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Articles

Metagenomic Investigation of Bacterial and Archaeal Diversity of Hammam Essalihine Hot Spring from Khenchela, Algeria

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Pages 804-817 | Received 30 Jan 2020, Accepted 08 Jun 2020, Published online: 30 Jun 2020

References

  • AFNOR. 1999. Recueil de normes françaises: qualité de l’eau. 3rd éd. Paris, France: AFNOR.
  • Alauzet C, Jumas-Bilak E. 2014. The phylum Deferribacteres and the genus Caldithrix. In: Rosenberg E, DeLong EF, Lory S, Stackebrandt E, Thompson F, editors. The Prokaryotes. Berlin, Heidelberg, Germany: Springer.
  • Amarouche YS, Benouadah A, Bentabet EO, López GP. 2014. Morphological and phylogenetic diversity of thermophilic Cyanobacteria in Algerian hot springs. Extremophiles 18(6):1035–1047.
  • ANRH. 2006. Inventaire des points d’eaux willayas du Nord de l’Algérie. Document Interne ANRH, Bir Mourad Rais. Alger, Algéria.
  • Arab M, Bakour S, Lalaoui R, Aissaoui N, Nas F, Hoceini A, Fournier PE, Klouche-Khelil N. 2019. Diversity of aerobic bacilli analysis using molecular and culture-based approaches in debagh hot spring. Geomicrobiol J 36(2):137–117.
  • Barton LL, Fardeau M-L, Fauque GD. 2014. Hydrogen sulfide: a toxic gas produced by dissimilatory sulfate and sulfur reduction and consumed by microbial oxidation. Met Ions Life Sci 14:237–277.
  • Bergogne-Bérézin E, Towner KJ. 1996. Acinetobacter spp. as nosocomial pathogens: microbiological, clinical and epidemiological features. Clin Microbiol Rev 9(2):148–165.
  • Berkani C, Houha B. 2017. Physico-chemical and therapeutic characteristics of the thermo-mineral waters of Khenchela region (Northeastern Algeria). J Mater Environ Sci 8(5):1546–1553.
  • Brune A, Frenzel P, Cypionka H. 2000. Life at the oxic-anoxic interface: microbial activities and adaptations. FEMS Microbiol Rev 24(5):691–710.
  • Camacho C, Coulouris G, Avagyan V, Ma N, Papadopoulos J, Bealer K, Madden T. 2009. BLAST+: architecture and applications. BMC Bioinformatics 10(1):421.
  • Carbajo JM, Maraver F. 2017. Sulphurous mineral waters: new applications for health. Evid Based Complement Alternat Med 2017:8034084.
  • Chan CS, Chan KG, Ee R, Hong K-W, Urbieta MS, Donati ER, Shamsir MS, Goh KM. 2017. Effects of physiochemical factors on prokaryotic biodiversity in Malaysian circumneutral hot springs. Front Microbiol 8:1252.
  • Chan CS, Chan KG, Tay YL, Chua YH, Goh KM. 2015. Diversity of thermophiles in a Malaysian hot spring determined using 16S rRNA and shotgun metagenome sequencing. Front Microbiol 6:177.
  • Chen TL, Chou YJ, Chen WM, Arun B, Young CC. 2006. Tepidimonas taiwanensis sp. nov., a novel alkaline-protease-producing bacterium isolated from a hot spring. Extremophiles 10(1):35–40.
  • Chenaker H, Houha B, Valles V. 2017. Isotope studies and chemical investigations of hot Springs from North-Eastern Algeria. J Mater Environ Sci 8(12):4253–4263.
  • Chiriac CM, Szekeres E, Rudi K, Baricz A, Hegedus A, Dragoş N, Coman C. 2017. Differences in temperature and water chemistry shape distinct diversity patterns in thermophilic microbial communities. Appl Environ Microbiol 83(21):e01363–17.
  • Colegate SM, Molyneux RJ. 2008. Bioactive Natural Products: Detection, Isolation, and Structural Determination. 2nd ed. Boca Raton, FL: CRC Press.
  • Conrad G. 1983. Relation entre les eaux de surface et les eaux souterraines dans le Sahara (Sahara nord occidental), Algérie. Colloque Ressources en Eau et Utilisation. Constantine, Algeria: IST, p22.
  • Cox A, Shock EL, Havig JR. 2011. The transition to microbial photosynthesis in hot spring ecosystems. Chem Geo 280(3–4):344–351.
