Comparison of several commonly used detection indicators of cell senescence

References

• Aird, K.M., et al., 2016. HMGB2 orchestrates the chromatin landscape of senescence-associated secretory phenotype gene loci. The Journal of Cell Biology, 215 (3), 325–334.
   PubMed Web of Science ®Google Scholar
• Aird, K.M., and Zhang, R., 2013. Detection of senescence-associated heterochromatin foci (SAHF). Methods in Molecular Biology (Clifton, N.J.), 965 (2), 185–196.
   PubMedGoogle Scholar
• Arun, P., et al., 2011. Senescence marker protein 30 (SMP30) expression in eukaryotic cells: existence of multiple species and membrane localization. Plos One, 6 (2), e16545
   PubMed Web of Science ®Google Scholar
• Baker, D.J., et al., 2011. Clearance of p16Ink4a-positive senescent cells delays ageing-associated disorders. Nature, 479 (7372), 232–236.
   PubMed Web of Science ®Google Scholar
• Bernadotte, A., Mikhelson, V.M., and Spivak, I.M., 2016. Markers of cellular senescence. Telomere shortening as a marker of cellular senescence. Aging, 8 (1), 3–11.
   PubMedGoogle Scholar
• Blasco, M.A., 2005. Telomeres and human disease: ageing, cancer and beyond. Nature reviews. Genetics, 6 (8), 611–622.
   PubMed Web of Science ®Google Scholar
• Brain, J.G., et al., 2013. Biliary epithelial senescence and plasticity in acute cellular rejection. American Journal of Transplantation, 13 (7), 1688–1702.
   PubMed Web of Science ®Google Scholar
• Burd, C.E., et al., 2013. Monitoring tumorigenesis and senescence in vivo with a p16(INK4a)-luciferase model. Cell, 152 (1–2), 340–351.
   PubMed Web of Science ®Google Scholar
• Burton, D.G., and Krizhanovsky, V., 2014. Physiological and pathological consequences of cellular senescence. Cellular and Molecular Life Sciences, 71 (22), 4373–4386.
   PubMed Web of Science ®Google Scholar
• Campisi, J., 2013. Aging, cellular senescence, and cancer. Annual Review of Physiology, 75, 685–705.
   PubMed Web of Science ®Google Scholar
• Campisi, J., and Fagagna, F., 2007. Cellular senescence: when bad things happen to good cells. Nature Reviews. Molecular Cell Biology, 8 (9), 729–740.
   PubMed Web of Science ®Google Scholar
• Chakraborty, S., et al., 2016. Cristacarpin promotes ER stress-mediated ROS generation leading to premature senescence by activation of p21 waf-1. Age, 38 (3), 1–14.
   PubMedGoogle Scholar
• Chen, W., et al., 2008. p53-related apoptosis resistance and tumor suppression activity in UVB-induced premature senescent human skin fibroblasts. International Journal of Molecular Medicine, 21 (5), 645–653.
   PubMed Web of Science ®Google Scholar
• Cristofalo, V.J., 2005. SA beta Gal staining: biomarker or delusion. Experimental Gerontology, 40 (10), 836–838.
   PubMed Web of Science ®Google Scholar
• Dimri, G.P., et al., 1995. A biomarker that identifies senescent human cells in culture and in aging skin in vivo. Proceedings of the National Academy of Sciences of the United States of America, 92 (20), 9363–9367.
   PubMed Web of Science ®Google Scholar
• Donate, L.E., and Blasco, M.A., 2011. Telomeres in cancer and ageing. Philosophical Transactions of the Royal Society of London. Series B Biological Sciences, 366 (1561), 76–84.
   PubMed Web of Science ®Google Scholar
• Fujita, T., 1999. Senescence marker protein-30 (SMP30): structure and biological function. Biochemical and Biophysical Research Communications, 254 (1), 1–4.
   PubMed Web of Science ®Google Scholar
• Ghosh, K., and Capell, B.C., 2016. The Senescence-associated secretory phenotype: critical effector in skin cancer and aging. Journal of Investigative Dermatology, 136 (11), 2133–2139.
   PubMed Web of Science ®Google Scholar
• Gorbunova, V., and Seluanov, A., 2016. DNA double strand break repair, aging and the chromatin connection. Mutation Research, 788, 2–6.
   PubMed Web of Science ®Google Scholar
• Green, M.R., 2008. Senescence: not just for tumor suppression. Cell, 134 (4), 562–564.
