380
Views
36
CrossRef citations to date
0
Altmetric
Original Articles

Sulforaphane Induces DNA Damage and Mitotic Abnormalities in Human Osteosarcoma MG-63 Cells: Correlation with Cell Cycle Arrest and Apoptosis

, , , , , , & show all
Pages 325-334 | Received 16 May 2013, Accepted 04 Nov 2013, Published online: 09 Jan 2014

REFERENCES

  • Stiller , C A , Bielack , S S , Jundt , G and Steliarova-Foucher , E . 2006 . Bone tumours in European children and adolescents, 1978–1997. Report from the Automated Childhood Cancer Information System project . Eur J Cancer , 42 : 2124 – 2135 .
  • Ham , S J , Schraffordt Koops , H , van der Graaf , W T , van Horn , J R Postma , L . 1998 . Historical, current and future aspects of osteosarcoma treatment . Eur J Surg Oncol , 24 : 584 – 600 .
  • Gorlick , R and Khanna , C . 2010 . Osteosarcoma . J Bone Miner Res , 25 : 683 – 691 .
  • Ta , H T , Dass , C R , Larson , I , Choong , P FM and Dunstan , D E . 2009 . A chitosan-dipotassium orthophosphate hydrogel for the delivery of Doxorubicin in the treatment of osteosarcoma . Biomaterials , 30 : 3605 – 3613 .
  • PosthumaDeBoer , J , Witlox , M A , Kaspers , G JL and van Royen , B J . 2011 . Molecular alterations as target for therapy in metastatic osteosarcoma: a review of literature . Clin Exp Metastas , 28 : 493 – 503 .
  • Wachtel , M and Schafer , B W . 2010 . Targets for cancer therapy in childhood sarcomas . Cancer Treat Rev , 36 : 318 – 327 .
  • Broadhead , M L , Clark , J C , Myers , D E , Dass , C R and Choong , P F . 2011 . The molecular pathogenesis of osteosarcoma: a review . Sarcoma , : 959248 1–12, 2011
  • Clarke , J D , Hsu , A , Riedl , K , Bella , D Schwartz , S J . 2011 . Bioavailability and inter-conversion of sulforaphane and erucin in human subjects consuming broccoli sprouts or broccoli supplement in a cross-over study design . Pharmacol Res , 64 : 456 – 463 .
  • Gasper , A V , Al-janobi , A , Smith , J A , Bacon , J R Fortun , P . 2005 . Glutathione S-transferase M1 polymorphism and metabolism of sulforaphane from standard and high-glucosinolate broccoli . Am J Clin Nutr , 82 : 1283 – 1291 .
  • Brooks , J D , Paton , V G and Vidanes , G . 2001 . Potent induction of phase 2 enzymes in human prostate cells by sulforaphane . Cancer Epidemiol Biomarkers Prev , 10 : 949 – 954 .
  • Morimitsu , Y , Nakagawa , Y , Hayashi , K , Fujii , H Kumagai , T . 2002 . A sulforaphane analogue that potently activates the Nrf2-dependent detoxification pathway . J Biol Chem , 277 : 3456 – 3463 .
  • Huang , T Y , Chang , W C , Wang , M Y , Yang , Y R and Hsu , Y C . 2012 . Effect of sulforaphane on growth inhibition in human brain malignant glioma GBM 8401 cells by means of mitochondrial- and MEK/ERK-mediated apoptosis pathway . Cell Biochem Biophys , 63 : 247 – 259 .
  • Kallifatidis , G , Labsch , S , Rausch , V , Mattern , J Gladkich , J . 2011 . Sulforaphane Increases Drug-mediated Cytotoxicity Toward Cancer Stem-like Cells of Pancreas and Prostate . Mol Ther , 19 : 188 – 195 .
  • Kanematsu , S , Yoshizawa , K , Uehara , N , Miki , H Sasaki , T . 2011 . Sulforaphane inhibits the growth of KPL-1 human breast cancer cells in vitro and suppresses the growth and metastasis of orthotopically transplanted KPL-1 cells in female athymic mice . Oncol Rep , 26 : 603 – 608 .
  • Li , Y Y , Zhang , T , Korkaya , H , Liu , S L Lee , H F . 2010 . Sulforaphane, a dietary component of broccoli/broccoli sprouts, inhibits breast cancer stem cells . Clin Cancer Res , 16 : 2580 – 2590 .
  • Rausch , V , Liu , L , Kallifatidis , G , Baumann , B Mattern , J . 2010 . Synergistic Activity of Sorafenib and Sulforaphane Abolishes Pancreatic Cancer Stem Cell Characteristics . Cancer Res , 70 : 5004 – 5013 .
  • Singh , S V , Herman-Antosiewicz , A , Singh , A V , Lew , K L Srivastava , S K . 2004 . Sulforaphane-induced G2/M phase cell cycle arrest involves checkpoint kinase 2-mediated phosphorylation of cell division cycle 25C . J Biol Chem , 279 : 25813 – 25822 .
