The Effect of Silencing HIF-1α Gene in BxPC-3 Cell Line on Glycolysis-Related Gene Expression, Cell Growth, Invasion, and Apoptosis

REFERENCES

• Rodriguez-Enriquez S, Carreno-Fuentes L, Gallardo-Perez JC, et al.: Oxidative phosphorylation is impaired by prolonged hypoxia in breast and possibly in cervix carcinoma. Int J Biochem Cell Biol 42, 1744–1751, 2010.
   PubMed Web of Science ®Google Scholar
• Erickson K, Braun RD, Yu D, Lanzen J, and Wilson D: Effect of longitudinal oxygen gradients on effectiveness of manipulation of tumor oxygenation. Cancer Res 63, 4705–4712, 2003.
   PubMed Web of Science ®Google Scholar
• Horan AD and Koch CJ: The K(m) for radiosensitization of human tumor cells by oxygen is much greater than 3 mmHg and is further increased by elevated levels of cysteine. Radiat Res 156, 388–398, 2001.
   PubMed Web of Science ®Google Scholar
• Graeber TG, Osmanian C, Jacks T, Housman DE, and Koch CJ: Hypoxia-mediated selection of cells with diminished apoptotic potential in solid tumours. Nature 379(6560), 88–91, 1996.
   PubMed Web of Science ®Google Scholar
• Bristow RG and Hill RP: Hypoxia and metabolism. Hypoxia, DNA repair and genetic instability. Nat Rev Cancer 8, 180–192, 2008.
   PubMed Web of Science ®Google Scholar
• Kim JW and Dang CV: Cancer's molecular sweet tooth and the Warburg effect. Cancer Res 66, 8927–8930, 2006.
   PubMed Web of Science ®Google Scholar
• Warburg O: On the origin of cancer cells. Science 123(3191), 309–314, 1956.
   PubMed Web of Science ®Google Scholar
• Ferreira LM: Cancer metabolism: the Warburg effect today. Exp Mol Pathol 89, 372–380, 2010.
   PubMed Web of Science ®Google Scholar
• Kelly C, Smallbone K, and Brady M: Tumour glycolysis: the many faces of HIF. J Theor Biol 254, 508–513, 2008.
   PubMed Web of Science ®Google Scholar
• Wang GL, Jiang BH, Rue EA, and Semenza GL: Hypoxia-inducible factor 1 is a basic-helix-loop-helix-PAS heterodimer regulated by cellular O2 tension. Proc Natl Acad Sci U S A 92, 5510–5514, 1995.
   PubMed Web of Science ®Google Scholar
• Huang LE, Gu J, Schau M, and Bunn HF: Regulation of hypoxia-inducible factor 1alpha is mediated by an O2-dependent degradation domain via the ubiquitin-proteasome pathway. Proc Natl Acad Sci U S A 95, 7987–7992, 1998.
   PubMed Web of Science ®Google Scholar
• Kaelin WG Jr and Ratcliffe PJ: Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol Cell 30, 393–402, 2008.
   PubMed Web of Science ®Google Scholar
• Guzy RD and Schumacker PT: Oxygen sensing by mitochondria at complex III: the paradox of increased reactive oxygen species during hypoxia. Exp Physiol 91, 807–819, 2006.
   PubMed Web of Science ®Google Scholar
• Kim JW, Tchernyshyov I, Semenza GL, and Dang CV: HIF-1-mediated expression of pyruvate dehydrogenase kinase: a metabolic switch required for cellular adaptation to hypoxia. Cell Metab 3, 177–185, 2006.
   PubMed Web of Science ®Google Scholar
• Bonnet D and Dick JE: Human acute myeloid leukemia is organized as a hierarchy that originates from a primitive hematopoietic cell. Nat Med 3, 730–737, 1997.
   PubMed Web of Science ®Google Scholar
• Nakashima RA, Paggi MG, and Pedersen PL: Contributions of glycolysis and oxidative phosphorylation to adenosine 5′-triphosphate production in AS-30D hepatoma cells. Cancer Res 44, 5702–5706, 1984.
