DIVERSITY AND ABUNDANCE OF POTENTIALLY TOXIC PSEUDO-NITZSCHIA PERAGALLO IN AVEIRO COASTAL LAGOON, PORTUGAL AND DESCRIPTION OF A NEW VARIETY, P. PUNGENS VAR. AVEIRENSIS VAR. NOV.

REFERENCES

• ABRANTES , F. and MOITA , M. T. 1999 . Water column and sediment data on diatoms and coccolithophorids, off Portugal, confirm sediment record of upwelling events . Oceanologica Acta , 22 : 319 – 336 .
   Google Scholar
• AKALLAL , R. , BILLARD , C. , FRESNEL , J. , GIVERNAUD , T. and MOURADI , A. 2002 . Contribution & l'étude du phytoplancton de la côte atlantique marocaine II. Le genre Pseudo-nitzschia (Bacillariophyceae) . Cryptogamie, Algologie , 23 : 187 – 202 .
   Google Scholar
• ALMANDOZ , G. O. , FERRARIO , M. E. , FERREYRA , G. A. , SCHLOSS , I. R. , ESTEVES , J. L. and PAPARAZZO , F. E. 2007 . The genus Pseudo-nitzschia (Bacillariophyceae) in continental shelf waters of Argentina (Southwestern Atlantic Ocean, 38–55°S) . Harmful Algae , 6 : 93 – 103 .
   Web of Science ®Google Scholar
• AMATO , A. , KOOISTRA , W. H. C. F. , GHIRON , J. H. L. , MANN , D. G. , PRÖSHOLD , T. and MONTRESOR , M. 2007 . Reproductive isolation among sympatric cryptic species in marine diatoms . Protist , 158 : 193 – 207 .
   PubMed Web of Science ®Google Scholar
• BARGU , S. , KORAY , T. and LUNDHOLM , N. 2002 . First report of Pseudo-nitzschia calliantha Lundholm, Moestrup & Hasle 2003, a new potentially toxic species from Turkish coasts . E. U. Journal of Fisheries & Aquatic Sciences , 19 : 479 – 483 .
   Google Scholar
• BATES , S. S. 1998 . “ Ecophysiology and metabolism of ASP toxin production ” . In Physiological Ecology of Harmful Algal Blooms , Edited by: Anderson , D. M. , Cembella , A. D. and Hallegraeff , G. M. Vol. 41 , 405 – 426 . Berlin : Springer-Verlag . NATO ASI Series
   Google Scholar
• BATES , S. S. , GARRISON , D. L. and HORNER , R. A. 1998 . “ Bloom dynamics and physiology of domoic-acid-producing Pseudo-nitzschia species ” . In Physiological Ecology of Harmful Algal Blooms , Edited by: Anderson , D. M. , Cembella , A. D. and Hallegraeff , G. M. Vol. 41 , 267 – 292 . Berlin : Springer-Verlag . NATO ASI Series
   Google Scholar
• BATES , S. S , BIRD , C. J. , DE FREITAS , A. S. W. , FOXALL , M. , HANIC , L. A. , JOHNSON , G. R. , MCCULLOCH , A. W. , ODENSE , P. , POCKLINGTON , R. , QUILLIAM , M. A. , SIM , P. G. , SMITH , J. C , SUBBA RAO , D. V. , TODD , E. C. D. , WALTER , J. A. and WRIGHT , J. L. C. 1989 . Pennate diatom Nitzschia pungens as the primary source of domoic acid, a toxin in shellfish from eastern Prince Edward Island, Canada . Canadian Journal of Fisheries and Aquatic Sciences , 46 : 1203 – 1215 .
   Web of Science ®Google Scholar
• BUCK , K. R. , UTTAL-COOKE , L. , PILSKALN , C. H. , ROELKE , D. L. , VILLAC , M. C , FRYXELL , G. A. , CIFUENTES , L. and CHAVEZ , F. P. 1992 . Autecology of the diatom Pseudonitzschia australis, a domoic acid producer, from Monterey Bay, California . Marine Ecology Progress Series , 84 : 293 – 302 .
