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Research Article

In vitro comparative cytotoxicity study of aminated polystyrene, zinc oxide and silver nanoparticles on a cervical cancer cell line

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Pages 9-23 | Received 19 Oct 2017, Accepted 27 Dec 2017, Published online: 23 Jan 2018

References

  • Adeyemi, O.S. and Faniyan, T.O., 2014. Antioxidant status of rats administered silver nanoparticles orally. Journal of Taibah University Medical Sciences, 9 (3), 182–186.
  • Anguissola, S., et al., 2014. High content analysis provides mechanistic insights on the pathways of toxicity induced by amine-modified polystyrene nanoparticles. PLoS One, 9 (9), e108025.
  • Auffan, M., et al., 2009. Towards a definition of inorganic nanoparticles from an environmental, health and safety perspective. Nature Nanotechnology, 4 (10), 634–641.
  • Bexiga, M.G., et al., 2011. Cationic nanoparticles induce caspase 3-, 7- and 9-mediated cytotoxicity in a human astrocytoma cell line. Nanotoxicology, 5 (4), 557–567.
  • Brown, D.M., et al., 2004. Calcium and ROS-mediated activation of transcription factors and TNF-cytokine gene expression in macrophages exposed to ultrafine particles. American Journal of Physiology: Lung Cellular and Molecular Physiology, 286 (2), L344–L353.
  • Casey, A., et al., 2007. Spectroscopic analysis confirms the interactions between single walled carbon nanotubes and various dyes commonly used to assess cytotoxicity. Carbon, 45 (7), 1425–1432.
  • Casey, A., et al., 2008. Single walled carbon nanotubes induce indirect cytotoxicity by medium depletion in A549 lung cells. Toxicology Letters, 179 (2), 78–84.
  • Chairuangkitti, P., et al., 2013. Silver nanoparticles induce toxicity in A549 cells via ROS-dependent and ROS-independent pathways. Toxicology In Vitro, 27 (1), 330–338.
  • Chen, H.-W., et al., 2006. Titanium dioxide nanoparticles induce emphysema-like lung injury in mice. The FASEB Journal, 20 (13), 2393–2395.
  • Cho, W.-S., et al., 2011. Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn2+ dissolution inside lysosomes. Particle and Fibre Toxicology, 8 (1), 27.
  • Cohen, M.S., et al., 2007. In vitro analysis of a nanocrystalline silver-coated surgical mesh. Surgical Infections, 8 (3), 397–404.
  • Cooke, M.S., et al., 2003. Oxidative DNA damage: mechanisms, mutation, and disease. FASEB Journal, 17 (10), 1195–1214.
  • De Matteis, V., 2017. Exposure to inorganic nanoparticles: routes of entry, immune response, biodistribution and in vitro/in vivo toxicity evaluation. Toxics, 5 (4), E29.
  • Dingchao, C., Jie, Y., and Yanli, Z., 2004. Antibacterial materials of silver nanoparticles application in medical appliances and appliances for daily use. Chinese Medical Equipment Journal, 25 (11), 27–30.
  • Efeoglu, E., Casey, A., and Byrne, H., 2016. In vitro monitoring of time and dose dependent cytotoxicity of aminated nanoparticles using Raman spectroscopy. The Analyst, 141, 5417–5431.
  • Everett, W.N., et al., 2014. Phosphate-enhanced cytotoxicity of zinc oxide nanoparticles and agglomerates. Toxicology Letters, 225 (1), 177–184.
  • Fang, J.L. and Beland, F.A., 2009. Long-term exposure to zidovudine delays cell cycle progression, induces apoptosis, and decreases telomerase activity in human hepatocytes. Toxicological Sciences, 111 (1), 120–130.
  • Govender, R., et al., 2013. Silver nanoparticles of Albizia adianthifolia: the induction of apoptosis in human lung carcinoma cell line. Journal of Nanobiotechnology, 11 (1), 5.
  • Haynes, C.L., 2010. The emerging field of nanotoxicology. Analytical and Bioanalytical Chemistry, 398 (2), 587–588.
  • Hsiao, I.L. and Huang, Y.J., 2013. Effects of serum on cytotoxicity of nano- and micro-sized ZnO particles. Journal of Nanoparticle Research: An Interdisciplinary Forum for Nanoscale Science and Technology, 15, 1829.
