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Original Articles

Inactivation of virions in host particles in air using 222- and 254-nm UV: Dependence of shielding on particle size and UV wavelength

ORCID Icon, ORCID Icon &
Pages 512-535 | Received 06 Sep 2023, Accepted 25 Jan 2024, Published online: 08 Mar 2024

References

  • ACGIH. 2023. TLVs and BEIs based on the documentation of the threshold limit values for chemical substances and physical agents and biological exposure indices. Cincinatti, OH: ACGIH. ISBN: 978-160-726157-5. 158–64.
  • Allen, J. G., P. MacNaughton, J. G. C. Laurent, S. S. Flanigan, E. S. Eitland, and J. D. Spengler. 2015. Green buildings and health. Curr. Environ. Health Rep. 2 (3):250–8. doi: 10.1007/s40572-015-0063-y.
  • Almstrand, A.-C., B. Bake, E. Ljungström, P. Larsson, A. Bredberg, E. Mirgorodskaya, and A.-C. Olin. 2010. Effect of airway opening on production of exhaled particles. J. Appl. Physiol. (1985) 108 (3):584–8. doi: 10.1152/japplphysiol.00873.2009.
  • Almstrand, A.-C., M. Josefson, A. Bredberg, J. Lausmaa, P. Sjövall, P. Larsson, and A.-C. Olin. 2012. TOF-SIMS analysis of exhaled particles from patients with asthma and healthy controls. Eur. Respir. J. 39 (1):59–66. doi: 10.1183/09031936.00195610.
  • Anderson, D. J., R. W. Moehring, D. J. Weber, S. S. Lewis, L. F. Chen, J. C. Schwab, P. Becherer, M. Blocker, P. F. Triplett, L. P. Knelson, et al. 2018. Effectiveness of targeted enhanced terminal room disinfection on hospital-wide acquisition and infection with multidrug-resistant organisms and Clostridium difficile: A secondary analysis of a multicentre cluster randomized controlled trial with crossover design (BETR Disinfection). Lancet. Infect. Dis. 18 (8):845–53. doi: 10.1016/S1473-3099(18)30278-0.
  • Arcanjo, A., J. Logullo, C. C. B. Menezes, T. C. de Souza Carvalho Giangiarulo, M. C. Dos Reis, G. M. M. de Castro, Y. da Silva Fontes, A. R. Todeschini, L. Freire-de-Lima, D. Decoté-Ricardo, et al. 2020. The emerging role of neutrophil extracellular traps in severe acute respiratory syndrome coronavirus 2 (COVID‑19). Sci. Rep. 10 (1):19630. doi: 10.1038/s41598-020-76781-0.
  • Asadi, S., A. S. Wexler, C. D. Cappa, S. Barreda, N. M. Bouvier, and W. D. Ristenpart. 2019. Aerosol emission and superemission during human speech increase with voice loudness. Sci. Rep. 9 (1):2348. doi: 10.1038/s41598-019-38808-z.
  • ASHRAE. 2019. “Ultraviolet air and surface treatment,” Ch. 62. 2019 ASHRAE handbook-HVAC applications. Peachtree Corners, GA: ASHRAE.
  • Baniassadi, A., J. Heusinger, P. I. Gonzalez, S. Weber, and H. W. Samuelson. 2022. Co-benefits of energy efficiency in residential buildings. Energy 238:121768. doi: 10.1016/j.energy.2021.121768.
  • Bax, A., Y. Shen, Y. T. Kakeshpour, and K. P. Fennelly. 2022. Snoring may transmit infectious aerosols from the upper to the lower respiratory tract. Med. Hypotheses 168:110966. doi: 10.1016/j.mehy.2022.110966.
  • Behndig, A. F., E. Mirgorodskaya, A. Blomberg, and A. C. Olin. 2019. Surfactant Protein A in particles in exhaled air (PExA), bronchial lavage and bronchial wash - a methodological comparison. Respir. Res. 20 (1):214. doi: 10.1186/s12931-019-1172-1.
  • Bender, E. 2022. Safety is in the air. Nature 610 (7933):S46–S47. doi: 10.1038/d41586-022-03360-w.
  • Bicer, E. M. 2015. Compositional characterisation of human respiratory tract lining fluids for the design of disease specific simulants. PhD diss., Kings College. https://kclpure kcl.ac.uk/portal/
  • Blatchley, E. R., Ill, D. J. Brenner, H. Claus, T. E. Cowan, K. G. Linden, Y. Liu, T. Mao, S.-J. Park, P. J. Piper, R. M. Simons, et al. 2023. Far UV-C radiation: An emerging tool for pandemic control. Crit. Rev. Environ. Sci. Technol. 53 (6):733–53. doi: 10.1080/10643389.2022.2084315.
  • Bredberg, A., J. Gobom, A.-C. Almstrand, P. Larsson, K. Blennow, A. C. Olin, and E. Mirgorodskaya. 2012. Exhaled endogenous particles contain lung proteins. Clin. Chem. 58 (2):431–40. doi: 10.1373/clinchem.2011.169235.
  • Brice, B. A., and M. L. Swain. 1945. Ultraviolet absorption method for the determination of polyunsaturated constituents in fatty materials. J. Opt. Soc. Am. 35 (8):532–44. doi: 10.1364/JOSA.35.000532.
  • Buonanno, M., B. Ponnaiya, D. Welch, M. Stanislauskas, G. Randers-Pehrson, L. Smilenov, F. D. Lowy, D. M. Owen, and D. J. Brenner. 2017. Germicidal efficacy and mammalian skin safety of 222-nm UV light. Radiat. Res. 187 (4):483–91. doi: 10.1667/RR0010CC.1.
