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Research Article

Comparative toxicity of selected PAHs in rainbow trout hepatocytes: genotoxicity, oxidative stress and cytotoxicity

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Pages 71-78 | Received 17 Apr 2018, Accepted 29 Jun 2018, Published online: 24 Sep 2018

References

  • Andersson, T., Förlin, L., and Hansson, T., 1983. Biotransformation of 7-ethoxycoumarin in isolated perfused rainbow trout liver. Drug Metabolism and Disposition: The Biological Fate of Chemicals, 11 (5), 494–498.
  • Au, D.W.T., et al., 1999. Relationship between ultrastructural changes and EROD activities in liver of fish exposed to Benzo[a]pyrene. Environmental Pollution, 104 (2), 235–247.
  • Berry, M.N., and Friend, D.S., 1969. High-yield preparation of isolated rat liver parenchymal cells: a biochemical and fine structural study. The Journal of Cell Biology, 43 (3), 506–520.
  • Cavalieri, E.L., Devanesan, P.D., and Rogan, E.G., 1988. Radical cations in the horseradish peroxidase and prostaglandin H synthase mediated metabolism and binding of benzo[a]pyrene to deoxyribonucleic acid. Biochemical Pharmacology, 37 (11), 2183–2187.
  • Cavalieri, E.L., and Rogan, E.G., 1992. The approach to understanding aromatic hydrocarbon carcinogenesis. The central role of radical cations in metabolic activation. Pharmacology and Therapeutics, 55 (2), 183–199.
  • Collins, A.R., Ma, A.G., and Duthie, S.J., 1995. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutation Research, 336 (1), 69–77.
  • Cook, P.R., Brazell, I.A., and Jost, E., 1976. Characterization of nuclear structures containing superhelical DNA. Journal of Cell Science, 22 (2), 303–324.
  • De Maagd, P. G.-J., and Vethaak, A. D., 1998. Biotransformation of PAHs and their carcinogenic effects in fish. In: A.H. Nelson, ed. PAHs and related compounds. Berlin, Heidelberg: Springer-Verlag, 267–300.
  • Devaux, A., Pesonen, M., and Monod, G., 1997. Alkaline comet assay in rainbow trout hepatocytes. Toxicology in Vitro: An International Journal Published in Association with Bibra, 11 (1/2), 71–79.
  • Ellesat, K.S., et al., 2010. Cytotoxicity of atorvastatin and simvastatin on primary rainbow trout (Oncorhynchus mykiss) hepatocytes. Toxicology in Vitro, 24 (6), 1610–1618.
  • Ellesat, K.S., et al., 2011. Species-dependent sensitivity to contaminants: an approach using primary hepatocyte cultures with three marine fish species. Marine Environmental Research, 72 (4), 216–224.
  • Farmen, E., et al., 2010a. Produced water extracts from North Sea oil production platforms result in cellular oxidative stress in a rainbow trout in vitro bioassay. Marine Pollution Bulletin, 60 (7), 1092–1098.
  • Farmen, E., et al., 2010b. Oxidative stress responses in rainbow trout (Oncorhynchus mykiss) hepatocytes exposed to pro-oxidants and a complex environmental sample. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 151 (4), 431–438.
  • Ferraris, M., et al., 2002. Early oxidative damage in primary cultured trout hepatocytes: a time course study. Aquatic Toxicology, 59 (3/4), 283–296.
  • Frenzilli, G., et al., 2008. Cellular responses in the cyprinid Leuciscus cephalus from a contaminated freshwater ecosystem. Aquatic Toxicology, 89 (3), 188–196.
  • Gravato, C., and Santos, M.A., 2002. Juvenile sea bass liver P450, EROD induction, and erythrocytic genotoxic responses to PAH and PAH-like compounds. Ecotoxicology and Environmental Safety, 51 (2), 115–127.
  • Grung, M., et al., 2009. Polycyclic aromatic hydrocarbon (PAH) metabolites in Atlantic cod exposed via water or diet to a synthetic produced water. Journal of Toxicology and Environmental Health, 72 (3/4), 254–265.
  • Gualtieri, M., et al., 2011. Airborne urban particles (Milan winter-PM2.5) cause mitotic arrest and cell death: effects on DNA, mitochondria, AhR binding and spindle organization. Mutation Research – Fundamental and Molecular Mechanisms of Mutagen, 713 (1/2), 18–31.
