Antimicrobial, Antioxidant, and Anti-Tumor Activities of Sargassum linearifolium and Cystoseira crinita from Egyptian Mediterranean Coast

References

• Ferlay J, Soerjomataram I, Ervik M, Dikshit R, Eser S, et al. GLOBOCAN 2012 v1.0, Cancer Incidence and Mortality Worldwide: IARC CancerBase. No. p. 11. [Internet]. Lyon, France: International Agency for Research on Cancer, 2013.
   Google Scholar
• Anand P, Kunnumakara AB, Sundaram C, Harikumar KB, Tharakan ST, Lai OS, Sung B, Aggarwal BB. Cancer is a preventable disease that requires major lifestyle changes. Pharm Res. 2008;25(9):2097–116. doi:10.1007/s11095-008-9661-9
   PubMed Web of Science ®Google Scholar
• Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clin. 2018;68(6):394–424. doi:10.3322/caac.21492
   PubMed Web of Science ®Google Scholar
• Perez-Herrero E, Fernandez-Medarde A. Advanced targeted therapies in cancer: Drug nanocarriers, the future of chemotherapy. Eur J Pharm Biopharm. 2015;93:52–79.
   PubMed Web of Science ®Google Scholar
• Abourriche A, Charrouf M, Berrada M, Bennamara A, Chaib N, Francisco C. Antimicrobial activities and cytotoxicity of the brown algae Cystoseira tamariscifolia. Fitoterapia. 1999;70(6):611–4. doi:10.1016/S0367-326X(99)00088-X
   Google Scholar
• Zubia M, Fabre MS, Kerjean V, Lann KL, Stiger-Pouvreau V, Fauchon M, Deslandes E. Antioxidant and antitumoural activities of some Phaeophyta from Brittany coasts. Food Chem. 2009;116(3):693–701. doi:10.1016/j.foodchem.2009.03.025
   Web of Science ®Google Scholar
• Correia-da-Silva M, Sousa E, Pinto MMM, Kijjoa A. Anticancer and cancer preventive compounds from edible marine organisms. Semin Cancer Biol. 2017;46:55–64. doi:10.1016/j.semcancer.2017.03.011
   PubMed Web of Science ®Google Scholar
• Alves C, Silva J, Pinteus S, Gaspar H, Alpoim MC, Botana LM, Pedrosa R. From marine origin to therapeutics: the antitumor potential of marine algae-derived compounds. Front Pharmacol. 2018;9:777doi:10.3389/fphar.2018.00777
   PubMed Web of Science ®Google Scholar
• Ercolano G, De Cicco P, Ianaro A. New drugs from the sea: pro-apoptotic activity of sponges and algae derived compounds. Mar Drugs. 2019;17(1):31. doi:10.3390/md17010031
   PubMed Web of Science ®Google Scholar
• Rodrigues D, Freitas AC, Pereira L, Rocha-Santos TAP, Vasconcelos MW, Roriz M, Rodríguez-Alcalá LM, Gomes AMP, Duarte AC. Chemical composition of red, brown and green macroalgae from Buarcos bay in Central West Coast of Portugal. Food Chem. 2015;183:197–207. doi:10.1016/j.foodchem.2015.03.057
   PubMed Web of Science ®Google Scholar
• Ismail GA. Biochemical composition of some Egyptian seaweeds with potent nutritive and antioxidant properties. Food Sci Technol. 2017;37(2):294–302. doi:10.1590/1678-457x.20316
   Web of Science ®Google Scholar
• Wells ML, Potin P, Craigie JS, Raven JA, Merchant SS, Helliwell KE, Smith AG, Camire ME, Brawley SH. Algae as nutritional and functional food sources: revisiting our understanding. J Appl Phycol. 2017;29(2):949–82. doi:10.1007/s10811-016-0974-5
