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Xenobiotica
the fate of foreign compounds in biological systems
Volume 48, 2018 - Issue 12
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General Xenobiochemistry

Qualitative and quantitative prediction of human in vivo metabolic pathways in a human hepatocyte-murine stromal cell co-culture model

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Pages 1192-1205 | Received 22 Sep 2017, Accepted 19 Oct 2017, Published online: 16 Nov 2017

References

  • Ballard TE, Wang S, Cox LM, et al. (2016). Application of a micropatterned cocultured hepatocyte system to predict preclinical and human-specific drug metabolism. Drug Metabol Dispos 44:172–9
  • Bonn B, Svanberg P, Janefeldt A, et al. (2016). Determination of human hepatocyte intrinsic clearance for slowly metabolized compounds: comparison of a primary hepatocyte/stromal cell co-culture with plated primary hepatocytes and HepaRG. Drug Metab Dispos 44:527–33
  • Brown HS, Griffin M, Houston JB. (2007). Evaluation of cryopreserved human hepatocytes as an alternative in vitro system to microsomes for the prediction of metabolic clearance. Drug Metab Dispos 35:293–301
  • Chan TS, Yu H, Moore A, et al. (2013). Meeting the challenge of predicting hepatic clearance of compounds slowly metabolized by cytochrome P450 using a novel hepatocyte model, HepatoPac. Drug Metab Dispos 41:2024–32
  • Cho C, Pludwinski E, Parekh A, et al. HμRELhumanPool™: combining the phenotypic breadth of pooled primary hepatocytes with the stable, long-enduring cellular competency of HμREL® co-culture technology; 2014. ISSX
  • Darnell M, Ulvestad M, Ellis E, et al. (2012). In vitro evaluation of major in vivo drug metabolic pathways using primary human hepatocytes and HepaRG cells in suspension and a dynamic three-dimensional bioreactor system. J Pharmacol Exp Ther 343:134–44
  • Di L, Obach RS. (2015). Addressing the challenges of low clearance in drug research. AAPS J 17:352–7
  • Grubb M, Shu Y-Z, Humphreys WG. Comparison of long-term hepatocyte cultures for determining the metabolic profiles of metabolically stable drug candidates; 2015. ISSX, Poster 194
  • Hultman I, Vedin C, Abrahamsson A, et al. (2016). Use of HμREL human coculture system for prediction of intrinsic clearance and metabolite formation for slowly metabolized compounds. Mol Pharm 13:2796–807
  • Hutzler JM, Ring BJ, Anderson SR. (2015). Low-turnover drug molecules: a current challenge for drug metabolism scientists. Drug Metab Dispos 43:1917–28
  • Li AP, Lu C, Brent JA, et al. (1999). Cryopreserved human hepatocytes: characterization of drug-metabolizing enzyme activities and applications in higher throughput screening assays for hepatotoxicity, metabolic stability, and drug–drug interaction potential. Chem-Biol Interact 121:17–35
  • Novik E, Maguire TJ, Chao P, et al. (2010). A microfluidic hepatic coculture platform for cell-based drug metabolism studies. Biochem Pharmacol 79:1036–44
  • Ohkura T, Ohta K, Nagao T, et al. (2014). Evaluation of human hepatocytes cultured by three-dimensional spheroid systems for drug metabolism. Drug Metab Pharmacokinet 29:373–8
  • Rodriguez-Antona C, Donato MT, Boobis A, et al. (2002). Cytochrome P450 expression in human hepatocytes and hepatoma cell lines: molecular mechanisms that determine lower expression in cultured cells. Xenobiotica 32:505–20
  • Roffel AF, van Marle SP, van Lier JJ, et al. (2016). An evaluation of human ADME and mass balance studies using regular or low doses of radiocarbon. J Label Compd Radiopharm 59:619–26
  • Smith CM, Nolan CK, Edwards MA, et al. (2012). A comprehensive evaluation of metabolic activity and intrinsic clearance in suspensions and monolayer cultures of cryopreserved primary human hepatocytes. J Pharm Sci 101:3989–4002
  • Wang WW, Khetani SR, Krzyzewski S, et al. (2010). Assessment of a micropatterned hepatocyte coculture system to generate major human excretory and circulating drug metabolites. Drug Metabol Dispos 38:1900–5

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