  • Daims H. 2014. The family Nitrospiraceae. In The Prokaryotes: Other Major Lineages of Bacteria and The Archaea. Rosenberg E., editor. p733–749.
  • Dunfield PF, Tamas I, Lee KC, Morgan XC, McDonald IR, Stott MB. 2012. Electing a candidate: a speculative history of the bacterial phylum OP10. Environ Microbiol 14(12):3069–3080.
  • Edwards T, Calica N, Huang D, Manoharan N, Hou W, Huang L, Panosyan H, Dong H, Hedlund BP. 2013. Cultivation and characterization of thermophilic Nitrospira species from geothermal springs in the US Great Basin, China, and Armenia. FEMS Microbiol Ecol 85(2):283–292.
  • Eriksen NT, Riis ML, Holm NK, Iversen N. 2011. H(2) synthesis from pentoses and biomass in Thermotoga spp. Biotechnol Lett 33(2):293–300.
  • Everroad RC, Otaki H, Matsuura K, Haruta S. 2012. Diversification of bacterial community composition along a temperature gradient at a thermal spring. Microbes Environ 27(4):374–381.
  • Frank YA, Kadnikov VV, Lukina AP, Banks D, Beletsky AV, Mardanov AV, Sen’kina EI, Avakyan MR, Karnachuk OV, Ravin NV. 2016. Characterization and genome analysis of the first facultatively alkaliphilic Thermodesulfovibrio isolated from the deep terrestrial subsurface. Front Microbiol 7:2000.
  • Freitas M, Rainey FR, Nobre MF, Silvestre AJD, da Costa MS. 2003. Tepidimonas aquatica sp. nov., a new slightly thermophilic b-proteobacterium isolated from a hot water tank, system. Appl Microbiol 26(3):376–381.
  • Gaisin VA, Kalashnikov AM, Grouzdev DS, Sukhacheva MV, Kuznetsov BB, Gorlenko VM. 2017. Chloroflexus islandicus sp. nov., a thermophilic filamentous anoxygenic phototrophic bacterium from a geyser). Int J Syst Evol Microbiol 67(5):1381–1386.
  • Garrity GM, Brenner DJ, Krieg NR, Staley JT, editors. 2005. Bergey’s Manual of Systematic Bacteriology. The Proteobacteria, Part C: The Alpha-, Beta-, Delta-, and Epsilonproteobacteria. Vol. 2. New York, NY: Springer.
  • Ghelani A, Patel R, Mangrola A, Dudhagara P. 2015. Cultivation-independent comprehensive survey of bacterial diversity in Tulsi Shyam Hot Springs, India. Genom Data 4:54–56.
  • Ghilamicael AM, Boga HI, Anami SE, Mehari T, Budambula N. 2018. Potential human pathogenic bacteria in five hot springs in Eritrea revealed by next generation sequencing. PLOS One 13(3):e0194554.
  • Ghilamicael AM, Budambula NLM, Anami SE, Mehari T, Boga HI. 2017. Evaluation of prokaryotic diversity of five hot springs in Eritrea. BMC Microbiol 17(1):203.
  • Gomri MA, Khaldi TM, Kharroub K. 2018. Analysis of the diversity of aerobic, thermophilic endospore-forming bacteria in two Algerian hot springs using cultural and non-cultural methods. Ann Microbiol 68(12):915–929.
  • Hammer Ø, Harper DAT, Ryan PD. 2001. PAST: paleontological statistics software package for education and data analysis. Palaeontol Electron 4(1):9.
  • Huber R, Stetter KO. 1992. The Thermotogales: hyperthermophilic and extremely thermophilic bacteria. In: Kristjansson JK, editor. Thermophilic Bacteria. London: CRC Press, p185–194.
  • Iino T, Mori K, Uchino Y, Nakagawa T, Harayama S, Suzuki KI. 2010. Ignavibacterium album gen. nov., sp. nov., a moderately thermophilic anaerobic bacterium isolated from microbial mats at a terrestrial hot spring and proposal of Ignavibacteria classis nov., for a novel lineage at the periphery of green sulfur bacteria. Int J Syst Evol Microbiol 60(6):1376–1382.
  • Iino T, Nakagawa T, Mori K, Harayama S, Suzuki KI. 2008. Calditerrivibrio nitroreducens gen. nov., sp. nov., a thermophilic, nitrate-reducing bacterium isolated from a terrestrial hot spring in Japan. Int J Syst Evol Microbiol 58(7):1675–1679.