   PubMed Web of Science ®Google Scholar
• Pérez MJ, Falque E, Dominguez H. Antimicrobial action of compounds from marine seaweed. Mar Drugs. 2016;14(3):52. doi:10.3390/md14030052
   PubMed Web of Science ®Google Scholar
• Vizetto-Duarte C, Pereira H, Bruno de Sousa C, Pilar Rauter A, Albericio F, Custódio L, Barreira L, Varela J. Fatty acid profile of different species of algae of the Cystoseira genus: a nutraceutical perspective. Nat Prod Res. 2015;29(13):1264–70. doi:10.1080/14786419.2014.992343
   PubMed Web of Science ®Google Scholar
• Rocha DHA, Seca AML, Pinto D. Seaweed secondary metabolites In Vitro and In Vivo anticancer activity. Mar Drugs. 2018;16(11):410. doi:10.3390/md16110410
   PubMed Web of Science ®Google Scholar
• Namvar F, Baharara J, Mahdi AA. Antioxidant and anticancer activities of selected persian gulf algae. Ind J Clin Biochem. 2014;29(1):13–20. doi:10.1007/s12291-013-0313-4
   PubMedGoogle Scholar
• Mayer AMS, Rodriguez AD, Taglialatela-Scafati O, Fusetani N. Marine Pharmacology in 2012–2013: Marine compounds with antibacterial, antidiabetic, antifungal, anti-Inflammatory, antiprotozoal, antituberculosis, and antiviral activities; Affecting the immune and nervous systems, and other miscellaneous mechanisms of action. Mar Drugs. 2017;15(9):273. doi:10.3390/md15090273
   PubMed Web of Science ®Google Scholar
• Khalid S, Abbas M, Saeed F, Bader-Ul-Ain H, and, Suleria H. Therapeutic potential of seaweed bioactive compounds. In: Maiti S editor. Seaweed biomaterials. London: IntechOpen, 2018. p. 7–25.
   Google Scholar
• Guiry MD, Guiry GM. AlgaeBase. World-wide electronic publication. Galway: National University of Ireland, 2018. http://www.algaebase.org.
   Google Scholar
• Pinteus S, Lemos M, Silva J, Alves C, Neugebauer A, Freitas R, Duarte A, Pedrosa R. An insight into Sargassum muticum cytoprotective mechanisms against oxidative stress on a human cell In Vitro model. Mar Drugs. 2017;15(11):353. doi:10.3390/md15110353
   PubMed Web of Science ®Google Scholar
• Shreadah MA, El Moneam NMA, Al-Assar SA, Nabil-Adam A. Phytochemical and pharmacological screening of Sargassium vulgare from Suez Canal. Food Sci Biotechnol. 2018;27(4):963–79. doi:10.1007/s10068-018-0323-3
   PubMed Web of Science ®Google Scholar
• Lim S, Choi A-H, Kwon M, Joung E-J, Shin T, Lee S-G, Kim N-G, Kim H-R. Evaluation of antioxidant activities of various solvent extract from Sargassum serratifolium and its major antioxidant components. Food Chem. 2019;278:178–84. doi:10.1016/j.foodchem.2018.11.058
   PubMed Web of Science ®Google Scholar
• Moubayed NM, Al Houri HJ, Al Khulaifi MM, Al Farraj DA. Antimicrobial, antioxidant properties and chemical composition of seaweeds collected from Saudi Arabia (Red Sea and Arabian Gulf). Saudi J Biol Sci. 2017;24(1):162–9. doi:10.1016/j.sjbs.2016.05.018
   PubMed Web of Science ®Google Scholar
