3,790
Views
29
CrossRef citations to date
0
Altmetric
ORIGINAL ARTICLES

Development of a duplex real-time TaqMan PCR assay with an internal control for the detection of Mycoplasma gallisepticum and Mycoplasma synoviae in clinical samples from commercial and backyard poultry

, , , , , , , , & show all
Pages 99-109 | Received 17 Aug 2009, Published online: 08 Apr 2010

References

  • Avakian , A.P. and Kleven , S.H. 1990 . The humoral immune response of chickens to Mycoplasma gallisepticum and potential causes of false positive reactions in avian mycoplasma serology . Zentralblatt fur Bakteriologie Suppl , 20 : 500 – 512 .
  • Benčina , D. , Tadina , T. and Dorrer , D. 1988 . Natural infection of ducks with Mycoplasma synoviae and Mycoplasma gallisepticum and mycoplasma egg transmission . Avian Pathology , 17 : 441 – 449 .
  • Bradbury , J.M. 2005 . Poultry mycoplasmas: sophisticated pathogens in simple guise . British Poultry Science , 46 : 125 – 136 .
  • Bradbury , J.M. , Abdul-Wahab , O.S.M. , Yavari , C.A. , Dupiellet , J.P. and Bove , J.M. 1993 . Mycoplasma imitans sp. nov. is related to Mycoplasma gallisepticum and found in birds . International Journal of Systematic Bacteriology , 43 : 721 – 728 .
  • Buntz , B. , Bradbury , J.M. , Vuillaume , A. and Rousselot-Paillet , D. 1986 . Isolation of Mycoplasma gallisepticum from geese . Avian Pathology , 15 : 615 – 617 .
  • Callison , S.A. , Riblet , S.M. , Sun , S. , Ikuta , N. , Hilt , D. Leiting , V. 2006 . Development and validation of a real-time TaqMan polymerase chain reaction assay for the detection of Mycoplasma gallisepticum in naturally infected birds . Avian Diseases , 50 : 537 – 544 .
  • Carli , K.T. and Eyigor , A. 2003 . Real-time polymerase chain reaction for detection of Mycoplasma gallisepticum in chicken trachea . Avian Diseases , 47 : 712 – 717 .
  • Cookson , K.C. and Shivaprasad , H.L. 1994 . Mycoplasma gallisepticum infection in chukar partridges, pheasants, and peafowl . Avian Diseases , 38 : 914 – 921 .
  • Dupiellet J.P. 1984 Mycoplasmes et acholeplasmes des palmipedes a foie gras: isolement caractérisation, étude du rôle dans la pathologie Rappport de D.E.A. de Pathologie Université de Bordeaux II, Villenave d' Ornon, France
  • Edwards , M.C. and Gibbs , R.A. 1994 . Multiplex PCR: advantages, development, and applications . Genome Research , 3 : 65 – S75 .
  • Garcia , M. , Ikuta , N. , Levisohn , S. and Kleven , S.H. 2005 . Evaluation and comparison of various PCR methods for detection of Mycoplasma gallisepticum infection in chickens . Avian Diseases , 49 : 125 – 132 .
  • Garcia , M. , Jackwood , M.W. , Levisohn , S. and Kleven , S.H. 1995 . Detection of Mycoplasma gallisepticum, Mycoplasma synoviae, and Mycoplasma iowae by multi-species polymerase chain reaction and restriction fragment length polymorphism . Avian Diseases , 39 : 606 – 616 .
  • Ge , Z. , White , D. , Whary , M. and Fox , J. 2001 . Fluorogenic PCR-based quantitative detection of a murine pathogen, Helicobacter hepaticus . Journal of Clinical Microbiology , 35 : 2598 – 2602 .
  • Georgiades , G. , Iordanidis , P. and Koumbati , M. 2001 . Cases of swollen head syndrome in broiler chickens in Greece . Avian Diseases , 45 : 745 – 750 .
