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Xenobiotica
the fate of foreign compounds in biological systems
Volume 50, 2020 - Issue 6
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General Xenobiochemistry

Hepatocyte spheroids as a viable in vitro model for recapitulation of complex in vivo metabolism pathways of loratadine in humans

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Pages 621-629 | Received 01 Jul 2019, Accepted 28 Sep 2019, Published online: 10 Oct 2019

References

  • Abu-Absi S, Hansen LK, Wei-Shou H. (2004). Three-dimensional co-culture of hepatocytes and stellate cells. Cytotechnology 45:125–40.
  • Aratyn-Schaus Y, Ramanathan R. (2016). Advances in high-resolution MS and hepatocyte models solve a long-standing metabolism challenge: the loratadine story. Bioanalysis 8:1645–62.
  • Bell C, Hendriks D, Moro S, et al. (2016). Characterization of primary human hepatocyte spheroids as a model system for drug-induced liver injury, liver function and disease. Sci Rep 6:1–13.
  • Ghosal A, Gupta S, Ramanathan R, et al. (2009). Metabolism of loratadine and further characterization of its in vitro metabolites. Drug Metab Lett 3:162–70.
  • Hendricks D, Puigvert L, Messner S, et al. (2016). Hepatic 3D spheroid models for detection and study of compounds with cholestatic liability. Nature Sci Rep 6:1–11.
  • Kazmi F, Barbara J, Yerino P, Parkinson A. (2015). A long-standing mystery solved: the formation of 3-hydroxydesloratadine is catalyzed by CYP2C8 but prior glucuronidation of desloratadine by UDP-glucuronosyltransferase 2B10 is an obligatory requirement. Drug Metab Dispos 43:523–33.
  • Kazmi F, Yerino P, Barbara J, Parkinson A. (2015). Further characterization of the metabolism of desloratadine and its cytochrome P450 and UDP-glucuronosyltransferase inhibition potential: identification of desloratadine as a relatively selective UGT2B10 inhibitor. Drug Metab Dispos 43:1294–302.
  • Lauschke VM, Hendriks DF, Bell CC, et al. (2016). Novel 3D culture systems for studies of human liver function and assessments of the hepatotoxicity of drugs and drug candidates. Chem Res Toxicol 29:1936–55.
  • Lu H, Chua K, Zhang P, et al. (2005). Three-dimensional co-culture of rat hepatocyte spheroids and NIH/3T3 fibroblasts enhances hepatocyte functional maintenance. Acta Biomater 4:399–410.
  • Ohkura T, Ohta K, Nagao T, et al. (2014). Evaluation of human hepatocytes cultured by three-dimensional spheroid systems for drug metabolism. Drug Metab Pharmacokinet 29:373–8.
  • Pekthong D, Desbans C, Martin H, Richert L. (2012). Bupropion hydroxylation as a selective marker of rat CYP2B1 catalytic activity. Drug Metab Dispos 40:32–8.
  • Pinheiro PF, Pereira SA, Harjivan SG, et al. (2017). Hepatocyte spheroids as a competent in vitro system for drug biotransformation studies: nevirapine as a bioactivation case study. Arch Toxicol 91:1199–211.
  • Ramanathan R, Alvarez N, Su A, et al. (2005). Metabolism and excretion of loratadine in male and female mice, rats and monkeys. Xenobiotica 35:155–89.
  • Ramanathan R, Reyderman L, Kulmatycki K, et al. (2007). Disposition of loratadine in healthy volunteers. Xenobiotica 37:753–69.
  • Ukairo O, Kanchagar C, Moore A, et al. (2013). Long-term stability of primary rat hepatocytes in micropatterned cocultures. J Biochem Mol Toxicol 27:204–12.
  • Vorrink SU, Ullah S, Schmidt S, et al. (2017). Endogenous and xenobiotic metabolic stability of primary human hepatocytes in long-term 3D spheroid cultures revealed by a combination of targeted and untargeted metabolomics. Faseb J 31:2696–708.
  • White RW. 2014. The importance of full and correct prediction of human metabolites in discovery and preclinical development. A Hµrel White paper. California: Hµrel Corporation.

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