• Li Y, Sun S, Pu X, Yang Y, Zhu F, Zhang S, Xu N. Evaluation of antimicrobial activities of seaweed resources from Zhejiang Coast, China. Sustainability. 2018;10(7):2158. doi:10.3390/su10072158
   Web of Science ®Google Scholar
• Palanisamy S, Vinosha M, Rajasekar P, Anjali R, Sathiyaraj G, Marudhupandi T, Selvam S, Prabhu NM, You S. Antibacterial efficacy of a fucoidan fraction (Fu-F2) extracted from Sargassum polycystum. Int J Biol Macromol. 2019;125:485–95. doi:10.1016/j.ijbiomac.2018.12.070
   PubMed Web of Science ®Google Scholar
• Patra S, Muthuraman M, Prabhu A, Priyadharshini R, Parthiban S. Evaluation of antitumor and antioxidant activity of Sargassum tenerrimum against Ehrlich ascites carcinoma in mice. Asian Pac J Cancer Prev. 2015;16(3):915–21. doi:10.7314/APJCP.2015.16.3.915
   PubMedGoogle Scholar
• Vaseghi G, Sharifi M, Dana N, Ghasemi A, Yegdaneh A. Cytotoxicity of Sargassum angustifolium partitions against breast and cervical cancer cell lines. Adv Biomed Res. 2018;7(1):43. doi:10.4103/abr.abr_259_16
   PubMedGoogle Scholar
• Kim ME, Jung YC, Jung I, Lee H-W, Youn H-Y, Lee JS. Anti-inflammatory effects of ethanolic extract from Sargassum horneri (Turner) C. Agardh on lipopolysaccharide-stimulated macrophage activation via NF-kappaB pathway regulation. Immunol Invest. 2015;44(2):137–46. doi:10.3109/08820139.2014.942459
   PubMed Web of Science ®Google Scholar
• Oh S-J, Joung E-J, Kwon M-S, Lee B, Utsuki T, Oh C-W, Kim H-R. Anti-Inflammatory effect of ethanolic extract of Sargassum serratifolium in lipopolysaccharide-stimulated BV2 microglial cells. J Med Food. 2016;19(11):1023–31. doi:10.1089/jmf.2016.3732
   PubMed Web of Science ®Google Scholar
• Jeon H, Yoon WJ, Ham YM, Yoon SA, Kang SC. Anti-Arthritis effect through the anti-Inflammatory effect of Sargassum muticum extract in collagen-induced arthritic (CIA) mice. Molecules. 2019;24(2):276. doi:10.3390/molecules24020276
   PubMed Web of Science ®Google Scholar
• Kim SN, Lee W, Bae GU, Kim YK. Anti-diabetic and hypolipidemic effects of Sargassum yezoense in db/db mice. Biochem Biophys Res Commun. 2012;424(4):675–80. doi:10.1016/j.bbrc.2012.07.005
   PubMed Web of Science ®Google Scholar
• Kolsi RBA, Salah HB, Jardak N, Chaaben R, Jribi I, Feki AE, Rebai T, Jamoussi K, Allouche N, Blecker C, et al. Sulphated polysaccharide isolated from Sargassum vulgare: Characterization and hypolipidemic effects. Carbohydr Polym. 2017;170:148–59.: doi:10.1016/j.carbpol.2017.04.083
   PubMed Web of Science ®Google Scholar
• Akbarzadeh S, Gholampour H, Farzadinia P, Daneshi A, Ramavandi B, Moazzeni A, Keshavarz M, Bargahi A. Anti-diabetic effects of Sargassum oligocystum on Streptozotocin-induced diabetic rat. Iran J Basic Med Sci. 2018;21(3):342–6. doi:10.22038/IJBMS.2018.25654.6329
   PubMed Web of Science ®Google Scholar
• Mhadhebi L, Mhadhebi A, Robert J, Bouraoui A. Antioxidant, anti-inflammatory and antiproliferative effects of aqueous extracts of three Mediterranean brown seaweeds of the genus Cystoseira. Iran J Pharm Res. 2014;13(1):207–20.