  • Guengerich, F.P., 2000. Metabolism of chemical carcinogens. Carcinogenesis, 21 (3), 345–351.
  • Gutzkow, K.B., et al., 2013. High-throughput comet assay using 96 minigels. Mutagenesis, 28 (3), 333–340.
  • Harvey, R.G., 1991. Polycyclic aromatic hydrocarbons: chemistry and carcinogenicity. Cambridge, UK: Cambridge University Press.
  • Hemnani, T., and Parihar, M., 1998. Reactive oxygen species and oxidative DNA damage. Indian Journal of Physiology and Pharmacology, 42 (4), 440–452.
  • Holth, T.F., et al., 2009. Genotoxicity of environmentally relevant concentrations of water-soluble oil components in cod (Gadus morhua). Environmental Science & Technology, 43 (9), 3329–3334.
  • Incardona, J.P., Collier, T.K., and Scholz, N.L., 2004. Defects in cardiac function precede morphological abnormalities in fish embryos exposed to polycyclic aromatic hydrocarbons. Toxicology and Applied Pharmacology, 196 (2), 191–205.
  • Incardona, J.P., et al., 2006. Developmental toxicity of 4-ring polycyclic aromatic hydrocarbons in zebrafish is differentially dependent on AH receptor isoforms and hepatic cytochrome P4501A metabolism. Toxicology and Applied Pharmacology, 217 (3), 308–321.
  • Ingram, A.J., Phillips, J.C., and Lee, R., 1995. DNA adduct formation by mineral oils and their fractions as indicated by 32P-postlabelling: is adduct formation truly indicative of carcinogenic potential? Journal of Applied Toxicology, 15 (4), 275–283.
  • Jos, A., et al., 2009. The antioxidant glutathione in the fish cell lines EPC and BCF-2: response to model pro-oxidants as measured by three different fluorescent dyes. Toxicology in Vitro, 23 (3), 546–553.
  • Keyse, S., 1993. The induction of gene expression in mammalian cells by radiation. Seminars in Cancer Biology, 4 (2), 119–128.
  • Kienzler, A., et al., 2013a. Assessment of base-excision repair activity in fish cell lines: toward a new biomarker of exposure to environmental contaminants? Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 753 (2), 107–113.
  • Kienzler, A., Bony, S., and Devaux, A., 2013b. DNA repair activity in fish and interest in ecotoxicology: a review. Aquatic Toxicology, 134, 47–56.
  • Kim, K.B., and Lee, B.M., 1997. Oxidative stress to DNA, protein, and antioxidant enzymes (superoxide dismutase and catalase) in rats treated with benzo(a)pyrene. Cancer Letters, 113 (1/2), 205–212.
  • Kleinow, K.M., Melancon, M.J., and Lech, J.J., 1987. Biotransformation and induction: implications for toxicity, bioaccumulation and monitoring of environmental xenobiotics in fish. Environmental Health Perspectives, 71, 105–119.
  • Laville, N., et al., 2004. Effects of human pharmaceuticals on cytotoxicity, EROD activity and ROS production in fish hepatocytes. Toxicology, 196 (1/2), 41–55.
  • MacCrimmon, H.R., 1971. World distribution of rainbow trout (Salmo gairdneri). Journal of the Fisheries Research Board of Canada, 28 (5), 663–704.
  • Malins, D.C., et al., 1984. Chemical pollutants in sediments and diseases of bottom-dwelling fish in Puget Sound, Washington. Environmental Science & Technology, 18 (9), 705–713.
  • Malins, D.C., et al., 1985a. Toxic chemicals in marine sediment and biota from Mukilteo, Washington: relationships with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus). Journal of the National Cancer Institute, 74 (2), 487–494.
  • Malins, D.C., et al., 1985b. Toxic chemicals in sediments and biota from a creosote-polluted harbor: relationships with hepatic neoplasms and other hepatic lesions in English sole (Parophrys vetulus). Carcinogenesis, 6 (10), 1463–1469.
  • Marczynski, B., et al., 2005. Genotoxic risk assessment in white blood cells of occupationally exposed workers before and after alteration of the polycyclic aromatic hydrocarbon (PAH) profile in the production material: comparison with PAH air and urinary metabolite levels. International Archives of Occupational and Environmental Health, 78 (2), 97–108.