  • Herman-Antosiewicz , A , Xiao , H , Lew , K L and Singh , S V . 2007 . Induction of p21 protein protects against sulforaphane-induced mitotic arrest in LNCaP human prostate cancer cell line . Mol Cancer Ther , 6 : 1673 – 1681 .
  • Kim , M R , Zhou , L , Park , B H and Kim , J R . 2011 . Induction of G(2)/M arrest and apoptosis by sulforaphane in human osteosarcoma U2-OS cells . Mol Med Rep , 4 : 929 – 934 .
  • Shen , G , Xu , C , Chen , C , Hebbar , V and Kong , A N . 2006 . p53-independent G1 cell cycle arrest of human colon carcinoma cells HT-29 by sulforaphane is associated with induction of p21CIP1 and inhibition of expression of cyclin D1 . Cancer Chemother Pharmacol , 57 : 317 – 327 .
  • Suppipat , K , Park , C S , Shen , Y , Zhu , X and Lacorazza , H D . 2012 . Sulforaphane induces cell cycle arrest and apoptosis in acute lymphoblastic leukemia cells . PLoS One , 7 : e51251
  • Matsui , T A , Murata , H , Sakabe , T , Sowa , Y Horie , N . 2007 . Sulforaphane induces cell cycle arrest and apoptosis in murine osteosarcoma cells in vitro and inhibits tumor growth in vivo . Oncol Rep , 18 : 1263 – 1268 .
  • Chiao , J W , Chung , F L , Kancherla , R , Ahmed , T Mittelman , A . 2002 . Sulforaphane and its metabolite mediate growth arrest and apoptosis in human prostate cancer cells . Int J Oncol , 20 : 631 – 636 .
  • Fimognari , C , Berti , F , Iori , R , Cantelli-Forti , G and Hrelia , P . 2005 . Micronucleus formation and induction of apoptosis by different isothiocyanates and a mixture of isothiocyanates in human lymphocyte cultures . Mutat Res-Gen Tox En , 582 : 1 – 10 .
  • Fimognari , C , Berti , F , Cantelli-Forti , G and Hrelia , P . 2005 . Effect of sulforaphane on micronucleus induction in cultured human lymphocytes by four different mutagens . Environ Mol Mutagen , 46 : 260 – 267 .
  • Tope , A M and Rogers , P F . 2009 . Evaluation of protective effects of sulforaphane on DNA damage caused by exposure to low levels of pesticide mixture using comet assay . J Environ Sci Heal B , 44 : 657 – 662 .
  • Barcelo , S , Gardiner , J M , Gescher , A and Chipman , J K . 1996 . CYP2E1-mediated mechanism of anti-genotoxicity of the broccoli constituent sulforaphane . Carcinogenesis , 17 : 277 – 282 .
  • Gross-Steinmeyer , K , Stapleton , P L , Tracy , J H , Bammler , T K Strom , S C . 2010 . Sulforaphane- and phenethyl isothiocyanate-induced inhibition of aflatoxin B1-mediated genotoxicity in human hepatocytes: role of GSTM1 genotype and CYP3A4 gene expression . Toxicol Sci , 116 : 422 – 432 .
  • Bonnesen , C , Eggleston , I M and Hayes , J D . 2001 . Dietary indoles and isothiocyanates that are generated from cruciferous vegetables can both stimulate apoptosis and confer protection against DNA damage in human colon cell lines . Cancer Res , 61 : 6120 – 6130 .
  • Rudolf , E and Cervinka , M . 2011 . Sulforaphane induces cytotoxicity and lysosome- and mitochondria-dependent cell death in colon cancer cells with deleted p53 . Toxicol in Vitro , 25 : 1302 – 1309 .
  • Cho , S D , Li , G , Hu , H , Jiang , C Kang , K S . 2005 . Involvement of c-Jun N-terminal kinase in G2/M arrest and caspase-mediated apoptosis induced by sulforaphane in DU145 prostate cancer cells . Nutr Cancer , 52 : 213 – 224 .
  • Rudolf , E , Andelova , H and Cervinka , M . 2009 . Activation of several concurrent proapoptic pathways by sulforaphane in human colon cancer cells SW620 . Food Chem Toxicol , 47 : 2366 – 2373 .
  • Sekine-Suzuki , E , Yu , D , Kubota , N , Okayasu , R and Anzai , K . 2008 . Sulforaphane induces DNA double strand breaks predominantly repaired by homologous recombination pathway in human cancer cells . Biochem Bioph Res Co , 377 : 341 – 345 .
  • Sestili , P , Paolillo , M , Lenzi , M , Colombo , E Vallorani , L . 2010 . Sulforaphane induces DNA single strand breaks in cultured human cells . Mutat Res-Fund Mol M , 689 : 65 – 73 .
  • Twentyman , P R and Luscombe , M . 1987 . A study of some variables in a tetrazolium dye (MTT) based assay for cell growth and chemosensitivity . Br J Cancer , 56 : 279 – 285 . 1987
  • Misra , R K and Easton , M D . 1999 . Comment on analyzing flow cytometric data for comparison of mean values of the coefficient of variation of the G1 peak . Cytometry , 36 : 112 – 116 .
