Aerosol partitioning potential of bacteria presenting antimicrobial resistance from different stages of a small decentralized septic treatment system

References

• Abisado, R. G., S. Benomar, J. R. Klaus, A. A. Dandekar, and J. R. Chandler. 2018. Bacterial quorum sensing and microbial community interactions. MBio 9 (3):e02331. doi:10.1128/mBio.02331-17.
   PubMed Web of Science ®Google Scholar
• Amarasiri, M., D. Sano, and S. Suzuki. 2020. Understanding human health risks caused by antibiotic resistant bacteria (ARB) and antibiotic resistance genes (ARG) in water environments: Current knowledge and questions to be answered. Crit. Rev. Environ. Sci. Technol. 50 (19):2016–59. doi:10.1080/10643389.2019.1692611.
   Web of Science ®Google Scholar
• An, H. R., G. Mainelis, and L. White. 2006. Development and calibration of real-time PCR for quantification of airborne microorganisms in air samples. Atmos. Environ. 40 (40):7924–39. doi:10.1016/j.atmosenv.2006.07.020.
   Web of Science ®Google Scholar
• Bauer, A. W., W. M. M. Kirby, J. C. Sherris, and M. Turck. 1966. Antibiotic susceptibility testing by a standardized single disk method. Am. J. Clin. Pathol. 45 (4):493–6. doi:10.1093/ajcp/45.4_ts.493.
   PubMed Web of Science ®Google Scholar
• Beceiro, A., M. Tomás, and G. Bou. 2013. Antimicrobial resistance and virulence: A successful or deleterious association in the bacterial world? Clin. Microbiol. Rev. 26 (2):185–230. doi:10.1128/CMR.00059-12.
   PubMed Web of Science ®Google Scholar
• Bennett, P. M. 2008. Plasmid encoded antibiotic resistance: Acquisition and transfer of antibiotic resistance genes in bacteria. Br. J. Pharmacol. 153 (S1):S347–S357. doi:10.1038/sj.bjp.0707607.
   PubMed Web of Science ®Google Scholar
• BioMicrobics Inc. 2019. MBR BioBarrier brochure. https://biomicrobics.com/wp-content/uploads/BioBarrier_Brochure_email-2.pdf
   Google Scholar
• Brągoszewska, E., and I. Biedroń. 2018. Indoor air quality and potential health risk impacts of exposure to antibiotic resistant bacteria in an office room in southern Poland. Int. J. Environ. Res. Public Health. 15 (11):2604. doi:10.3390/ijerph15112604.
   PubMed Web of Science ®Google Scholar
• Caporaso, J. G., J. Kuczynski, J. Stombaugh, K. Bittinger, F. D. Bushman, E. K. Costello, N. Fierer, A. G. Peña, J. K. Goodrich, J. I. Gordon, et al. 2010. QIIME allows analysis of high-throughput community sequencing data. Nat. Methods. 7 (5):335–6. doi:10.1038/nmeth.f.303.
   PubMed Web of Science ®Google Scholar
• Centers for Disease Control and Prevention (CDC). 2019. Antibiotic resistance threats in the United States. Biggest Threats and Data. https://www.cdc.gov/drugresistance/biggest-threats.html
   Google Scholar
• Chen, H., and M. Zhang. 2013. Occurrence and removal of antibiotic resistance genes in municipal wastewater and rural domestic sewage treatment systems in eastern China. Environ. Int. 55:9–14. doi:10.1016/j.envint.2013.01.019.
   PubMed Web of Science ®Google Scholar
• Clearstream Wastewater Systems Inc. 2004. Owner’s manual. https://www.vdh.virginia.gov/content/uploads/sites/20/2016/05/Clearstream-Operation-and-Maintenance-Manual.pdf
   Google Scholar
• de Abreu, V. A. C., J. Perdigão, and S. Almeida. 2020. Metagenomic approaches to analyze antimicrobial resistance: An overview. Front. Genet. 11:575592. doi:10.3389/fgene.2020.575592.