  • Mitchelmore, C.L., and Chipman, J.K., 1998. Detection of DNA strand breaks in brown trout (Salmo trutta) hepatocytes and blood cells using the single cell gel electrophoresis (comet) assay. Aquatic Toxicology, 41 (1/2), 161–182.
  • Morin, B., et al., 2011. Detection of DNA damage in yolk-sac larvae of the Japanese Medaka, Oryzias latipes, by the comet assay. Analytical and Bioanalytical Chemistry, 399 (6), 2235–2242.
  • Muñoz, B., and Albores, A., 2011. DNA damage caused by polycyclic aromatic hydrocarbons: mechanisms and markers. In: C.C. Chen, ed. Selected topics in DNA repair. Rijeka, Croatia: InTech, 125–144.
  • Musset, L., 2006. Current approaches in the statistical analysis of ecotoxicity data: a guidance to application. OCDE Series on Testing and Assessment, 54.
  • Myers, M.S., Johnson, L.L., and Collier, T.K., 2003. Establishing the causal relationship between polycyclic aromatic hydrocarbon (PAH) exposure and hepatic neoplasms and neoplasia-related liver lesions in English sole (Pleuronectes vetulus). Human and Ecological Risk Assessment: An International Journal, 9 (1), 67–94.
  • Neff, J. M., 1985. Polycyclic aromatic hydrocarbons. In: G.M. Rand and S.R. Petrocelli, eds. Fundamentals of aquatic toxicology: methods and applications. Washington, DC: Hemisphere Publishing Corporation, 416–454.
  • Neff, J. M., and Anderson, J. W., 1981. Response of marine animals to petroleum and specific petroleum hydrocarbons. Lomdon: Applied Science Publishers Ltd.
  • Neff, J.M., and Burns, W.A., 1996. Estimation of polycyclic aromatic hydrocarbon concentrations in the water column based on tissue residues in mussels and salmon: an equilibrium partitioning approach. Environmental Toxicology and Chemistry, 15 (12), 2240–2253.
  • Nehls, S., and Segner, H., 2001. Detection of DNA damage in two cell lines from rainbow trout, RTG-2 and RTL-W1, using the comet assay. Environmental Toxicology, 16 (4), 321–329.
  • Nehls, S., and Segner, H., 2005. Comet assay with the fish cell line rainbow trout gonad-2 for in vitro genotoxicity testing of xenobiotics and surface waters. Environmental Toxicology and Chemistry, 24 (8), 2078–2087.
  • Palanikumar, L., Kumaraguru, A.K., and Ramakritinan, C.M., 2013. Biochemical and genotoxic response of naphthalene to fingerlings of milkfish Chanos chanos. Ecotoxicology (London, England), 22 (7), 1111–1122.
  • Penning, T.M., 2004. Aldo-Keto reductases and formation of polycyclic aromatic hydrocarbon o-quinones. Methods in Enzymology, 378, 31–67.
  • Sandbacka, M., and Isomaa, B., 2000. Glutathione S-transferase and UDP-glucuronyltransferase activity in primary cultures of rainbow trout gill epithelial cells. Comparative Biochemistry and Physiology – Part C Toxicology and Pharmacology, 127 (3), 307–315.
  • Schirmer, K., Chan, A.G., and Bols, N.C., 2000. Transitory metabolic disruption and cytotoxicity elicited by benzo[a]pyrene in two cell lines from rainbow trout liver. Journal of Biochemical and Molecular Toxicology, 14 (5), 262–276.
  • Schirmer, K., et al., 1997. Methodology for demonstrating and measuring the photocytotoxicity of fluoranthene to fish cells in culture. Toxicology in Vitro : An International Journal Published in Association with Bibra, 11 (1/2), 107–119.
  • Schirmer, K., et al., 1998. Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill. Toxicology, 127 (1–3), 129–141.
  • Schreer, A., et al., 2005. Application of Alamar blue/5-carboxyfluorescein diacetate acetoxymethyl ester as a noninvasive cell viability assay in primary hepatocytes from rainbow trout. Analytical Biochemistry, 344 (1), 76–85.