  • Buonanno, M., D. Welch, I. Shuryak, and D. J. Brenner. 2020. Far-UVC light (222 nm) efficiently and safely inactivates airborne human coronaviruses. Sci. Rep. 10 (1):10285. doi: 10.1038/s41598-020-67211-23.
  • Chen, A., T. Wessler, K. Daftari, K. Hinton, R. C. Boucher, R. Pickles, R. Freeman, S. K. Lai, and M. G. Forest. 2022. Modeling insights into SARS-CoV-2 respiratory tract infections prior to immune protection. Biophys. J. 121 (9):1619–31. doi: 10.1016/j.bpj.2022.04.003.
  • Chen, R. Z., S. A. Craik, and J. R. Bolton. 2009. Comparison of the action spectra and relative DNA absorbance spectra of microorganisms: Information important for the determination of germicidal fluence (UV dose) in an ultraviolet disinfection of water. Water Res. 43 (20):5087–96. doi: 10.1016/j.watres.2009.08.03296.
  • Chowdhury, D. Q., P. W. Barber, and S. C. Hill. 1992. Energy-density distribution inside large non-absorbing spheres by using Mie theory and geometrical optics. Appl. Opt. 31 (18):3518–23. doi: 10.1364/AO.31.003518.
  • Coleman, K. K., D. J. W. Tay, K. S. Tan, S. W. X. Ong, T. S. Than, M. H. Koh, Y. Q. Chin, H. Nasir, T. M. Mak, J. J. H. Chu, et al. 2022. Viral load of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) in respiratory aerosols emitted by patients with Coronavirus Disease 2019 (COVID-19) while breathing, talking, and singing. Clin. Infect. Dis. 74 (10):1722–8. doi: 10.1093/cid/ciab691.
  • Dey, S., D. Sain, and S. Goswami. 2014. Naphthyridine based fluorescent receptors for the recognition of uric acid. RSC Adv. 4 (1):428–33. doi: 10.1039/C3RA45197F.
  • Doughty, D. C., S. C. Hill, and D. W. Mackowski. 2021. Viruses such as SARS-CoV-2 can be partially shielded from UV radiation when in particles generated by sneezing or coughing: Numerical simulations. J. Quant. Spectrosc. Radiat. Transf. 262:107489. doi: 10.1016/j.jqsrt.2020.107489.
  • Eadie, E., W. Hiwar, L. Fletcher, E. Tidswell, P. O'Mahoney, M. Buonanno, D. Welch, C. S. Adamson, D. J. Brenner, C. Noakes, et al. 2022. Far‑UVC (222 nm) efficiently inactivates an airborne pathogen in a room‑sized chamber. Sci. Rep. 12 (1):4373. doi: 10.1038/s41598-022-08462-z.
  • Edgar, M., C. Dawes, and D. D. O’Mullane. 2012. Saliva and oral health. 4th ed. London: Stephen Hancocks, Ltd.; 2012. Table 1.2 and references therein, and Fig. 5.4.
  • Evans, C. M., and J. S. Koo. 2009. Airway mucus: The good, the bad, the sticky. Pharmacol. Ther. 121 (3):332–48. doi: 10.1016/j.pharmthera.2008.11.001.
  • Eversole, J. D., C. S. Scotto, M. B. Hart, J. Czege, J. S. Kesavan, V. K. Rastogi, D. R. McGrady, T. Ingersoll, J. B. Cabalo, S. C. Hill, et al. 2023. Prediction of aerosol viability from experiments on surfaces (PAVES): Program summary report, 142. NRL formal report, Naval Research Laboratory, Washington, DC.
  • Fahy, J. V., and B. F. Dickey. 2010. Airway mucus function and dysfunction. N Engl. J. Med. 363 (23):2233–47. doi: 10.1056/NEJMra0910061.
  • Fahy, J. V., D. J. Steiger, J. Liu, C. B. Basbaum, W. E. Finkbeiner, and H. A. Boushey. 1993. Markers of mucus secretion and DNA levels in induced sputum from asthmatic and from healthy subjects. Am. Rev. Respir. Dis. 147 (5):1132–7. doi: 10.1164/ajrccm/147.5.1132.
  • Fennelly, K. P., J. W. Martyny, K. E. Fulton, I. M. Orme, D. M. Cave, and L. B. Heifets. 2004. Cough-generated aerosols of Mycobacterium tuberculosis a new method to study infectiousness. Am. J. Respir. Crit. Care Med. 169 (5):604–9. doi: 10.1164/rccm.200308-1101OC.
  • Fischer, R. J., D. H. Morris, N. van Doremalen, S. Sarchette, M. J. Matson, T. Bushmaker, C. K. Yinda, S. N. Seifert, A. Gamble, B. N. Williamson, et al. 2020. Effectiveness of N95 respirator decontamination and reuse against SARS-CoV-2 virus. Emerg. Infect. Dis. 26 (9):2253–5. doi: 10.3201/eid2609.201524.
  • Fisher, E. M., A. W. Richardson, S. D. Harpest, K. C. Hofacre, and R. E. Shaffer. 2012. Reaerosolization of MS2 bacteriophage from an N95 filtering facepiece respirator by simulated coughing. Ann. Occup. Hyg. 56 (3):315–25. doi: 10.1093/annhyg/mer101.
  • Flatau, P. J., K. A. Fuller, and D. W. Mackowski. 1993. Scattering by two spheres in contact: Comparisons between discrete-dipole approximation and modal analysis. Appl. Opt. 32 (18):3302–5. doi: 10.1364/AO.32.003302.