  • Grodio , J.L. , Dhondt , K.V. , O'Connell , P.H. and Schat , K.A. 2008 . Detection and quantification of Mycoplasma gallisepticum genome load in conjunctival samples of experimentally infected house finches (Carpodacus mexicanus) using real-time polymerase chain reaction . Avian Pathology , 37 : 385 – 391 .
  • Hammond , P.P. , Ramírez , A.S. , Morrow , C.J. and Bradbury , J.M. 2009 . Development and evaluation of an improved diagnostic PCR for Mycoplasma synoviae using primers located in the haemagglutinin encoding gene vlhA and its value for strain typing . Veterinary Microbiology , 136 ( 1–2 ) : 61 – 68 .
  • Hess , M. , Neubauer , C. and Hackl , R. 2007 . Interlaboratory comparison of ability to detect nucleic acid of Mycoplasma gallisepticum and Mycoplasma synoviae by polymerase chain reaction . Avian Pathology , 36 : 127 – 133 .
  • Hong , Y. , García , M. , Leiting , L. , Benčina , D. , Dufour-Zavala , L. , Zavala , G. and Kleven , S.H. 2004 . Specific detection and typing of Mycoplasma synoviae strains in poultry with PCR and DNA sequence analysis targeting the hemagglutinin encoding gene vlhA . Avian Diseases , 48 : 606 – 616 .
  • Jarquin , R. , Schultz , J. , Hanning , I. and Ricke , S. 2009 . Development of a real-time polymerase chain reaction assay for the simultaneous detection of Mycoplasma gallisepticum and Mycoplasma synoviae under industry conditions . Avian Diseases , 53 : 73 – 77 .
  • Kempf , I. , Gesbert , F. and Guittet , M. 1997 . Experimental infection of chickens with an atypical Mycoplasma gallisepticum strain: comparison of diagnostic methods . Research in Veterinary Science , 63 : 211 – 213 .
  • Khan , M.I. and Kleven , S.H. 1993 . Detection of Mycoplasma gallisepticum infection in field samples using a species-specific DNA probe . Avian Diseases , 37 : 880 – 883 .
  • Kleven , S.H. 1975 . Antibody response to avian mycoplasmas . American Journal of Veterinary Research , 36 : 563 – 565 .
  • Kleven , S.H. 1997 . “ Mycoplasma synoviae infection ” . In Diseases of Poultry , 10th edn , Edited by: Calnek , B.W. , Barnes , H.J. , Beard , C.W. , McDougald , L.R. and Saif , Y.M. 220 – 227 . Ames : Iowa State Press .
  • Kleven , S.H. 1998 . “ Mycoplasmosis ” . In A Laboratory Manual for the Isolation and Identification of Avian Pathogen , 4th edn , Edited by: Swayne , D.E. , John , R. , Jackwood , M.W. , Pearson , J.E. and Reed , W.M. 100 – 105 . Kennett Square , PA : University of Pennsylvania, New Bolton Center .
  • Kleven , S.H. , Jordan , F.T.W. and Bradbury , J.M. 1996 . “ Avian mycoplasmosis (Mycoplasma gallisepticum) ” . In Manual of Standards for Diagnostic Tests and Vaccines , Edited by: Reichard , R. 512 – 521 . Paris : Office International des Epizooties .
  • Lauerman , L.H. 1998 . “ Mycoplasma PCR assays ” . In Nucleic Acid Amplification Assays for Diagnosis of Animal Diseases , Edited by: Lauerman , L.H. 41 – 52 . Davis , CA : American Association of Veterinary Laboratory Diagnosticians .
  • Levisohn , S. and Kleven , S.H. 2000 . “ Avian mycoplasmosis (Mycoplasma gallisepticum) ” . In Diseases of Poultry: World Trade and Public Health Implications , Edited by: Beard , C.W. and McNulty , S. Vol. 19 , 425 – 442 . Paris : Office International des Epizooties .
