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Original Articles

An alternative to acetolysis: application of an enzyme-based method for the palynological preparation of fresh pollen, honey samples and bee capsules

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References

  • Brown CA. 2008. Palynological Techniques, Second Edition. Riding JB, Warny, S. (editors). American Association of Stratigraphic Palynologists Foundation, Dallas, 137 p.
  • Kuss-Wymer CL, Cyr RJ. 1992. Tobacco protoplasts differentiate into elongate cells without net microtubule depolymerization. Protoplasma 168:64–72.
  • Nagata T, Okada K, Takebe I, Matsu C. 1981. Delivery of tobacco mosaic virus RNA into plant protoplasts mediated by reverse-phase evaporation vesicles (liposomes). Mol Gen Genet. 184:161–165.
  • Reitsma T. 1969. Size modifications of recent pollen grains under different treatments. Rev Palaeobot Palyno. 69:23–47.
  • Riding JB, Kyffin-Hughes JE. 2004. A review of the laboratory preparation of palynomorphs with a description of an effective non-acid technique. Revista Brasileira de Paleontologia 7:13–44.
  • Schols P, Es K, D'hondt C, Merckx V, Smets E, Huysmans S. 2004. A new enzyme-based method for the treatment of fragile pollen grains collected from herbarium material. Taxon 53:777–782.
  • Sonobe S. 1990. Cytochalasin B enhances cytokinetic cleavage in miniprotoplasts isolated from cultured tobacco cells. Protoplasma 155:239–242.
  • Van Wichelen J, Camelbeke K, Chaerle P, Goetghebeur P, Huysmans S. 1999. Comparison of different treatments for LM and SEM and systematic value of pollen grains in Cyperaceae. Grana 38:50–58.

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