  • Rozen , S and Skaletsky , H . 2000 . Primer3 on the WWW for general users and for biologist programmers . Methods Mol Biol , 132 : 365 – 386 .
  • Zhao , S and Fernald , R D . 2005 . Comprehensive algorithm for quantitative real-time polymerase chain reaction . J Comput Biol , 12 : 1047 – 1064 .
  • Pfaffl , M W . 2001 . A new mathematical model for relative quantification in real-time RT-PCR . Nucleic Acids Res , 29 : e45
  • Singh , N P , McCoy , M T , Tice , R R and Schneider , E L . 1988 . A simple technique for quantitation of low levels of DNA damage in individual cells . Exp Cell Res , 175 : 184 – 191 .
  • Tice , R R , Agurell , E , Anderson , D , Burlinson , B Hartmann , A . 2000 . Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing . Environ Mol Mutagen , 35 : 206 – 221 .
  • Fenech , M . 2007 . Cytokinesis-block micronucleus cytome assay . Nat Protoc , 2 : 1084 – 1104 .
  • Fimognari , C , Nusse , M , Cesari , R , Iori , R Cantelli-Forti , G . 2002 . Growth inhibition, cell-cycle arrest and apoptosis in human T-cell leukemia by the isothiocyanate sulforaphane . Carcinogenesis , 23 : 581 – 586 .
  • Song , M Y , Kim , E K , Moon , W S , Park , J W Kim , H J . 2009 . Sulforaphane protects against cytokine- and streptozotocin-induced beta-cell damage by suppressing the NF-kappaB pathway . Toxicol Appl Pharmacol , 235 : 57 – 67 .
  • Matsui , T A , Sowa , Y , Yoshida , T , Murata , H Horinaka , M . 2006 . Sulforaphane enhances TRAIL-induced apoptosis through the induction of DR5 expression in human osteosarcoma cells . Carcinogenesis , 27 : 1768 – 1777 .
  • Sawai , Y , Murata , H , Horii , M , Koto , K Matsui , T . 2013 . Effectiveness of sulforaphane as a radiosensitizer for murine osteosarcoma cells . Oncology Reports , 29 : 941 – 945 .
  • Boutros , R , Lobjois , V and Ducommun , B . 2007 . CDC25 phosphatases in cancer cells: key players? Good targets? . Nat Rev Cancer , 7 : 495 – 507 .
  • Medema , R H and Macurek , L . Checkpoint control and cancer . Oncogene , 31 2601 – 2613 . 2012.
  • Jackson , S J and Singletary , K W . 2004 . Sulforaphane inhibits human MCF-7 mammary cancer cell mitotic progression and tubulin polymerization . J Nutr , 134 : 2229 – 2236 .
  • Jackson , S J and Singletary , K W . 2004 . Sulforaphane: a naturally occurring mammary carcinoma mitotic inhibitor, which disrupts tubulin polymerization . Carcinogenesis , 25 : 219 – 227 .
  • Jackson , S J , Singletary , K W and Venema , R C . 2007 . Sulforaphane suppresses angiogenesis and disrupts endothelial mitotic progression and microtubule polymerization . Vascul Pharmacol , 46 : 77 – 84 .
  • Azarenko , O , Okouneva , T , Singletary , K W , Jordan , M A and Wilson , L . 2008 . Suppression of microtubule dynamic instability and turnover in MCF7 breast cancer cells by sulforaphane . Carcinogenesis , 29 : 2360 – 2368 .
  • Mi , L , Xiao , Z , Hood , B L , Dakshanamurthy , S Wang , X . 2008 . Covalent binding to tubulin by isothiocyanates. A mechanism of cell growth arrest and apoptosis . J Biol Chem , 283 : 22136 – 22146 .
  • Rajendran , P , Delage , B , Dashwood , W M , Yu , T W Wuth , B . Histone deacetylase turnover and recovery in sulforaphane-treated colon cancer cells: competing actions of 14–3–3 and Pin1 in HDAC3/SMRT corepressor complex dissociation/reassembly . Mol Cancer , 10 68 2011.
  • Mi , L , Hood , B L , Stewart , N A , Xiao , Z Govind , S . 2011 . Identification of potential protein targets of isothiocyanates by proteomics . Chem Res Toxicol , 24 : 1735 – 1743 .
  • Fenech , M , Kirsch-Volders , M , Natarajan , A T , Surralles , J Crott , J W . 2011 . Molecular mechanisms of micronucleus, nucleoplasmic bridge and nuclear bud formation in mammalian and human cells . Mutagenesis , 26 : 125 – 132 .
  • Al-Romaih , K , Bayani , J , Vorobyova , J , Karaskova , J Park , P C . 2003 . Chromosomal instability in osteosarcoma and its association with centrosome abnormalities . Cancer Genet Cytogenet , 144 : 91 – 99 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.