  • Fukui, T., T. Niikura, T. Oda, Y. Kumabe, H. Ohashi, M. Sasaki, T. Igarashi, M. Kunisada, N. Yamano, K. Oe, et al. 2020. Exploratory clinical trial on the safety and bactericidal effect of 222-nm ultraviolet C irradiation in healthy humans. PLoS One 15 (8):e0235948. doi: 10.1371/journal.pone.0235948.
  • Geisler, T. S., M. V. Majji, J. S. Kesavan, V. J. Alstadt, E. S. G. Shaqfeh, and G. Iaccarino. 2019. Simulation of microparticle inhalation in rhesus monkey airways. Phys. Rev. Fluids 4 (8):083101. doi: 10.1103/PhysRevFluids.4.083101.
  • Goldfarb, A. R. 1953. Absorption spectrum of the peptide bond: Influence of chain length. J. Biol. Chem. 201 (1):317–20. doi: 10.1016/S0021-9258(18)71372-0.
  • Gregson, F. K. A., N. A. Watson, C. M. Orton, A. E. Haddrell, L. P. McCarthy, T. J. R. Finnie, N. Gent, G. C. Donaldson, P. L. Shah, J. D. Calder, et al. 2021. Comparing aerosol concentrations and particle size distributions generated by singing, speaking, and breathing. Aerosol Sci. Technol. 55 (6):681–91. doi: 10.1080/02786826.2021.188354.
  • Haro, K., M. Ogawa, M. Saito, K. Kusuhara, and K. Fukuda. 2020. Bacterial composition of nasal discharge in children based on highly accurate 16S rRNA gene sequencing analysis. Sci. Rep. 10 (1):20193. doi: 10.1038/s41598-020-77271-z.
  • Harrison, J., B. Saccente-Kennedy, C. M. Orton, L. P. McCarthy, J. Archer, H. E. Symons, A. Szczepanska, N. A. Watson, W. J. Browne, B. Moseley, et al. 2023. Emission rates, size distributions, and generation mechanism of oral respiratory droplets. Aerosol Sci. Technol. 57 (3):187–99. doi: 10.1080/02786826.2022.2158778.
  • Hassoun, M., P. G. Royall, M. Parry, R. D. Harvey, and B. Forbes. 2018. Design and development of a biorelevant simulated human lung fluid. J. Drug Deliv. Sci. Technol. 47:485–91. doi: 10.1016/j.jddst.2018.08.006.
  • Hawkins, G. R., I. Zipkin, and L. M. Marshall. 1963. Determination of uric acid, tyrosine, tryptophan, and protein in whole human parotid saliva by ultraviolet absorption spectrophotometry. J. Dent. Res. 42 (4):1015–22. doi: 10.1177/00220345630420040301.
  • He, X. M., and D. C. Carter. 1992. Atomic structure and chemistry of human serum albumin. Nature 358 (6383):209–15. doi: 10.1038/358209a0.
  • Heilingloh, C. S., U. W. Aufderhorst, L. Schipper, U. Dittmer, O. Witzke, D. Yang, X. Zheng, K. Sutter, M. Trilling, M. Alt, et al. 2020. Susceptibility of SARS-CoV-2 to UV irradiation. Am. J. Infect. Control. 48 (10):1273–5. doi: 10.1016/j.ajic.2020.07.031.
  • Hessling, M., R. Haag, N. Sieber, and P. Vatter. 2021. The impact of far-UVC radiation (200–230 nm) on pathogens, cells, skin, and eyes – a collection and analysis of a hundred years of data. GMS Hyg. Infect. Control. 16:2196–5226. doi: 10.3205/dgkh000378.
  • Hill, D. B., B. Button, M. Rubinstein, and R. C. Boucher. 2022. Physiology and pathology of human airway mucus. Physiol. Rev. 102 (4):1757–836. doi: 10.1152/physrev.00004.2021.
  • Hill, D. B., P. A. Vasquez, J. Mellnik, S. A. McKinley, A. Vose, F. Mu, A. G. Henderson, S. H. Donaldson, N. E. Alexis, R. C. Boucher, et al. 2014. A biophysical basis for mucus solids concentration as a candidate biomarker for airways disease. PLoS One 9 (2):e87681. doi: 10.1371/journal.pone.0087681.
  • Hill, S. C., D. C. Doughty, and D. W. Mackowski. 2022. Absorption of ultraviolet radiation in bacterial spores in clusters in air and on surfaces: Model calculations using the Multi-sphere T-Matrix Method. J. Quant. Spectrosc. Radiat. Transf. 293:108383. doi: 10.1016/j.jqsrt.2022.108383.
  • Hill, S. C., D. C. Doughty, D. W. Mackowski, V. Rastogi, J. D. Eversole, D. McGrady, F. Handler, and J. Kesavan. 2023. Enhanced survival fractions of UV-irradiated spores in clusters on surfaces in air: Measured and mathematically modeled results at 254-nm. Aerosol Sci. Technol. 57:487–507. doi: 10.1080/02786826.2023.2186213.
  • Hill, S. C., D. W. Mackowski, and D. C. Doughty. 2021. Shielding of viruses such as SARS-Cov-2 from ultraviolet radiation in particles generated by sneezing or coughing: Numerical simulations of survival fractions. J. Occup. Environ. Hyg. 18 (8):394–408. doi: 10.1080/15459624.2021.1939877.
