A high-throughput cell-based gaussia luciferase reporter assay for measurement of CYP1A1, CYP2B6, and CYP3A4 induction

References

• Back, D.J., 2006. Drug-drug interactions that matter. Top HIV medications, 14 (2), 88–92.
   PubMedGoogle Scholar
• Bartonkova, I., and Dvorak, Z., 2018. Essential oils of culinary herbs and spices activate PXR and induce CYP3A4 in human intestinal and hepatic in vitro models. Toxicology letters, 296, 1–9.
   PubMed Web of Science ®Google Scholar
• Bertilsson, G., et al., 1998. Identification of a human nuclear receptor defines a new signaling pathway for CYP3A induction. Proceedings of the National Academy of Sciences, 95 (21), 12208–12213.
   PubMed Web of Science ®Google Scholar
• Burke, M.D., et al., 1994. Cytochrome P450 specificities of alkoxyresorufin O-dealkylation in human and rat liver. Biochemical pharmacology, 48 (5), 923–936.
   PubMed Web of Science ®Google Scholar
• Büssow, K., 2015. Stable mammalian producer cell lines for structural biology. Current opinion in structural biology., 32, 81–90.
   PubMed Web of Science ®Google Scholar
• Cali, J.J., et al., 2006. Luminogenic cytochrome P450 assays. Expert opinion on drug metabolism & toxicology, 2 (4), 629–645.
   PubMed Web of Science ®Google Scholar
• Chacko, S.A., et al., 2020. Hepatocyte spheroids as a viable in vitro model for recapitulation of complex in vivo metabolism pathways of loratadine in humans. Xenobiotica, 50 (6), 621–629.
   PubMed Web of Science ®Google Scholar
• Chen, M., et al., 2020. Identification of rhythmic human CYPs and their circadian regulators using synchronized hepatoma cells. Xenobiotica, 50 (9), 1052–1063.
   PubMed Web of Science ®Google Scholar
• Chen, S., et al., 2021. Characterization of cytochrome P450s (CYP)-overexpressing HepG2 cells for assessing drug and chemical-induced liver toxicity. Journal of environmental science health, part C: Toxicology and Carcinogenesis, 39 (1), 68–86.
   PubMed Web of Science ®Google Scholar
• Choi, J.M., et al., 2015. HepG2 cells as an in vitro model for evaluation of cytochrome P450 induction by xenobiotics. Archives of pharmacal research, 38 (5), 691–704.
   PubMed Web of Science ®Google Scholar
• Cui, X., et al., 2002. A high-throughput cell-based reporter gene system for measurement of CYP1A1 induction. Journal of pharmacological and toxicological methods, 47 (3), 143–151.
   PubMed Web of Science ®Google Scholar
• DeGroot, D.E., et al., 2015. Naturally occurring marine brominated indoles are aryl hydrocarbon receptor ligands/agonists. Chemical research in toxicology, 28 (6), 1176–1185.
   PubMed Web of Science ®Google Scholar
• DeGroot, D.E., et al., 2018. mRNA transfection retrofits cell-based assays with xenobiotic metabolism. Journal of pharmacological and toxicological methods, 92, 77–94.
   PubMed Web of Science ®Google Scholar
• Di Masi, A., et al., 2009. Nuclear receptors CAR and PXR: Molecular, functional, and biomedical aspects. Molecular aspects of medicine, 30 (5), 297–343.
   PubMed Web of Science ®Google Scholar
• Donato, M.T., et al., 2008. Cell lines: a tool for in vitro drug metabolism studies. Current drug metabolism, 9 (1), 1–11.
   PubMed Web of Science ®Google Scholar
• Fery, Y., Mueller, S.O., and Schrenk, D., 2010. Development of stably transfected human and rat hepatoma cell lines for the species-specific assessment of xenobiotic response enhancer module (XREM)-dependent induction of drug metabolism. Toxicology, 277 (1–3), 11–19.
   PubMed Web of Science ®Google Scholar
• Gómez-Lechón, M.J., et al., 2014. Competency of different cell models to predict human hepatotoxic drugs. Expert opinion on drug metabolism & toxicology., 10 (11), 1553–1568.
   PubMed Web of Science ®Google Scholar
• Guengerich, F.P., 1999. Cytochrome P-450 3A4: regulation and role in drug metabolism. Annual review of pharmacology and toxicology, 39 (1), 1–17.