   PubMed Web of Science ®Google Scholar
• Edgar, R. C. 2010. Search and clustering orders of magnitude faster than BLAST. Bioinformatics 26 (19):2460–1. doi:10.1093/bioinformatics/btq461.
   PubMed Web of Science ®Google Scholar
• EPA Annual Report. 2013. EPA-832-R-140006; Decentralized wastewater management program highlights. United States Environmental Protection Agency, p. 4. http://water.epa.gov/infrastructure/septic/
   Google Scholar
• Estrada-Perez, C. E., K. A. Kinney, J. P. Maestre, Y. A. Hassan, and M. D. King. 2018. Droplet distribution and airborne bacteria in an experimental shower unit. Water Res. 130:47–57. doi:10.1016/j.watres.2017.11.039.
   PubMed Web of Science ®Google Scholar
• Falgenhauer, L., O. Schwengers, J. Schmiedel, C. Baars, O. Lambrecht, S. Heß, T. U. Berendonk, J. Falgenhauer, T. Chakraborty, and C. Imirzalioglu. 2019. Multidrug-resistant and clinically relevant gram-negative bacteria are present in german surface waters. Front. Microbiol. 10:2779. doi:10.3389/fmicb.2019.02779.
   PubMed Web of Science ®Google Scholar
• Feldgarden, M., V. Brover, D. H. Haft, A. B. Prasad, D. J. Slotta, I. Tolstoy, G. H. Tyson, S. Zhao, C. H. Hsu, P. F. McDermott, et al. 2019. Validating the AMRFINder tool and resistance gene database by using antimicrobial resistance genotype-phenotype correlations in a collection of isolates. Antimicrob. Agents Chemother. 63 (11):1–19. doi:10.1128/AAC.00483-19.
   Web of Science ®Google Scholar
• Garrido-Cardenas, J. A., M. I. Polo-López, and I. Oller-Alberola. 2017. Advanced microbial analysis for wastewater quality monitoring: Metagenomics trend. Appl. Microbiol. Biotechnol. 101 (20):7445–58. doi:10.1007/s00253-017-8490-3.
   PubMed Web of Science ®Google Scholar
• Gaviria-Figueroa, A., E. C. Preisner, S. Hoque, C. E. Feigley, and R. S. Norman. 2019. Emission and dispersal of antibiotic resistance genes through bioaerosols generated during the treatment of municipal sewage. Sci. Total Environ. 686:402–12. doi:10.1016/j.scitotenv.2019.05.454.
   PubMed Web of Science ®Google Scholar
• Guo, J., J. Li, H. Chen, P. L. Bond, and Z. Yuan. 2017. Metagenomic analysis reveals wastewater treatment plants as hotspots of antibiotic resistance genes and mobile genetic elements. Water Res. 123:468–78. doi:10.1016/j.watres.2017.07.002.
   PubMed Web of Science ®Google Scholar
• Gupta, S. K., B. R. Padmanabhan, S. M. Diene, R. Lopez-Rojas, M. Kempf, L. Landraud, and J. M. Rolain. 2014. ARG-ANNOT, a new bioinformatic tool to discover antibiotic resistance genes in bacterial genomes. Antimicrob. Agents Chemother. 58 (1):212–20. doi:10.1128/AAC.01310-13.
   PubMed Web of Science ®Google Scholar
• Han, I., and K. Yoo. 2020. Metagenomic profiles of antibiotic resistance genes in activated sludge, dewatered sludge and bioaerosols. Water 12 (6):1516. doi:10.3390/w12061516.
   Web of Science ®Google Scholar
• Hendriksen, R. S., P. Munk, P. Njage, B. van Bunnik, L. McNally, O. Lukjancenko, T. Röder, D. Nieuwenhuijse, S. K. Pedersen, J. Kjeldgaard, et al. 2019. Global monitoring of antimicrobial resistance based on metagenomics analyses of urban sewage. Nat. Commun. 10 (1):1124. doi:10.1038/s41467-019-08853-3.