  • Hill, S. C., G. Videen, W. Sun, and Q. Fu. 2001. Scattering and internal fields of a microsphere that contains a saturable absorber: Finite-difference time-domain simulations. Appl. Opt. 40 (30):5487–94. doi: 10.1364/AO.40.005487.
  • Hill, S. C., C. C. Williamson, D. C. Doughty, Y. L. Pan, J. L. Santarpia, and H. H. Hill. 2015. Size-dependent fluorescence of bioaerosols: Mathematical model using fluorescing and absorbing molecules in bacteria. J. Quant. Spectrosc. Radiat. Transf 157:54–70. doi: 10.1016/j.jqsrt.2015.01.011.
  • Hou, Y. J., K. Okuda, C. E. Edwards, D. R. Martinez, T. Asakura, K. H. Dinnon, T. Kato, R. E. Lee, B. L. Yount, T. M. Mascenik, et al. 2020. SARS-CoV-2 reverse genetics reveals a variable infection gradient in the respiratory tract. Cell 182 (2):429–46.e14. doi: 10.1016/j.cell.2020.05.042.
  • Huang, N., P. Pérez, T. Kato, Y. Mikami, K. Okuda, R. C. Gilmore, C. D. Conde, B. Gasmi, S. Stein, M. Beach, et al. 2021. SARS-CoV-2 infection of the oral cavity and saliva. Nat. Med. 27 (5):892–903. doi: 10.1038/s41591-021-01296-8.
  • Hussain-Alkhateeb, L., B. Bake, M. Holm, O. Emilsson, E. Mirgorodskaya, and A.-C. Olin. 2021. Novel non-invasive particles in exhaled air method to explore the lining fluid of small airways—a European population-based cohort study. BMJ Open Resp. Res. 8 (1):e000804. doi: 10.1136/bmjresp-2020-000804.
  • Jaffe, H. H., and M. Orchin. 1962. Theory and application of ultraviolet spectroscopy, 62–151181. New York, NY: John Wiley and Sons. ISBN-13 978–0471436744.
  • Janssen, W. J., A. L. Stefanski, B. S. Bochner, and C. M. Evans. 2016. Control of lung defence by mucins and macrophages: Ancient defence mechanisms with modern functions. Eur. Respir. J. 48 (4):1201–14. doi: 10.1183/13993003.00120-2015.
  • Johnson, G. R., and L. Morawska. 2009. The mechanism of breath aerosol formation. J. Aerosol Med. Pulm. Drug Deliv. 22 (3):229–37. doi: 10.1089/jamp.2008.0720.
  • Kesavan, J., D. Schepers, J. Bottiger, and J. Edmonds. 2014. UV-C decontamination of aerosolized and surface-bound single spores and bioclusters. Aerosol Sci. Technol. 48 (4):450–7. doi: 10.1080/02786826.2014.889276.
  • Konečná, B., A. Gaál Kovalčíková, A. Pančíková, B. Novák, E. Kovaľová, P. Celec, and Ľ. Tóthová. 2020. Salivary extracellular DNA and DNase activity in periodontitis. Appl. Sci. 10 (21):7490. doi: 10.3390/app10217490.
  • Kowalski, W. 2009. Ultraviolet germicidal irradiation handbook: UVGI for air and surface disinfection. New York, NY: Springer. doi: 10.1007/978-3-642-01999-9.
  • Kozlowski, L. P. 2016. Proteome-pl: Proteome isoelectric point. Nucleic Acids Res. 45 (D1):D1112–D1116. doi: 10.1093/nar/gkw978.
  • Kratochvil, M. J., G. Kaber, S. Demirdjian, P. C. Cai, E. B. Burgener, N. Nagy, G. L. Barlow, M. Popescu, M. R. Nicolls, M. G. Ozawa, et al. 2022. Biochemical, biophysical, and immunological characterization of respiratory secretions in severe SARS-CoV-2 (COVID-19) infections. JCI Insight 7 (12):e152629. doi: 10.1172/jci.insight.152629.
  • Kumar, A., W. Terakosolphan, M. Hassoun, K.-K. Vandera, A. Novicky, R. Harvey, P. G. Royall, E. M. Bicer, J. Eriksson, K. Edwards, et al. 2017. A biocompatible synthetic lung fluid based on human respiratory tract lining fluid composition. Pharm. Res. 34 (12):2454–65. doi: 10.1007/s11095-017-2169-4.
  • Kushalnagar, P., C. C. Chow, and A. Bax. 2021. Self-infection with speech aerosol may contribute to COVID-19 severity. J. Intern. Med. 290 (6):1275–7. doi: 10.1111/joim.13370.
  • Lachowicz-Scroggins, M. E., E. M. Dunican, A. R. Charbit, W. Raymond, M. R. Looney, M. C. Peters, E. D. Gordon, P. G. Woodruff, E. Lefrançais, B. R. Phillips, et al. 2019. Extracellular DNA, neutrophil extracellular traps, and inflammasome activation in severe asthma. Am. J. Respir. Crit. Care Med. 199 (9):1076–85. doi: 10.1164/rccm.201810-1869OC.
  • Lai, J., K. K. Coleman, S. H S. Tai, J. German, F. Hong, B. Albert, Y. Esparza, A. K. Srikakulapu, M. Schanz, I. S. Maldonado, et al. 2023. Exhaled breath aerosol shedding of highly transmissible versus prior severe acute respiratory syndrome coronavirus 2 variants. Clin. Infect. Dis. 76 (5):786–94. doi: 10.1093/cid/ciac846.