   PubMed Web of Science ®Google Scholar
• Vizetto-Duarte C, Custódio L, Acosta G, Lago JHG, Morais TR, Bruno de Sousa C, Gangadhar KN, Rodrigues MJ, Pereira H, Lima RT, et al. Can macroalgae provide promising anti-tumoral compounds? A closer look at Cystoseira tamariscifolia as a source for antioxidant and anti-hepatocarcinoma compounds. PeerJ. 2016;4:e1704.: doi:10.7717/peerj.1704
   PubMed Web of Science ®Google Scholar
• Yegdaneh A, Ghannadi A, Dayani L. Chemical constituents and biological activities of two Iranian Cystoseira species. Res Pharma Sci. 2016;11(4):311–7. doi:10.4103/1735-5362.189307
   PubMed Web of Science ®Google Scholar
• Sellimi S, Benslima A, Barragan-Montero V, Hajji M, Nasri M. Polyphenolic-protein-polysaccharide ternary conjugates from Cystoseira barbata Tunisian seaweed as potential biopreservatives: chemical, antioxidant and antimicrobial properties. Int J Biol Macromol. 2017;105:1375–83. doi:10.1016/j.ijbiomac.2017.08.007
   PubMed Web of Science ®Google Scholar
• Abdelhamid A, Jouini M, Bel Haj Amor H, Mzoughi Z, Dridi M, Ben Said R, Bouraoui A. Phytochemical analysis and evaluation of the antioxidant, anti-Inflammatory, and antinociceptive potential of phlorotannin-rich fractions from three Mediterranean brown seaweeds. Mar Biotechnol. 2018;20(1):60–74. doi:10.1007/s10126-017-9787-z
   PubMed Web of Science ®Google Scholar
• Güner A, Köksal Ç, Erel ŞB, Kayalar H, Nalbantsoy A, Sukatar A, Karabay Yavaşoğlu NÜ. Antimicrobial and antioxidant activities with acute toxicity, cytotoxicity and mutagenicity of Cystoseira compressa (Esper) Gerloff & Nizamuddin from the coast of Urla (Izmir, Turkey). Cytotechnol. 2015;67(1):135–43. doi:10.1007/s10616-013-9668-x
   PubMed Web of Science ®Google Scholar
• Kosanic M, Rankovic B, Stanojkovic T. Biological potential of marine macroalgae of the genus Cystoseira. Acta Biol Hung. 2015;66:374–84.
   PubMed Web of Science ®Google Scholar
• Coll M, Piroddi C, Steenbeek J, Kaschner K, Ben Rais Lasram F, Aguzzi J, Ballesteros E, Bianchi CN, Corbera J, Dailianis T, et al. The biodiversity of the Mediterranean Sea: estimates, patterns, and threats. PLoS One. 2010;5(8):e11842: doi:10.1371/journal.pone.0011842
   PubMed Web of Science ®Google Scholar
• Gheda SF, El-Adawi HI, Nm E-D. Antiviral profile of brown and red seaweed polysaccharides against hepatitis C virus. Iran J Pharm Res. 2016;15:483–91.
   PubMed Web of Science ®Google Scholar
• Mohy El-Din SM, El-Ahwany AMD. Bioactivity and phytochemical constituents of marine red seaweeds (Jania rubens, Corallina mediterranea and Pterocladia capillacea). J Taibah Uni Sci. 2016;10(4):471–84. doi:10.1016/j.jtusci.2015.06.004
   Web of Science ®Google Scholar
• Ismail MM, El Zokm GM, El-Sayed AAM. Variation in biochemical constituents and master elements in common seaweeds from Alexandria Coast, Egypt, with special reference to their antioxidant activity and potential food uses: prospective equations. Environ Monit Assess. 2017;189(12):648. doi:10.1007/s10661-017-6366-8
   PubMed Web of Science ®Google Scholar
• Aleem AA. Marine algae of Alexandria, Egypt. In: Aleem AA editor. Egyptian Books House, Egypt. Faculty of science. Alexandria: University of Alexandria, , 1993. 138, pp. 1–55.
   Google Scholar
• Heo SJ, Cha SH, Lee KW, Jeon YJ. Antioxidant activities of red algae from Jeju Island. Algae. 2006;21(1):149–56. doi:10.4490/ALGAE.2006.21.1.149
   Google Scholar
• Singleton VL, Orthofer R, Rm L-R. Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. Methods Enzymol. 1999;299:152–78.
