184
Views
2
CrossRef citations to date
0
Altmetric
ADSORPTION

A Single-Step Purification of the Glycoprotein of Nipah Virus Produced in Insect Cells using an Anion Exchange Chromatography Method

, , , &
Pages 249-257 | Received 22 Mar 2013, Accepted 22 Aug 2013, Published online: 17 Jan 2014

REFERENCES

  • Chua , K.B. ; Bellini , W.J. ; Rota , P.A. ; Harcourt , B.H. ; Tamin , A. ; Lam , S.K. ; Ksiazek , T.G. ; Rollin , P.E. ; Zaki , S.R. ; Shieh , W.J. ; Goldsmith , C.S. ; Roehrig , J.T. ; Eaton , B. ; Gould , A.R. ; Olson , J. ; Field , H. ; Daniel , P. ; Ling , A.E. ; Peters , C.J. ; Anderson , L.J. ; Mahy , B.W.J. ( 2000 ) Nipah virus: A recently emergent deadly paramyxovirus . Science , 288 : 1432 – 1435 .
  • Arankalle , V.A. ; Bandyopadhyay , B.T. ; Ramdasi , A.Y. ; Jadi , R. ; Patil , D.R. ; Rahman , M. ; Majumdar , M. ; Banerjee , P.S. ; Hati , A.K.; Goswami, R.P. ; Neogi , D.K. ; Mishra , A.C. ( 2011 ) Genomic characterization of Nipah Virus, West Bengal, India . Emerging Infectious Disease , 17 : 907 – 909 .
  • Chadha , M.S. ; Comer , J.A. ; Lowe , L. ; Rota , P.A. ; Rollin , P.E. ; Bellini , W.J. ; Ksiazek , T.G. ; Mishra , A.C. ( 2006 ) Nipah virus-associated encephalitis outbreak, Siliguri, India . Emerging Infectious Disease , 12 : 235 – 240 .
  • Lo , M.K. ; Lowe , L. ; Hummel , K.B. ; Sazzad , H.M.S. ; Gurley , E.S. ; Hossain , M.J. ; Luby , S.P. ; Miller , D.M. ; Comer , J.A. ; Rollin , P.E. ; Bellini , W.J. ; Rota , P.A. ( 2012 ) Characterization of Nipah Virus from outbreaks in Bangladesh, 2008–2010 . Emerging Infectious Disease , 18 : 248 – 255 .
  • Montgomery , J.M. ; Hossain , M.J. ; Gurley , E. ; Carroll , D.S. ; Croisier , A. ; Bertherat , E. ( 2008 ) Risk factors for Nipah virus encephalitis in Bangladesh . Emerging Infectious Disease , 14 : 1526 – 1532 .
  • Tee , K.K. ; Takebe , Y. ; Kamarulzaman , A. ( 2009 ) Emerging and re-emerging viruses in Malaysia, 1997–2007 . International Journal of Infectious Diseases , 13 : 307 – 318 .
  • Wang , L.F. ; Harcourt , B.H. ; Yu , M. ; Tamin , A. ; Rota , P.A. ; Bellini , W.J. ; Eaton , B.T. ( 2001 ) Molecular biology of Hendra and Nipah viruses . Microbes and Infection , 3 : 279 – 287 .
  • Tamin , A. ; Harcourt , B.H. ; Ksiazek , T.G. ; Rollin , P.E. ; Bellini , W.J. ; Rota , P.A. ( 2002 ) Functional properties of the fusion and attachment glycoproteins of Nipah virus . Virology , 296 : 190 – 200 .
  • Dillon , P.J. ; Parks , G.D. ( 2007 ) Role for the Phosphoprotein P subunit of the paramyxovirus polymerase in limiting induction of host cell antiviral responses . Journal of Virology , 81 : 11116 – 11127 .
  • Eshaghi , M. ; Tan , W.S. ; Ong , S.W. ; Yusoff , K. ( 2005a ) Purification and characterization of Nipah virus Nucleocapsid protein produced in insect cells . Journal of Clinical Microbiology , 43 : 3172 – 3177 .
  • Lamb , R.A. ; Parks , G.D. ( 2007 ) Paramyxoviridae: the viruses and their replication . In: Fields Virology, , 5th ed. ; vol. 1 , Lippincott Williams and Wilkins : Philadephia , PA , pp. 1449 – 1496 .