  • Ley D.H. 2003 Mycoplasma gallisepticum infection In Y.M. Saif H.J. Barnes J.R. Glisson A.M. Fadly L.R. McDougald D.E. Swayne Diseases of Poultry , 11th edn 722 744 Ames , IA Iowa State Press
  • Ley , D.H. and Yoder , H.W. Jr . 1997 . “ Mycoplasma gallisepticum infection ” . In Diseases of Poultry , 10th edn , Edited by: Calnek , B.W. , Barnes , H.J. , Beard , C.W. , McDougald , L.R. and Saif , Y.M. 194 – 207 . Ames : Iowa State Press .
  • Mardassi , B.B.A. , Mohamed , R.B. , Gueriri , I. , Boughattas , S. and Milk , B. 2005 . Duplex PCR to differentiate Mycoplasma synoviae and Mycoplasma gallisepticum on the basis of conserved species-specific sequences of their hemagglutinin genes . Journal of Clinical Microbiology , 43 : 948 – 958 .
  • Marois , C. , Dufour-Gesbert , F. and Kempf , I. 2002 . Polymerase chain reaction for detection of Mycoplasma gallisepticum in environmental samples . Avian Pathology , 31 : 163 – 168 .
  • Mekkes , D.R. and Feberwee , A. 2005 . Real-time polymerase chain reaction for the qualitative and quantitative detection of Mycoplasma gallisepticum . Avian Pathology , 34 : 348 – 354 .
  • Nascimento , E.R. , Yamamoto , R. , Herrick , K.R. and Tait , R.T. 1991 . Polymerase chain reaction for detection of Mycoplasma gallisepticum . Avian Diseases , 35 : 62 – 69 .
  • OIE 2008 OIE Quality Standard and Guidelines for Veterinary Laboratories: Infectious Diseases Paris Office international des Epizooties
  • Pang , Y. , Wang , H. , Girshick , T. , Xie , Z. and Khan , M.J. 2002 . Development and application of a multiplex polymerase chain reaction for avian respiratory agents . Avian Diseases , 46 : 691 – 699 .
  • Papazisi , L. , Gorton , T.S. , Kutish , G. , Markham , P.F. , Browning , G.F. Nguyen , D.K. 2003 . The complete genome sequence of the avian pathogen Mycoplasma gallisepticum strain Rlow . Microbiology , 149 : 2307 – 2316 .
  • Ramírez , A.S. , Naylor , C.J. , Hammond , P.P. and Bradbury , J.M. 2006 . Development and evaluation of a diagnostic PCR for Mycoplasma synoviae using primers located in the intergenic spacer region and the 23S rRNA gene . Veterinary Microbiology , 118 : 76 – 82 .
  • Raviv , Z. , Ferguson-Noel , N. , Laibinis , V. , Wooten , R. and Kleven , S.H. 2007 . Role of Mycoplasma synoviae in commercial layer Escherichia coli peritonitis syndrome . Avian Diseases , 51 : 685 – 690 .
  • Raviv , Z. and Kleven , S.H. 2009 . The development of diagnostic real-time TaqMan PCRs for the four pathogenic avian mycoplasmas . Avian Diseases , 53 : 103 – 107 .
  • Rosendal , S. and Black , F.T. 1972 . Direct and indirect immunofluorescence of unfixed and fixed mycoplasma colonies . Acta Pathologica et Microbiologica Scandinavica, B , 80 : 615 – 622 .
  • Salisch , H. , Hinz , K.H. , Graack , H.D. and Ryll , M. 1998 . A comparison of a commercial PCR-based test to culture methods for detection of Mycoplasma gallisepticum and Mycoplasma synoviae in concurrently infected chickens . Avian Pathology , 27 : 142 – 147 .
  • Vasconcelos , A.T. , Ferreira , H.B. , Bizarro , C.V. , Bonatto , S.L. , Carvalho , M.O. Pinto , P.M. 2005 . Swine and poultry pathogens: The complete genome sequences of two strains of Mycoplasma hyopneumoniae and a strain of Mycoplasma synoviae . Journal of Bacteriology , 187 : 5568 – 5577 .
  • Wang , H. , Fadl , A.A. and Khan , M.I. 1997 . Multiplex PCR for avian pathogenic mycoplasma . Molecular and Cellular Probes , 11 : 211 – 216 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.