   Web of Science ®Google Scholar
• Andrews JM. Determination of minimum inhibitory concentrations. J Antimicrob Chemother. 2001;48(suppl_1):5–16. doi:10.1093/jac/48.suppl_1.5
   PubMed Web of Science ®Google Scholar
• Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685–8. doi:10.5897/AJB2005.000-3127
   Web of Science ®Google Scholar
• Burits M, Bucar F. Antioxidant activity of Nigella sativa essential oil. Phytother Res. 2000;14(5):323–8. doi:10.1002/1099-1573(200008)14:5<323::AID-PTR621>3.0.CO;2-Q
   PubMed Web of Science ®Google Scholar
• Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radic Biol Med. 1999;26(9-10):1231–7. doi:10.1016/S0891-5849(98)00315-3
   PubMed Web of Science ®Google Scholar
• Prieto P, Pineda M, Aguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem. 1999;269(2):337–41. doi:10.1006/abio.1999.4019
   PubMed Web of Science ®Google Scholar
• Collee J, Fraser G, Marmion P, and, Simmons A. Mackie and McCartney Practical Medical Microbiology. 4th ed. New York: Churchill Livingstone, 1996.
   Google Scholar
• CLSI. Performance standards for antimicrobial disk susceptibility tests; approved standard. 11th ed. CLSI document M02-A11. vol 32, Wayne, PA: Clinical and Laboratory Standards Institute, 2012, p. 1.
   Google Scholar
• Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods. 2001;25(4):402–8. doi:10.1006/meth.2001.1262
   PubMed Web of Science ®Google Scholar
• Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976;72(1-2):248–54. doi:10.1016/0003-2697(76)90527-3
   PubMed Web of Science ®Google Scholar
• Fadda A, El Defrawy AM. El-Hadidy SA: Synthesis, cytotoxicity evaluation, DFT molecular modeling studies and quantitative structure activity relationship of novel 1,8-naphthyridines. Am J Org Chem. 2012;2:87–96.
   Google Scholar
• Balboa EM, Conde E, Moure A, Falqué E, Domínguez H. In vitro antioxidant properties of crude extracts and compounds from brown algae. Food Chem. 2013;138(2-3):1764–85. doi:10.1016/j.foodchem.2012.11.026
   PubMed Web of Science ®Google Scholar
• Generalić Mekinić I, Skroza D, Šimat V, Hamed I, Čagalj M, Popović Perković Z. Phenolic content of brown algae (pheophyceae) species: extraction, identification, and quantification. Biomolecules. 2019;9(6):244. doi:10.3390/biom9060244
   PubMed Web of Science ®Google Scholar
• Mehdinezhad N, Ghannadi A, Yegdaneh A. Phytochemical and biological evaluation of some Sargassum species from Persian Gulf. Res Pharm Sci. 2016;11(3):243–9.
   PubMed Web of Science ®Google Scholar
• Pinteus S, Silva J, Alves C, Horta A, Fino N, Rodrigues AI, Mendes S, Pedrosa R. Cytoprotective effect of seaweeds with high antioxidant activity from the Peniche coast (Portugal). Food Chem. 2017;218:591–9. doi:10.1016/j.foodchem.2016.09.067
   PubMed Web of Science ®Google Scholar
• Pacheco BS, D, Santos MAZ, Schultze E, Martins RM, Lund RG, et al. Cytotoxic activity of fatty acids from Antarctic macroalgae on the growth of human breast cancer cells. Front Bioengin Biotechnol. 2018;6:185.
   PubMed Web of Science ®Google Scholar
• Dawczynski C, Schubert R, Jahreis G. Amino acids, fatty acids, and dietary fiber in edible seaweed products. Food Chem. 2007;103(3):891–9. doi:10.1016/j.foodchem.2006.09.041
   Web of Science ®Google Scholar
• Das U. Essential fatty acids enhance free radical generation and lipid peroxidation toinduce apoptosis of tumor cells. J Clin Lipidol. 2011;6(4):463–89. doi:10.2217/clp.11.34
   Web of Science ®Google Scholar
• Hidayati JR, Yudiati E, Pringgenies D, Arifin Z, Oktaviyanti DT. Antioxidant activities, total phenolic compound and pigment contents of tropical Sargassum sp. extract, macerated in different solvents polarity. J Kel Trop. 2019;22(1):73–80. doi:10.14710/jkt.v22i1.4404
   Google Scholar
• Jassbi AR, Mohabati M, Eslami S, Sohrabipour J, Miri R. Biological activity and chemical constituents of red and brown algae from the persian gulf. Iran J Pharm Res. 2013;12:339–48.