   PubMed Web of Science ®Google Scholar
• Guengerich, F.P., 2008. Cytochrome p450 and chemical toxicology. Chemical research in toxicology, 21 (1), 70–83.
   PubMed Web of Science ®Google Scholar
• Hedrich, W.D., Hassan, H.E., and Wang, H., 2016. Insights into CYP2B6-mediated drug-drug interactions. Acta pharmaceutica sinica B, 6 (5), 413–425.
   PubMed Web of Science ®Google Scholar
• Hornberg, J.J., et al., 2014. Exploratory toxicology as an integrated part of drug discovery. Part I: why and how. Drug discovery today, 19 (8), 1131–1136
   PubMed Web of Science ®Google Scholar
• Kanno, Y., and Inouye, Y., 2010. A consecutive three alanine residue insertion mutant of human CAR: a novel CAR ligand screening system in HepG2 cells. The journal of toxicological sciences, 35 (4), 515–525.
   PubMed Web of Science ®Google Scholar
• Kim, R.B., 2002. Transporters and xenobiotic disposition. Toxicology, 181–182, 291–297.
   PubMed Web of Science ®Google Scholar
• Kola, I., and Landis, J., 2004. Can the pharmaceutical industry reduce attrition rates? Nature reviews drug discovery, 3 (8), 711–715.
   PubMed Web of Science ®Google Scholar
• Kress, S., Reichert, J., and Schwarz, M., 1998. Functional analysis of the human cytochrome P4501A1 (CYP1A1) gene enhancer. European journal of biochemistry, 258 (2), 803–812.
   PubMedGoogle Scholar
• Kumagai, T., et al., 2016. Indirubin, a component of Ban-Lan-Gen, activates CYP3A4 gene transcription through the human pregnane X receptor. Drug metabolism and pharmacokinetics, 31 (2), 139–145.
   PubMed Web of Science ®Google Scholar
• Lehmann, J.M., et al., 1998. The human orphan nuclear receptor PXR is activated by compounds that regulate CYP3A4 gene expression and cause drug interactions. Journal of clinical investigation, 102 (5), 1016–1023.
   PubMed Web of Science ®Google Scholar
• Li, H., et al., 2017. In vivo cytochrome P450 activity alterations in diabetic nonalcoholic steatohepatitis mice. Journal of biochemical and molecular toxicology, 31 (2), e21840.
   Web of Science ®Google Scholar
• Liao, M., et al., 2020. Evaluation of in vitro absorption, distribution, metabolism, and excretion and assessment of drug-drug interaction of rucaparib, an orally potent poly (ADP-ribose) polymerase inhibitor. Xenobiotica, 50 (9), 1032–1042.
   PubMed Web of Science ®Google Scholar
• Lněničková, K., et al., 2018. The impact of sesquiterpenes β-caryophyllene oxide and trans-nerolidol on xenobiotic-metabolizing enzymes in mice in vivo. Xenobiotica, 48 (11), 1089–1097.
   PubMed Web of Science ®Google Scholar
• Luckert, C., et al., 2013. Polycyclic aromatic hydrocarbons stimulate human CYP3A4 promoter activity via PXR. Toxicology letters, 222 (2), 180–188.
   PubMed Web of Science ®Google Scholar
• Maglich, J.M., et al., 2002. Nuclear pregnane x receptor and constitutive androstane receptor regulate overlapping but distinct sets of genes involved in xenobiotic detoxification. Molecular pharmacology, 62 (3), 638–646.
   PubMed Web of Science ®Google Scholar
• Meredith, C., et al., 2003. Studies on the induction of rat hepatic CYP1A, CYP2B, CYP3A and CYP4A subfamily form mRNAs in vivo and in vitro using precision-cut rat liver slices. Xenobiotica, 33 (5), 511–527.
   PubMed Web of Science ®Google Scholar
• Michelini, E., et al., 2009. Luminescent probes and visualization of bioluminescence. Methods in molecular biology, 574, 1–13.
   PubMedGoogle Scholar
• Moore, J.T., and Kliewer, S.A., 2000. Use of the nuclear receptor PXR to predict drug interactions. Toxicology, 153 (1–3), 1–10.
   PubMed Web of Science ®Google Scholar
• Nannelli, A., et al., 2008. Expression and induction by rifampicin of CAR- and PXR-regulated CYP2B and CYP3A in liver, kidney and airways of pig. Toxicology, 252 (1–3), 105–112.
