Notch1 is a marker for in situ resting osteocytes in a 3-dimensional gel culture model

References

• Krishnan V, Dhurjati R, Vogler EA, Mastro AM. Osteogenesis in vitro: from pre-osteoblasts to osteocytes: a contribution from the osteobiology research group, the Pennsylvania State University. Vitro Cell Dev Biol Anim. 2010 Jan;46(1):28–35. doi:10.1007/s11626-009-9238-x.
   PubMed Web of Science ®Google Scholar
• Galli C, Passeri G, Macaluso GM. Osteocytes and WNT: the mechanical control of bone formation. J Dent Res. 2010 Apr;89(4):331–343. doi:10.1177/0022034510363963.
   PubMed Web of Science ®Google Scholar
• Schaffler MB, Cheung WY, Majeska R, Kennedy O. Osteocytes: master orchestrators of bone. Calcif Tissue Int. 2014 Jan;94(1):5–24. doi:10.1007/s00223-013-9790-y.
   PubMed Web of Science ®Google Scholar
• Bonewald LF. The role of the osteocyte in bone and nonbone disease. Endocrinol Metab Clin North Am. 2017 Mar;46(1):1–18. doi:10.1016/j.ecl.2016.09.003.
   PubMed Web of Science ®Google Scholar
• Hinton PV, Rackard SM, Kennedy OD. In Vivo osteocyte mechanotransduction: recent developments and future directions. Curr Osteoporos Rep. 2018 Dec;16(6):746–753. doi:10.1007/s11914-018-0485-1.
   PubMed Web of Science ®Google Scholar
• Robling AG, Bonewald LF. The Osteocyte: new Insights. Annu Rev Physiol Feb. 2020;10(82):485–506. doi:10.1146/annurev-physiol-021119-034332.
   Web of Science ®Google Scholar
• Guo Y, Tang CY, Man XF, Tang HN, Tang J, Wang F, Zhou CL, Tan SW, Feng YZ, Zhou HD. Insulin receptor substrate-1 time-dependently regulates bone formation by controlling collagen Iα2 expression via miR-342. Faseb J. 2016 Dec;30(12):4214–4226. doi:10.1096/fj.201600445RR.
   PubMed Web of Science ®Google Scholar
• El Deeb Zakhary I, Wenger K, Elsalanty M, Cray J, Sharawy M, Messer R. Characterization of primary osteocyte-like cells from rat mandibles. Oral Surg Oral Med Oral Pathol Oral Radiol. 2017 Jan;123(1):37–43. doi:10.1016/j.oooo.2016.08.020.
   PubMed Web of Science ®Google Scholar
• Li L, Tang P, Li S, Qin X, Yang H, Wu C, Liu Y. Notch signaling pathway networks in cancer metastasis: a new target for cancer therapy. Med Oncol. Sep 16 2017;34(10):180. doi10.1007/s12032-017-1039-6
   PubMed Web of Science ®Google Scholar
• Zanotti S, Canalis E. Parathyroid hormone inhibits Notch signaling in osteoblasts and osteocytes. Bone. 2017Oct;103: 159–167.10.1016/j.bone.2017.06.027.
   PubMed Web of Science ®Google Scholar
• Hilton MJ, Tu X, Wu X, Bai S, Zhao H, Kobayashi T, Kronenberg HM, Teitelbaum SL, Ross FP, Kopan R. Notch signaling maintains bone marrow mesenchymal progenitors by suppressing osteoblast differentiation. Nat Med. 2008 Mar;14(3):306–314. doi:10.1038/nm1716.
   PubMed Web of Science ®Google Scholar
• Zanotti S, Canalis E. Notch signaling and the skeleton. Endocr Rev. 2016 Jun;37(3):223–253. doi:10.1210/er.2016-1002.
   PubMed Web of Science ®Google Scholar
• Canalis E. Wnt signalling in osteoporosis: mechanisms and novel therapeutic approaches. Nat Rev Endocrinol. 2013 Oct;9(10):575–583. doi:10.1038/nrendo.2013.154.