   PubMed Web of Science ®Google Scholar
• Jaswir I, Tope AHT, Raus RA, Monsur HA, Ramli N. Study on anti-bacterial potentials of some Malaysian brown seaweeds. Food Hydrocoll. 2014;42:275–9. doi:10.1016/j.foodhyd.2014.03.008
   Web of Science ®Google Scholar
• El-Shouny W, Gaafar RM, Ismail GA, Elzanaty MM. Antibacterial activity of some seaweed extracts against multidrug resistant urinary tract bacteria and analysis of their virulence genes. Int J Curr Microbiol App Sci. 2017;6:2569–86.
   Google Scholar
• Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564–82. doi:10.1128/CMR.12.4.564
   PubMed Web of Science ®Google Scholar
• Desbois PA, Smith JV. Antibacterial free fatty acids: activities, mechanisms of action and biotechnological potential. Appl Microbiol Biotechnol. 2010;85(6):1629–42. doi:10.1007/s00253-009-2355-3
   PubMed Web of Science ®Google Scholar
• El Shafay SM, Ali SS, El-Sheekh MM. Antimicrobial activity of some seaweeds species from Red sea, against multidrug resistant bacteria. Egypt J Aquat Res. 2016;42(1):65–74. doi:10.1016/j.ejar.2015.11.006
   Google Scholar
• Namvar F, Mohamad R, Baharara J, Zafar-Balanejad S, Fargahi F, Rahman HS. Antioxidant, antiproliferative, and antiangiogenesis effects of polyphenol-rich seaweed (Sargassum muticum). BioMed Res Int. 2013;2013:1–9. 2013. doi:10.1155/2013/604787
   Web of Science ®Google Scholar
• Singh M, Bhatnagar P, Mishra S, Kumar P, Shukla Y, Gupta KC. PLGA-encapsulated tea polyphenols enhance the chemotherapeutic efficacy of cisplatin against human cancer cells and mice bearing Ehrlich ascites carcinoma. Int J Nanomed. 2015;10:6789–809.
   PubMed Web of Science ®Google Scholar
• Jo EH, Cho SD, Ahn NS, Jung JW, Yang SR. Inhibition of human breast carcinoma by BLC (Sargassum fulvellum) and BLC/HEN Egg in vitro and in vivo. Korean J Vet Res. 2005;45:85–91.
   Google Scholar
• Fan S, Zhang J, Nie W, Zhou W, Jin L, Chen X, Lu J. Antitumor effects of polysaccharide from Sargassum fusiforme against human hepatocellular carcinoma HepG2 cells. Food Chem Toxicol. 2017;102:53–62. doi:10.1016/j.fct.2017.01.020
   PubMed Web of Science ®Google Scholar
• Liu G, Kuang S, Wu S, Jin W, Sun C. A novel polysaccharide from Sargassum integerrimum induces apoptosis in A549 cells and prevents angiogensis in vitro and in vivo. Sci Rep. 2016;6(1):26722. doi:10.1038/srep26722
   PubMed Web of Science ®Google Scholar
• Shao P, Chen X, Sun P. Chemical characterization, antioxidant and antitumor activity of sulfated polysaccharide from Sargassum horneri. Carbohydr Polym. 2014;105:260–9. doi:10.1016/j.carbpol.2014.01.073
   PubMed Web of Science ®Google Scholar
