Genotoxicity of nicotine in cell culture of Caenorhabditis elegans evaluated by the comet assay

References

• Arabi, M. (2004). Nicotinic infertility: assessing DNA and plasma membrane integrity of human spermatozoa. Andrologia 36:305–310.
   PubMed Web of Science ®Google Scholar
• Argentin, G., Cicchetti, R. (2004). Genotoxic and antiapoptotic effect of nicotine on human gingival fibroblasts. Toxicol Sci 79:75–81.
   PubMed Web of Science ®Google Scholar
• Ashby, J., Tinwell, H., et al. (1995). The single-cell gel electrophoresis assay for induced DNA damage (comet assay): measurement of tail length and moment. Mutagenesis 10:85–90.
   PubMed Web of Science ®Google Scholar
• Bianchi, L., Driscoll, M. (2006). Culture of embryonic C. elegans cells for electrophysiological and pharmacological analyses (September 30, 2006), WormBook, ed. The C. elegans Research Community, doi/10.1895/wormbook.1.122.1, http://www.wormbook.org.
   Google Scholar
• Conte, C., Mutti, I., et al. (1998). DNA fingerprinting analysis by a PCR-based method for monitoring the genotoxic effects of heavy metals pollution. Chemosphere 37(14–15):2739–2749.
   PubMed Web of Science ®Google Scholar
• Crowley-Weber, C.L., Dvorakova, K., et al. (2003). Nicotine increases oxidative stress, activates NF-kappaB and GRP78, induces apoptosis, and sensitizes cells to genotoxic/xenobiotic stresses by a multiple stress inducer, deoxycholate: relevance to colon carcinogenesis. Chem Biol Interact 145:53–66.
   PubMed Web of Science ®Google Scholar
• Doolittle, D.J., Winegar, R., et al. (1995). The genotoxic potential of nicotine and its major metabolites. Mutat Res 344(3–4):95–102.
   PubMed Web of Science ®Google Scholar
• Guan, Z.Z., Yu, W.F., et al. (2003). Dual effects of nicotine on oxidative stress and neuroprotection in PC12 cells. Neurochem Int 43:243–249.
   PubMed Web of Science ®Google Scholar
• Gvozdjakova, A., Kucharska, J., et al. (1992). Effect of smoking on the oxidative processes of cardiomyocytes. Cardiology 81(2–3):81–84.
   PubMed Web of Science ®Google Scholar
• Hanes, P.J., Schuster, G.S., et al. (1991). Binding, uptake, and release of nicotine by human gingival fibroblasts. J Periodontol 62:147–152.
   PubMed Web of Science ®Google Scholar
• Hunt, L.A., Kelley, K.S. (1984). Nicotine-stimulated proteins in mouse cells are distinct from heat-shock proteins. Biochem J 224:87–92.
   PubMed Web of Science ®Google Scholar
• Ishidate, M., Jr, Miura, KF, et al. (1998). Chromosome aberration assays in genetic toxicology testing in vitro. Mutat Res 404(1–2):167–172.
   PubMed Web of Science ®Google Scholar
• Kleinsasser, N.H., Sassen, A.W., et al. (2005). The tobacco alkaloid, nicotine, demonstrates genotoxicity in human tonsillar tissue and lymphocytes. Toxicol Sci 86:309–317.
   PubMed Web of Science ®Google Scholar
• Konca, K., Lankoff, A., et al. (2003). A cross-platform public domain PC image-analysis program for the comet assay. Mutat Res 534(1–2):15–20.
   PubMed Web of Science ®Google Scholar
• Kovalchuk, O., Hendricks, C.A., et al. (2004). In vivo recombination after chronic damage exposure falls to below spontaneous levels in “recombomice.” Mol Cancer Res 2:567–573.
   PubMed Web of Science ®Google Scholar
• Linert, W., Bridge, M.H., et al. (1999). In vitro and in vivo studies investigating possible antioxidant actions of nicotine: relevance to Parkinson’s and Alzheimer’s diseases. Biochim Biophys Acta 1454:143–152.
   PubMed Web of Science ®Google Scholar
• Mizusaki, S., Okamoto, H., et al. (1977). Relation between chemical constituents of tobacco and mutagenic activity of cigarette smoke condensate. Mutat Res 48(3–4):319–325.
