. J., 1987, Inhibition of glutathione-dependent uridine uptake in cultured human hepatoma cells. Medical Science Research, 15, 1349–1350.
   Google Scholar
• DONDI, D., MAGGI, R., SCACCIANOCE, E., MARTINI, L., MOTTA, M., POLETTI, A., 2001, Expression and role of functional glucocorticoid receptors in the human androgen-independent prostate cancer cell line, DU145. Journal of Molecular Endocrinology, 26, 185–191.
   PubMed Web of Science ®Google Scholar
• DVORAK, Z., MODRIANSKY, M., PICHARD-GARCIA, L., BALAGUER, P., VILAREM, M. J., ULRICHOVA, J., MAUREL, P. and PASCUSSI, J. M., 2003, Colchicine down-regulates cytochrome P450 2B6, 2C8, 2C9, and 3A4 in human hepatocytes by affecting their glucocorticoid receptor-mediated regulation. Molecular Pharmacology, 64, 160–169.
   Google Scholar
• EKINS, S., MURRAY, G. I., BURKE, B. M., WILLIAMS, J. A., MARCHANT, N. C. and HAWKINS, G. M., 1995, Quantitative differences in Phase I and II metabolism between rat precision-cut liver slices and isolated hepatocytes. Drug Metabolism and Disposition, 23, 1274–1279.
   PubMed Web of Science ®Google Scholar
• FABRE, G., RAHMANI, R., PLACIDI, M., COMBALBERT, J., Coo, J., CANO, J. P., COULANGE, C., DUCROS, M. and RAMPAL, M., 1988, Characterization of midazolam metabolism using human hepatic microsomal fractions and hepatocytes in suspension obtained by perfusing whole human livers. Biochemical Pharmacology, 37, 4389–4397.
   PubMed Web of Science ®Google Scholar
• FEIERMAN, D. E., MELNIKOV, Z. and ZHANG, J., 2002, The paradoxical effect of acetaminophen on CYP3A4 activity and content in transfected HepG2 cells. Archives of Biochemical Biophysics, 398, 109–117.
   PubMed Web of Science ®Google Scholar
• GERBAL-CHALOIN, S., PASCUSSI, J.-M., PICARD-GARCIA, L., DAUJAT, M., WAECHTER, F., FABRE, J.-M., CARRERE, N. and MAUREL, P., 2001, Induction of CYP2C genes in human hepatocytes in primary culture. Drug Metabolism and Disposition, 29, 242–251.
   Google Scholar
• GROSS-STEINMEYER, K., STAPLETON, P. L., TRACY, J. H., BAMMLER, T. K. and EATON, D. L., 2001, Proceedings of the 6th International ISSX Meeting, Munich.
   Google Scholar
• HABIG, W. H., PABST, M. J. and JAK0BY, W. B., 1974, Glutathione S-transferase: a novel kinetic mechanism in which the major reaction pathway depends on substrate concentration. Journal of Biological Chemistry, 249, 7130–7139.
   Google Scholar
• HENGSTLER, J. G., RINGEL, M., BIEFANG, K., HAMMEL, S., MILBERT, U., GERL, M., KLEBACH, M., DIENER, B., PLATT, K. L., BOTTGER, T., STEINBERG, P. and OESCH, F., 2000, Cultures with cryopreserved hepatocytes: applicability for studies of enzyme induction. Chemical and Biological Interactions, 125, 51–73.
   PubMed Web of Science ®Google Scholar
• HEWITT, N. J, BUHRING, K. U., DASENBROCK, J., HAUNSCHILD, J., LADSTETTER, B. and UTESCH, D. 2001, Studies comparing in vivo:in vitro metabolism of three pharmaceutical compounds in rat, dog, monkey, and human using cryopreserved hepatocytes, microsomes, and collagen gel immobilized hepatocyte cultures. Drug Metabolism and Disposition, 29, 1042–1050.
   PubMed Web of Science ®Google Scholar
• HICKMAN, D., WANG, J.-P., WANG, Y. and UNADKAT, J. D., 1998, Evaluation of the selectivity of in vitro probes and suitability of organic solvents for the measurement of human cytochrome P450 monooxygenase activities. Drug Metabolism and Disposition, 26, 207–215.