   PubMed Web of Science ®Google Scholar
• Hospodsky, D., J. Qian, W. W. Nazaroff, N. Yamamoto, K. Bibby, H. Rismani-Yazdi, and J. Peccia. 2012. Human occupancy as a source of indoor airborne bacteria. PLoS One 7 (4):e34867. doi:10.1371/journal.pone.0034867.
   PubMed Web of Science ®Google Scholar
• Hudzicki, J. 2014. Kirby-Bauer disk diffusion susceptibility test protocol. http://www.microbelibrary.org/component/resource/laboratory-test/3189-kirby-bauer-disk-diffusion-susceptibility-test-protocol
   Google Scholar
• Jia, B., Raphenya, A. R. Alcock, B. Waglechner, N. Guo, P. Tsang, K. K. Lago, B. A. Dave, B. M. Pereira, S. Sharma, et al. 2017. CARD 2017: Expansion and model-centric curation of the comprehensive antibiotic resistance database. Nucleic Acids Res. 45 (D1):D566–D573. doi:10.1093/nar/gkw1004.
   PubMed Web of Science ®Google Scholar
• Ju, F., K. Beck, X. Yin, A. Maccagnan, C. S. McArdell, H. P. Singer, D. R. Johnson, T. Zhang, and H. Bürgmann. 2019. Wastewater treatment plant resistomes are shaped by bacterial composition, genetic exchange, and upregulated expression in the effluent microbiomes. ISME J. 13 (2):346–60. doi:10.1038/s41396-018-0277-8.
   PubMed Web of Science ®Google Scholar
• King, M. D., and A. R. McFarland. 2012. Bioaerosol sampling with a wetted wall cyclone: Cell culturability and DNA integrity of Escherichia coli bacteria. Aerosol Sci. Technol. 46 (1):82–93. doi:10.1080/02786826.2011.605400.
   Web of Science ®Google Scholar
• Klappenbach, J., P. R. Saxman, J. R. Cole, and T. M. Schmidt. 2001. rrndb: The ribosomal RNA operon copy number database. Nucleic Acids Res. 29 (1):181–4. doi:10.1093/nar/29.1.181.
   PubMed Web of Science ®Google Scholar
• Lee, Z. M.-P., C. Bussema, and T. M. Schmidt. 2009. rrnDB: Documenting the number of rRNA and tRNA genes in bacteria and archaea. Nucleic Acids Res. 37 (suppl_1):D489–D493. doi:10.1093/nar/gkn689.
   PubMed Web of Science ®Google Scholar
• Leung, M. H. Y., X. Tong, K. O. Bøifot, D. Bezdan, D. J. Butler, D. C. Danko, J. Gohli, D. C. Green, M. T. Hernandez, F. J. Kelly, et al. 2021. Characterization of public transit air microbiome and resistome reveals geographical specificity. Microbiome 9 (1):112. doi:10.1186/s40168-021-010447.
   Web of Science ®Google Scholar
• Li, H., Z. Zhang, J. Duan, N. Li, B. Li, T. Song, M. F. Sardar, X. Lv, and C. Zhu. 2020. Electrochemical disinfection of secondary effluent from a wastewater treatment plant: Removal efficiency of ARGs and variation of antibiotic resistance in surviving bacteria. Chem. Eng. J. 392:123674. doi:10.1016/j.cej.2019.123674.
   Web of Science ®Google Scholar
• Li, J., W. Cheng, L. Xu, Y. Jiao, S. A. Baig, and H. Chen. 2016. Occurrence and removal of antibiotics and the corresponding resistance genes in wastewater treatment plants: Effluents’ influence to downstream water environment. Environ. Sci. Pollut. Res. 23 (7):6826–35. doi:10.1007/s11356-015-5916-2.