   Web of Science ®Google Scholar
• CAROPPO , C. , CONGESTRI , R. , BRACCHINI , L. and ALBERTANO , P. 2005 . On the presence of Pseudo-nitzschia calliantha Lundholm, Moestrup et Hasle and Pseudo-nitzschia delicatissima (Cleve) Heiden in the Southern Adriatic Sea (Mediterranean Sea, Italy) . Journal of Plankton Research , 27 : 763 – 774 .
   Web of Science ®Google Scholar
• CANDEIAS , A. 1939 . Microplancton da região da foz do Douro . Boletim da Sociedade Broteriana, (sér. 2) , 13 : 237 – 272 .
   Google Scholar
• CARRISSO , L. 1911 . Materiaes para o estudo do plancton na costa portuguêsa (1° fascículo) . Boletim da Sociedade Broteriana , 26 : 5 – 82 .
   Google Scholar
• CASTELEYN , G. , CHEPURNOV , V. A. , LELIAERT , F. , MANN , D. G. , BATES , S. S. , LUNDHOLM , N. , RHODES , L. , SABBE , K. and VYVERMAN , W. 2008 . Pseudo-nitzschia pungens (Bacillariophyceae): a cosmopolitan species? . Harmful Algae , 7 : 241 – 257 .
   Web of Science ®Google Scholar
• CHEPURNOV , V. A. , MANN , D. G. , SABBE , K. , VANNERUM , K. , CASTELEUN , C. , VERLEYEN , E. , PEPERZAK , L. and VYVERMAN , W. 2005 . Sexual reproduction, mating system, chloroplast dynamics and abrupt cell size reduction in Pseudo-nitzschia pungens from the North Sea (Bacillariophyta) . European Journal of Phycology , 40 : 379 – 395 .
   Web of Science ®Google Scholar
• CLEVE , P. T. 1897 . “ A treatise on the phytoplankton of the Atlantic and its tributaries and on the periodical changes of the plankton of Skagerrak ” . In Upsala Nya Tidnings Aktiebolags Tryckeri 27 Uppsala
   Google Scholar
• CLEVE , P. T. and MÖLLER , J. D. 1882 . Diatoms Vol. 6 , 1 – 6 . Uppsala (Nr 277–324)
   Google Scholar
• COLEMAN , A. W. 2000 . The significance of a coincidence between evolutionary landmarks found in mating affinity and a DNA sequence . Protist , 151 : 1 – 9 .
   PubMed Web of Science ®Google Scholar
• COSTA , P. R. and GARRIDO , S. 2004 . Domoic acid accumulation in the sardine Sardina pilchardus and its relationship to Pseudo-nitzschia diatom ingestion . Marine Ecology Progress Series , 284 : 261 – 268 .
   Web of Science ®Google Scholar
• CUSACK , C. K. , RAINE , R. and PATCHING , J. W. 2004 . Occurrence of species from the genus Pseudo-nitzschia Peragallo in Irish waters . Biology and Environment: Proceedings of the Royal Irish Academy , 104B : 55 – 74 .
   Google Scholar
• CUSACK , C. K. , BATES , S. S. , QUILLIAM , M. A. , PATCHING , J. W. and RAINE , R. 2002 . Confirmation of domoic production by Pseudo-nitzschia australis (Bacillariophyceae) isolated from Irish waters . Journal of Phycology , 38 : 1106 – 1112 .
   Web of Science ®Google Scholar
• DIAS , J. M. , LOPES , J. F. and DEKEYSER , I. 1999 . Hydrological characterisation of Ria de Aveiro, Portugal, in early summer . Oceanologica Acta , 22 : 473 – 185 .
   Google Scholar
• ESTRADA , M. 1995 . “ Dinoflagellate assemblages in the Iberian upwelling area ” . In Harmful Marine Algal Blooms , Edited by: Lassus , P. , Arzul , G. , Erard , E. , Gentien , P. and Marcaillou , C. 157 – 162 . Lavoisier : Intercept Ltd. .