  • Larsson, P., B. Bake, A. Wallin, O. Hammar, A. C. Almstrand, M. Lärstad, E. Ljungström, E. Mirgorodskaya, and A. C. Olin. 2017. The effect of exhalation flow on endogenous particle emission and phospholipid composition. Respir. Physiol. Neurobiol. 243:39–46. doi: 10.1016/j.resp.2017.05.003.
  • Larsson, P., M. Lärstad, B. Bake, O. Hammar, A. Bredberg, A. C. Almstrand, E. Mirgorodskaya, and A. C. Olin. 2015. Exhaled particles as markers of small airway inflammation in subjects with asthma. Clin. Physiol. Funct. Imaging 37 (5):489–97. doi: 10.1111/cpf.12323.
  • Leung, N. H., D. K. W. Chu, E. Y. C. Shiu, K. H. Chan, J. J. McDevitt, B. J. P. Hau, H.-L. Yen, Y. Li, D. K. M. I. D. K., J. S. M. Peiris, W. H. Seto, et al. 2020. Respiratory virus shedding in exhaled breath and efficacy of face masks. Nat. Med. 26 (5):676–80. doi: 10.1038/s41591-020-0946-9org/10.1038/s41591-020-0843-2.
  • Lindsley, W. G., T. L. McClelland, D. T. Neu, S. B. Martin, K. R. Mead, R. E. Thewlis, and J. D. Noti. 2018. Ambulance disinfection using Ultraviolet Germicidal Irradiation (UVGI): Effects of fixture location and surface reflectivity. J. Occup. Environ. Hyg. 15 (1):1–12. doi: 10.1080/15459624.2017.1376067.
  • Linssen, R. S., G. Chai, J. Ma, A. B. Kummarapurugu, J. B. M. van Woensel, R. A. Bem, L. Kaler, G. A. Duncan, L. Zhou, B. K. Rubin, et al. 2021. Neutrophil extracellular traps increase airway mucus viscoelasticity and slow mucus particle transit. Am. J. Respir. Cell Mol. Biol. 64 (1):69–78. doi: 10.1165/rcmb.2020-0168OC.
  • Liuzzo Scorpo, M., G. Ferrante, and S. La Grutta. 2021. An overview of asthma and COVID-19: Protective factors against SARS-COV-2 in pediatric patients. Front. Pediatr. 9:661206. doi: 10.3389/fped.2021.661206.
  • Lu, J., J. Gu, K. Li, K. Xu, W. Su, Z. Lai, D. Zhou, C. Yu, B. Xu, and Z. Yang. 2020. COVID-19 outbreak associated with air conditioning in restaurant, Guangzhou, China, 2020. Emerg. Infect. Dis. 26 (7):1628–31. doi: 10.3201/eid2607.200764.
  • Mackowski, D. W. 1991. Analysis of radiative scattering for multiple sphere configurations. Proc. R. Soc. Lond. Ser. A 433:599–614. doi: 10.1098/rspa.1991.0066.
  • Mackowski, D. W. 2008. Exact solution for the scattering and absorption properties of sphere clusters on a plane surface. J. Quant. Spectrosc. Radiat. Transf. 109 (5):770–88. doi: 10.1016/j.jqsrt.2007.08.024.
  • Mackowski, D. W. 2014. A general superposition solution for electromagnetic scattering by multiple spherical domains of optically active media. J. Quant. Spectrosc. Radiat. Transf. 133:264–70. doi: 10.1016/j.jqsrt.2013.08.012.
  • Mackowski, D. W., and M. I. Mishchenko. 2011. A multiple sphere T-matrix Fortran code for use on parallel computer clusters. J. Quant. Spectrosc. Radiat. Transf. 112 (13):2182–92. doi: 10.1016/j.jqsrt.2011.02.019.
  • Mackowski, D. W., and M. I. Mishchenko. 2013. Direct simulation of extinction in a slab of spherical particles. J. Quant. Spectrosc. Radiat. Transf. 109 (5):770–88. doi: 10.1016/j.jqsrt.2013.02.008.
  • Matsui, H., M. W. Verghese, M. Kesimer, U. E. Schwab, S. H. Randell, J. K. Sheehan, B. R. Grubb, and R. C. Boucher. 2005. Reduced three-dimensional motility in dehydrated airway mucus prevents neutrophil capture and killing bacteria on airway epithelial surfaces. J. Immunol. 175 (2):1090–9. doi: 10.4049/jimmunol.175.2.1090.
  • Merhi, T., O. Atasi, C. Coetsier, B. Lalanne, and K. Roger. 2022. Assessing suspension and infectivity times of virus-loaded aerosols involved in airborne transmission. Proc. Natl. Acad. Sci. USA. 119 (32):1–10. doi: 10.1073/pnas.2204593119.
  • Miller, S. L., W. W. Nazaroff, J. L. Jimenez, A. Boerstra, G. Buonanno, S. J. Dancer, J. Kurnitski, L. C. Marr, L. Morawska, and C. Noakes. 2020. Transmission of SARS-CoV-2 by inhalation of respiratory aerosol in the Skagit Valley Chorale superspreading event. Indoor Air. 31 (2):314–23. doi: 10.1111/ina.12751.
  • Milton, D. K., M. P. Fabian, B. J. Cowling, M. L. Grantham, and J. J. McDevitt. 2013. Influenza virus aerosols in human exhaled breath: Particle size, culturability, and effect of surgical masks. PLoS Pathog. 9 (3):e1003205. doi: 10.1371/journal.ppat.1003205.
  • Morawska, L., J. Allen, W. Bahnfleth, P. M. Bluyssen, A. Boerstra, G. Buonanno, J. Cao, S. J. Dancer, A. Floto, F. Franchimon, et al. 2021. A paradigm shift to combat indoor respiratory infection. Science 372 (6543):689–91. doi: 10.1126/science.abg202.