  • Bonaparte , M.I. ; Dimitrov , A.S. ; Bossart , K.N. ; Crameri , G. ; Mungall , B.A. ; Bishop , K.A. ; Choudhry , V. ; Dimitrov , D.S. ; Wang , L.F. ; Eaton , B.T. ; Broder , C.C. ( 2005 ) Ephrin-B2 ligand is a functional receptor for Hendra virus and Nipah virus. Proceedings of the National Academy of Sciences of the United States of America, vol. 102; pp. 10652–10657.
  • Bowden , T.A. ; Aricescu , A.R. ; Gilbert , R.J. ; Grimes , J.M. ; Jones , E.Y. ; Stuart , D.I. ( 2008a ) Structural basis of Nipah and Hendra virus attachment to their cell-surface receptor ephrin-B2 . Nature Structural & Molecular Biology , 15 : 567 – 572 .
  • Bishop , K.A. ; Stantchev , T.S. ; Hickey , A.C. ; Khetawat , D. ; Bossart , K.N. ; Krasnoperov , V. ; Gill , P. ; Feng , Y.U. ; Wang , L. ; Eaton , B.T. ; Wang , L.F. ; Broder , C.C. ( 2007 ) Identification of Hendra virus G glycoprotein residues that are critical for receptor binding . Journal of Virology , 81 : 5893 – 5901 .
  • Weingartl , H.M. ; Berhane , Y. ; Caswell , J.L. ; Loosmore , S. ; Audonnet , J.C. ; Roth , J.A. ; Czub , M. ( 2006 ) Recombinant Nipah virus vaccines protect pigs against challenge . Journal of Virology , 80 : 7929 – 7938 .
  • Tamin , A. ; Harcourt , B.H. ; Lo , M.K. ; Roth , J.A. ; Wolf , M.C. ; Lee , B. ; Weingartl , H. ; Audonnet , J.C. ; Bellini , W.J. ; Rota , P.A. ( 2009 ) Development of a neutralization assay for Nipah virus using pseudotype particles . The Journal of Virological Methods , 160 : 1 – 6 .
  • Bossart , K.N. ; Crameri , G. ; Dimitrov , A.S. ; Mungall , B.A. ; Feng , Y.R. ; Patch , J.R. ; Choudhary , A. ; Wang , L.F. ; Eaton , B.T. ; Broder , C.C. ( 2005 ) Receptor binding, fusion inhibition and induction of cross reactive neutralizing antibodies by a soluble G glycoprotein of Hendra virus . Journal of Virology , 79 : 6690 – 6702 .
  • McEachern , J.A. ; Bingham , J. ; Crameri , G. ; Green , D.J. ; Hancock , T.J. ; Middleton , D. ; Feng , Y.R. ; Broder , C.C. ; Wang , L.F. ; Bossart , K.N. ( 2008 ) A recombinant subunit vaccine formulation protects against lethal Nipah virus challenge in cats . Vaccine , 26 : 3842 – 3852 .
  • Mungall , B.A. ; Middleton , D. ; Crameri , G. ; Bingham , J. ; Halpin , K. ; Russell , G. ; Green , D. ; McEachern , J. ; Pritchard , I. ; Eaton , B.T. ; Wang , L.F. ; Bossart , K.N. ( 2006 ) Feline model of acute Nipah virus infection and protection with a soluble glycoprotein-based subunit . Journal of Virology , 80 : 12293 – 12302 .
  • Eshaghi , M. ; Tan , W.S. ; Mohidin , T.B.M. ; Yusoff , K. ( 2004 ) Nipah virus glycoprotein: Production in baculovirus and application in diagnosis . Virus Research , 106 : 171 – 176 .
  • Biering , S.B. ; Huang , A. ; Vu , A.T. ; Robinson , L.R. ; Bradel-Tretheway , B. ; Choi , E. ; Lee , B. ; Aguilar , H.C. ( 2012 ) N-Glycans on the Nipah virus attachment glycoprotein modulate fusion and viral entry as they protect against antibody neutralization . Journal of Virology , 86 : 11991 – 2002 .
  • Kakua , Y. ; Noguchi , A. ; Marsh , G.A. ; Barr , J.A. ; Okutani , A. ; Hott , K. ; Bazartseren , B. ; Fukushi , S. ; Broder , C.C. ; Yamada , A. ; Inoue , S. ; Wang , L.F. (2012) Second generation of pseudotype-based serum neutralization assay for Nipah virus antibodies: Sensitive and high-throughput analysis utilizing secreted alkaline phosphatase. Journal of Virological Methods , 179: 226–232.
