Heterologous expression and activity of Ptr ToxB from virulent and avirulent isolates of Pyrenophora tritici-repentis

References

• Ali , S. and Francl , L. J. 2003 . Population race structure of Pyrenophora tritici-repentis prevalent on wheat and noncereal grasses in the Great Plains . Plant Dis. , 87 : 418 – 422 .
   PubMed Web of Science ®Google Scholar
• Bailey , K. L. 1996 . Disease under conservation tillage system . Can. J. Plant Sci. , 76 : 635 – 639 .
   Web of Science ®Google Scholar
• Ballance , G. M. , Lamari , L. , Kowatsch , R. and Bernier , C. C. 1996 . Cloning, expression and occurrence of the gene encoding the Ptr necrosis toxin from Pyrenophora tritici-repentis . Mol. Plant Pathol. , [serial online]. [accessed 2 January 2007].
   Google Scholar
• Bjellqvist , B. , Hughes , G. J. , Pasquali , C. , Paquet , N. , Ravier , F. , Sanchez , J. C. , Frutiger , S. and Hochstrasser , D. F. 1993 . The focusing positions of polypeptides in immobilized pH gradients can be predicted from their amino acid sequences . Electrophoresis , 14 : 1023 – 1031 .
   PubMed Web of Science ®Google Scholar
• Blom , N. , Gammeltoft , S. and Brunak , S. 1999 . Sequence- and structure-based prediction of eukaryotic protein phosphorylation sites . J. Mol. Biol , 294 : 1351 – 1362 .
   PubMed Web of Science ®Google Scholar
• Bockus , W. W. and Claasen , M. M. 1992 . Effects of crop rotation and residue management practices on severity of tan spot of wheat . Plant Dis. , 76 : 633 – 636 .
   Web of Science ®Google Scholar
• Bradford , M. M. 1976 . A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding . Anal. Biochem. , 72 : 248 – 254 .
   PubMed Web of Science ®Google Scholar
• Ciuffetti , L. M. , Tuori , R. P. and Gaventa , J. M. 1997 . A single gene encodes a selective toxin causal to the development of tan spot of wheat . Plant Cell , 9 : 135 – 144 .
   PubMed Web of Science ®Google Scholar
• Ciuffetti , L. M. , Francl , L. J. , Ballance , G. M. , Bockus , W. W. , Lamari , L. , Meinhardt , S. W. and Rassmussen , J. B. 1998 . Standardization of toxin nomenclature in the Pyrenophora tritici-repentis/wheat interaction . Can. J. Plant Pathol. , 20 : 421 – 424 .
   Web of Science ®Google Scholar
• Dhingra , O. D. and Sinclair , J. B. 1986 . “ Culture media and their formulations ” . In Basic plant pathology methods , 285 – 315 . Boca Raton, Fla : CRC Press .
   Google Scholar
• Effertz , R. J. , Meinhardt , S. W. , Anderson , J. A. , Jordahl , J. G. and Francl , L. J. 2002 . Identification of a chlorosis-inducing toxin from Pyrenophora tritici-repentis and the chromosomal location of an insensitivity locus in wheat . Phytopathology , 92 : 527 – 533 .
   PubMed Web of Science ®Google Scholar
• Gamba , F. M. and Lamari , L. 1998 . Mendelian inheritance of resistance to tan spot (Pyrenophora tritici-repentis) in selected genotypes of durum wheat (Triticum turgidum) . Can. J. Plant Pathol. , 20 : 408 – 414 .
   Web of Science ®Google Scholar
• Gamba , F. M. , Lamari , L. and Brûlé-Babel , A. L. 1998 . Inheritance of race-specific necrotic and chlorotic reactions induced by Pyrenophora tritici-repentis in hexaploid wheats . Can. J. Plant Pathol. , 20 : 401 – 407 .
   Web of Science ®Google Scholar
• Güssow , D. and Clackson , T. 1989 . Direct clone characterization from plaques and colonies by the polymerase chain reaction . Nucleic Acids Res. , 17 : 4000
   PubMed Web of Science ®Google Scholar
• Hagborg , W. A. F. 1970 . A device for injecting solutions and suspensions into thin leaves of plants . Can. J. Bot , 48 : 1135 – 1136 .