   PubMed Web of Science ®Google Scholar
• Nedelcheva, V., and Gut, I., 1994. P450 in the rat and man: methods of investigation, substrate specificities and relevance to cancer. Xenobiotica, 24 (12), 1151–1175.
   PubMed Web of Science ®Google Scholar
• Noracharttiyapot, W., et al., 2006. Construction of several human-derived stable cell lines displaying distinct profiles of CYP3A4 induction. Drug metabolism and pharmacokinetics, 21 (2), 99–108.
   PubMed Web of Science ®Google Scholar
• Ortega Ugalde, S., et al., 2019. Evaluation of luminogenic substrates as probe substrates for bacterial cytochrome P450 enzymes: application to Mycobacterium tuberculosis. SLAS discovery: advancing the science of drug discovery, 24 (7), 745–754.
   Google Scholar
• Pascussi, J.M., et al., 2001. Dual effect of dexamethasone on CYP3A4 gene expression in human hepatocytes. Sequential role of glucocorticoid receptor and pregnane X receptor. European journal of biochemistry, 268 (24), 6346–6358.
   PubMedGoogle Scholar
• Reyes, H., Reisz-Porszasz, S., and Hankinson, O., 1992. Identification of the Ah receptor nuclear translocator protein (Arnt) as a component of the DNA binding form of the Ah receptor. Science, 256 (5060), 1193–1195.
   PubMed Web of Science ®Google Scholar
• Rhodes, S.P., et al., 2011. Simultaneous assessment of cytochrome P450 activity in cultured human hepatocytes for compound-mediated induction of CYP3A4, CYP2B6, and CYP1A2. Journal of pharmacological and toxicological methods, 63 (3), 223–226.
   PubMed Web of Science ®Google Scholar
• Satoh, D., et al., 2017. Establishment of a novel hepatocyte model that expresses four cytochrome P450 genes stably via mammalian-derived artificial chromosome for pharmacokinetics and toxicity studies. PLoS One, 12 (10), e0187072.
   PubMed Web of Science ®Google Scholar
• Sekimoto, M., et al., 2012. Establishment of a stable human cell line, HPL-A3, for use in reporter gene assays of cytochrome P450 3A inducers. Biological and pharmaceutical bulletin, 35 (5), 677–685.
   PubMed Web of Science ®Google Scholar
• Sérée, E., et al., 2004. Evidence for a new human CYP1A1 regulation pathway involving PPAR-alpha and 2 PPRE sites. Gastroenterology, 127 (5), 1436–1445.
   PubMed Web of Science ®Google Scholar
• Shimada, T., et al., 1994. Interindividual variations in human liver cytochrome P-450 enzymes involved in the oxidation of drugs, carcinogens and toxic chemicals: studies with liver microsomes of 30 Japanese and 30 Caucasians. Journal of pharmacology and experimental therapeutics., 270 (1), 414–423.
   PubMed Web of Science ®Google Scholar
• Soars, M.G., et al., 2007. The pivotal role of hepatocytes in drug discovery. Chemico-biological interactions, 168 (1), 2–15.
   PubMed Web of Science ®Google Scholar
• Song, Y., et al., 2021. Drug-metabolizing cytochrome p450 enzymes have multifarious influences on treatment outcomes. Clinical pharmacokinetics.
   PubMed Web of Science ®Google Scholar
• Stevison, F., et al., 2019. Does in vitro cytochrome P450 downregulation translate to in vivo drug-drug interactions? Preclinical and clinical studies with 13-cis-retinoic acid. Clinical and translational science, 12 (4), 350–360.
   PubMed Web of Science ®Google Scholar
• Sueyoshi, T., and Negishi, M., 2001. Phenobarbital response elements of cytochrome P450 genes and nuclear receptors. Annual review of pharmacology and toxicology, 41 (1), 123–143.
   PubMedGoogle Scholar
• Sumida, A., et al., 2000. Quantitative analysis of constitutive and inducible CYPs mRNA expression in the HepG2 cell line using reverse transcription-competitive PCR. Biochemical and biophysical research communications, 267 (3), 756–760.
   PubMed Web of Science ®Google Scholar
• Swales, K., et al., 2005. Novel CAR-mediated mechanism for synergistic activation of two distinct elements within the human cytochrome P450 2B6 gene in HepG2 cells. Journal of biological chemistry, 280 (5), 3458–3466.
