Optisol-GS Storage of Cultured Human Limbal Epithelial Cells at Ambient Temperature Is Superior to Hypothermic Storage

References

• Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. The Lancet. 1997;349(9057):990–93. doi:10.1016/S0140-6736(96)11188-0.
   PubMed Web of Science ®Google Scholar
• Tsai RJ-F, Li L-M, Chen J-K. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med. 2000;343:86–93. doi:10.1056/NEJM200007133430202.
   PubMed Web of Science ®Google Scholar
• Pellegrini G, Lambiase A, Macaluso C, Pocobelli A, Deng S, Cavallini GM, Esteki R, Rama P. From discovery to approval of an advanced therapy medicinal product-containing stem cells, in the EU. Regen Med. 2016;11:407–20. doi:10.2217/rme-2015-0051.
   PubMed Web of Science ®Google Scholar
• Ahmad S, Osei-Bempong C, Dana R, Jurkunas U. The culture and transplantation of human limbal stem cells. J Cell Physiol. 2010;225:15–19. doi:10.1002/jcp.22251.
   PubMed Web of Science ®Google Scholar
• Oie Y, Nozaki T, Takayanagi H, Hara S, Hayashi R, Takeda S, Mori K, Moriya N, Soma T, Tsujikawa M, et al. Development of a cell sheet transportation technique for regenerative medicine. Tissue Eng Part C Methods. 2014;20:373–82. doi:10.1089/ten.tec.2013.0266.
   PubMed Web of Science ®Google Scholar
• Vasania VS, Hari A, Tandon R, Shah S, Haldipurkar S, Shah S, Sachan S, Viswanathan C. Transplantation of autologous ex vivo expanded human conjunctival epithelial cells for treatment of pterygia: a prospective open-label single arm multicentric clinical trial. J Ophthalmic Vis Res. 2014;9:407–16. doi:10.4103/2008-322X.150800.
   PubMedGoogle Scholar
• Summerlin WT, Miller GE, Harris JE, Good RA. The organ-cultured cornea: an in vitro study. Invest Ophthalmol. 1973;12:176–80.
   PubMedGoogle Scholar
• Utheim TP, Raeder S, Utheim OA, Cai Y, Roald B, Drolsum L, Lyberg T, Nicolaissen B. A novel method for preserving cultured limbal epithelial cells. Br J Ophthalmol. 2007;91:797–800. doi:10.1136/bjo.2006.103218.
   PubMed Web of Science ®Google Scholar
• Medicinal and other products and human and animal transmissible spongiform encephalopathies: memorandum from a WHO meeting. Bull World Health Organ. 1997;75:505–13.
   PubMed Web of Science ®Google Scholar
• Mannello F, Tonti GA. Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells. 1603-1609;2007(25).
   Google Scholar
• Utheim O, Islam R, Lyberg T, Roald B, Eidet JR, de la Paz MF, Dartt DA, Raeder S, Utheim TP. Serum-free and xenobiotic-free preservation of cultured human limbal epithelial cells. PLoS One. 2015;10(3):e0118517. doi:10.1371/journal.pone.0118517.
   PubMed Web of Science ®Google Scholar
• Lass JH, Gordon JF, Sugar A, Norden RA, Reinhart WJ, Meyer RF, Soong HK. Optisol containing streptomycin. Am J Ophthalmol. 1993;116:503–04. doi:10.1016/S0002-9394(14)71413-8.
   PubMed Web of Science ®Google Scholar
• Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol. 1997;123:303–12. doi:10.1016/S0002-9394(14)70125-4.
   PubMed Web of Science ®Google Scholar
• Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. British J Ophthalmol. 2002;86:463–71. doi:10.1136/bjo.86.4.463.
   PubMed Web of Science ®Google Scholar
• Utheim OA, Pasovic L, Raeder S, Eidet JR, Fostad IG, Sehic A, Roald B, de la Paz MF, Lyberg T, Dartt DA, et al. Effects of explant size on epithelial outgrowth, thickness, stratification, ultrastructure and phenotype of cultured limbal epithelial cells. PLoS One. 2019;14:e0212524. doi:10.1371/journal.pone.0212524.
   PubMed Web of Science ®Google Scholar
• Kelman Z. PCNA: structure, functions and interactions. Oncogene. 1997;14:629–40. doi:10.1038/sj.onc.1200886.
   PubMed Web of Science ®Google Scholar
• Selver OB, Barash A, Ahmed M, Wolosin JM. ABCG2-dependent dye exclusion activity and clonal potential ineEpithelial cells continuously growing for one month from limbal explants. Invest Ophthalmol Vis Sci. 2011;52:4330–37. doi:10.1167/iovs.10-5897.
   PubMed Web of Science ®Google Scholar
• Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996;183:1797–806. doi:10.1084/jem.183.4.1797.
   PubMed Web of Science ®Google Scholar
• Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med. 2010;363:147–55. doi:10.1056/NEJMoa0905955.
   PubMed Web of Science ®Google Scholar
• Utheim OA, Lyberg T, Eidet JR, Raeder S, Sehic A, Roald B, Messelt E, de la Paz MF, Dartt DA, Utheim TP. Effect of transportation on cultured limbal epithelial sheets for worldwide treatment of limbal stem cell deficiency. Sci Rep. 2018;8:10502. doi:10.1038/s41598-018-28553-0.
   PubMed Web of Science ®Google Scholar
• Raeder S, Utheim TP, Olstad OK, de la Paz MF, Lyberg T. Genome-wide transcriptional analysis of cultured human limbal epithelial cells following hypothermic storage Optisol-GS. Invest Ophthalmol Vis Sci. 2009;50:E-Abstract1773/A1441. Available at www.iovs.org.
   PubMed Web of Science ®Google Scholar
• Raeder S, Utheim TP, Utheim OA, Nicolaissen B, Roald B, Cai Y, Haug K, Kvalheim A, Messelt EB, Drolsum L, et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium. Invest Ophthalmol Vis Sci. 2007;48:5484–93. doi:10.1167/iovs.07-0494.
   PubMed Web of Science ®Google Scholar
• Schwab IR, Johnson NT, Harkin DG. Inherent risks associated with manufacture of bioengineered ocular surface tissue. Arch Ophthalmol. 2006;124:1734–40. doi:10.1001/archopht.124.12.1734.
   PubMed Web of Science ®Google Scholar
• McCarey BE, Kaufman HE. Improved corneal storage. Invest Ophthalmol. 1974;13:165–73.
   PubMedGoogle Scholar
• Jeng BH. Preserving the cornea: corneal storage media. Curr Opin Ophthalmol. 2006;17:322–27. doi:10.1097/01.icu.0000233950.63853.88.
   Web of Science ®Google Scholar
• Armitage WJ, Easty DL. Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci. 1997;38:16–24.
   PubMed Web of Science ®Google Scholar
• Tungsiripat T, Sarayba MA, Taban M, Sweet PM, Osann KE, Chuck RS. Viability of limbal epithelium after anterior lamellar harvesting using a microkeratome. Ophthalmology. 2004;111:469–75. doi:10.1016/j.ophtha.2003.06.012.
   PubMed Web of Science ®Google Scholar
• Means TL, Geroski DH, L’Hernault N, Grossniklaus HE, Kim T, Edelhauser HF. The corneal epithelium after optisol-GS storage. Cornea. 1996;15:599–605. doi:10.1097/00003226-199611000-00010.
   PubMed Web of Science ®Google Scholar
• Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001;7:1028–34. doi:10.1038/nm0901-1028.
   PubMed Web of Science ®Google Scholar