   PubMed Web of Science ®Google Scholar
• Canalis E, Adams DJ, Boskey A, Parker K, Kranz L, Zanotti S. Notch signaling in osteocytes differentially regulates cancellous and cortical bone remodeling. J Biol Chem. Aug 30 2013;288(35):25614–25625. doi:10.1074/jbc.M113.470492.
   PubMed Web of Science ®Google Scholar
• Shao J, Zhou Y, Lin J, Nguyen TD, Huang R, Gu Y, Friis T, Crawford R, Xiao Y. Notch expressed by osteocytes plays a critical role in mineralisation. J Mol Med (Berl). 2018 Apr;96(3–4):333–347. doi:10.1007/s00109-018-1625-x.
   PubMed Web of Science ®Google Scholar
• Canalis E, Parker K, Feng JQ, Zanotti S. Osteoblast lineage-specific effects of notch activation in the skeleton. Endocrinology. 2013 Feb;154(2):623–634. doi:10.1210/en.2012-1732.
   PubMed Web of Science ®Google Scholar
• Shah KM, Stern MM, Stern AR, Pathak JL, Bravenboer N, Bakker AD. Osteocyte isolation and culture methods. Bonekey Rep. 2016;5:838. doi:10.1038/bonekey.2016.65.
   PubMedGoogle Scholar
• Guo Y, Tang CY, Man XF, Tang HN, Tang J, Zhou CL, Tan SW, Wang M, Feng YZ, Zhou HD. Insulin-like growth factor-1 promotes osteogenic differentiation and collagen I alpha 2 synthesis via induction of mRNA-binding protein LARP6 expression. Dev Growth Differ. 2017 Feb;59(2):94–103. doi:10.1111/dgd.12342.
   PubMed Web of Science ®Google Scholar
• Wu W, Fan H, Jiang Y, Liao L, Li L, Zhao J, Zhang H, Shrestha C, Xie Z. Regulation of 25-hydroxyvitamin D-1-hydroxylase and 24-hydroxylase in keratinocytes by PTH and FGF23. Exp Dermatol. 2018 Nov;27(11):1201–1209. doi:10.1111/exd.13760.
   PubMed Web of Science ®Google Scholar
• Elsdale T, Bard J. Collagen substrata for studies on cell behavior. J Cell Biol. 1972 Sep;54(3):626–637. doi:10.1083/jcb.54.3.626.
   PubMed Web of Science ®Google Scholar
• Matthews BG, Naot D, Callon KE, Musson DS, Locklin R, Hulley PA, Grey A, Cornish J. Enhanced osteoblastogenesis in three-dimensional collagen gels. Bonekey Rep. 2014;3:560.
   PubMedGoogle Scholar
• Hesse E, Hefferan TE, Tarara JE, Haasper C, Meller R, Krettek C, Lu L, Yaszemski MJ. Collagen type I hydrogel allows migration, proliferation, and osteogenic differentiation of rat bone marrow stromal cells. J Biomed Mater Res A. 2010 Aug;94(2):442–449. doi:10.1002/jbm.a.32696.
   PubMed Web of Science ®Google Scholar
• Plotkin LI, Bellido T. Osteocytic signalling pathways as therapeutic targets for bone fragility. Nat Rev Endocrinol. 2016 Oct;12(10):593–605. doi:10.1038/nrendo.2016.71.
   PubMed Web of Science ®Google Scholar
• Bitar M, Brown RA, Salih V, Kidane AG, Knowles JC, Nazhat SN. Effect of cell density on osteoblastic differentiation and matrix degradation of biomimetic dense collagen scaffolds. Biomacromolecules. 2008 Jan;9(1):129–135. doi:10.1021/bm701112w.
   PubMed Web of Science ®Google Scholar
• Casser-Bette M, Murray AB, Closs EI, Erfle V, Schmidt J. Bone formation by osteoblast-like cells in a three-dimensional cell culture. Calcif Tissue Int. 1990 Jan;46(1):46–56. doi:10.1007/bf02555824.