  • Im WT, Hu ZY, Kim KH, Rhee SK, Meng H, Lee ST, Quan ZX. 2012. Description of Fimbriimonas ginsengisoli gen. nov., sp. nov. Within the Fimbriimonadia class nov., of the phylum Armatimonadetes. Antonie Van Leeuwenhoek 102(2):307–317.
  • Inskeep WP, Klatt CG, Herrgard MJ, Jay ZJ, Rusch DB, Tringe SG, Miller SR. 2013. Community structure and function of high-temperature chlorophototrophic microbial mats inhabiting diverse geothermal environments. Front Microbiol (4):106.
  • Jiang X. 2015. The impact of temperature, pH and environmental heterogeneity on prokaryotic diversity in Yellowstone National Park thermal Springs. Available at http://digitalrepository.unm.edu/biol_etds/57.
  • Kecha M, Benallaoua S, Touzel JP, Bonaly R, Duchiron F. 2007. Biochemical and phylogenetic characterization of a novel terrestrial hyperthermophilic archaeon pertaining to the genus Pyrococcus from an Algerian hydrothermal hot spring. Extremophiles 11(1):65–73.
  • Kulichevskaya IS, Suzina NE, Rijpstra WI, Sinninghe Damste JS, Dedysh SN. 2014. Paludibaculum fermentans gen. nov., sp. nov., a facultative anaerobe capable of dissimilatory iron reduction from subdivision 3 of the Acidobacteria. Int J Syst Evol Microbiol 64(8):2857–2864.
  • Kumar S, Stecher G, Tamura K. 2016. MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets. Mol Biol Evol 33(7):1870–1874.
  • Laville E, Ufarté L, Veronese G. 2015. Découverte de nouvelles fonctions et familles protéiques: nouveaux défis pour les biotechnologies et l’écologie microbienne. La métagénomique : développements  et futures applications, Editions Quae, p 35.
  • Lavrentyeva EV, Radnagurueva AA, Barkhutova DD, Belkova NL, Zaitseva SV, Namsaraev ZB, Gorlenko VM, Namsaraev BB. 2018. Bacterial diversity and functional activity of microbial communities in hot Springs of the baikal rift zone1. Microbiology 87 (2):272–281.
  • Lee SH, Cho JC. 2009. Distribution patterns of the members of phylum Acidobacteria in global soil samples. J Microbiol Biotech 19:1281–1287.
  • Lee KCY, Dunfield PF, Morgan XC, Crowe MA, Houghton KM, Vyssotski M, Ryan JLJ, Lagutin K, McDonald IR, Stott MB. 2011. Chthonomonas calidirosea gen. nov., sp nov., an aerobic, pigmented, thermophilic micro-organism of a novel bacterial class, Chthonomonadetes classis nov., of the newly described phylum Armatimonadetes originally designated candidate division OP10. Int J Syst Evol Microbiol 61(10):2482–2490.
  • Lehours AC. 2006. La communauté procaryotique dans les zones anoxiques de deux écosystèmes lacustres: structure et diversité. Etude plus particulière de son rôle fonctionnel dans le monimolimnion. Doctoral dissertation. Université Blaise Pascal - Clermont-Ferrand II, France.
  • Li H, Yang Q, Li J, Gao H, Li P, Zhou H. 2015. The impact of temperature on microbial diversity and AOA activity in the Tengchong Geothermal Field, China. Sci Rep 5:17056.
  • Liu Z, Frigaard N-U, Vogl K, Iino T, Ohkuma M, Overmann J, Bryant DA. 2012. Complete genome of Ignavibacterium album, a metabolically versatile, flagellated, facultative anaerobe from the phylum Chlorobi. Front Microbiol 3:185.
  • Liu Y, Whitman WB. 2008. Metabolic, phylogenetic, and ecological diversity of the methanogenic archaea. Ann NY Acad Sci 1125:171–189.
  • López-López O, Knapik K, Cerdán ME, González-Siso MI. 2015. Metagenomics of an alkaline hot spring in Galicia (Spain): microbial diversity analysis and screening for novel lipolytic enzymes. Front Microbiol 6:1291.