  • Hsin, Y.H., et al., 2008. The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicology Letters, 179 (3), 130–139.
  • Hussain, S.M., et al., 2005. In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicology In Vitro: An International Journal Published in Association with Bibra, 19 (7), 975–983.
  • Jeong, S.H., et al., 2013. ZnO nanoparticles induce TNF-α expression via ROS-ERK-Egr-1 pathway in human keratinocytes. Journal of Dermatological Science, 72 (3), 263–273.
  • Kao, Y.Y., et al., 2012. Zinc oxide nanoparticles interfere with zinc ion homeostasis to cause cytotoxicity. Toxicological Sciences, 125 (2), 462–472.
  • Kasibhatla, S. and Tseng, B., 2003. Why target apoptosis in cancer treatment? Molecular Cancer Therapeutics, 2 (6), 573–580.
  • Khan, M., Naqvi, A.H., and Ahmad, M., 2015. Comparative study of the cytotoxic and genotoxic potentials of zinc oxide and titanium dioxide nanoparticles. Toxicology Reports, 2, 765–774.
  • Kim, S., et al., 2009. Oxidative stress-dependent toxicity of silver nanoparticles in human hepatoma cells. Toxicology In Vitro: An International Journal Published in Association with Bibra, 23 (6), 1076–1084.
  • Kovács, D., et al., 2016. Silver nanoparticles defeat p53-positive and p53-negative osteosarcoma cells by triggering mitochondrial stress and apoptosis. Scientific Reports, 6, 27902.
  • Kowaltowski, A.J., et al., 2009. Mitochondria and reactive oxygen species. Free Radical Biology & Medicine, 47 (4), 333–343.
  • Lansdown, A.B.G., 2006. Silver in health care: antimicrobial effects and safety in use. Current Problems in Dermatology, 33, 17–34.
  • Lee, Y.S., et al., 2011. Silver nanoparticles induce apoptosis and G2/M arrest via PKC??-dependent signaling in A549 lung cells. Archives of Toxicology, 85 (12), 1529–1540.
  • Lundqvist, M., et al., 2008. Nanoparticle size and surface properties determine the protein corona with possible implications for biological impacts. Proceedings of the National Academy of Sciences of the United States of America, 105 (38), 14265–14270.
  • Lunov, O., et al., 2011. Amino-functionalized polystyrene nanoparticles activate the NLRP3 inflammasome in human macrophages. ACS Nano, 5 (12), 9648–9657.
  • Lynch, I. and Dawson, K.A., 2008. Protein-nanoparticle interactions. Nano Today, 3 (1), 40–47.
  • Maher, M.A., et al., 2014. Numerical simulations of in vitro nanoparticle toxicity – the case of poly(amido amine) dendrimers. Toxicology In Vitro, 28 (8), 1449–1460.
  • McShan, D., Ray, P.C., and Yu, H., 2014. Molecular toxicity mechanism of nanosilver. Journal of Food and Drug Analysis, 22 (1), 116–127.
  • Miyayama, T. and Matsuoka, M., 2016. Involvement of lysosomal dysfunction in silver nanoparticle-induced cellular damage in A549 human lung alveolar epithelial cells. Journal of Occupational Medicine and Toxicology (Toxicology), 11, 1.
  • Mosmann, T., 1983. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. Journal of Immunological Methods, 65 (1–2), 55–63.
  • Mu, Q., et al., 2012. Mechanism of cellular uptake of genotoxic silica nanoparticles. Particle and Fibre Toxicology, 9 (1), 29.
  • Muangman, P., et al., 2006. Comparison of efficacy of 1% silver sulfadiazine and Acticoat for treatment of partial-thickness burn wounds. Journal of the Medical Association of Thailand, Chotmaihet Thangphaet, 89 (7), 953–958.
  • Mukherjee, S.G., et al., 2012. Comparative in vitro cytotoxicity study of silver nanoparticle on two mammalian cell lines. Toxicology In Vitro, 26 (2), 238–251.
  • Mukherjee, S.P., Davoren, M., and Byrne, H.J., 2010. In vitro mammalian cytotoxicological study of PAMAM dendrimers – towards quantitative structure activity relationships. Toxicology In Vitro, 24 (1), 169–177.
  • Murphy, A., et al., 2015a. Potential of biofluid components to modify silver nanoparticle toxicity. Journal of Applied Toxicology, 35 (6), 665–680.
