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Cornea

Optisol-GS Storage of Cultured Human Limbal Epithelial Cells at Ambient Temperature Is Superior to Hypothermic Storage

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Pages 1497-1503 | Received 06 Feb 2020, Accepted 04 May 2020, Published online: 24 Jun 2020

References

  • Pellegrini G, Traverso CE, Franzi AT, Zingirian M, Cancedda R, De Luca M. Long-term restoration of damaged corneal surfaces with autologous cultivated corneal epithelium. The Lancet. 1997;349(9057):990–93. doi:10.1016/S0140-6736(96)11188-0.
  • Tsai RJ-F, Li L-M, Chen J-K. Reconstruction of damaged corneas by transplantation of autologous limbal epithelial cells. N Engl J Med. 2000;343:86–93. doi:10.1056/NEJM200007133430202.
  • Pellegrini G, Lambiase A, Macaluso C, Pocobelli A, Deng S, Cavallini GM, Esteki R, Rama P. From discovery to approval of an advanced therapy medicinal product-containing stem cells, in the EU. Regen Med. 2016;11:407–20. doi:10.2217/rme-2015-0051.
  • Ahmad S, Osei-Bempong C, Dana R, Jurkunas U. The culture and transplantation of human limbal stem cells. J Cell Physiol. 2010;225:15–19. doi:10.1002/jcp.22251.
  • Oie Y, Nozaki T, Takayanagi H, Hara S, Hayashi R, Takeda S, Mori K, Moriya N, Soma T, Tsujikawa M, et al. Development of a cell sheet transportation technique for regenerative medicine. Tissue Eng Part C Methods. 2014;20:373–82. doi:10.1089/ten.tec.2013.0266.
  • Vasania VS, Hari A, Tandon R, Shah S, Haldipurkar S, Shah S, Sachan S, Viswanathan C. Transplantation of autologous ex vivo expanded human conjunctival epithelial cells for treatment of pterygia: a prospective open-label single arm multicentric clinical trial. J Ophthalmic Vis Res. 2014;9:407–16. doi:10.4103/2008-322X.150800.
  • Summerlin WT, Miller GE, Harris JE, Good RA. The organ-cultured cornea: an in vitro study. Invest Ophthalmol. 1973;12:176–80.
  • Utheim TP, Raeder S, Utheim OA, Cai Y, Roald B, Drolsum L, Lyberg T, Nicolaissen B. A novel method for preserving cultured limbal epithelial cells. Br J Ophthalmol. 2007;91:797–800. doi:10.1136/bjo.2006.103218.
  • Medicinal and other products and human and animal transmissible spongiform encephalopathies: memorandum from a WHO meeting. Bull World Health Organ. 1997;75:505–13.
  • Mannello F, Tonti GA. Concise review: no breakthroughs for human mesenchymal and embryonic stem cell culture: conditioned medium, feeder layer, or feeder-free; medium with fetal calf serum, human serum, or enriched plasma; serum-free, serum replacement nonconditioned medium, or ad hoc formula? All that glitters is not gold! Stem Cells. 1603-1609;2007(25).
  • Utheim O, Islam R, Lyberg T, Roald B, Eidet JR, de la Paz MF, Dartt DA, Raeder S, Utheim TP. Serum-free and xenobiotic-free preservation of cultured human limbal epithelial cells. PLoS One. 2015;10(3):e0118517. doi:10.1371/journal.pone.0118517.
  • Lass JH, Gordon JF, Sugar A, Norden RA, Reinhart WJ, Meyer RF, Soong HK. Optisol containing streptomycin. Am J Ophthalmol. 1993;116:503–04. doi:10.1016/S0002-9394(14)71413-8.
  • Lee SH, Tseng SC. Amniotic membrane transplantation for persistent epithelial defects with ulceration. Am J Ophthalmol. 1997;123:303–12. doi:10.1016/S0002-9394(14)70125-4.
  • Meller D, Pires RT, Tseng SC. Ex vivo preservation and expansion of human limbal epithelial stem cells on amniotic membrane cultures. British J Ophthalmol. 2002;86:463–71. doi:10.1136/bjo.86.4.463.