   Google Scholar
• FEHLING , J. , DAVIDSON , K. and BATES , S. S. 2005 . Growth dynamics of non-toxic Pseudo-nitzschia delicatissima and toxic P. seriata (Bacillariophyceae) under simulated spring and summer photoperiods . Harmful Algae , 4 : 763 – 769 .
   Web of Science ®Google Scholar
• FEHLING , J. , GREEN , D. H. , DAVIDSON , K. , BOLCH , C. J. and BATES , S. S. 2004 . Domoic acid production by Pseudo-nitzschia seriata (Bacillariophyceae) in Scottish waters . Journal of Phycology. , 40 : 622 – 630 .
   Web of Science ®Google Scholar
• FRAGA , S. , ALVAREZ , M. J. , MÍGUEZ , Á. , FERNÁNDEZ , M. L. , COSTAS , E. and LÓPEZ , V. 1998 . “ Pseudo-nitzschia species isolated from Galician waters: toxicity, DNA content and lectin binding assay ” . In Harmful Algae , Edited by: Reguera , B. , Blanco , J. , Fernández , M. L. and Wyatt , T. 270 – 273 . Spain : International Oceanographic Commission of UNESCO .
   Google Scholar
• FRYXELL , G. A. and HASLE , G. R. 2003 . “ Taxonomy of harmful diatoms ” . In Manual on Harmful Marine Microalgae , Edited by: Hallegraeff , G. M. , Anderson , D. M. and Cembella , A. D. 465 – 509 . France : UNESCO Publishing .
   Google Scholar
• GUILLARD , R. R. L. 1975 . “ Culture of phytoplankton for feeding marine invertebrates ” . In Culture of marine invertebrate animals. , Edited by: Smith , W. L. and Chanley , M. H. 29 – 60 . New York : Plenum Press .
   Google Scholar
• HASLE , G. R. 1965 . “ Nitzschia and Fragilariopsis species studied in the light and electron microscopes II ” . In The group Pseudo-nitzschia. Skrifter utgitt av Det Norske Videnskaps-Akademi i Oslo I Matematisk-Naturvidenskapelige Klasse. , 45 Oslo : Universitetsforlaget . Ny Serie. No. 18
   Google Scholar
• HASLE , G. R. 1972 . The distribution of Nitzschia seriata Cleve and allied species . Nova Hedwigia, Beiheft , 39 : 171 – 190 .
   Google Scholar
• HASLE , G. R. 1995 . Pseudo-nitzschia pungens and P. multiseries (Bacillariophyceae): Nomenclature history, morphology, and distribution . Journal of Phycology , 31 : 428 – 135 .
   Web of Science ®Google Scholar
• HASLE , G. R. 2002 . Are most of the domoic acid-producing species of the diatom genus Pseudo-nitzschia cosmopolites? . Harmful Algae , 1 : 137 – 146 .
   Google Scholar
• HASLE , G. R. , LANGE , C. B. and SYVERTSEN , E. E. 1996 . A review of Pseudo-nitzschia, with special reference to the Skagerrak, North Atlantic, and adjacent waters . Helgoländer Meeresuntersuchungen , 50 : 131 – 175 .
   Google Scholar
• JACKSON , A. E. , AYER , S. W. and LAYCOCK , M. V. 1992 . The effect of salinity on growth and amino acid composition in the marine diatom Nitzschia pungens . Canadian Journal of Botany , 70 : 2198 – 2201 .
   Google Scholar
• KACZMARSKA , I. , LEGRESLEY , M. M. , MARTIN , J. L. and EHRMAN , J. 2005 . Diversity of the diatom genus Pseudo-nitzschia Peragallo in the Quoddy Region of the Bay of Fundy, Canada . Harmful Algae , 4 : 1 – 19 .