   PubMed Web of Science ®Google Scholar
• Harman, D., 1956. Aging: a theory based on free radical and radiation chemistry. Journal of Gerontology, 11 (3), 298–300.
   PubMedGoogle Scholar
• Hiyama, K., et al., 2006. Telomeres and Telomerase in Cancer. European Journal of Cancer, 42 (9), 1197–1203.
   PubMed Web of Science ®Google Scholar
• Johmura, Y., et al., 2014. Necessary and sufficient role for a mitosis skip in senescence induction. Molecular Cell, 55 (1), 73–84.
   PubMed Web of Science ®Google Scholar
• Jones, K.R., et al., 2005. p53-Dependent accelerated senescence induced by ionizing radiation in breast tumour cells. International Journal of Radiation Biology, 81 (6), 445–458.
   PubMed Web of Science ®Google Scholar
• Kapoor, V., and Telford, W.G., 2004. Telomere length measurement by fluorescence in situ hybridization and flow cytometry. Methods in Molecular Biology (Clifton, N.J.), 263 (2), 385–398.
   PubMedGoogle Scholar
• Kennedy, A.L., et al., 2010. Senescent mouse cells fail to overtly regulate the HIRA histone chaperone and do not form robust Senescence Associated Heterochromatin Foci. Cell Division, 5 (1), 16–27.
   PubMed Web of Science ®Google Scholar
• Kimura, M., et al., 2010. Measurement of telomere length by the Southern Blot analysis of terminal restriction fragment lengths. Nature Protocols, 5 (9), 1596–1607.
   PubMed Web of Science ®Google Scholar
• Kreiling, J.A., et al., 2011. Age-associated increase in heterochromatic marks in murine and primate tissues. Aging Cell, 10 (2), 292–304.
   PubMed Web of Science ®Google Scholar
• Kuilman, T., et al., 2008. Oncogene-induced senescence relayed by an interleukin-dependent inflammatory network. Cell, 133 (6), 1019–1031.
   PubMed Web of Science ®Google Scholar
• Lawless, C., et al., 2010. Quantitative assessment of markers for cell senescence. Experimental Gerontology, 45 (10), 772–778.
   PubMed Web of Science ®Google Scholar
• Li, Y., et al., 2013. Copper induces cellular senescence in human glioblastoma multiforme cells through downregulation of Bmi-1. Oncology Reports, 29 (5), 1805–1810.
   PubMed Web of Science ®Google Scholar
• Lu, S.Y., et al., 2006. Ripe areca nut extract induces G 1 phase arrests and senescence-associated phenotypes in normal human oral keratinocyte. Carcinogenesis, 27 (6), 1273–1284.
   PubMed Web of Science ®Google Scholar
• Lu, Y., et al., 2017. The interplay between p16 serine phosphorylation and arginine methylation determines its function in modulating cellular apoptosis and senescence. Scientific Reports, 7, 41390.
   PubMed Web of Science ®Google Scholar
• Masutomi, K., et al., 2005. The telomerase reverse transcriptase regulates chromatin state and DNA damage responses. Proceedings of the National Academy of Sciences of the United States of America, 102 (23), 8222–8227.
   PubMed Web of Science ®Google Scholar
• Matos, L., Gouveia, A., and Almeida, H., 2012. Copper ability to induce premature senescence in human fibroblasts. Age (Dordrecht, Netherlands), 34 (4), 783–794.
   PubMedGoogle Scholar
• Mohrin, M., and Chen, D., 2016. The mitochondrial metabolic checkpoint and aging of hematopoietic stem cells. Current Opinion in Hematology, 23 (4), 318–324.
   PubMed Web of Science ®Google Scholar
• Narita, M., et al., 2003. Rb-mediated heterochromatin formation and silencing of E2F target genes during cellular senescence. Cell, 113 (6), 703–716.
   PubMed Web of Science ®Google Scholar
• Niizuma, S., et al., 2012. Effect of persistent activation of phosphoinositide 3-kinase on heart. Life Sciences, 90 (15–16), 619–628.
   PubMed Web of Science ®Google Scholar
• O'Hagan-Wong, K., et al., 2016. Increased IL-6 secretion by aged human mesenchymal stromal cells disrupts hematopoietic stem and progenitor cells' homeostasis. Oncotarget, 7 (12), 13285–13296.