   PubMed Web of Science ®Google Scholar
• Nylen, E.S., Becker, K.L., et al. (1993). Cholinergic-nicotinic control of growth and secretion of cultured pulmonary neuroendocrine cells. Anat Rec 236:129–135.
   PubMedGoogle Scholar
• Obata, T., Aomine, M., et al. (2002). Nicotine suppresses 1-methyl- 4-phenylpyridinium ion-induced hydroxyl radical generation in rat striatum. Neurosci Lett 330:122–124.
   PubMed Web of Science ®Google Scholar
• Olive, P.L., Banath, J.P. (2006). The comet assay: a method to measure DNA damage in individual cells. Nat Prot 1:23–29.
   PubMed Web of Science ®Google Scholar
• Olive, P.L., Wlodek, D., et al. (1992). Factors influencing DNA migration from individual cells subjected to gel electrophoresis. Exp. Cell Res. 198:259–267.
   PubMed Web of Science ®Google Scholar
• Russell, M.A., Jarvis, M., et al. (1980). Relation of nicotine yield of cigarettes to blood nicotine concentrations in smokers. Br Med J 280:972–976.
   PubMed Web of Science ®Google Scholar
• Sassen, A.W., Richter, E., et al. (2005). Genotoxicity of nicotine in miniorgan cultures of human upper aerodigestive tract epithelia. Toxicol Sci 88:134–141.
   PubMed Web of Science ®Google Scholar
• Schuller, H.M. (1989). Cell-type-specific, receptor-mediated modulation of growth kinetics in human lung cancer cell lines by nicotine and tobacco-related nitrosamines. Biochem Pharmacol 38:3439–3442.
   PubMed Web of Science ®Google Scholar
• Shaham, S. (2006). Methods in cell biology. (January 02, 2006), WormBook, ed. The C. elegans Research Community, doi/10.1895/wormbook.1.49.1, http://www.wormbook.org.
   Google Scholar
• Soto-Otero, R., Mendez-Alvarez, E., et al. (2002). Effects of (-)- nicotine and (-)-cotinine on 6-hydroxydopamine-induced oxidative stress and neurotoxicity: relevance for Parkinson’s disease. Biochem Pharmacol 64:125–135.
   PubMed Web of Science ®Google Scholar
• Stiernagle, T. (2006). Maintenance of C. elegans. (February 11, 2006), WormBook, ed. The C. elegans Research Community, doi/10.1895/wormbook.1.101.1, http://www.wormbook.org.
   Google Scholar
• Strange, K., Christensen, M., et al. (2007). Primary culture of Caenorhabditis elegans developing embryo cells for electrophysiological, cell biological, and molecular studies. Nat Prot 2(4):1003–1012.
   PubMed Web of Science ®Google Scholar
• Strober, W. (2001). Trypan blue exclusion test of cell viability. Curr Protoc Immunol Appendix 3B.
   Google Scholar
• Trivedi, A.H., Dave, B.J., et al. (1990). Assessment of genotoxicity of nicotine employing in vitro mammalian test system. Cancer Lett 54(1–2):89–94.
   PubMed Web of Science ®Google Scholar
• Villablanca, A.C. (1998). Nicotine stimulates DNA synthesis and proliferation in vascular endothelial cells in vitro. J Appl Physiol 84:2089–2098.
   PubMed Web of Science ®Google Scholar
• Wetscher, G.J., Bagchi, M., et al. (1995). Free radical production in nicotine treated pancreatic tissue. Free Radic Biol Med 18:877–882.
   PubMed Web of Science ®Google Scholar
• Wu, H.J., Chi, C.W., et al. (2005). Effects of pH on nicotine-induced DNA damage and oxidative stress. J Toxicol Environ Health A 68(17–18):1511–1523.
   PubMed Web of Science ®Google Scholar
• Yildiz, D. (2004). Nicotine, its metabolism, and an overview of its biological effects. Toxicon 43:619–632.
   PubMed Web of Science ®Google Scholar
• Yildiz, D., Ercal, N., et al. (1998). Nicotine enantiomers and oxidative stress. Toxicology 130(2–3):155–165.
   PubMed Web of Science ®Google Scholar