  • Losey NA, Stevenson BS, Busse HJ, Damsté JSS, Rijpstra WIC, Rudd S, Lawson PA. 2013. Thermoanaerobaculum aquaticum gen. nov., sp. nov., the first cultivated member of Acidobacteria subdivision 23, isolated from a hot spring. Int J Syst Evol Microbiol 63(11):4149–4157.
  • Lozupone CA, Knight R. 2007. Global patterns in bacterial diversity. Proc Natl Acad Sci USA 104(27):11436–11440.
  • Madigan MT, Martinko JM. 2007. Brock Biology of Microorganisms. Upper Saddle River, NJ: Pearson/Prentice Hall, p. 11.
  • Martinez-Canovas MJ, Bejar V, Martinez-Checa F, Paez R, Quesada E. 2004. Idiomarina fontislapidosi sp. nov. and Idiomarina ramblicola sp. nov., isolated from inland hypersaline habitats in Spain. Int J Syst Evol Microbiol 54(5):1793–1797.
  • Mehetre G, Shah M, Dastager SG, Dharne MS. 2018. Untapped bacterial diversity and metabolic potential within Unkeshwar hot springs, India. Arch Microbiol 200(5):753–770.
  • Mehta D, Satyanarayana T. 2013. Diversity of hot environments and thermophilic microbes. In: Satyanarayana, T, Littlechild, J, Kawarabayasi, Y, editors. Thermophilic Microbes in Environmental and Industrial Biotechnology. Dordrecht, Netherlands: Springer.
  • Menzel P, Gudbergsdóttir SR, Rike AG, Lin L, Zhang Q, Contursi P, Moracci M, Kristjansson JK, Bolduc B, Gavrilov S, et al. 2015. Comparative metagenomics of eight geographically remote terrestrial hot springs. Microb Ecol 70(2):411–424.
  • Nei M, Kumar S. 2000. Molecular Evolution and Phylogenetics. New York, NY: Oxford University Press.
  • Nivedita R, Joshi JK. 2019. Bacterial community structure analysis of a hot spring soil by next generation sequencing of ribosomal RNA. Genomics 111(5):1053–1058.
  • Ondov BD, Bergman NH, Phillippy AM. 2011. Interactive metagenomic visualization in a Web browser. BMC bioinformatics, 12(1), 385.
  • Ouali S. 2018. Eléments de l’atlas géothermique de l’algérie. Bulletin des energies renouvelables, Rapport Interne, CDER, N° 44. P. 11.
  • Panda AK, Bisht SS, Kaushal BR, De Mandal S, Kumar NS, Basistha BC. 2017. Bacterial diversity analysis of Yumthang hot spring, North Sikkim, India by Illumina sequencing. Big Data Anal 2(1):7.
  • Panosyan HH, Margaryan AA, Trchounian AH. 2018. Denaturing gradient gel electrophoresis (DGGE) profiles of the partial 16S rRNA genes defined bacterial population inhabiting in Armenian geothermal springs. Biol J Armenia 68(3):102–109.
  • Patel M, Daniel . 1985. Bergey’s Manual of Systematic Bacteriology. Vol. 1, 2nd ed. New York, NY: Springer, p375–376.
  • Podosokorskaya OA, Kadnikov VV, Gavrilov SN, Mardanov AV, Merkel AY, Karnachuk OV, Ravin NV, Bonch-Osmolovskaya EA, Kublanov IV. 2013. Characterization of Melioribacter roseus gen. nov., sp. nov., a novel facultatively anaerobic thermophilic cellulolytic bacterium from the class Ignavibacteria, and a proposal of a novel bacterial phylum Ignavibacteriae. Environ Microbiol 15(6):1759–1771.
  • Power JF, Carere CR, Lee CK, Wakerley GLJ, Evans DW, Button M, White D, Climo MD, Hinze AM, Morgan XC, et al. 2018. Microbial biogeography of 925 geothermal springs in New Zealand. Nat Commun 9(1):1–12.
  • Prieto-Barajas CM, Alfaro-Cuevas R, Valencia-Cantero E, Santoyo G. 2017. Effect of seasonality and physicochemical parameters on bacterial communities in two hot spring microbial mats from Araró, Mexico. Rev Mex Biodivers 88(3):616–624.
  • Pruesse E, Peplies J, Glöckner FO. 2012. SINA: accurate high throughput multiple sequence alignment of ribosomal rna genes. Bioinformatics 28(14):1823–1829.
