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Original Articles

Preparative Isolation by High Performance Liquid Chromatography of Human Insulin B Chain Produced in Escherichia Coli

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Pages 1517-1528 | Published online: 23 Oct 2006

References

  • Frank , B. H. and Chance , R. 1983 . Two routes for producing human insulin utilizing recombinant DNA technology . Munch. Med. Wschr. , 125 ( suppl. 1 ) : 514
  • James , L. D. 1984 . Scaling up bio-product separation with high performance liquid chromatography . Biotechnology , 2 : 957
  • Gail , S. and Charles , M. 1987 . From R & D to production: designing a chromatographic purification scheme . Biotechnology , 5 : 239
  • Ladrón de Guevara , O. , Estrada , G. , Antonio , S. , Alvarado , X. , Güereca , L. , Zamudio , F. and Bolivar , F. 1985 . Identification and isolation of human insulin A and B chains by high-performance liquid chromatograhy . J. Chromat , 349 : 91
  • Laemmli , U. K. 1970 . Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 : 680
  • Flores , N. , de Anda , R. , Güereca , L. , Cruz , N. , Antonio , S. , Balbás , P. , Bolivar , F. and Valle , F. 1986 . A new expression vector for the production of fused proteins in Escherichia coli . App. Microbiol. Biotechnol. , 25 : 267
  • Gross , E. 1967 . The cyanogen bromide reaction. Meth Enzymol. , 11 : 238
  • Cole , R. D. 1967 . Sulfhytolisis. Meth Enzymol , 11 : 206
  • Goeddel , D. V. , Kleid , D. F. , Bolivar , F. , Heyneker , H. L. , Yansura , D. G. , Crea , R. , Hirose , T. , Kraszewski , A. , Itakura , K. and Riggs , A. S.D. 1979 . Expression in Escherichia coli of chemically synthesized genes for human insulin . Proc Natl Acad Sci USA , 76 : 106
  • Balbás , P. , de Anda , R. , Flores , N. , Alvarado , X. , Cruz , N. , Valle , F. and Bolivar , F. 1988 . Overproduction of proteins by recombinant DNA: human insulin. Cell funtion and disease , Edited by: Cañedo , L. , Todd , L. , Jaz , J. and Parker , L. 59 New York, N. Y. : Plenum Press .

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