   PubMedGoogle Scholar
• Pal, H.C., et al., 2013. Delphinidin reduces cell proliferation and induces apoptosis of non-small-cell lung cancer cells by targeting EGFR/VEGFR2 signaling pathways. Plos One, 8 (10), e77270
   PubMed Web of Science ®Google Scholar
• Pati, S., et al., 2014. X-gal staining of canine skin tissues: a technique with multiple possible applications. Journal of Natural Science Biology and Medicine, 5 (2), 245–249.
   PubMedGoogle Scholar
• Petropoulou, C., et al., 2001. Clusterin/apolipoprotein J is a novel biomarker of cellular senescence that does not affect the proliferative capacity of human diploid fibroblasts. Febs Letters, 509 (2), 287–297.
   PubMed Web of Science ®Google Scholar
• Raghuram, G.V., and Mishra, P.K., 2014. Stress induced premature senescence: a new culprit in ovarian tumorigenesis? Indian Journal of Medical Research, 140(Suppl 1), S120–S129.
   PubMed Web of Science ®Google Scholar
• Rodier, F., 2013. Detection of the Senescence-Associated Secretory Phenotype (SASP). Methods in Molecular Biology, 965, 165–173.
   PubMedGoogle Scholar
• Sagiv, A., et al., 2013. Granule exocytosis mediates immune surveillance of senescent cells. Oncogene, 32 (15), 1971–1977.
   PubMed Web of Science ®Google Scholar
• Severino, J., et al., 2000. Is beta-galactosidase staining a marker of senescence in vitro and in vivo? Experimental Cell Research, 257 (1), 162–171.
   PubMed Web of Science ®Google Scholar
• Sorrentino, J.A., et al., 2014. p16INK4a reporter mice reveal age-promoting effects of environmental toxicants. Journal of Clinical Investigation, 124 (1), 169–173.
   PubMed Web of Science ®Google Scholar
• Sosińska, P., et al., 2014. Specificity of cytochemical and fluorescence methods of senescence-associated β-galactosidase detection for ageing driven by replication and time. Biogerontology, 15 (4), 407–413.
   PubMed Web of Science ®Google Scholar
• Sun, Q., and Zhang, H.B., 2008. Senescence is a defensive mechasim against oncogene-induced tumorigenesis. Basic and Clinical Medicine, 28 (5), 519–522.
   Google Scholar
• Thambisetty, M., et al., 2010. Association of plasma clusterin concentration with severity, pathology, and progression in Alzheimer disease. Archives of General Psychiatry, 67 (7), 739–748.
   PubMed Web of Science ®Google Scholar
• Trougakos, I.P., and Gonos, E.S., 2002. Clusterin/Apolipoprotein J in human aging and cancer. International Journal of Biochemistry and Cell Biology, 34 (11), 1430–1448.
   PubMed Web of Science ®Google Scholar
• Vaughan, S., and Jat, P.S., 2011. Deciphering the role of Nuclear Factor-κB in cellular senescence. Aging, 3 (10), 913–919. (
   PubMedGoogle Scholar
• Wang, C., et al., 2009. DNA damage response and cellular senescence in tissues of aging mice. Aging Cell, 8 (3), 311–323.
   PubMed Web of Science ®Google Scholar
• Wang, D., et al., 2016. Isolation of live premature senescent cells using FUCCI technology. Scientific Reports, 6, 30705.
   PubMed Web of Science ®Google Scholar
• Yumura, W., et al., 2006. Accelerated tubular cell senescence in SMP30 knockout mice. Histology and Histopathology, 21 (11), 1151–1156.
   PubMed Web of Science ®Google Scholar
• Zhang, S.C., et al., 2014. Inhibition of SMP30 gene expression influences the biological characteristics of human Hep G2 cells. Asian Pacific Journal of Cancer Prevention Apjcp, 15 (3), 1193–1196.
   PubMedGoogle Scholar
• Zhong, B., et al., 2010. Induction of clusterin by AKT—role in cytoprotection against docetaxel in prostate tumor cells. Molecular Cancer Therapeutics, 9 (6), 1831–1841.
   PubMed Web of Science ®Google Scholar
• Zhou, J., et al., 2015. HMGB1 induction of clusterin creates a chemoresistant niche in human prostate tumor cells. Scientific Reports, 5, 15085.
   PubMed Web of Science ®Google Scholar
• Zhou, S.F., et al., 2011. Serum immunoreactivity of SMP30 and its tissues expression in hepatocellular carcinoma. Clinical Biochemistry, 44 (4), 331–336.
   PubMed Web of Science ®Google Scholar