MiR-34a-5p Negatively Regulates Oxidative Stress on Lens Epithelial Cells by Silencing GPX3 – A Novel Target

References

• Truscott RJ. Age-related nuclear cataract-oxidation is the key. Exp Eye Res. 2005;80(5):709–725. doi:10.1016/j.exer.2004.12.007.
   PubMed Web of Science ®Google Scholar
• Babizhayev MA. Generation of reactive oxygen species in the anterior eye segment. Synergistic codrugs of N-acetylcarnosine lubricant eye drops and mitochondria-targeted antioxidant act as a powerful therapeutic platform for the treatment of cataracts and primary open-angle glaucoma. BBA Clin. 2016;6:49–68. doi:10.1016/j.bbacli.2016.04.004.
   PubMed Web of Science ®Google Scholar
• Sacca SC, Cutolo CA, Ferrari D, Corazza P, Traverso CE. The eye, oxidative damage and polyunsaturated fatty acids. Nutrients. 2018;10(6):668. doi:10.3390/nu10060668.
   PubMed Web of Science ®Google Scholar
• Lou MF. Redox regulation in the lens. Prog Retin Eye Res. 2003;22(5):657–682. doi:10.1016/S1350-9462(03)00050-8.
   PubMed Web of Science ®Google Scholar
• Tsang CK, Liu Y, Thomas J, Zhang YJ, Zheng XF. Superoxide dismutase 1 acts as a nuclear transcription factor to regulate oxidative stress resistance. Nat Commun. 2014;5:3446. doi:10.1038/ncomms4446.
   PubMed Web of Science ®Google Scholar
• Ivanov IV, Mappes T, Schaupp P, Lappe C, Wahl S. Ultraviolet radiation oxidative stress affects eye health. J Biophotonics. 2018;11(7):e201700377. doi:10.1002/jbio.201700377.
   PubMed Web of Science ®Google Scholar
• Giblin FJ. Glutathione: a vital lens antioxidant. J Ocul Pharmacol Ther. 2000;16(2):121–135. doi:10.1089/jop.2000.16.121.
   PubMed Web of Science ®Google Scholar
• Beebe DC, Holekamp NM, Shui YB. Oxidative damage and the prevention of age-related cataracts. Ophthalmic Res. 2010;44(3):155–165. doi:10.1159/000316481.
   PubMed Web of Science ®Google Scholar
• Toda J, Kato S, Oshika T, Sugita G. Posterior capsule opacification after combined cataract surgery and vitrectomy. J Cataract Refract Surg. 2007;33(1):104–107. doi:10.1016/j.jcrs.2006.09.018.
   PubMed Web of Science ®Google Scholar
• He L, Hannon GJ. MicroRNAs: small RNAs with a big role in gene regulation. Nat Rev Genet. 2004;5(7):522–531. doi:10.1038/nrg1379.
   PubMed Web of Science ®Google Scholar
• Wu CR, Lin HT, Wang QL, Chen W, Luo HH, Chen WR, Zhang H. Discrepant expression of microRNAs in transparent and cataractous human lenses. Invest Ophthalmol Vis Sci. 2012;53(7):3906–3912. doi:10.1167/iovs.11-9178.
   PubMed Web of Science ®Google Scholar
• Chen XY, Xiao W, Chen WR, Liu XL, Wu MX, Bo Q, Luo Y, Ye SB, Cao YH, Liu YZ. MicroRNA-26a and -26b inhibit lens fibrosis and cataract by negatively regulating Jagged-1/Notch signaling pathway. Cell Death Differ. 2017;24(8):1431–1442. doi:10.1038/cdd.2016.152.
   PubMed Web of Science ®Google Scholar
• Wang S, Guo CJ, Yu MS, Ning XN, Yan B, Zhao J, Yang AG, Yan H. 2018. Identification of H2O2 induced oxidative stress associated microRNAs in HLE-B3 cells and their clinical relevance to the progression of age-related nuclear cataract. BMC Ophthalmol. 18(1):93–104. doi:10.1186/s12886-018-0766-6.
