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Original Articles

Comparison of HPLC Separation of Vitamin D3 Metabolites and Their Isotachysterol3 Derivatives

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Pages 463-472 | Published online: 05 Dec 2006

References

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  • Trafford , D. J. H. , Seamark , D. A. , Turnbull , H. and Makin , H. L. 1981 . High-performance liquid chromatography of 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 in human plasma. Use of isotachysterols and comparison with gas chromatography-mass specrometry . J. Chromatogr. , 226 : 351
  • Seamark , D. A. , Trafford , D. J. H. and Makin , H. L. J. 1980 . A new procedure for the formation of isotachysterol derivatives of subnanomole quantities of ergocalciferol and cholecalciferol prior to gas-liquid chromatography . J. Steroid Biochem. , 13 : 1057
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  • Turnbull , H. , Trafford , D. J. H. and Makin , H. L. J. 1982 . A rapid and simple method for the measurement of plasma 25-hydroxyvitamin D2 and 25-hydroxyvitamin D3 using Sep-Pak C18 cartridges and a single high-performance liquid chromatographic step . Clin. Chim. Acta , 120 : 65
  • Brooks , C. J. W. , Brooks , R. V. , Fotherby , K. , Grant , J. K. , Klopper , A. and Klyne , W. 1970 . The identification of steroids . J. Endocrinol. , 47 : 265
  • Abbreviations: 25(OH)D3, 25-hydroxyvitamin D3; 24,25(OH)2D3,24,25-dihydroxyvitamin D3; 25,26(OH)2D3, 25,26-dihydroxyvitamin D3; 25(OH)D 3-lactone, 25-hydroxyvitamin D3-26,23-lactone; ITS3, isotachysterol3. 25(OH)ITS3, 24,25(OH)2ITS3, 25,26(OH)2-ITS3, 25(OH)ITS3-lactone, isotachysterol derivatives of the above vitamin D3, metabolites in respective order.

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