• Silva Costa L, Silva Telles CB, Medeiros Oliveira R, Duarte Barreto Nobre LT, Dantas-Santos N, Barros Gomes Camara R, Santana Santos Pereira Costa M, Almeida-Lima J, Melo-Silveira RF, Lopes Albuquerque IR, et al. Heterofucan from Sargassum filipendula induces apoptosis in HeLa cells. Mar Drugs. 2011;9(4):603–14., : doi:10.3390/md9040603
   PubMed Web of Science ®Google Scholar
• Vizetto-Duarte C, Custódio L, Gangadhar KN, Lago JHG, Dias C, Matos AM, Neng N, Nogueira JMF, Barreira L, Albericio F, et al. Isololiolide, a carotenoid metabolite isolated from the brown alga Cystoseira tamariscifolia, is cytotoxic and able to induce apoptosis in hepatocarcinoma cells through caspase-3 activation, decreased Bcl-2 levels, increased p53 expression and PARP cleavage. Phytomedicine. 2016;23(5):550–7.: doi:10.1016/j.phymed.2016.02.008
   PubMed Web of Science ®Google Scholar
• Islam MA, Kim YS, Jang WJ, Lee SM, Kim HG, Kim SY, Kim JO, Ha YL. A mixture of trans, trans conjugated linoleic acid induces apoptosis in MCF-7 human breast cancer cells with reciprocal expression of Bax and Bcl-2. J Agric Food Chem. 2008;56(14):5970–6. doi:10.1021/jf8004977
   PubMed Web of Science ®Google Scholar
• Koba K, Yanagita T. Health benefits of conjugated linoleic acid (CLA). Obes Res Clin Pract. 2014;8(6):e525–532. doi:10.1016/j.orcp.2013.10.001
   PubMed Web of Science ®Google Scholar
• Lim J-N, Oh J-J, Wang T, Lee J-S, Kim S-H, Kim Y-J, Lee H-G. trans-11 18:1 vaccenic acid (TVA) has a direct anti-carcinogenic effect on MCF-7 human mammary adenocarcinoma cells. Nutrients. 2014;6(2):627–36. doi:10.3390/nu6020627
   PubMed Web of Science ®Google Scholar
• Liu J, Chen B, Yang Y, Wang X. Effect of apoptosis in human mammary cancer (MCF-7) cells induced by cis9, t11-conjugated linoleic acid. Wei Sheng Yan Jiu. 2004;33(1):58–62.
   PubMedGoogle Scholar
• Kim YS, Cerbo RM, Hah CK, Bahn KN, Kim JO, Ha YL. Growth inhibition of osteosarcoma cell MG-63 by a mixture of trans, trans conjugated linoleic acid isomers: possible mechanistic actions. J Food Sci. 2007;73(1):T7–15. doi:10.1111/j.1750-3841.2007.00584.x
   Web of Science ®Google Scholar
• Wang J, Liu X, Zhang X, Liu J, Ye S, Xiao S, Chen H, Wang H. Induction of apoptosis by c9, t11-CLA in human endometrial cancer RL 95-2 cells via ERalpha-mediated pathway. Chem Phys Lipids. 2013;175-176:27–32. doi:10.1016/j.chemphyslip.2013.07.009
   PubMed Web of Science ®Google Scholar
• Lu G, Zhang G, Zheng X, Zeng Y, Xu Z, et al. c9, t11- conjugated linoleic acid induces HCC cell apoptosis and correlation with PPAR-gamma signaling pathway. Am J Trans Res. 2015;7:2752–63.:
   PubMed Web of Science ®Google Scholar
• Janicke RU. MCF-7 breast carcinoma cells do not express caspase-3. Breast Cancer Res Treat. 2009;117:219–21.