   PubMed Web of Science ®Google Scholar
• Tannous, B.A., et al., 2005. Codon-optimized Gaussia luciferase cDNA for mammalian gene expression in culture and in vivo. Molecular therapy, 11 (3), 435–443.
   PubMed Web of Science ®Google Scholar
• Tolosa, L., et al., 2012. Upgrading cytochrome P450 activity in HepG2 cells co-transfected with adenoviral vectors for drug hepatotoxicity assessment. Toxicology in vitro, 26 (8), 1272–1277.
   PubMed Web of Science ®Google Scholar
• Tsuji, S., et al., 2016. A cytoplasmic form of gaussia luciferase provides a highly sensitive test for cytotoxicity. PLOS One, 11 (5), e0156202.
   PubMed Web of Science ®Google Scholar
• Underhill, G.H., and Khetani, S.R., 2018. Advances in engineered human liver platforms for drug metabolism studies. Drug metabolism and disposition, 46 (11), 1626–1637.
   PubMed Web of Science ®Google Scholar
• Urquhart, B.L., Tirona, R.G., and Kim, R.B., 2007. Nuclear receptors and the regulation of drug-metabolizing enzymes and drug transporters: implications for interindividual variability in response to drugs. The journal of clinical pharmacology, 47 (5), 566–578.
   PubMed Web of Science ®Google Scholar
• Vrzal, R., et al., 2009. Dexamethasone controls aryl hydrocarbon receptor (AhR)-mediated CYP1A1 and CYP1A2 expression and activity in primary cultures of human hepatocytes. Chemico-biological interactions., 179 (2–3), 288–296.
   PubMed Web of Science ®Google Scholar
• Wohak, L.E., et al., 2016. Carcinogenic polycyclic aromatic hydrocarbons induce CYP1A1 in human cells via a p53-dependent mechanism. Archives of toxicology, 90 (2), 291–304.
   PubMed Web of Science ®Google Scholar
• Xu, C., Li, C.Y., and Kong, A.N., 2005. Induction of phase I, II and III drug metabolism/transport by xenobiotics. Archives of pharmacal research, 28 (3), 249–268.
   PubMed Web of Science ®Google Scholar
• Yang, S.K., 1988. Stereoselectivity of cytochrome P-450 enzymes and epoxide hydrolase in the metabolism of polycyclic aromatic hydrocarbons. Biochemical pharmacology, 37 (1), 61–70.
   PubMed Web of Science ®Google Scholar
• Yokoyama, Y., et al., 2018. Comparison of drug metabolism and its related hepatotoxic effects in HepaRG, cryopreserved human hepatocytes, and HepG2 cell cultures. Biological and pharmaceutical bulletin, 41 (5), 722–732.
   PubMed Web of Science ®Google Scholar
• You, W.T., et al., 2016. Ophiopogonin D maintains Ca2+ homeostasis in rat cardiomyocytes in vitro by upregulating CYP2J3/EETs and suppressing ER stress. Acta pharmacologica sinica, 37 (3), 368–381.
   PubMed Web of Science ®Google Scholar
• Yu, Z.W., Lou, G.D., and Ge, L.L., 2021. Rapid identification of cytochrome P450 inductive constituents of Shenmai injection by combined pregnane X receptor reporter gene assay and LC-TOF-MS analysis. Journal of ethnopharmacology, 268, 113588.
   PubMed Web of Science ®Google Scholar
• Yueh, M.F., Kawahara, M., and Raucy, J., 2005. Cell-based high-throughput bioassays to assess induction and inhibition of CYP1A enzymes. Toxicology in vitro, 19 (2), 275–287.
   PubMed Web of Science ®Google Scholar
• Zanger, U.M., and Schwab, M., 2013. Cytochrome P450 enzymes in drug metabolism: regulation of gene expression, enzyme activities, and impact of genetic variation. Pharmacology & therapeutics., 138 (1), 103–141.
   PubMed Web of Science ®Google Scholar
• Zhang, X.Q., et al., 2020. Xiaoaiping injection enhances paclitaxel efficacy in ovarian cancer via pregnane X receptor and its downstream molecules. Journal of ethnopharmacology, 261, 113067.
   PubMed Web of Science ®Google Scholar