  • Murphy, A., et al., 2015b. The surfactant dipalmitoylphophatidylcholine modifies acute responses in alveolar carcinoma cells in response to low-dose silver nanoparticle exposure. Journal of Applied Toxicology, 35 (10), 1141–1149.
  • Murphy, A., et al., 2016. Silver nanoparticles induce pro-inflammatory gene expression and inflammasome activation in human monocytes. Journal of Applied Toxicology, 36 (10), 1311–1320.
  • Naha, P.C., et al., 2009. Preparation, characterization of NIPAM and NIPAM/BAM copolymer nanoparticles and their acute toxicity testing using an aquatic test battery. Aquatic Toxicology, 92 (3), 146–154.
  • Nel, A., et al., 2006. Toxic potential of materials at the nanolevel. Science (New York, N.Y.), 311 (5761), 622–627.
  • O’Brien, J., et al., 2000. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry, 267 (17), 5421–5426.
  • Paget, V., et al., 2015. Specific uptake and genotoxicity induced by polystyrene nanobeads with distinct surface chemistry on human lung epithelial cells and macrophages. PLoS One, 10 (4), e0123297.
  • Piao, M.J., et al., 2011. Silver nanoparticles induce oxidative cell damage in human liver cells through inhibition of reduced glutathione and induction of mitochondria-involved apoptosis. Toxicology Letters, 201 (1), 92–100.
  • Pokhrel, S., et al., 2009. Comparison of the mechanism of toxicity of binary and mixed binary metal oxide nanoparticles based on dissolution and oxidative stress properties. Chemie Ingenieur Technik, 81 (8), 1167–1167.
  • Premanathan, M., et al., 2011. Selective toxicity of ZnO nanoparticles toward Gram-positive bacteria and cancer cells by apoptosis through lipid peroxidation. Nanomedicine: Nanotechnology, Biology and Medicine, 7 (2), 184–192.,
  • Roiter, Y., et al., 2008. Interaction of nanoparticles with lipid membrane. Nano Letters, 8 (3), 941–944.
  • Ruenraroengsak, P., et al., 2012. Respiratory epithelial cytotoxicity and membrane damage (holes) caused by amine-modified nanoparticles. Nanotoxicology, 6 (1), 94–108.
  • Saini, P., et al., 2016. Evidence of reactive oxygen species (ROS) mediated apoptosis in Setaria cervi induced by green silver nanoparticles from Acacia auriculiformis at a very low dose. Experimental Parasitology, 160, 39–48.
  • Saptarshi, S.R., Duschl, A., and Lopata, A.L., 2013. Interaction of nanoparticles with proteins: relation to bio-reactivity of the nanoparticle. Journal of Nanobiotechnology, 11 (1), 26.
  • Saptarshi, S.R., Duschl, A., and Lopata, A.L., 2015. Biological reactivity of zinc oxide nanoparticles with mammalian test systems: an overview. Nanomedicine, 10 (13), 2075–2092.
  • Sastre, F.V., et al., 2000. Mitochondrial oxidative stress plays a key role in aging and apoptosis. IUBMB Life (International Union of Biochemistry and Molecular Biology: Life), 49 (5), 427–435.
  • Senapati, V.A., et al., 2017. Zinc oxide nanoparticle induced age dependent immunotoxicity in BALB/c mice. Toxicology Research, 6 (3), 342–352.
  • Shalini, D., Senthilkumar, S., and Rajaguru, P., 2017. Effect of size and shape on toxicity of zinc oxide (ZnO) nanomaterials in human peripheral blood lymphocytes. Toxicology Mechanisms and Methods, 28 (2), 87–94.
  • Shang, L., et al., 2014. Nanoparticles interacting with proteins and cells: a systematic study of protein surface charge effects. Advanced Materials Interfaces, 1 (2), 1300079.
  • Shapero, K., et al., 2011. Time and space resolved uptake study of silica nanoparticles by human cells. Molecular BioSystems, 7 (2), 371–378.
  • Shi, Y., et al., 2000. Structural and biochemical basis of apoptotic activation by Smac/DIABLO. Nature, 406 (6798), 855–862.
  • Singh, R.P. and Ramarao, P., 2012. Cellular uptake, intracellular trafficking and cytotoxicity of silver nanoparticles. Toxicology Letters, 213 (2), 249–259.