  • Utheim OA, Pasovic L, Raeder S, Eidet JR, Fostad IG, Sehic A, Roald B, de la Paz MF, Lyberg T, Dartt DA, et al. Effects of explant size on epithelial outgrowth, thickness, stratification, ultrastructure and phenotype of cultured limbal epithelial cells. PLoS One. 2019;14:e0212524. doi:10.1371/journal.pone.0212524.
  • Kelman Z. PCNA: structure, functions and interactions. Oncogene. 1997;14:629–40. doi:10.1038/sj.onc.1200886.
  • Selver OB, Barash A, Ahmed M, Wolosin JM. ABCG2-dependent dye exclusion activity and clonal potential ineEpithelial cells continuously growing for one month from limbal explants. Invest Ophthalmol Vis Sci. 2011;52:4330–37. doi:10.1167/iovs.10-5897.
  • Goodell MA, Brose K, Paradis G, Conner AS, Mulligan RC. Isolation and functional properties of murine hematopoietic stem cells that are replicating in vivo. J Exp Med. 1996;183:1797–806. doi:10.1084/jem.183.4.1797.
  • Rama P, Matuska S, Paganoni G, Spinelli A, De Luca M, Pellegrini G. Limbal stem-cell therapy and long-term corneal regeneration. N Engl J Med. 2010;363:147–55. doi:10.1056/NEJMoa0905955.
  • Utheim OA, Lyberg T, Eidet JR, Raeder S, Sehic A, Roald B, Messelt E, de la Paz MF, Dartt DA, Utheim TP. Effect of transportation on cultured limbal epithelial sheets for worldwide treatment of limbal stem cell deficiency. Sci Rep. 2018;8:10502. doi:10.1038/s41598-018-28553-0.
  • Raeder S, Utheim TP, Olstad OK, de la Paz MF, Lyberg T. Genome-wide transcriptional analysis of cultured human limbal epithelial cells following hypothermic storage Optisol-GS. Invest Ophthalmol Vis Sci. 2009;50:E-Abstract1773/A1441. Available at www.iovs.org.
  • Raeder S, Utheim TP, Utheim OA, Nicolaissen B, Roald B, Cai Y, Haug K, Kvalheim A, Messelt EB, Drolsum L, et al. Effects of organ culture and Optisol-GS storage on structural integrity, phenotypes, and apoptosis in cultured corneal epithelium. Invest Ophthalmol Vis Sci. 2007;48:5484–93. doi:10.1167/iovs.07-0494.
  • Schwab IR, Johnson NT, Harkin DG. Inherent risks associated with manufacture of bioengineered ocular surface tissue. Arch Ophthalmol. 2006;124:1734–40. doi:10.1001/archopht.124.12.1734.
  • McCarey BE, Kaufman HE. Improved corneal storage. Invest Ophthalmol. 1974;13:165–73.
  • Jeng BH. Preserving the cornea: corneal storage media. Curr Opin Ophthalmol. 2006;17:322–27. doi:10.1097/01.icu.0000233950.63853.88.
  • Armitage WJ, Easty DL. Factors influencing the suitability of organ-cultured corneas for transplantation. Invest Ophthalmol Vis Sci. 1997;38:16–24.
  • Tungsiripat T, Sarayba MA, Taban M, Sweet PM, Osann KE, Chuck RS. Viability of limbal epithelium after anterior lamellar harvesting using a microkeratome. Ophthalmology. 2004;111:469–75. doi:10.1016/j.ophtha.2003.06.012.
  • Means TL, Geroski DH, L’Hernault N, Grossniklaus HE, Kim T, Edelhauser HF. The corneal epithelium after optisol-GS storage. Cornea. 1996;15:599–605. doi:10.1097/00003226-199611000-00010.
  • Zhou S, Schuetz JD, Bunting KD, Colapietro AM, Sampath J, Morris JJ, Lagutina I, Grosveld GC, Osawa M, Nakauchi H, et al. The ABC transporter Bcrp1/ABCG2 is expressed in a wide variety of stem cells and is a molecular determinant of the side-population phenotype. Nat Med. 2001;7:1028–34. doi:10.1038/nm0901-1028.

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