   Google Scholar
• KANE, R. E., LI, A. P. and KAMINSKI, D. R., 1995, Sulfation and glucuronidation of acetaminophen by human hepatocytes cultured on Matrigel and type 1 collagen reproduces conjugation in vivo. Drug Metabolism and Disposition, 23, 303–307.
   PubMed Web of Science ®Google Scholar
• KERN, A., BADER, A., PICHLMAY R. and SEWING, K -F., 1997, Drug metabolism in hepatocyte sandwich cultures of rats and humans. Biochemical Pharmacology, 54,761–772.
   Google Scholar
• LEDIRAC, N., DE SOUSA, G., FONTANE, F., AGOURIDAS, C., GUGENHEIM, J., LORENZON, G. and RAHMANI, R., 2000, Effects of macrolide antibiotics on CYP3A expression in human and rat hepatocytes: interspecies differences in response to troleandomycin. Drug Metabolism and Disposition, 28, 1391–1393.
   PubMed Web of Science ®Google Scholar
• LI, A. P., GORYCKI, P. D., HENGSTLER, J. G., KEDDERIS, G. L., KOEBE, H. G., RAHMANI, R., DE SOUSAS, G., SILVA, J. M. and SKETT, P., 1999b, Present status of the application of cryopreserved hepatocytes in the evaluation of xenobiotics: consensus of an international expert panel. Chemical and Biological Interactions, 121, 117–123.
   PubMed Web of Science ®Google Scholar
• LI, A. P., Lu, C., BRENT, J. A., PHAM, C., FACKETT, A., RUEGG, C. E. and SILBER, P. M., 1999a, Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug-drug interaction potential. Chemical and Biological Interactions, 121, 17–35.
   PubMed Web of Science ®Google Scholar
• LUBET, R. A., Nims, R. W., MAYER, R. T., CAMERON, J. W. and SCHECHTMAN, L. M., 1985, Measurement of cytochrome P-450 dependent dealkylation of alkoxyphenoxazones in hepatic S9s and hepatocyte homogenates: effects of dicumarol. Mutation Research, 142, 127–131.
   PubMed Web of Science ®Google Scholar
• MARSELOS, M., STORM, S. C. and MICHALOPOULOS, G., 1987, Effect of phenobarbital and 3-methylcholanthrene on aldehyde dehydrogenase activity in cultures of HepG2 cells and normal human hepatocytes. Chemico-Biological Interactions, 62, 75–88.
   PubMed Web of Science ®Google Scholar
• MEUNIER, V., BOURRIE, M., JULIAN, B., MARTI, E., GUILOU, F., BERGER, Y and FABRE, G., 2000, Expression and induction of CYP1A1/1A2, CYP2A6 and CYP3A4 in primary cultures of human hepatocytes: a 10-year follow-up. Xenobiotica, 30, 589–607.
   Google Scholar
• MINERS, J. 0., SMITH, K. J., ROBSON, R. A., McMANus, M. E., VERONESE, M. E. and BIRKETT, D. J., 1988, Tolbutamide hydroxylation by human liver microsomes. Kinetic characterisation and relationship to other cytochrome P-450 dependent xenobiotic oxidations. Biochemical Pharmacology, 37, 1137–1144.
   Google Scholar
• O'LEARY, K. A., DAY, A. J., PAUL W. NEEDS, P. W., MELLON, F. A., O'BRIEN, N. M. and WILLIAMSON, G., 2003, Metabolism of quercetin-7- and quercetin-3-glucuronides by an in vitro hepatic model: the role of human 13-glucuronidase, sulfotransferase, catechol-O-methyltransferase and multi-resistant protein 2 (MRP2) in flavonoid metabolism. Biochemical Pharmacology, 65, 479–491.
   Google Scholar
• PARKER, R. S., SONTAG, T. J. and SWANSON, J. E., 2000, Cytochrome P4503A-dependent metabolism of tocopherols and inhibition by sesamin. Biochemical and Biophysical Research Communications, 277, 531–534.
   PubMed Web of Science ®Google Scholar
• PASCUSSI, J. M., JOUNAIDI, Y., DROCOURT, L., DOMERGUE, J., BALABAUD, C., MAUREL, P. and VILAREM, M. J., 1999, Evidence for the presence of a functional pregnane X receptor response element in the CYP3A7 promoter gene. Biochemical and Biophysical Research Communications, 260, 377–381.