  • Singh, N.P., et al., 1988. A simple technique for quantitation of low levels of DNA damage in individual cells. Experimental Cell Research, 175 (1), 184–191.
  • Slupphaug, G., Kavli, B., and Krokan, H.E., 2003. The interacting pathways for prevention and repair of oxidative DNA damage. Mutation Research, 531 (1/2), 231–251.
  • Smith, B.J., Curtis, J.F., and Eling, T.E., 1991. Bioactivation of xenobiotics by prostaglandin H synthase. Chemico-Biological Interactions, 79 (3), 245–264.
  • Sokal, R. R., and Rohlf, F. J., 1969. Biometry: the principles and practice of statistics in biological research. San Francisco: W. H. Freeman.
  • Sorg, O., 2004. Oxidative stress: a theoretical model or a biological reality?. Comptes Rendus Biologies, 327 (7), 649–662.
  • Stohs, S.J., Ohia, S., and Bagchi, D., 2002. Naphthalene toxicity and antioxidant nutrients. Toxicology, 180 (1), 97–105.
  • Teles, M., Pacheco, M., and Santos, M.A., 2003. Anguilla anguilla L. liver ethoxyresorufin O-deethylation, glutathione S-tranferase, erythrocytic nuclear abnormalities, and endocrine responses to naphthalene and β-naphthoflavone. Ecotoxicology and Environmental Safety, 55 (1), 98–107.
  • Thorgaard, G.H., et al., 2002. Status and opportunities for genomics research with rainbow trout. Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 133 (4), 609–646.
  • Tuvikene, A., 1995. Responses of fish to polycyclic aromatic hydrocarbons (PAHs). Annales Zoologici Fennici, 32, 295–309.
  • Valavanidis, A., et al., 2006. Molecular biomarkers of oxidative stress in aquatic organisms in relation to toxic environmental pollutants. Ecotoxicology and Environmental Safety, 64 (2), 178–189.
  • Wang, K., et al., 2002. Advantages of in vitro cytotoxicity testing by using primary rat hepatocytes in comparison with established cell lines. The Journal of Toxicological Sciences, 27 (3), 229–237.
  • Wessel, N., et al., 2012. Genotoxic and enzymatic effects of fluoranthene in microsomes and freshly isolated hepatocytes from sole (Solea solea). Aquatic Toxicology, 108, 33–41.
  • Wessel, N., et al., 2010. The toxicity of benzo[a]pyrene on sole (Solea solea) hepatocytes: assessment of genotoxic and enzymatic effects. Polycyclic Aromatic Compounds, 30 (5), 346–354.
  • Willett, K.L., et al., 2001. In vivo and in vitro inhibition of cyp1a-dependent activity in fundulus heteroclitus by the polynuclear aromatic hydrocarbon fluoranthene. Toxicology and Applied Pharmacology, 177 (3), 264–271.
  • Woo, S., et al., 2006. Comet assay for the detection of genotoxicity in blood cells of flounder (Paralichthys olivaceus) exposed to sediments and polycyclic aromatic hydrocarbons. Marine Pollution Bulletin, 52 (12), 1768–1775.
  • Wright, D.A., and Welbourn, P., 2002. Environmental toxicology. Cambridge, UK: Cambridge University Press.
  • Yazdani, M., and Hylland, K., 2016. A kinetic study of reactive oxygen species in rainbow trout hepatocytes by fluorometry. Journal of Environmental Pathology, Toxicology and Oncology, 35 (4), 291.
  • Yazdani, M., Santos Andresen, A.M., and Gjøen, T., 2016. Short-term effect of Bisphenol-A on oxidative stress responses in Atlantic salmon kidney cell line: a transcriptional study. Toxicology Mechanisms and Methods, 26 (4), 295–300.
  • Yazdani, M., et al., 2015. Reactive oxygen species and cytotoxicity in Rainbow trout hepatocytes: effects of medium and incubation time. Bulletin of Environmental Contamination and Toxicology, 94 (2), 193–198.
  • Zar, J., 1999. Biostatistical analysis. Upper Saddle River, NJ: Prentice Hall.
  • Zhang, J., et al., 2004. Effects of chronic exposure of 2,4-dichlorophenol on the antioxidant system in liver of freshwater fish Carassius auratus. Chemosphere, 55 (2), 167–174.

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