   PubMed Web of Science ®Google Scholar
• Nascimento JM, Shi LZ, Tam J, Chandsawangbhuwana C, and Durrant B: Comparison of glycolysis and oxidative phosphorylation as energy sources for mammalian sperm motility, using the combination of fluorescence imaging, laser tweezers, and real-time automated tracking and trapping. J Cell Physiol 217, 745–751, 2008.
   PubMed Web of Science ®Google Scholar
• Pfeiffer T, Schuster S, and Bonhoeffer S: Cooperation and competition in the evolution of ATP-producing pathways. Science 292(5516), 504–507, 2001.
   PubMed Web of Science ®Google Scholar
• Hanze J, Eul BG, Savai R, Krick S, and Goyal P: RNA interference for HIF-1alpha inhibits its downstream signalling and affects cellular proliferation. Biochem Biophys Res Commun 312, 571–577, 2003.
   PubMed Web of Science ®Google Scholar
• Li J, Shi M, Cao Y, Yuan W, and Pang T: Knockdown of hypoxia-inducible factor-1alpha in breast carcinoma MCF-7 cells results in reduced tumor growth and increased sensitivity to methotrexate. Biochem Biophys Res Commun 342, 1341–1351, 2006.
   PubMed Web of Science ®Google Scholar
• Gibson UE, Heid CA, and Williams PM: A novel method for real time quantitative RT-PCR. Genome Res 6, 995–1001, 1996.
   PubMed Web of Science ®Google Scholar
• Haschke-Becher E, Baumgartner M, and Bachmann C: Assay of D-lactate in urine of infants and children with reference values taking into account data below detection limit. Clin Chim Acta 298(1–2), 99–109, 2000.
   PubMed Web of Science ®Google Scholar
• Talasniemi JP, Pennanen S, Savolainen H, Niskanen L, and Liesivuori J. Analytical investigation: assay of D-lactate in diabetic plasma and urine. Clin Biochem 41, 1099–1103, 2008.
   PubMed Web of Science ®Google Scholar
• Chen YY, Chiang SY, Lin JG, Ma YS, and Liao CL: Emodin, aloe-emodin and rhein inhibit migration and invasion in human tongue cancer SCC-4 cells through the inhibition of gene expression of matrix metalloproteinase-9. Int J Oncol 36, 1113–1120, 2010.
   PubMed Web of Science ®Google Scholar
• Liao CL, Lai KC, Huang AC, Yang JS, and Lin JJ: Gallic acid inhibits migration and invasion in human osteosarcoma U-2 OS cells through suppressing the matrix metalloproteinase-2/-9, protein kinase B (PKB) and PKC signaling pathways. Food Chem Toxicol 50, 1734–1740, 2012.
   PubMed Web of Science ®Google Scholar
• Brown JM and Giaccia AJ: The unique physiology of solid tumors: opportunities (and problems) for cancer therapy. Cancer Res 58, 1408–1416, 1998.
   PubMed Web of Science ®Google Scholar
• Semenza GL, Roth PH, Fang HM, and Wang GL: Transcriptional regulation of genes encoding glycolytic enzymes by hypoxia-inducible factor 1. J Biol Chem. 269, 23757–23763, 1994.
   PubMed Web of Science ®Google Scholar
• Papandreou I, Cairns RA, Fontana L, Lim AL, and Denko NC: HIF-1 mediates adaptation to hypoxia by actively downregulating mitochondrial oxygen consumption. Cell Metab 3, 187–197, 2006.
   PubMed Web of Science ®Google Scholar
• Lu CW, Lin SC, Chen KF, Lai YY, and Tsai SJ: Induction of pyruvate dehydrogenase kinase-3 by hypoxia-inducible factor-1 promotes metabolic switch and drug resistance. J Biol Chem 283, 28106–28114, 2008.
   PubMed Web of Science ®Google Scholar
• Marin-Hernandez A, Gallardo-Perez JC, Ralph SJ, Rodriguez-Enriquez S, and Moreno-Sanchez R: HIF-1alpha modulates energy metabolism in cancer cells by inducing over-expression of specific glycolytic isoforms. Mini Rev Med Chem 9, 1084–1101, 2009.