   Web of Science ®Google Scholar
• KOTAKI , Y. , KOIKE , K. , YOSHIDA , M. , THUOC , C. V. , HUYEN , N. T. M. , HOI , N. C , FUKUYO , Y. and KODAMA , M. 2000 . Domoic acid production in Nitzschia sp. (Bacillariophyceae) isolated from a shrimp-culture pond in Do Son, Vietnam . Journal of Phycology , 36 : 1057 – 1060 .
   Web of Science ®Google Scholar
• LEWIS , N. I. , BATES , S. S. , MCLACHLAN , J. L. and SMITH , J. C. 1993 . “ Temperature effects on growth, domoic acid production, and morphology of the diatom Nitzschia pungens f. multiseries. ” . In Toxic phytoplankton blooms in the sea , Edited by: Smayda , T. J. and Shimizu , Y. 601 – 613 . Amsterdam : Elsevier Science Publishers .
   Google Scholar
• LUNDHOLM , N. and MOESTRUP , Ø. 2000 . Morphology of the marine diatom Nitzschia navisvaringica, sp. nov. (Bacillariophyceae), another producer of the neurotoxin domoic acid . Journal of Phycology , 36 : 1162 – 1174 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. and MOESTRUP , Ø. 2002 . The marine diatom Pseudo-nitzschia galaxiae sp. nov. (Bacillariophyceae): morphology and phylogenetic relationships . Phycologia , 41 : 594 – 605 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. and MOESTRUP , Ø . 2007 . “ Diatoms ” . In IOC Taxonomic Reference List of Toxic Plankton Algae , Intergovernmental Oceanographic Commission of UNESCO . www.bi.ku.dk/ioc/default.asp
   Google Scholar
• LUNDHOLM , N. , HASLE , G. R. , FRYXELL , G. A. and HARGRAVES , P. E. 2002 . Morphology and taxonomy of species within the Pseudo-nitzschia americana complex (Bacillariophyceae) with descriptions of two new species, Pseudo-nitzschia brasiliana and Pseudo-nitzschia linea . Phycologia , 41 : 480 – 497 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. , MOESTRUP , Ø. , HASLE , G. R. and HOEF-EMDEN , K. 2003 . A study of the Pseudo-nitzschia pseudodelicatissima/cuspidata complex (Bacillariophyceae): what is P. pseudodelicatissima? . Journal of Phycology , 39 : 797 – 813 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. , SKOV , J. , POCKLINGTON , R. and MOESTRUP , Ø. 1997 . Studies on the marine planktonic diatom Pseudo-nitzschia. 2. Autecology of P. pseudodelicatissima based on isolates from Danish coastal waters . Phycologia , 36 : 381 – 388 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. , MOESTRUP , Ø. , KOTAKI , Y. , HOEF-EMDEN , K. , SCHOLIN , C. and MILLER , P. 2006 . Inter- and intraspecific variation of the Pseudo-nitzschia delicatissima complex (Bacillariophyceae) illustrated by rRNA probes, morphological data and phylogenetic analyses . Journal of Phycology , 42 : 464 – 481 .
   Web of Science ®Google Scholar
• LUNDHOLM , N. , SKOV , J. , POCKLINGTON , R. and MOESTRUP , Ø. 1994 . Domoic acid, the toxic amino acid responsible for amnesic shellfish poisoning, now in Pseudo-nitzschia seriata (Bacillariophyceae) in Europe . Phycologia , 33 : 475 – 478 .
   Web of Science ®Google Scholar
• MAI , J. C. and COLEMAN , A. W. 1997 . The internal transcribed spacer 2 exhibits a common secondary structure in green algae and flowering plants . Journal of Molecular Evolution , 44 : 258 – 271 .
   PubMed Web of Science ®Google Scholar
• MILLER , P. E. and SCHOLIN , S. A. 1998 . Identification and enumeration of cultured and wild Pseudo-nitzschia (Bacillariophyceae) using species-specific LSU rRNA-targeted fluorescent probes and filter-based whole cell hybridisation . Jounal of Phycology , 34 : 371 – 382 .