   PubMed Web of Science ®Google Scholar
• Li, Y., H. Liao, and H. Yao. 2019. Prevalence of antibiotic resistance genes in air-conditioning systems in hospitals, farms, and residences. Int. J. Environ. Res. Public Health 16 (5):683. doi:10.3390/ijerph16050683.
   Web of Science ®Google Scholar
• Liu, B., D. Zheng, Q. Jin, L. Chen, and J. Yang. 2019. VFDB 2019: A comparative pathogenomic platform with an interactive web interface. Nucleic Acids Res. 47 (D1):D687–D692. doi:10.1093/nar/gky1080.
   PubMed Web of Science ®Google Scholar
• Lozupone, C. A., M. Hamady, S. T. Kelley, and R. Knight. 2007. Quantitative and qualitative beta diversity measures lead to different insights into factors that structure microbial communities. Appl. Environ. Microbiol. 73 (5):1576–85. doi:10.1128/AEM.01996-06.
   PubMed Web of Science ®Google Scholar
• Magoč, T., and S. L. Salzberg. 2011. FLASH: Fast length adjustment of short reads to improve genome assemblies. Bioinformatics 27 (21):2957–63. doi:10.1093/bioinformatics/btr507.
   PubMed Web of Science ®Google Scholar
• McDonald, D., M. N. Price, J. Goodrich, E. P. Nawrocki, T. Z. DeSantis, A. Probst, G. L. Andersen, R. Knight, and P. Hugenholtz. 2012. An improved Greengenes taxonomy with explicit ranks for ecological and evolutionary analyses of bacteria and archaea. ISME J. 6 (3):610–8. doi:10.1038/ismej.2011.139.
   PubMed Web of Science ®Google Scholar
• McFarland, A. R., J. S. Haglund, M. D. King, S. Hu, M. S. Phull, B. W. Moncla, and Y. Seo. 2010. Wetted wall cyclones for bioaerosol sampling. Aerosol Sci. Technol. 44 (4):241–52. doi:10.1080/02786820903555552.
   Web of Science ®Google Scholar
• McLain, J. E., E. Cytryn, L. M. Durso, and S. Young. 2016. Culture-based methods for detection of antibiotic resistance in agroecosystems: Advantages, challenges, and gaps in knowledge. J. Environ. Qual. 45 (2):432–40. doi:10.2134/jeq2015.06.0317.
   PubMed Web of Science ®Google Scholar
• National Committee for Clinical Laboratory. 1999. Methods for determining bactericidal activity of antimicrobial agents: Approved guideline. Wayne, PA: NCCLS.
   Google Scholar
• Oberoi, A. S., Y. Jia, H. Zhang, S. K. Khanal, and H. Lu. 2019. Insights into the fate and removal of antibiotics in engineered biological treatment systems: A critical review. Environ. Sci. Technol. 53 (13):7234–64. doi:10.1021/acs.est.9b01131.
   PubMed Web of Science ®Google Scholar
• Osińska, A., E. Korzeniewska, M. Harnisz, E. Felis, S. Bajkacz, P. Jachimowicz, S. Niestępski, and I. Konopka. 2020. Small-scale wastewater treatment plants as a source of the dissemination of antibiotic resistance genes in the aquatic environment. J. Hazard. Mater. 381:121221. doi:10.1016/j.jhazmat.2019.121221.
   PubMed Web of Science ®Google Scholar
• Paulson, J. N., O. C. Stine, H. C. Bravo, and M. Pop. 2013. Differential abundance analysis for microbial marker-gene surveys. Nat. Methods 10 (12):1200–2. doi:10.1038/nmeth.2658.
   PubMed Web of Science ®Google Scholar
• Rizzo, L., C. Manaia, C. Merlin, T. Schwartz, C. Dagot, M. C. Ploy, I. Michael, and D. Fatta-Kassinos. 2013. Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: A review. Sci. Total Environ. 447:345–60. doi:10.1016/j.scitotenv.2013.01.032.