  • Eshaghi , M. ; Tan , W.S. ; Kit , C.W. ; Yusoff , K. ( 2005b ) Purification of the extra-cellular domain of Nipah virus glycoprotein produced in Escherichia coli and possible application in diagnosis . Journal of Biotechnology , 116 : 221 – 226 .
  • Mellado , M.C.M. ; Peixoto , C. ; Cruza , P.E. ; Carrondo , M.J.T. ; Alves , P.M. ( 2008 ) Purification of recombinant rotavirus VP7 glycoprotein for the study of in vitro rotavirus-like particles assembly . Journal of Chromatography B , 874 : 89 – 94 .
  • Sellhorn , G. ; Kraft , Z. ; Caldwell , Z. ; Ellingson , K. ; Mineart , C. ; Seaman , M.S. ; Montefiori , D.C. ; Lagerquist , E. ; Stamatatos , L. ( 2012 ) Engineering, expression, purification, and characterization of Stable Clade A/B Recombinant Soluble Heterotrimeric gp140 proteins . Journal of Virology , 86 : 128 – 142 .
  • Tello , D. ; Rodriguez-Rodríguez , M. ; Yelamos , B. ; Gomez-Gutierrez , J. ; Ortega , S. ; Pacheco , B. ; Peterson , D.L. ; Gavilanes , F. ( 2010 ) Expression and structural properties of a chimeric protein based on the ectodomains of E1 and E2 hepatitis C virus envelope glycoproteins . Protein Expression and Purification , 71 : 123 – 131 .
  • Nettleship , J.E. ; Rahman-Huq , N. ; Owens , R.J. ( 2009 ) The production of glycoproteins by transient expression in mammalian cells . Methods of Molecular Biology , 9498 : 245 – 223 .
  • Huang , Y. ; Bi , L. ; Zhou , W. ; Li , Y. ; Wang , Y. ; Ma , G. ( 2006 ) Improving recovery of recombinant hepatitis B virus surface antigen by ion-exchange chromatographic supports with low ligand density . Process Biochemistry , 41 : 2320 – 2326 .
  • Wongchuphan , R. ; Tey , B.T. ; Tan , W.S. ; Subramanian , S.K. ; Taip , F.S. ; Ling , T.C. ( 2011 ) Purification of rabbit polyclonal immunoglobulin G using anion exchangers . Process Biochemistry , 46 : 101 – 107 .
  • Carlsson , J. ; Kuznar , M. ; Varga , E. ; Everitt , J. ( 1994 ) Purification of infectious pancreatic necrosis virus by anion exchange chromatography increases the specific infectivity . Virology Methods , 47 : 27 – 36 .
  • Prazeres , D.M.F. ; Schluep , T. ; Cooney , C. ( 1998 ) Preparative purification of supercoiled plasmid DNA using exchange chromatography . Journal of Chromatography A , 806 : 31 – 45 .
  • Goheen , S.C. ; Hilsenbeck , J.L. ( 1998 ) High-performance ion-exchange chromatography and adsorption of plasma proteins . Journal of Chromatography A , 816 : 89 – 96 .
  • Weitzhandler , M. ; Farnan , D. ; Horvath , J. ; Rohrer , J.S. ; Slingsby , R.W. ; Avdalovic , N. ; Poh , C. ( 1998 ) Protein variant separations by cation exchange chromatography on tentacle-type polymeric stationary phases . Journal of Chromatography A , 828 : 365 – 372 .
  • Hurum , D.C. ; Rohrer , J.S. ( 2011 ) Five-minute glycoprotein sialic acid determination by high-performance anion exchange chromatography with pulsed amperometric detection . Analytical Biochemistry , 419 : 67 – 69 .
  • Monjezi , R. ; Tey , B.T. ; Sieo , C.C. ; Tan , W.S. ( 2010 ) Purification of bacteriophage M13 by anion exchange chromatography . Journal of Chromatography B , 878 : 1855 – 1859 .
  • Greina , T.A. ; Ronald , M. ; Lopez , M.V. ; Czermak , P. ( 2012 ) Purification of a recombinant baculovirus of Autographa californica Mnucleopoly hedrovirus by ion exchange membrane chromatography . Journal of Virology Methods , 183 : 117 – 124 .