   PubMed Web of Science ®Google Scholar
• Mei L, Yan H, Wang S, Guo CJ, Zheng XL, Yan B, Zhao J, Yang AG. Upregulation of miR-630 induced by oxidative damage resists cell migration through targeting ALCAM in human lens epithelium cells. Curr Eye Res. 2020;45(2):153–161. doi:10.1080/02713683.2019.1656748.
   PubMed Web of Science ®Google Scholar
• Neelam S, Brooks MM, Cammarata PR. Lenticular cytoprotection. Part 1: the role of hypoxia inducible factors-1alpha and -2alpha and vascular endothelial growth factor in lens epithelial cell survival in hypoxia. Mol Vis. 2013;19:1–15.
   PubMed Web of Science ®Google Scholar
• Bartel DP. MicroRNAs: target recognition and regulatory functions. Cell. 2009;136(2):215–233. doi:10.1016/j.cell.2009.01.002.
   PubMed Web of Science ®Google Scholar
• Betel D, Koppal A, Agius P, Sander C, Leslie C. Comprehensive modeling of microRNA targets predicts functional non-conserved and non-canonical sites. Genome Biol. 2010;11(8):R90. doi:10.1186/gb-2010-11-8-r90.
   PubMed Web of Science ®Google Scholar
• Mi H, Muruganujan A, Casagrande JT, Thomas PD. Large-scale gene function analysis with the PANTHER classification system. Nat Protoc. 2013;8(8):1551–1566. doi:10.1038/nprot.2013.092.
   PubMed Web of Science ®Google Scholar
• Chew MC, Lim LW, Tan CS. Multimedia interventions on the informed consent process for cataract surgery. Indian J Ophthalmol. 2014;62(11):1102–1103. doi:10.4103/0301-4738.146732.
   PubMed Web of Science ®Google Scholar
• West S. Epidemiology of cataract: accomplishments over 25 years and future directions. Ophthalmic Epidemiol. 2007;14(4):173–178. doi:10.1080/09286580701423151.
   PubMed Web of Science ®Google Scholar
• Nosalski R, Siedlinski M, Denby L, McGinnigle E, Nowak M, Cat AN, Ruiz LM, Cantini M, Skiba D, Wilk G, et al. 2020. T-cell-derived miRNA-214 mediates perivascular fibrosis in hypertension. Circ Res. 126(8):988–1003. doi:10.1161/CIRCRESAHA.119.315428.
   PubMed Web of Science ®Google Scholar
• Zeng J, Zhu L, Liu J, Zhu T, Xie ZH, Sun XO, Zhang H. Metformin protects against oxidative stress injury induced by ischemia/reperfusion via regulation of the lncRNA-H19/miR-148a-3p/Rock2 axis. Oxid Med Cell Longev. 2019;2019(12):8768327. doi:10.1155/2019/8768327.
   PubMed Web of Science ®Google Scholar
• Zhuang XY, Li RS, Maimaitijiang A, Liu RC, Yan FY, Hu HB, Gao XF, Shi HM. miR-221-3p inhibits oxidized low-density lipoprotein induced oxidative stress and apoptosis via targeting a disintegrin and metalloprotease-22. J Cell Biochem. 2019;120(4):6304–6314. doi:10.1002/jcb.27917.
   PubMed Web of Science ®Google Scholar
• Wolf L, Gao CS, Gueta K, Xie Q, Chevallier T, Podduturi NR, Sun J, Conte I, Zelenka PS, Padan RS, et al. Identification and characterization of FGF2-dependent mRNA: microRNA networks during lens fiber cell differentiation. G3. 2013;3(12):2239–2255. doi:10.1534/g3.113.008698.
   Web of Science ®Google Scholar
• Varma SD, Kovtun S, Hegde K, Yin J, Ramnath J. Effect of high sugar levels on miRNA expression: studies with galactosemic mice lenses. Mol Vis. 2012;18:1609–1618.
   PubMed Web of Science ®Google Scholar
• Varma SD, Kovtun S. Protective effect of caffeine against high sugar-induced transcription of microRNAs and consequent gene silencing: a study using lenses of galactosemic mice. Mol Vis. 2013;19:493–500.
   PubMed Web of Science ®Google Scholar
• Hoffmann A, Huang Y, Suetsugu-Maki R, Ringelberg CS, Tomlinson CR, Del Rio-Tsonis K, Tsonis PA. Implication of the miR-184 and miR-204 competitive RNA network in control of mouse secondary cataract. Mol Med. 2012;18:528–538. doi:10.2119/molmed.2011.00463.