   PubMed Web of Science ®Google Scholar
• Bernhardt A, Weiser E, Wolf S, Vater C, Gelinsky M. Primary human osteocyte networks in pure and modified collagen gels. Tissue Eng Part A. 2019 Oct;25(19–20):1347–1355. doi:10.1089/ten.TEA.2018.0338.
   PubMed Web of Science ®Google Scholar
• Mc Garrigle MJ, Mullen CA, Haugh MG, Voisin MC, McNamara LM. Osteocyte differentiation and the formation of an interconnected cellular network in vitro. Eur Cell Mater. May 24 2016;31:323–340. 10.22203/ecm.v031a21
   PubMed Web of Science ®Google Scholar
• Holmbeck K, Bianco P, Pidoux I, Inoue S, Billinghurst RC, Wu W, Chrysovergis K, Yamada S, Birkedal-Hansen H, Poole AR. The metalloproteinase MT1-MMP is required for normal development and maintenance of osteocyte processes in bone. J Cell Sci. Jan 1 2005;118(Pt 1):147–156. doi10.1242/jcs.01581
   PubMed Web of Science ®Google Scholar
• Modinger Y, Loffler B, Huber-Lang M, Ignatius A. Complement involvement in bone homeostasis and bone disorders. Semin Immunol. 2018Jun;37: 53–65.10.1016/j.smim.2018.01.001.
   PubMed Web of Science ®Google Scholar
• Hill CR, Yuasa M, Schoenecker J, Goudy SL. Jagged1 is essential for osteoblast development during maxillary ossification. Bone. 2014 May;62:10–21. doi:10.1016/j.bone.2014.01.019.
   PubMed Web of Science ®Google Scholar
• Tu X, Chen J, Lim J. Physiological notch signaling maintains bone homeostasis via RBPjk and Hey upstream of NFATc1. PLoS Genet. 2012;8(3):e1002577. doi:10.1371/journal.pgen.1002577.
   PubMed Web of Science ®Google Scholar
• Zanotti S, Canalis E. Notch signaling in skeletal health and disease. Eur J Endocrinol. 2013 Jun;168(6):R95–103. doi:10.1530/EJE-13-0115.
   PubMed Web of Science ®Google Scholar
• Tamamura Y, Sakamoto K, Katsube KI, Yamaguchi A. Notch signaling is involved in Fgf23 upregulation in osteocytes. Biochem Biophys Res Commun. Oct 15 2019;518(2):233–238. doi:10.1016/j.bbrc.2019.08.038.
   PubMed Web of Science ®Google Scholar
• Dishowitz MI, Zhu F, Sundararaghavan HG, Ifkovits JL, Burdick JA, Hankenson KD. Jagged1 immobilization to an osteoconductive polymer activates the Notch signaling pathway and induces osteogenesis. J Biomed Mater Res A. 2014 May;102(5):1558–1567. doi:10.1002/jbm.a.34825.
   PubMed Web of Science ®Google Scholar
• Zanotti S, Canalis E. The Dmp1-SOST transgene interacts with and downregulates the Dmp1-Cre transgene and the Rosa(Notch) allele. J Cell Biochem. 2016 May;117(5):1222–1232. doi:10.1002/jcb.25405.
   PubMed Web of Science ®Google Scholar
• Sudo H, Kodama HA, Amagai Y, Yamamoto S, Kasai S. In vitro differentiation and calcification in a new clonal osteogenic cell line derived from newborn mouse calvaria. J Cell Biol. 1983 Jan;96(1):191–198. doi:10.1083/jcb.96.1.191.
   PubMed Web of Science ®Google Scholar
• Quarles LD, Yohay DA, Lever LW, Caton R, Wenstrup RJ. Distinct proliferative and differentiated stages of murine MC3T3-E1 cells in culture: an in vitro model of osteoblast development. J Bone Miner Res. 1992 Jun;7(6):683–692. doi:10.1002/jbmr.5650070613.
   PubMed Web of Science ®Google Scholar