  • Morawska, L., G. Buonanno, A. Mikszewski, and L. Stabile. 2022. The physics of respiratory particle generation, fate in the air, and inhalation. Nat. Rev. Phys. 4 (11):723–34. doi: 10.1038/s42254-022-00506-7.
  • Morawska, L., G. R. Johnson, Z. D. Ristovski, M. Hargreaves, K. Mengersen, S. Corbett, C. Y. H. Chao, Y. Li, and D. Katoshevski. 2009. Size distribution and sites of origin of droplets expelled from the human respiratory tract during expiratory activities. J. Aerosol Sci. 40 (3):256–69. doi: 10.1016/j.jaerosci.2008.11.002.
  • Mphaphlele, M., A. S. Dharmadhikari, P. A. Jensen, S. N. Rudnick, T. H. van Reenen, M. A. Pagano, W. Leuschner, T. A. Sears, S. P. Milonova, M. van der Walt, et al. 2015. Institutional tuberculosis transmission controlled trial of upper room ultraviolet air disinfection: A basis for new dosing guidelines. Am. J. Respir. Crit. Care Med. 192 (4):477–84. doi: 10.1164/rccm.201501-0060OC.
  • Nardell, E. A. 2016. Indoor environmental control of tuberculosis and other airborne infections. Indoor Air. 26 (1):79–87. doi: 10.1111/ina.12232.
  • Nardell, E. A. 2021. Air disinfection for airborne infection control with a focus on COVID-19: Why Germicidal UV is essential. Photochem. Photobiol. 97 (3):493–7. doi: 10.1111/php.13421.
  • Nardell, E. A., S. J. Bucher, P. W. Brickner, C. Wang, R. L. Vincent, K. Becan-McBride, M. A. James, M. Michael, and J. D. Wright. 2008. Safety of upper-room ultraviolet germicidal air disinfection for room occupants: Results from the tuberculosis ultraviolet shelter study. Public Health Rep. 123 (1):52–60. doi: 10.1177/003335490812300108.
  • Nardell, E., P. Lederer, H. Mishra, R. Nathavitharana, and G. Theron. 2020. Cool but dangerous: How climate change is increasing the risk of airborne infections. Indoor Air. 30 (2):195–7. doi: 10.1111/ina.12608.
  • Nasr, B., G. Ahmadi, A. R. Ferro, and S. Dhaniyala. 2019. Overview of mechanistic particle resuspension models: Comparison with compilation of experimental data. J. Adhes. Sci. Technol. 33 (24):2631–60. doi: 10.1080/01694243.2019.1650989.
  • Okuda, K., G. Chen, D. B. Subramani, M. Wolf, R. C. Gilmore, T. Kato, G. Radicioni, M. Kesimer, M. Chua, H. Dang, et al. 2019. Localization of secretory mucins MUC5AC and MUC5B in normal/healthy human airways. Am. J. Respir. Crit. Care Med. 199 (6):715–27. doi: 10.1164/rccm.201804-0734OC.
  • Pandit, P., J. Cooper-White, and C. Punyadeera. 2013. High-yield RNA-extraction method for saliva. Clin. Chem. 59 (7):1118–22. doi: 10.1373/clinchem.2012.197863.
  • Papineni, R. S., and F. S. Rosenthal. 1997. The size distribution of droplets in the exhaled breath of healthy human subjects. J. Aerosol Med. 10 (2):105–16. doi: 10.1089/jam.1997.10.105.
  • Park, S., R. Mistrick, and D. Rim. 2022. Performance of upper-room ultraviolet germicidal irradiation (UVGI) system in learning environments: Effects of ventilation rate, UV fluence rate, and UV radiating volume. Sustain. Cities Soc. 85:104048. doi: 10.1016/j.scs.2022.104048.
  • Park, S., R. Mistrick, W. Sitzabee, and D. Rim. 2023. Effect of ventilation strategy on performance of upper-room ultraviolet germicidal irradiation (UVGI) system in a learning environments. Sci. Total Environ. 85:104048. doi: 10.1016/j.scs.2022.104048.
  • Patton, J. S., and P. R. Byron. 2007. Inhaling medicines: Delivering drugs to the body through the lungs. Nat. Rev. Drug Discov. 6 (1):67–74. doi: 10.1038/nrd2153.
  • Peñaloza, C. H., A. Wood, E. Eadie, C. Noakes, M. F. King, and K. Wood. 2023. Validated computational modelling to evaluate spatial inactivation of airborne pathogens by Far‐UVC irradiation. Photochem. Photobiol. 99:1502–1502. doi: 10.1111/php.13807.
  • Péré, H., I. Podglajen, M. Wack, E. Flamarion, T. Mirault, G. Goudot, C. Hauw-Berlemont, L. Le, E. Caudron, S. Carrabin, et al. 2020. Nasal swab sampling for SARS-CoV-2: A convenient alternative in times of nasopharyngeal swab shortage. J. Clin. Microbiol. 58 (6):e00721-20. doi: 10.1128/JCM.00721-20.
  • Pinto, A. L., R. K. Rai, J. C. Brown, P. Griffin, J. R. Edgar, J. Shah, A. Singanayagam, C. Hogg, W. S. Barclay, C. E. Futter, et al. 2022. Ultrastructural insight into SARS-CoV-2 entry and budding in human airway epithelium. Nat. Commun. 13 (1):1609. doi: 10.1038/s41467-022-29255-y.