  • Damhof , R.A. ; Feijibrief , M. ; Welling-Wester , S. ; Welling , G.W. ( 1994 ) Purification of the integral membrane glycoproteins D of Herpes simplex virus types 1 and 2, produced in the recombinant baculovirus expression system, by ion-exchange high-performance liquid chromatography . Journal of Chromatography A , 676 : 43 – 49 .
  • Guo , W. ; Cleveland , B. ; Davenport , T.M. ; Lee , K.K. ; Hu , S.L. ( 2013 ) Purification of recombinant vaccinia virus-expressed monomeric HIV-1 gp120 to apparent homogeneity . Protein Expression and Purification , 90 : 34 – 39 .
  • Matanin , B.M. ; Huang , Y. ; Meng , X.J. ; Zhang , C. ( 2008 ) Purification of the major envelop protein GP5 of porcine reproductive and respiratory syndrome virus (PRRSV) from native virions . Journal of Virological Methods , 147 : 127 – 35 .
  • Haumont , M. ; Jacquet , A. ; Massaer , M. ; Deleersnyder , V. ; Mazzu , P. ; Bollen , A. ; Jacobs , P. ( 1996 ) Purification, characterization and immunogenicity of recombinant varicella-zoster virus glycoprotein gE secreted by Chinese hamster ovary cells . Virus Research , 40 : 199 – 204 .
  • Vicente , T. ; Sousa , M.F.Q. ; Peixoto , C. ; Mota , J.P.B. ; Alves , P.M. ; Carrondo , M.J.T. ( 2008 ) Anion-exchange membrane chromatography for purification of rotavirus-like particles . Journal of Membrane Science , 311 : 270 – 283 .
  • Recio , I. ; Visser , S. ( 1999 ) Two ion-exchange chromatographic methods for the isolation of antibacterial peptides from lactoferrin In situ enzymatic hydrolysis on an ion-exchange membrane . Journal of Chromatography A , 831 : 191 – 201 .
  • Tey , B.T. ; Yong , K.H. ; Ong , H.P. ; Ling , T.C. ; Ong , S.T. ; Tan , Y.P. ; Ariff , A. ; Tan , W.S. ( 2004 ) Optimal conditions for Hepatitis B core Antigen production in shaked flask fermentation . Biotechnology and Bioprocess Engineering , 9 : 374 – 378 .
  • LaemmLi , U.K. ( 1970 ) Cleavage of structural proteins during the assembly of the head of bacteriophage T4 . Nature , 227 : 680 – 685 .
  • Ng , M.Y.T. ; Tan , W.S. ; Abdullah , N. ; Ling , T.C. ; Tey , B.T. ( 2006 ) Heat treatment of unclarified Escherichia coli homogenate improved the recovery efficiency of recombinant hepatitis B core antigen . Journal of Virology Methods , 137 : 134 – 139 .
  • Bradford , M.M. ( 1976 ) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Analytical Biochemistry , 72 : 248 – 254 .
  • Bowden , T.A. ; Crispin , M. ; Harvey , D.J. ; Jones , E.Y. ; Stuart , D.I. ( 2010 ) Dimeric Architecture of the Hendra Virus attachment glycoprotein: Evidence for a conserved mode of assembly . Journal of Virology , 84 : 6208 – 6217 .
  • Zheng , Y. ; Tang , Y. ; Fu , Y. ; He , J. ; Wang , X. ; Hong , T. ( 2012 ) Purification of human respiratory syncytial virus fusion glycoprotein . Protein Expression and Purification , 81 : 115 – 118 .
  • Jones , D.H. ; McBride , B.W. ; Roff , M.A. ; Maloney , V. ; Farrar , G.H. ( 1994 ) Purification and characterization of simian immunodeficiency virus (SlVmac) envelope glycoprotein GPI30 from virus-infected cells . Vaccine , 12 : 250 – 258 .
  • Jones , D.H. ; McBride , B.W. ; Roff , M.A. ; Maloney , V. ; Farrar , G.H. ( 1995 ) Efficient purification and rigorous characterization of a recombinant gp120 for HIV vaccine studies . Vaccine , 13 : 991 – 999 .
  • Jacquet , A. ; Massaer , M. ; Haumont , M. ; Houard , S. ; Deleersnyder , V. ; Place , M. ; Bollen , A. ; Jacobs , P. ( 1995 ) Purification and characterization of recombinant Varicella-Zoster virus glycoprotein gpII, secreted by Chinese hamster ovary cells . Protein Expression and Purification , 6 : 91 – 98 .
  • Color versions of one or more of the figures in the article can be found online at www.tandfonline.com/lsst.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.