   PubMed Web of Science ®Google Scholar
• Roberts, M. C, 2017. Antibiotic-resistant environmental bacteria and their role as reservoirs in disease. In Modeling the transmission and prevention of infectious disease. Advances in environmental microbiology, ed. C. Hurst, vol. 4, 187–212. Cham: Springer. doi:10.1007/978-3-319-60616-3_7.
   Google Scholar
• Sharaby, Y., O. Nitzan, I. Brettar, M. G. Höfle, A. Peretz, and M. Halpern. 2019. Antimicrobial agent susceptibilities of Legionella pneumophila MLVA-8 genotypes. Sci. Rep. 9 (1):6138. PMID: 30992549; PMCID: PMC6468011. doi:10.1038/s41598-019-42425-1.
   PubMed Web of Science ®Google Scholar
• Sharma, V. K., X. Yu, T. J. McDonald, C. Jinadatha, D. D. Dionysiou, and M. Feng. 2019. Elimination of antibiotic resistance genes and control of horizontal transfer risk by UV-based treatment of drinking water: A mini review. Front. Environ. Sci. Eng. 13 (3):37. doi:10.1007/s11783-019-1122-7.
   Web of Science ®Google Scholar
• Stoddard, S. F., Smith, B. J. Hein, R. Roller, B. R. K, and Schmidt, T. M. 2015. rrnDB: Improved tools for interpreting rRNA gene abundance in bacteria and archaea and a new foundation for future development. Nucl. Acids Res. 43 (D1):D593–D598. doi:10.1093/nar/gku1201.
   PubMed Web of Science ®Google Scholar
• Texas A&M AgriLife Extension. 2020. Drip distribution. On-Site Sewage Facilities (OSSF). https://ossf.tamu.edu/drip-distribution/
   Google Scholar
• Wang, Y., and P.-Y. Qian. 2009. Conservative fragments in bacterial 16S rRNA genes and primer design for 16S ribosomal DNA amplicons in metagenomic studies. PLoS One 4 (10):e7401. doi:10.1371/journal.pone.0007401.
   PubMed Web of Science ®Google Scholar
• Yadav, S., and A. Kapley. 2021. Antibiotic resistance: Global health crisis and metagenomics. Biotechnol. Rep, 29:e00604. doi:10.1016/j.btre.2021.e00604.
   Google Scholar
• Zankari, E., H. Hasman, S. Cosentino, M. Vestergaard, S. Rasmussen, O. Lund, F. M. Aarestrup, and M. V. Larsen. 2012. Identification of acquired antimicrobial resistance genes. J. Antimicrob. Chemother. 67 (11):2640–4. doi:10.1093/jac/dks261.
   PubMed Web of Science ®Google Scholar
• Zankari, E., R. Allesøe, K. G. Joensen, L. M. Cavaco, O. Lund, and F. M. Aarestrup. 2017. PointFinder: A novel web tool for WGS-based detection of antimicrobial resistance associated with chromosomal point mutations in bacterial pathogens. J. Antimicrob. Chemother. 72 (10):2764–8. doi:10.1093/jac/dkx217.
   PubMed Web of Science ®Google Scholar
• Zhang, Y., C. F. Marrs, C. Simon, and C. Xi. 2009. Wastewater treatment contributes to selective increase of antibiotic resistance among Acinetobacter spp. Sci. Total Environ. 407 (12):3702–6. doi:10.1016/j.scitotenv.2009.02.013.
   PubMed Web of Science ®Google Scholar
• Zhao, J., B. Li, P. Lv, J. Hou, Y. Qiu, and X. Huang. 2022. Distribution of antibiotic resistance genes and their association with bacteria and viruses in decentralized sewage treatment facilities. Front. Environ. Sci. Eng. 16 (3):35. doi:10.1007/s11783-021-1469-4.
   PubMed Web of Science ®Google Scholar
• Zhou, C., Y. Yang, and A. Y. Jong. 1990. Mini-prep in ten minutes. Biotechniques. 8 (2):172–3.
   PubMed Web of Science ®Google Scholar