  • Poehls, U. G., C. C. Hack, A. B. Ekici, M. W. Beckmann, P. A. Fasching, M. Ruebner, and H. Huebner. 2018. Saliva samples as a source of DNA for high throughput genotyping: An acceptable and sufficient means in improvement of risk estimation throughout mammographic diagnostics. Eur. J. Med. Res. 23 (1):20. doi: 10.1186/s40001-018-0318-9.
  • Pöhlker, M. L., C. Pöhlker, O. O. Krüger, J.D. Förster, T. Berkemeier, W. Elbert, J. Fröhlich-Nowoisky, U. Pöschl, G. Bagheri, E. Bodenschatz, et al. 2023. Respiratory aerosols and droplets in the transmission of infectious diseases. Rev. Mod. Phys. 95 (4):045001(49. doi: 10.1103/RevModPhys.95.045001.
  • Pouwels, S. D., J. K. Burgess, E. Verschuuren, and D. J. Slebos. 2021. The cellular composition of the lung lining fluid gradually changes from bronchus to alveolus. Respir. Res. 22 (1):285. doi: 10.1186/s12931-021-01882-x.
  • Reynolds, S. J., D. W. Black, S. S. Borin, G. Breuer, L. F. Burmeister, L. J. Fuortes, T. F. Smith, M. A. Stein, P. Subramanian, P. S. Thorne, and P. Whitten. 2001. Indoor environmental quality in six commercial office buildings in the midwest United States. Appl. Occup. Environ. Hyg. 16 (11):1065–77. doi: 10.1080/104732201753214170.
  • Risbeck, M. J., M. Z. Bazant, Z. Jiang, Y. M. Lee, K. H. Drees, and J. D. Douglas. 2021. Modeling and multiobjective optimization of indoor airborne disease transmission risk and associated energy consumption for building HVAC systems. Energy Build. 253:111497. doi: 10.1016/j.enbuild.2021.111497.
  • Roy, C. J., D. S. Reed, and J. A. Hutt. 2010. Aerobiology and inhalation exposure to biological select agents and toxins. Vet. Pathol. 47 (5):779–89. doi: 10.1177/0300985810378650.
  • Rutala, W. A., M. F. Gergen, B. M. Tande, and D. J. Weber. 2013. Rapid hospital room decontamination using ultraviolet (UV) light with a nanostructured UV-reflective wall coating. Infect. Control Hosp. Epidemiol. 34 (5):527–9. doi: 10.1086/67021.
  • Saidel, L. J., A. R. Goldfarb, and S. Waldman. 1952. The absorption spectra of amino acids in the region two hundred to two hundred and thirty millimicrons. J. Biol. Chem. 197 (1):285–91. doi: 10.1016/S0021-9258(18)55677-5.
  • Sagripanti, J. L., and C. D. Lytle. 2011. Sensitivity to ultraviolet radiation of Lassa, vaccinia, and Ebola viruses dried on surfaces. Arch. Virol. 156 (3):489–94. doi: 10.1007/s00705-010-0847-1.
  • Schuit, M., S. Gardner, S. Wood, K. Bower, G. Williams, D. Freeburger, and P. Dabisch. 2020. The influence of simulated sunlight on the inactivation of influenza virus in aerosols. J. Infect. Dis. 221 (3):372–8. doi: 10.1093/infdis/jiz582.
  • Shen, Y., J. M. Courtney, P. Anfinrud, and A. Bax. 2022. Hybrid measurement of respiratory aerosol reveals a dominant coarse fraction resulting from speech that remains airborne for minutes. Proc. Natl. Acad. Sci. USA 119 (26):e2203086119. doi: 10.1073/pnas.2203086119.
  • Sliney, D. H., and B. E. Stuck. 2021. A need to revise human exposure limits for ultraviolet UV-C radiation. Photochem. Photobiol. 97 (3):485–92. doi: 10.1111/php.13402.
  • Srivastava, S., X. Zhao, Ati. Manay, and Q. Chen. 2021. Effective ventilation and air disinfection system for reducing coronavirus disease 2019 (COVID-19) infection risk in office buildings. Sustain. Cities Soc. 75:103408.doi: 10.1016/j.scs.2021.103408.
  • Stadnytskyi, V., P. Anfinrud, and A. Bax. 2021. Breathing, speaking, coughing or sneezing: What drives transmission of SARS-CoV-2? J. Intern. Med. 290 (5):1010–27. doi: 10.1111/joim.13326.
  • Stimson, M. M., and M. A. Reuter. 1943. Ultraviolet absorption spectra of nitrogenous heterocycles. VII. Effect of hydroxy substitutions on the ultraviolet absorption of the series: Hypoxanthine, xanthine and uric acid. J. Am. Chem. Soc. 65 (2):153–5. doi: 10.1021/ja01242a006.
  • Sun, W., and Q. Fu. 2000. Finite-difference time-domain solution of light scattering by dielectric particles with large complex refractive indices. Appl. Opt. 39 (30):5569–78. doi: 10.1364/AO.39.005569.
  • Thomas, R. J. 2013. Particle size and pathogenicity in the respiratory tract. Virulence. 4 (8):847–58. doi: 10.4161/viru.27172.
  • Thornton, D. J., K. Rousseau, and M. A. McGuckin. 2008. Structure and function of the polymeric mucins in airways mucus. Annu. Rev. Physiol. 70 (1):459–86. doi: 10.1146/annurev.physiol.70.113006.100702.
  • Tinglev, A. D., S. Ullah, G. Ljungkvist, E. Viklund, A. C. Olin, and O. Beck. 2016. Characterization of exhaled breath particles collected by an electret filter technique. J. Breath Res. 10 (2):026001. doi: 10.1088/1752-7155/10/2/026001.