   PubMed Web of Science ®Google Scholar
• PASCUSSI, J. M., GERBAL-CHALOIN, S., FABRE, J. M., MAUREL, P. and VILAREM, M. J., 2000a, DEX enhances constitutive androstane receptor expression in human hepatocytes: consequences on cytochrome P450 gene regulation. Molecular Pharmacology, 58, 1441–1450.
   Google Scholar
• PASCUSSI, J. M., GERBAL-CHALOIN, S., PICHARD-kGARCIA, L., DAUJAT, M., FABRE, J. M., MAUREL, P. and VILAREM, M. J., 2000b, Interleukin-6 negatively regulates the expression of pregnane X receptor and constitutively activated receptor in primary human hepatocytes. Biochemical and Biophysical Research Communications, 274, 707–713.
   Google Scholar
• REITER, C. and WEINSHILBOUM, R. M., 1982, Acetaminophen and phenol: substrates for both a thermostable and a thermolabile form of human platelet phenol sulfotransferase. Journal of Pharmacology and Experimental Therapeutics, 231, 43–51.
   Google Scholar
• SASSA, S., SUGITA, 0., GALBRAITH, R. A. and KAPPAS, A., 1987, Drug metabolism by the human hepatoma cell, HepG2. Biochemical and Biophysical Research Communications, 143, 52–57.
   Google Scholar
• SCHEERS, E. M., EKWALL, B. and DIERICKX, P. J., 2001, In vitro long-term cytotoxicity testing of 27 MEIC chemicals on HepG2 cells and comparison with acute human toxicity data. Toxicology in Vitro, 15, 153–161.
   PubMed Web of Science ®Google Scholar
• SCHUETZ, E. G., SCHUETZ, J. D., STROM, S. C., THOMPSON, M. T., FISHER, R. A., MOLOWA, D. T., LI, D. and GUZELIAN, P. S., 1993, Regulation of human liver cytochromes P-450 in family 3A in primary and continuous culture of human hepatocytes. Hepatology, 18, 1254–1262.
   PubMed Web of Science ®Google Scholar
• STEVENS, H. M. and GILL, R., 1986, High-performance liquid chromatography systems for the analysis of analgesic and non-steroidal anti-inflammatory drugs in forensic toxicology. Journal of Chromatography, 370, 39–47.
   PubMed Web of Science ®Google Scholar
• TODD, M. D., LEE, M. J., WILLIAMS, J. L., NALEZNY, J. M., GEE, P., BENJAMIN, M. B. and FARR, S. B., 1995, The CAT-Tox (L) assay: a sensitive and specific measure of stress-induced transcription in transformed human liver cells. Fundamental and Applied Toxicology, 28, 118–128.
   PubMedGoogle Scholar
• VERONESE, M. E. and MCCLEAN, S., 1991, Debrisoquine oxidation polymorphism in a Tasmanian population. European Journal of Clinical Pharmacology, 40, 547–452.
   PubMed Web of Science ®Google Scholar
• WALLE, T., OTAKE, Y., GALIJATOVIC, A., RITTER, J. K. and WALLE, U. K., 2000, Induction of UDP-glucuronosyltransferase UGT1A1 by the fiavonoid chrysin in the human hepatoma cell line HepG2. Drug Metabolism and Disposition, 28, 1077–1082.
   PubMed Web of Science ®Google Scholar
• WITHEROW, L. E. and HOUSTON, J. B., 1999, Sigmoidal kinetics of CYP3A substrates: an approach for scaling dextromethorphan metabolism in hepatic microsomes and isolated hepatocytes to predict in vivo clearance in rat. Pharmacology and Experimental Therapeutics, 290, 58–65.
   PubMed Web of Science ®Google Scholar
• YOSHITOMI, S., IKEMOTO, K., TAKAHASHI, J., MIKI, H., NAMBA, M. and ASAHI, S., 2001, Establishment of the transformants expressing human cytochrome P450 subtypes in HepG2, and their applications on drug metabolism and toxicology. Toxicology in Vitro, 15, 245–256.
   PubMed Web of Science ®Google Scholar
• YUSUF, A. T., VIAN, L., SABATIER, R. and CANO, J. P., 2000, In vitro detection of indirect-acting genotoxins in the comet assay using HepG2 cells. Mutation Research, 468, 227–234.
   PubMed Web of Science ®Google Scholar