   Web of Science ®Google Scholar
• MOITA , M. T. 1993 . “ Development of toxic dinoflagellates in relation to upwelling patterns off Portugal ” . In Toxic Phytoplankton Blooms in the Sea , Edited by: Smayda , T. J. and Shimizu , Y. 299 – 304 . Amsterdam : Elsevier Science Publishers .
   Google Scholar
• MOITA , M. T. and VILARINHO , M. G. 1999 . Checklist of phytoplankton species off Portugal: 70 years of studies . Portugaliae Acta Biologica (série B, Sistemática) , 18 : 5 – 50 .
   Google Scholar
• ORLOVA , T. Y. and SHEVCHENKO , O. G. 2002 . The first finding of Pseudo-nitzschia americana (Bacillariophyta) in Russian Seas . Russian Journal of Marine Biology , 28 : 336 – 339 .
   Google Scholar
• ORSINI , L. , SARNO , D. , PROCACCINI , G. , POLETTI , R. , DAHLMANN , J. and MONTRESOR , M. 2002 . Toxic Pseudo-nitzschia multistriata (Bacillariophyceae) from the Gulf of Naples: morphology, toxin analysis and phylogenetic relationships with other Pseudo-nitzschia species . European Journal of Phycology , 37 : 247 – 257 .
   Web of Science ®Google Scholar
• PAN , Y. , BATES , S. S. and CEMBELLA , A. D. 1998 . Environmental stress and domoic acid production by Pseudo-nitzschia: a physiological perspective . Natural Toxins , 6 : 127 – 135 .
   PubMedGoogle Scholar
• PARSONS , M. L. , SCHOLIN , C. A. , MILLER , P. E. , DOUCETTE , G. J. , POWELL , C. L. , FRYXELL , G. A. , DORTCH , Q. and SONIAT , T. M. 1999 . Pseudo-nitzschia (Bacillariophyceae) in Louisiana coastal waters: molecular probes field trials, genetic variability, and domoic acid analyses . Journal of Phycology , 35 : 1368 – 1378 .
   Web of Science ®Google Scholar
• PINTO , J. S. and SILVA , E. S. 1956 . The toxicity of Cardium edule L. and its possible relation to the dinoflagellate Prorocentrum micans Ehr . Notas e Estudos do Instituto de Biologia Maritima , 12 : 1 – 20 . pl. I
   Google Scholar
• POSADA , D. and CRANDALL , K. A. 1998 . Modeltest: testing the model of DNA substitution . Bioinformatics Applications Note , 14 : 817 – 818 .
   PubMed Web of Science ®Google Scholar
• RHODES , L. 1998 . Identification of potentially toxic Pseudo-nitzschia (Bacillariophyceae) in New Zealand coastal waters, using lectins . New Zealand Journal of Marine and Freshwater Research , 32 : 537 – 544 .
   Web of Science ®Google Scholar
• RHODES , L. , ADAMSON , J. and SCHOLIN , C. 2000 . Pseudo-nitzschia multistriata (Bacillariophyceae) in New Zealand . New Zealand Journal of Marine and Freshwater Research , 34 : 463 – 467 .
   Web of Science ®Google Scholar
• SILVA , E. S. 1952 . Estudos de plancton na Lagoa de Óbidos I. Diatomáceas e dinoflagelados . Revista da Faculdade de Ciências de Lisboa, (2^a sér.-C) , 2 : 5 – 44 .
   Google Scholar
• SILVA , E. S. and PINTO , J. S. 1948 . O plancton da baía de S. Martinho do Porto—I. Diatomáceas e dinoflagelados . Boletim da Sociedade Portuguesa de Ciências Naturais , 16 : 134 – 187 .
   Google Scholar
• SILVA , M. M. C. 1990 . Some notes about the phytoplanktonic community in river Lima . Publicações do Instituto de Zoologia “Dr. Augusto Nobre” , 222 : 1 – 21 .