   PubMed Web of Science ®Google Scholar
• Wang S, He M, Li L, Liang Z, Zou Z, Tao A. Cell-in-cell death is not restricted by caspase-3 deficiency in MCF-7 cells. J Breast Cancer. 2016;19(3):231–41. doi:10.4048/jbc.2016.19.3.231
   PubMed Web of Science ®Google Scholar
• Carvalho ACP, Sharpe J, Rosenstock TR, Teles AFV, Kowaltowski AJ, Youle RJ, Smaili SS. Bax affects intracellular Ca2+ stores and induces Ca2+ wave propagation. Cell Death Differ. 2004;11(12):1265–76. doi:10.1038/sj.cdd.4401508
   PubMed Web of Science ®Google Scholar
• Finucane DM, Bossy-Wetzel E, Waterhouse NJ, Cotter TG, Green DR. Bax-induced caspase activation and apoptosis via cytochrome c release from mitochondria is inhibitable by Bcl-xL. J Biol Chem. 1999;274(4):2225–33. doi:10.1074/jbc.274.4.2225
   PubMed Web of Science ®Google Scholar
• Xiang J, Chao DT, Korsmeyer SJ. BAX-induced cell death may not require interleukin 1 beta-converting enzyme-like proteases. Proc Natl Acad Sci USA. 1996;93(25):14559–63. doi:10.1073/pnas.93.25.14559
   PubMed Web of Science ®Google Scholar
• Kagawa S, Gu J, Honda T, McDonnell TJ, Swisher SG, et al. Deficiency of caspase-3 in MCF7 cells blocks Bax-mediated nuclear fragmentation but not cell death. Clin Cancer Res. 2001;7:1474–80.:
   PubMed Web of Science ®Google Scholar
• Demaurex N, Distelhorst C. Apoptosis–the calcium connection. Science. 2003;300(5616):65–7. doi:10.1126/science.1083628
   PubMed Web of Science ®Google Scholar
• Pinton P, Giorgi C, Siviero R, Zecchini E, Rizzuto R. Calcium and apoptosis: ER-mitochondria Ca2+ transfer in the control of apoptosis. Oncogene. 2008;27(50):6407–18. doi:10.1038/onc.2008.308
   PubMed Web of Science ®Google Scholar
• Rong Y, Distelhorst CW. Bcl-2 protein family members: versatile regulators of calcium signaling in cell survival and apoptosis. Annu Rev Physiol. 2008;70(1):73–91. doi:10.1146/annurev.physiol.70.021507.105852
   PubMed Web of Science ®Google Scholar
• Ravanan P, Srikumar IF, Talwar P. Autophagy: the spotlight for cellular stress responses. Life Sci. 2017;188:53–67. doi:10.1016/j.lfs.2017.08.029
   PubMed Web of Science ®Google Scholar
• Doherty J, Baehrecke EH. Life, death and autophagy. Nat Cell Biol. 2018;20(10):1110–7. doi:10.1038/s41556-018-0201-5
   PubMed Web of Science ®Google Scholar
• Bialik S, Dasari SK, Kimchi A. Autophagy-dependent cell death - where, how and why a cell eats itself to death. J Cell Sci. 2018;131(18):jcs215152–12. doi:10.1242/jcs.215152
   PubMed Web of Science ®Google Scholar
• Carew JS, Kelly KR, Nawrocki ST. Autophagy as a target for cancer therapy: new developments. Cancer Manag Res. 2012;4:357–65. doi:10.2147/CMAR.S26133
   PubMedGoogle Scholar
• Ozpolat B, Benbrook DM. Targeting autophagy in cancer management - strategies and developments. Cancer Manag Res. 2015;7:291–9. doi:10.2147/CMAR.S34859
   PubMed Web of Science ®Google Scholar
• Yun CW, Lee SH. The roles of autophagy in cancer. IJMS. 2018;19(11):3466. doi:10.3390/ijms19113466
   Web of Science ®Google Scholar
• Yu L, Alva A, Su H, Dutt P, Freundt E, et al. Regulation of an ATG7-beclin 1 program of autophagic cell death by caspase-8. Science. 2004;304(5676):1500–2.: doi:10.1126/science.1096645
   PubMed Web of Science ®Google Scholar
• Pattingre S, Levine B. Bcl-2 inhibition of autophagy: a new route to cancer? Cancer Res. 2006;66(6):2885–8. doi:10.1158/0008-5472.CAN-05-4412
   PubMed Web of Science ®Google Scholar
• Luo S, Rubinsztein DC. Atg5 and Bcl-2 provide novel insights into the interplay between apoptosis and autophagy. Cell Death Differ. 2007;14(7):1247–50. doi:10.1038/sj.cdd.4402149
   PubMed Web of Science ®Google Scholar