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Canadian Journal of Plant Pathology Volume 40, 2018 - Issue 3
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Genomics/Génomique

A duplex PCR method for identification of cultures of Fusarium graminearum from infected wheat grain without DNA extraction

Krista ZuzakThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
,
Kher ZahrThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
,
Yalong YangThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
,
Alian SarkesThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
,
David FeindelThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
,
Greg DanielsCrop Diversification Centre South, Alberta Agriculture and Forestry, Brooks, Alberta, T1R 1E6, CanadaView further author information
,
Michael W. HardingCrop Diversification Centre South, Alberta Agriculture and Forestry, Brooks, Alberta, T1R 1E6, CanadaCorrespondence[email protected] [email protected]
View further author information
&
Jie FengThe Alberta Plant Health Lab, Crop Diversification Centre North, Alberta Agriculture and Forestry, Edmonton, T5Y 6H3, CanadaView further author information
 show all
Pages 417-422 | Accepted 24 May 2018, Published online: 12 Jun 2018

References

  • Alberta Agriculture and Forestry. 2017. Fusarium head blight - frequently asked questions. http://www1.agric.gov.ab.ca/$department/deptdocs.nsf/all/faq12513.
  • Demeke T, Clear RM, Patrick SK, Gaba D. 2005. Species-specific PCR-based assays for the detection of Fusarium species and a comparison with the whole seed agar plate method and trichothecene analysis. Inter J Food Microbiol. 103:271–284.
  • Doohan FM, Parry DW, Jenkinson P, Nicholson P. 1998. The use of species-specific PCR-based assays to analyze Fusarium ear blight of wheat. Plant Pathol. 47:197–205.
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  • Parry DW, Nicholson P. 1996. Development of a PCR assay to detect Fusarium poae in wheat. Plant Pathol. 45:383–391.
  • Pasquali M, Giraud F, Brochot C, Hoffmann L, Bohn T. 2009. First report of the nivalenol chemotype of Fusarium graminearum causing head blight of wheat in the Grand Duchy of Luxembourg. Plant Dis. 93:1217.
  • Przemieniecki SW, Kurowski TP, Korzekwa K. 2014. Chemotypes and geographic distribution of the Fusarium graminearum species complex. Environ Biotechnol. 10:45–59.
  • Sadhasivam S, Britzi M, Zakin V, Kostyukovsky M, Trostanetsky A, Quinn E, Sionov E. 2017. Rapid detection and identification of mycotoxigenic fungi and mycotoxins in stored wheat grain. Toxins. 9:302.
  • Tekauz A, McCallum B, Gilbert J. 2000. Fusarium head blight of barley in western Canada. Can J Plant Pathol. 22:9–16.
  • White TJ, Bruns T, Lee S, Taylor J. 1990. Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innes MA, Gelfand DH, Sninsky JJ, White TJ, eds. PCR protocols: a guide to methods and applications. San Diego: Academic Press; p. 315–322.

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