   Google Scholar
• Hosford , R. M. Jr. Tan spot—developing knowledge 1902–1981, virulent races and wheat differentials, methodology, rating systems, other leaf diseases, literature . Proceedings of Tan Spot of Wheat and Related Diseases Workshop . July 1981 , Fargo, N.D.. Edited by: Hosford , R. M. Jr. pp. 1 – 24 . Fargo, N.D. : North Dakota Agricultural Experimental Station, North Dakota State University .
   Google Scholar
• Jones , D. T. 1999 . Protein secondary structure prediction based on position-specific scoring matrices . J. Mol. Biol. , 292 : 195 – 202 .
   PubMed Web of Science ®Google Scholar
• Lamari , L. and Bernier , C. C. 1989 . Evaluation of wheat lines and cultivars to tan spot (Pyrenophora tritici-repentis) based on lesion type . Can. J. Plant Pathol. , 11 : 49 – 56 .
   Web of Science ®Google Scholar
• Lamari , L. and Bernier , C. C. 1991 . Genetics of tan necrosis and extensive chlorosis in tan spot of wheat caused by Pyrenophora tritici-repentis . Phyto pathology , 81 : 1092 – 1095 .
   Web of Science ®Google Scholar
• Lamari , L. , Sayoud , R. , Boulif , M. and Bernier , C. C. 1995 . Identification of a new race in Pyrenophora tritici-repentis: implications for the current pathotype classification system . Can. J. Plant Pathol. , 17 : 312 – 318 .
   Web of Science ®Google Scholar
• Lamari , L. , Gilbert , J. and Tekauz , A. 1998 . Race differentiation in Pyrenophora tritici-repentis and survey of physiologic variation in western Canada . Can. J. Plant Pathol. , 20 : 396 – 400 .
   Web of Science ®Google Scholar
• Lamari , L. , Strelkov , S. E. , Yahyaoui , A. , Orabi , J. and Smith , R. B. 2003 . The identification of two new races of Pyrenophora tritici-repentis from the host centre of diversity confirms a one-to-one relationship in tan spot of wheat . Phytopathology , 93 : 391 – 396 .
   PubMed Web of Science ®Google Scholar
• Lambert , C. , Leonard , N. , De Bolle , X. and Depiereux , E. 2002 . ESyPred3D: prediction of proteins 3D structures . Bioinformatics , 18 : 1250 – 1256 .
   PubMed Web of Science ®Google Scholar
• Landy , A. 1989 . Dynamic, structural, and regulatory aspects of lambda site-specific recombination . Annu. Rev. Biochem. , 58 : 913 – 941 .
   PubMed Web of Science ®Google Scholar
• Lichtenthaler , H. K. 1987 . Chlorophyll and carotenoids: pigments of photosynthetic membranes . Methods Enzymol , 148 : 350 – 382 .
   Web of Science ®Google Scholar
• Lund , O. , Nielsen , M. , Lundegaard , C. and Worning , P. CPHmodels 2.0: X3M a computer program to extract 3D models . Abstracts of the Fifth Community Wide Experiment on the Critical Assessment of Techniques for Protein Structure Prediction (CASP5) Conference . December 1–5 2002 . pp. 102 – 104 . Asilomar, Calif [Abstr.]. [accessed 2 January 2007].
   Google Scholar
• Manning , V. A. , Pandelova , I. and Ciuffetti , L. M. 2002 . A race for a novel host-selective toxin . Pythopathology , 92 : S51 [Abstr.]
   PubMedGoogle Scholar
• Manning , V. A. , Andrie , R. M. , Trippe , A. F. and Ciuffetti , L. M. 2004 . Ptr ToxA requires multiple motifs for complete activity . Mol. Plant-Microbe Interact. , 17 : 491 – 501 .
   PubMedGoogle Scholar
• Martinez , J. P. , Ottum , S. A. , Ali , S. , Francl , L. J. and Ciuffetti , L. M. 2001 . Characterization of the ToxB gene from Pyrenophora tritici-repentis . Mol. Plant-Microbe Interact. , 14 : 675 – 677 .
   PubMed Web of Science ®Google Scholar
• Martinez , J. P. , Oesch , N. W. and Ciuffetti , L. M. 2004 . Characterization of the multiple-copy host-selective toxin gene, ToxB, in pathogenic and nonpathogenic isolates of Pyrenophora tritici-repentis . Mol. Plant-Microbe Interact. , 17 : 467 – 474 .