  • Sotiriou, G.A. and Pratsinis, S.E., 2010. Antibacterial activity of nanosilver ions and particles. Environmental Science & Technology, 44 (14), 5649–5654.
  • Stennicke, H.R., et al., 1998. Pro-caspase-3 is a major physiologic target of caspase-8. Journal of Biological Chemistry, 273 (42), 27084–27090.
  • Sur, I., et al., 2012. The influence of the surface chemistry of silver nanoparticles on cell death. Nanotechnology, 23 (37), 375102
  • Treuel, L., Jiang, X., and Nienhaus, G.U., 2013. New views on cellular uptake and trafficking of manufactured nanoparticles. Journal of the Royal Society Interface, 10 (82), 20120939–20120939.
  • Valko, M., et al., 2004. Role of oxygen radicals in DNA damage and cancer incidence. Molecular and Cellular Biochemistry, 266 (1–2), 37–56.
  • Vamanu, C.I., et al., 2008. Induction of cell death by TiO2 nanoparticles: Studies on a human monoblastoid cell line. Toxicology In Vitro, 22 (7), 1689–1696.
  • Vila, A., et al., 2002. Design of biodegradable particles for protein delivery. Journal of Controlled Release, 78 (1–3), 15–24.
  • Vishwakarma, V., Samal, S.S., and Manoharan, N., 2010. Safety and risk associated with nanoparticles – a review. Journal of Minerals and Materials Characterization and Engineering, 9 (5), 455–459.
  • Wang, F., et al., 2013. The biomolecular corona is retained during nanoparticle uptake and protects the cells from the damage induced by cationic nanoparticles until degraded in the lysosomes. Nanomedicine: Nanotechnology, Biology and Medicine, 9 (8), 1159–1168.
  • Warheit, D.B., 2008. How meaningful are the results of nanotoxicity studies in the absence of adequate material characterization? Toxicological Sciences, 101 (2), 183–185.
  • White, M.J., DiCaprio, M.J., and Greenberg, D.A., 1996. Assessment of neuronal viability with Alamar blue in cortical and granule cell cultures. Journal of Neuroscience Methods, 70 (2), 195–200.
  • Wilhelmi, V., et al., 2012. Evaluation of apoptosis induced by nanoparticles and fine particles in RAW 264.7 macrophages: facts and artefacts. Toxicology In Vitro: An International Journal Published in Association with Bibra, 26 (2), 323–334.
  • Xia, T., et al., 2008. Cationic polystyrene nanosphere toxicity depends on cell-specific endocytic and mitochondrial injury pathways. ACS Nano, 2 (1), 85–96.
  • Yan, Z., et al., 2014. Transcriptional and posttranscriptional regulation and endocytosis were involved in zinc oxide nanoparticle-induced interleukin-8 overexpression in human bronchial epithelial cells. Cell Biology and Toxicology, 30 (2), 79–88.
  • Yang, Q. and Ma, Y., 2014. Irradiation-enhanced cytotoxicity of zinc oxide nanoparticles. International Journal of Toxicology, 33 (3), 187–203.
  • Yu, K.-N., et al., 2013. Zinc oxide nanoparticle induced autophagic cell death and mitochondrial damage via reactive oxygen species generation. Toxicology In Vitro, 27 (4), 1187–1195.
  • Zhang, J., et al., 2017. Zinc oxide nanoparticles harness autophagy to induce cell death in lung epithelial cells. Cell Death and Disease, 8 (7), e2954.
  • Zhang, L., et al., 2009. Efficient activation of p53 pathway in A549 cells exposed to L2, a novel compound targeting p53-MDM2 interaction. Anti-Cancer Drugs, 20 (6), 416–424.
  • Zhang, T., et al., 2014. Cytotoxic potential of silver nanoparticles. Yonsei Medical Journal, 55 (2), 283–291.
  • Zhao, X., et al., 2016. Zinc oxide nanoparticles induce oxidative DNA damage and ROS-triggered mitochondria-mediated apoptosis in zebrafish embryos. Aquatic Toxicology, 180, 56–70.
  • Zhu, B., et al., 2016. Silver nanoparticles induce HePG-2 cells apoptosis through ROS-mediated signaling pathways. Nanoscale Research Letters, 11 (1), 198.

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