  • Quast C, Pruesse E, Yilmaz P, Gerken J, Schweer T, Yarza P, Peplies J, Gl¨Ockner FO. 2013. The SILVA ribosomal RNA gene database project: improved data processing and web-based tools. Nucleic Acids Res 41:D590–D596.
  • Reysenbach, A-L. 2001. Bergey’s Manual of Systematic Bacteriology, Vol. 1, 2nd edn. New York, NY: Springer, p. 369.
  • Rozanov AS, Bryanskaya AV, Ivanisenko TV, Malup TK, Peltek SE. 2017. Biodiversity of the microbial mat of the Garga hot spring. BMC Evol Biol 17(S2):S5.
  • Saibi, H. 2015. Geothermal resources in Algeria. In: Proceedings in World Geothermal Congress 2015, Melbourne, Australia, 19–24 April.
  • Sayeh R, Birrien JL, Alain K, Barbier G, Hamdi M, Prieur D. 2010. Microbial diversity in Tunisian geothermal springs as detected by molecular and culture-based approaches. Extremophiles 14(6):501–514.
  • Schloss PD, Westcott SL, Ryabin T, Hall JR, Hartmann M, Hollister EB, Lesniewski RA, Oakley BB, Parks DH, Robinson CJ, et al. 2009. Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities. Appl Environ Microbiol 75(23):7537–7541.
  • Schreckenberger PC, Von Graevenitz A. 1999. Acinetobacter, Achromobacter, Alcaligenes, Moraxella, Methylobacterium, and other non fermentative Gram-negative rods. In: Murray PR, Baron EJ, Pfaller MA, Tenover FC, Yolken RH, editors. Manual of Clinical Microbiology. Washington, DC: American Society for Microbiology, p. 539–571.
  • Sekiguchi Y, Muramatsu M, Imachi H, Narihiro T, Ohashi A, Harada H, Hanada S, Kamagata Y. 2008. Thermodesulfovibrio aggregans sp. nov. and Thermodesulfovibrio thiophilus sp. nov., anaerobic, thermophilic, sulfate-reducing bacteria isolated from thermophilic methanogenic sludge, and emended description of the genus Thermodesulfovibrio. Int J Syst Evol Microbiol 58(11):2541–2548.
  • Sekiguchi Y, Yamada T, Hanada S, Ohashi A, Harada H, Kamagata Y. 2003. Anaerolinea thermophila gen. nov., sp. nov. and Caldilinea aerophila gen. nov., sp. nov., novel filamentous thermophiles that represent a previously uncultured lineage of the domain Bacteria at the subphylum level. Int J Syst Evol Microbiol 53(6):1843–1851.
  • Skirnisdottir S, Hreggvidsson GO, Hjörleifsdottir S, Marteinsson VT, Petursdottir SK, Holst O, Kristjansson JK. 2000. Influence of sulfide and temperature on species composition and community structure of hot spring microbial mats. Appl Environ Microbiol 66(7):2835–2841.
  • Song Z, Zhi X, Li W, Jiang H, Zhang C, Dong H. 2009. Actinobacterial diversity in Hot Springs in Tengchong (China), Kamchatka (Russia), and Nevada (USA). Geomicrobiol J 26(4):256–263.
  • Strunecký O, Kopejtka K, Goecke F, Tomasch J, Lukavský J, Neori A, Kahl S, Pieper DH, Pilarski P, Kaftan D, et al. 2019. High diversity of thermophilic cyanobacteria in Rupite hot spring identified by microscopy, cultivation, single-cell PCR and amplicon sequencing. Extremophiles 23(1):35–48.
  • Tamaki H, Tanaka Y, Matsuzawa H, Muramatsu M, Meng X-Y, Hanada S, Mori K, Kamagata Y. 2011. Armatimonas rosea gen. nov., sp. nov., of a novel bacterial phylum, Armatimonadetes phyl. nov., formally called the candidate phylum OP10. Int J Syst Evol Microbiol 61(6):1442–1447.
  • Tang J, Liang Y, Jiang D, Li L, Luo Y, Shah MMR, Daroch M. 2018. Temperature-controlled thermophilic bacterial communities in hot springs of western Sichuan. BMC Microbiol 18(1):134.
  • Tenreiro S, Nobre MF, Rainey FA, Miguel C, da Costa MS. 1997. Thermonema rossianum sp. nov., a new thermophilic and slightly halophilic species from saline hot springs in Naples, Italy. Int J Syst Bacteriol 47(1):122–126.