   PubMed Web of Science ®Google Scholar
• Meinhardt , S. W. , Cheng , W. , Kwon , C. Y. , Donohue , C. M. and Rasmussen , J. B. 2002 . Role of the arginyl-glycyl-aspartic motif in the action of Ptr ToxA produced by Pyrenophora tritici-repentis . Plant Physiol. , 130 : 1545 – 1551 .
   PubMedGoogle Scholar
• Meinhardt , S. , Ali , S. , Ling , H. and Francl , L. A new race of Pyrenophora tritici-repentis that produces a putative host-selective toxin . Proceedings of the Fourth International Wheat Tan Spot and Spot Blotch Workshop . July 21–24 2002 , Bemidji, Minn. Edited by: Rasmussen , J. B. , Friesen , T. L. and Ali , S. pp. 117 – 119 . Fargo, N.D. : North Dakota Agricultural Experimental Station, North Dakota State University .
   Google Scholar
• Parks , T. D. , Howard , E. D. , Wolpert , T. J. , Arp , D. J. and Dougherty , W. G. 1995 . Expression and purification of a recombinant tobacco etch virus NIa proteinase: biochemical analyses of the full-length and a naturally occuring truncated proteinase form . Virology , 210 : 194 – 201 .
   PubMed Web of Science ®Google Scholar
• Rogers , S. O. and Bendich , A. J. 1994 . “ Extraction of total cellular DNA from plants, algae and fungi ” . In Plant molecular biology manual. , 2nd ed. , Edited by: Gelv , S. B. and Schilperoort , R. A. Vol. D1 , 1 – 8 . Dordrecht, , the Netherlands : Kluwer Academic Press .
   Google Scholar
• Rozen , S. and Skaletsky , H. J. 2000 . “ Primer3 on the WWW for general users and for biologist programmers ” . In Bioinformatics methods and protocols: methods in molecular biology , Edited by: Krawetz , S. and Misener , S. 365 – 386 . Totowa, N.J : Humana Press .
   Google Scholar
• Sambrook , J. , Fritsch , E. F. and Maniatis , T. 1989 . Molecular cloning: a laboratory manual. , 2nd ed. , Cold Spring Harbor, N.Y : Cold Spring Harbor Laboratory Press .
   Google Scholar
• Schagger , H. and von Jagow , G. 1987 . Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa . Anal. Biochem. , 166 : 368 – 379 .
   PubMed Web of Science ®Google Scholar
• Strelkov , S. E. 2002 . Occurrence and expression of ToxB and comparative virulence of chlorosis inducing races of Pyrenophora tritici-repentis , Winnipeg, Man : University of Manitoba . Ph.D. thesis
   Google Scholar
• Strelkov , S. E. and Lamari , L. 2003 . Host-parasite interactions in tan spot [Pyrenophora tritici-repentis] of wheat . Can. J. Plant Pathol. , 25 : 339 – 349 .
   Web of Science ®Google Scholar
• Strelkov , S. E. , Lamari , L. and Ballance , G. M. 1998 . Induced chlorophyll degradation by a chlorosis toxin from Pyrenophora tritici-repentis . Can. J. Plant Pathol. , 20 : 428 – 435 .
   Web of Science ®Google Scholar
• Strelkov , S. E. , Lamari , L. and Ballance , G. M. 1999 . Characterization of a host-specific protein toxin (Ptr ToxB) from Pyrenophora tritici-repentis . Mol. Plant-Microbe Interact. , 12 : 728 – 732 .
   Web of Science ®Google Scholar
• Strelkov , S. E. , Lamari , L. , Sayoud , R. and Smith , R. B. 2002 . Comparative virulence of chlorosis-inducing races of Pyrenophora tritici-repentis . Can. J. Plant Pathol. , 24 : 29 – 35 .
   Web of Science ®Google Scholar
• Strelkov , S. E. , Kowatsch , R. F. , Ballance , G. M. and Lamari , L. 2005 . Characterization of the ToxB gene from North African and Canadian isolates of Pyrenophora tritici-repentis . Physiol. Mol. Plant Pathol. , 67 : 164 – 170 .
   Google Scholar
• Witham , F. H. , Blaydes , D. F. and Devlin , R. M. 1971 . “ Experiment 15 ” . In Experiments in plant physiology , 55 – 58 . New York : Van Nostrand Reinhold Co .
   Google Scholar