   PubMed Web of Science ®Google Scholar
• Ito T, Yagi S, Yamakuchi M. MicroRNA-34a regulation of endothelial senescence. Biochem Biophys Res Commun. 2010;398(4):735–740. doi:10.1016/j.bbrc.2010.07.012.
   PubMed Web of Science ®Google Scholar
• Chou C-H, Chang N-W, Shrestha S, Hsu S-D, Lin Y-L, Lee W-H, Yang C-D, Hong H-C, Wei T-Y, Tu S-J, et al. miRTarBase 2016: updates to the experimentally validated miRNA-target interactions database. Nucleic Acids Res. 2016;44(D1):D239–D247. doi:10.1093/nar/gkv1258.
   PubMed Web of Science ®Google Scholar
• Bartel DP. MicroRNAs: genomics, biogenesis, mechanism, and function. Cell. 2004;116(2):281–297. doi:10.1016/s0092-8674(04)00045-5.
   PubMed Web of Science ®Google Scholar
• Evans C, Noirel J, Ow SY, Salim M, Pereira-Medrano AG, Couto N, Pandhal J, Smith D, Pham TK, Karunakaran E, et al. An insight into iTRAQ: where do we stand now. Anal Bioanal Chem. 2012;404(4):1011–1027. doi:10.1007/s00216-012-5918-6.
   PubMed Web of Science ®Google Scholar
• Li C, Xiong Q, Zhang J, Ge F, Bi LJ. Quantitative proteomic strategies for the identification of microRNA targets. Expert Rev Proteomics. 2012;9(5):549–559. doi:10.1586/epr.12.49.
   PubMed Web of Science ®Google Scholar
• Ou M, Zhang X, Dai Y, Gao JY, Zhu MS, Yang XC, Li YC, Yang T, Ding M. Identification of potential microRNA-target pairs associated with osteopetrosis by deep sequencing, iTRAQ proteomics and bioinformatics. Eur J Hum Genet. 2014;22(5):625–632. doi:10.1038/ejhg.2013.221.
   PubMed Web of Science ®Google Scholar
• Han T, Jiang S, Zheng H, Yin Q, Xie MY, Little MR, Yin X, Chen M, Song SJ, Beg AA, et al. Interplay between c-Src and the APC/C co-activator Cdh1 regulates mammary tumorigenesis. Nat Commun. 2019;10(1):3716. doi:10.1038/s41467-019-11618-7.
   PubMed Web of Science ®Google Scholar
• Zhang J, Wang S, Jiang B, Huang LH, Ji ZL, Li XT, Zhou HM, Han AD, Chen A, Wu YN, et al. c-Src phosphorylation and activation of hexokinase promotes tumorigenesis and metastasis. Nat Commun. 2017;8:13732. doi:10.1038/ncomms13732.
   PubMed Web of Science ®Google Scholar
• Huang RY, Wong MK, Tan TZ, Kuay KT, Ng AH, Chung VY, Chu YS, Matsumura N, Lai HC, Lee YF, et al. An EMT spectrum defines an anoikis-resistant and spheroidogenic intermediate mesenchymal state that is sensitive to e-cadherin restoration by a src-kinase inhibitor, saracatinib (AZD0530). Cell Death Dis. 2013;4(11):e915. doi:10.1038/cddis.2013.442.
   PubMed Web of Science ®Google Scholar
• Tu YY, Li LL, Qin B, Wu J, Cheng TY, Kang LH, Guan HJ. Long noncoding RNA glutathione peroxidase 3-antisense inhibits lens epithelial cell apoptosis by upregulating glutathione peroxidase 3 expression in age-related cataract. Mol Vis. 2019;25(25):734–744.
   PubMed Web of Science ®Google Scholar
• Reddy AT, Lakshmi SP, Banno A, Reddy RC. Role of GPx3 in PPARγ-induced protection against COPD-associated oxidative stress. Free Radic Biol Med. 2018;126:350–357. doi:10.1016/j.freeradbiomed.2018.08.014.
   PubMed Web of Science ®Google Scholar