  • Vejerano, E. P., and L. C. Marr. 2017. Physico-chemical characteristics of evaporating respiratory fluid droplets. J. R Soc. Interface 15 (139):0939. doi: 10.1098/rsif.2017.0939.
  • Veldhuizen, R., K. Nag, S. Orgeig, and F. Possmayer. 1998. The role of lipids in pulmonary surfactant. Biochim. Biophys. Acta. 1408 (2–3):90–108. doi: 10.1016/S0925-4439(98)00061-1.
  • Verheyen, C. A., and L. Bourouiba. 2022. Associations between indoor relative humidity and global COVID-19 outcomes. J. R Soc. Interface 19 (196):20210865. doi: 10.1098/rsif.2021.0865.
  • Weis, C. P., A. J. Intrepido, A. K. Miller, P. G. Cowin, M. A. Durno, J. S. Gebhardt, and R. Bull. 2002. Secondary aerosolization of viable Bacillus anthracis spores in a contaminated US Senate office. JAMA 288 (22):2853–8. doi: 10.1001/jama.288.22.2853.
  • Welch, D., N. J. Kleiman, P. C. Arden, K. L. Kuryla, M. Buonanno, B. Ponnaiya, X. Wu, and D. J. Brenner. 2023. No evidence of induced skin cancer or other skin abnormalities after long-term (66 week) chronic exposure to 222-nm far-UVC radiation. Photochem. Photobiol. 99 (1):168–75. doi: 10.1111/php.13656.
  • Wells, W. F., M. W. Wells, and T. S. Wilder. 1942. The environmental control of epidemic contagion. I. An epidemiologic study of radiant disinfection of air in day schools. Am J Hyg. 35 (1942):97–121.
  • Wetlaufer, D. B. 1963. Ultraviolet spectra of proteins and amino acids. Adv. Protein Chem. 17:303–90. doi: 10.1016/S0065-3233(08)60056-X.
  • Won, Y., D. Rim, R. Mistrick, and W. Bahnfleth. 2023. CFD modeling of room airflow effects on inactivation of aerosol SARS-CoV-2 by an upper-room ultraviolet germicidal irradiation (UVGI) system. Sci. Technol. Built Environ. 29 (7):719–29. doi: 10.1080/23744731.2023.2247947.
  • Wood, K., A. Wood, C. Peñaloza, and E. Eadie. 2022. Turn up the lights, leave them on and shine them all around—numerical simulations point the way to more efficient use of far-UVC lights for the inactivation of airborne coronavirus. Photochem. Photobiol. 98 (2):471–83. doi: 10.1111/php.13523.
  • Wyllie, A. L., J. Fournier, A. Casanovas-Massana, M. Campbell, M. Tokuyama, P. Vijayakumar, J. L. Warren, B. Geng, M. C. Muenker, A. J. Moore, et al. 2020. Saliva or nasopharyngeal swab specimens for detection of SARS-CoV-2. N Engl. J. Med. 383 (13):1283–6. doi: 10.1056/NEJMc2016359.
  • Xu, P., N. Fisher, and S. L. Miller. 2013. Using computational fluid dynamics modeling to evaluate the design of hospital ultraviolet germicidal irradiation systems for inactivating airborne mycobacteria. Photochem. Photobiol. 89 (4):792–8. doi: 10.1111/php.12062.
  • Yang, W., S. Elankumaran, and L. C. Marr. 2012. Relationship between humidity and influenza A viability in droplets and implications for influenza’s seasonality. PLoS One 7 (10):e46789. doi: 10.1371/journal.pone.0046789.
  • Yousefi, S., D. Simon, D. Stojkov, A. Karsonova, A. Karaulov, and H. U. Simon. 2020. In vivo evidence for extracellular DNA trap formation. Cell Death Dis. 11 (4):300. doi: 10.1038/s41419-020-2497-x.
  • Yurkin, M. A., and A. G. Hoekstra. 2011. The discrete-dipole-approximation code ADDA: Capabilities and known limitations. J. Quant. Spectrosc. Radiat. Transf. 112 (13):2234–47. doi: 10.1016/j.jqsrt.2011.01.031.
  • Zhang, H., and A. C. K. Lai. 2022. Evaluation of single-pass disinfection performance of far-UVC light on airborne microorganisms in duct flows. Environ. Sci. Technol. 56 (24):17849–57. doi: 10.1021/acs.est.2c04861.
  • Zhu, S., J. Srebric, S. N. Rudnick, R. L. Vincent, and E. A. Nardell. 2013. Numerical investigation of upper‐room UVGI disinfection efficacy in an environmental chamber with a ceiling fan. Photochem. Photobiol. 89 (4):782–91. doi: 10.1111/php.12039.
  • Zhu, S., S. Jenkins, K. Addo, M. Heidarinejad, S. A. Romo, A. Layne, J. Ehizibolo, D. Dalgo, N. W. Mattise, F. Hong, et al. 2020. Ventilation and laboratory confirmed acute respiratory infection (ARI) rates in college residence halls in College Park, Maryland. Environ. Int. 137:105537. doi: 10.1016/j.envint.2020.105537.
  • Zhu, S., T. Lin, L. Wang, E. A. Nardell, R. L. Vincent, and J. Srebric. 2022. Ceiling impact on air disinfection performance of Upper-Room Germicidal Ultraviolet (UR-GUV). Build. Environ. 224:109530. doi: 10.1016/j.buildenv.2022.109530.