9
Views
13
CrossRef citations to date
0
Altmetric
Original Articles

A Rapid Isolation of Human Chorionic Gonadotropin and Its Subunits by Reversed-Phase High Performance Liquid Chromatography

, , , &
Pages 715-729 | Published online: 05 Dec 2006

References

  • Abbreviations: hCG, human chorionic gonadotropin; HPLC, high-performance liquid chromatography; TFA, trifluoroacetic acid; RIA, radioimmunoassay; SDS, sodium dodecyl sulfate; PFPA, pentafluoropropionic acid.
  • Morgan , F. J. , Birken , S. and Canfield , R. E. 1975 . The Amino Acid Sequence of Human Chorionic Gonadotropin: The α Subunit and [btilde] Subunit . J. Biol. Chem. , 250 : 5247
  • Birken , S. and Canfield , R. E. 1977 . Isolation and Amino Acid Sequence of COOH-terminal Fragments from the [btilde] Subunit of Human Chorionic Gonadotropin . J. Biol. Chem. , 252 : 5386
  • Keutmann , H. T. and Williams , R. M. 1977 . Human Chorionic Gonadotropin. Amino Acid Sequence of the Hormone-Specific COOH-Terminal Region . J. Biol. Chem. , 252 : 5393
  • Kessler , M. J. , Reddy , M. S. , Shah , R. H. and Bahl , O. P. 1979 . Structures of N-Glycosidic Carbohydrate Units of Human Chorionic Gonadotropin . J. Biol. Chem. , 254 : 7901
  • Kessler , M. J. , Mise , T. , Ghai , R. D. and Bahl , O. P. 1979 . Structure and Location of the O-Glycosidic Carbohydrate Units of Human Chorionic Gonadotropin . J. Biol. Chem. , 254 : 7909
  • Vaitukaitis , J. L. , Ross , G. T. , Braunstein , G. D. and Rayford , P. L. 1976 . Gonadotropins and Their Subunits: Basic and Clinical Studies . Recent Prog. Horm. Res. , 32 : 289
  • Ruddon , R. W. , Anderson , C. , Meade , K. S. , Aldenderfer , P. H. and Neuwald , P. D. 1979 . Content of Gonadotropins in Cultured Human Malignant Cells and Effects of Sodium Butyrate Treatment on Gonadotropin Secretion by HeLa Cells . Cancer Res. , 39 : 3885
  • Ruddon , R. W. , Hanson , C. A. , Bryan , A. H. , Putterman , G. J. , White , E. L. , Perini , F. , Meade , K. S. and Aldenderfer , P. H. 1980 . Synthesis and Secretion of Human Chorionic Gonadotropin Subunits by Cultured Human Malignant Cells . J. Biol. Chem. , 255 : 1000
  • Mahoney , W. C. and Hermodson , M. A. 1980 . Separation of Large Denatured Peptides by Reverse-Phase High Performance Liquid Chromatography. Trifluoroacetic Acid as a Peptide Solvent. J. Biol. Chem. , 255 : 11199
  • Henderson , L. E. , Sowder , R. and Oroszlan , S. 1981 . Proceedings of Int. Conf. on Chemical Synthesis and Sequencing of Peptides and Proteins , Edited by: Liu , D. T. , Schechter , A. N. and Heinrickson , R. Amsterdam : Elsevier . in, eds., in press
  • Bennett , H. P. J. , Browne , C. A. and Goltzman , D. 1979 . Proceedings 6th American Peptide Symposium , Edited by: Gross , E. and Meienhofer , J. 121 Rockford, IL : Pierce Chemical Company . in, eds., p.
  • Canfield , R. E. and Morgan , F. J. 1973 . Methods in Investigative Endocrinology , Edited by: Berson , S. A. and Yalow , R. S. Vol. 2B , 727 Amsterdam : North Holland Press . in, eds., Vol., p.
  • Morgan , F. J. , Canfield , R. E. , Vaitukaitis , J. L. and Ross , G. T. 1973 . Methods in Investigative Endocrinology , Edited by: Berson , S. A. and Yalow , R. S. Vol. 2B , 733 Amsterdam : North Holland Press . in, eds., p.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.