   PubMed Web of Science ®Google Scholar
• McFate T, Mohyeldin A, Lu H, et al.: Pyruvate dehydrogenase complex activity controls metabolic and malignant phenotype in cancer cells. J Biol Chem 283, 22700–22708, 2008.
   PubMed Web of Science ®Google Scholar
• Lu H, Forbes RA, and Verma A: Hypoxia-inducible factor 1 activation by aerobic glycolysis implicates the Warburg effect in carcinogenesis. J Biol Chem 277, 23111–23115, 2002.
   PubMed Web of Science ®Google Scholar
• Koukourakis MI, Giatromanolaki A, Sivridis E, Gatter KC, and Harris AL: Pyruvate dehydrogenase and pyruvate dehydrogenase kinase expression in non small cell lung cancer and tumor-associated stroma. Neoplasia 7(1), 1–6, 2005.
   PubMed Web of Science ®Google Scholar
• Zhang Y, Zhang X, Wang X, Gan L, and Yu G: Inhibition of LDH-A by lentivirus-mediated small interfering RNA suppresses intestinal-type gastric cancer tumorigenicity through the downregulation of Oct4. Cancer Lett. 321(1), 45–54, 2012.
   PubMed Web of Science ®Google Scholar
• Dang CV and Semenza GL: Oncogenic alterations of metabolism. Trends Biochem Sci 24(2), 68–72, 1999.
   PubMed Web of Science ®Google Scholar
• Semenza GL: HIF-1: mediator of physiological and pathophysiological responses to hypoxia. J Appl Physiol 88, 1474–1480, 2000.
   PubMed Web of Science ®Google Scholar
• Semenza GL: HIF-1 and tumor progression: pathophysiology and therapeutics. Trends Mol Med 8(4 Suppl), S62–S67, 2002.
   PubMed Web of Science ®Google Scholar
• Ramanathan A, Wang C, and Schreiber SL: Perturbational profiling of a cell-line model of tumorigenesis by using metabolic measurements. Proc Natl Acad Sci U S A 102, 5992–5997, 2005.
   PubMed Web of Science ®Google Scholar
• Kondoh H, Lleonart ME, Gil J, Wang J, and Degan P: Glycolytic enzymes can modulate cellular life span. Cancer Res 65(1), 177–185, 2005.
   PubMed Web of Science ®Google Scholar
• Zhong H, De Marzo AM, Laughner E, Lim M, and Hilton DA: Overexpression of hypoxia-inducible factor 1alpha in common human cancers and their metastases. Cancer Res. 59, 5830–5835, 1999.
   PubMed Web of Science ®Google Scholar
• Wei H, Li F, Fu P, and Liu X: Effects of the silencing of hypoxia-inducible factor-1 alpha on metastasis of pancreatic cancer. European Review for Medical and Pharmacological Science 17, 436–446, 2013.
   PubMed Web of Science ®Google Scholar
• Akakura N, Kobayashi M, Horiuchi I, Suzuki A, and Wang J: Constitutive expression of hypoxia-inducible factor-1alpha renders pancreatic cancer cells resistant to apoptosis induced by hypoxia and nutrient deprivation. Cancer Res 61, 6548–6554, 2001.
   PubMed Web of Science ®Google Scholar
• Chen J, Zhao S, Nakada K, Kuge Y, and Tamaki N: Dominant-negative hypoxia-inducible factor-1 alpha reduces tumorigenicity of pancreatic cancer cells through the suppression of glucose metabolism. Am J Pathol 162, 1283–1291, 2003.
   PubMed Web of Science ®Google Scholar
• Li L, Lin X, Staver M, Shoemaker A, and Semizarov D: Evaluating hypoxia-inducible factor-1alpha as a cancer therapeutic target via inducible RNA interference in vivo. Cancer Res 65, 7249–7258, 2005.
   PubMed Web of Science ®Google Scholar