  • Thiel V, Garcia Costas AM, Fortney NW, Martinez JN, Tank M, Roden EE, Boyd ES, Ward DM, Hanada S, Bryant DA. 2018. “Candidatus thermonerobacter thiotrophicus,” a non-phototrophic member of the bacteroidetes/chlorobi with dissimilatory sulfur metabolism in hot spring mMat communities. Front Microbiol 9:3159.
  • Thiel V, Wood JM, Olsen MT, Tank M, Klatt CG, Ward DM, Bryant DA. 2016. The dark side of the mushroom spring microbial mat: life in the shadow of chlorophototrophs. I. microbial diversity based on 16S rRNA gene amplicons and metagenomic sequencing. Front Microbiol 7:919.
  • Tóth EM, Vengring A, Homonnay ZG, Kéki Z, Spröer C, Borsodi AK, Márialigeti K, Schumann P. 2014. Phreatobacter oligotrophus gen. nov., sp. nov., an Alphaproteobacterium isolated from ultrapure water of the water purification system of a power plant. Int J Syst Evol Microbiol 64(3):839–845.
  • Uribe-Lorio L, Brenes-Guillén L, Hernández-Ascencio W, Mora-Amador R, González G, Ramírez Umaña C, Diez B, Pedrós-Alió C. 2019. The influence of temperature and pH on bacterial community composition of microbial mats in hot springs from Costa Rica. Microbiologyopen 8:e893.
  • Ventosa A, Mellado E, Sanchez-Porro C, Marquez MC. 2008. Halophilic and halotolerant microorganisms from soils. In: Dion P, Nautiyal CS, editors. Microbiology of Extreme Soils. Soil Biology, Vol. 13. Berlin, Heidelberg: Springer.
  • Visca P, Petrucca A, De Mori P, Festa A, Boumis E, Antinori A, Petrosillo N. 2001. Community-acquired Acinetobacter radioresistens bacteremia in an HIV-positive patient. Emerging Infect Dis 7(6):1032–1035.
  • Wang S, Hou W, Dong H, Jiang H, Huang L, Wu G, Zhang C, Song Z, Zhang Y, Ren H, et al. 2013. Control of temperature on microbial community structure in hot springs of the Tibetan Plateau. PLOS One 8(5):e62901.
  • Wang Y, Qian PY. 2009. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. PLOS One 4(10):e7401.
  • Yang W. 2015. The bacterial communities of sand-like surface soils of the. Phd thesis, San Rafael Swell (Utah, USA) and the Desert of Maine (USA of PARIS-SACLAY University French).
  • Ward DM, Castenholz RW, and, Miller SR. 2012. Cyanobacteria in geothermal Habitats. In: Whitton BA, editor. Ecology of Cyanobacteria II: Their Diversity in Space and Time. Dordrecht, Netherlands: Springer, p. 39–63.
  • Wirth R, Ugele M, Wanner G. 2016. Motility and ultrastructure of Spirochaeta thermophila. Front Microbiol 7:1609.
  • Xia Y, Wang Y, Wang Y, Chin FYL, Zhang T. 2016. Cellular adhesiveness and cellulolytic capacity in Anaerolineae revealed by omics-based genome interpretation. Biotechnol Biofuels 9:111.
  • Xian WD, Narsing Rao MP, Zhou EM, Liu L, Xiao M, Li WJ. 2018. Diversity of thermophiles in terrestrial hot springs of Yunnan and Tibet, China. In: Egamberdieva D, Birkeland NK, Panosyan H, Li WJ, editors. Extremophiles in Eurasian Ecosystems: Ecology, Diversity, and Applications. Microorganisms for Sustainability, Vol. 8. Singapore: Springer.
  • Yamada T, Imachi H, Ohashi A, Harada H, Hanada S, Kamagata Y, Sekiguchi Y. 2007. Bellilinea caldifistulae gen. nov., sp. nov. and Longilinea arvoryzae gen. nov., sp. nov., strictly anaerobic, filamentous bacteria of the phylum Chloroflexi isolated from methanogenic propionate-degrading consortia. Int J Syst Evol Microbiol 57(10):2299–2306.
  • Yan YW, Jiang QY, Wang JG, Zhu T, Zou B, Qiu QF. 2018. Microbial communities and diversities in mudflat sediments analyzed using a modified metatranscriptomic method. Front Microbiol 9:1–15.

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