   Google Scholar
• SKOV , J. , LUNDHOLM , N. , MOESTRUP , Ø. and LARSEN , J. 1999 . “ Potentially toxic phytoplankton 4. The diatom genus Pseudo-nitzschia (Diatomophyceae/Bacillariophyceae) ” . In ICES Identification leaflets for plankton , Edited by: Lindley , J. A. 1 – 23 . Denmark : International Council for the exploration of the sea . Leaflet no. 185
   Google Scholar
• STEHR , C. M. , CONNELL , L. , BAUGH , K. A. , BILL , B. D. , ADAMS , N. G. and TRAINER , V. L. 2002 . Morphological, toxicological, and genetic differences among Pseudo-nitzschia (Bacillariophyceae) species in inland embayments and outer coastal waters of Washington state, USA . Journal of Phycology , 38 : 55 – 65 .
   Web of Science ®Google Scholar
• SWOFFORD , D. L. 2001 . PAUP*. Phylogenetic Analysis Using Parsimony (*and other methods). , Version 4 , Sunderland, MA : Sinauer Associates .
   Google Scholar
• TRAINER , V. L. , HICKEY , B. M. and BATES , S. S. 2008 . “ Toxic diatoms ” . In Oceans and human health , 219 – 237 . San Diego, , US : Academic Press .
   Google Scholar
• TRAINER , V. L. , WEKELL , J. C , HORNER , R. A. , HATFIELD , C. L. and STEIN , J. E. 1998 . “ Domoic acid production by Pseudo-nitzschia pungens. ” . In Harmful Algae , Edited by: Reguera , B. , Blanco , J. , Fernández , M. L. and Wyatt , T. 337 – 340 . Spain : International Oceanographic Commission of UNESCO .
   Google Scholar
• TRAINER , V. L. , ADAMS , N. G. , BILL , B. D. , STEHR , C. M. , WEKELL , J. C. , MOELLER , P. , BUSMAN , M. and WOODRUFF , D. 2000 . Domoic acid production near California coastal upwelling zones, June 1998 . Limnology and Oceanography , 45 : 1818 – 1833 .
   Web of Science ®Google Scholar
• THESSEN , A. E. , DORTCH , Q. , PARSONS , M. L. and MORRISSON , W. 2005 . Effect of salinity on Pseudo-nitzschia species (Bacillariophyceae) growth and distribution . Journal of Phycology , 41 : 21 – 29 .
   Web of Science ®Google Scholar
• VALE , P. and SAMPAYO , M. A. M. 2001 . Domoic acid in Portuguese shellfish and fish . Toxicon , 39 : 893 – 904 .
   PubMed Web of Science ®Google Scholar
• VALE , P. , SAMPAYO , M. A. M. and QUILLIAM , M. A. 1998 . “ DSP complex toxin profiles relation with Dinophysis spp. occurrence and domoic acid confirmation by LC-MS in Portuguese bivalves ” . In Harmful Algae , Edited by: Reguera , B. , Blanco , J. , Fernández , M. L. and Wyatt , T. 503 – 506 . Spain : International Oceanographic Commission of UNESCO .
   Google Scholar
• VALE , P. , BOTELHO , M. J. , RODRIGUES , S. M. , GOMES , S. S. and SAMPAYO , M. A. M. 2008 . Two decades of marine biotoxin monitoring in bivalves from Portugal (1986–2006): a review of exposure assessment . Harmful Algae , 7 : 11 – 25 .
   Web of Science ®Google Scholar
• VILLAC , M. C. and FRYXELL , G. A. 1998 . Pseudo-nitzschia pungens var. cingulata var. nov. (Bacillariophyceae) based on field and culture observations . Phycologia , 37 : 269 – 274 .
   Web of Science ®Google Scholar
• ZUKER , M. 2003 . Mfold web server for nucleic acid folding and hybridization prediction . Nucleic Acids Research , 31 : 3406 – 3415 .
   PubMed Web of Science ®Google Scholar