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Canadian Journal of Plant Pathology Volume 8, 1986 - Issue 1
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PATHOLOGY

A Rapid Procedure for the Purification of Potato Spindle Tuber Viroid — Use of Highperformance Gel Permeation Chromatography

R.P. Singh Research Station, Agriculture Canada, P.O. Box 20280, Fredericton, New Brunswick, E3B 4Z7
,
D. Levesque Research Station, Agriculture Canada, P.O. Box 20280, Fredericton, New Brunswick, E3B 4Z7
&
R.R. King Research Station, Agriculture Canada, P.O. Box 20280, Fredericton, New Brunswick, E3B 4Z7
Pages 54-58 | Accepted 15 Jan 1986, Published online: 29 Dec 2009

References

  • Colpan , M. and Riesner , D. 1984 . High-performance liquid chromatography of high-molecular-weight nucleic acids on the macroporous ion exchanger, Nucleogen . J. Chromatogr. , 296 : 339 – 353 .
  • Colpan , M. , Schumacher , J. , Bruggemann , W. , Sanger , H. L. and Riesner , D. 1983 . Large scale purification of viroid RNA using CS2CS04 gradient centrifugation and high performance liquid chromatography . Anal. Biochem. , 131 : 257 – 265 .
  • Diener , T. O. 1971 . Potato spindle tuber “virus”. IV. A replicating, low molecular weight RNA . Virology , 45 : 411 – 428 .
  • Diener , T. O. , Hadidi , A. and Owens , R. A. 1977 . “ Methods for studying viroids ” . In Methods in virology , Edited by: Maramorosch , K. and Koprowski , H. Vol. 6 , 185 – 217 . New York and London : Academic Press .
  • Graeve , L. and Kruppa , J. 1983 . Purification of lowmolecular weight RNAs by high-performance gel permeation chromatography . J. Chromatogr. , 268 : 506 – 510 .
  • Gross , H. J. , Domedy , H. , Jank , P. , Lossow , C. , Raba , M. , Alberty , H. and Sanger , H. L. 1978 . Nucleotide sequence and secondary structure of potato spindle tuber viroid . Nature , 273 : 203 – 208 .
  • Niblett , CL. , Dickson , E. , Horst , R. K. and Romaine , C. P. 1980 . Additional hosts and an efficient purification procedure for four viroids . Phytopathology , 70 : 610 – 615 .
  • Pfannenstiel , M. A. , Slack , S. A. and Lane , L. C. 1980 . Detection of potato spindle tuber viroid in field-grown potatoes by improved electrophoretic assay . Phytopathology , 70 : 1015 – 1018 .
  • Sanger , H. L. , Klotz , G. , Riesner , D. , Gross , H. J. and Kleinschmidt , A. K. 1976 . Viroids are single-stranded covalently closed circular RNA molecules existing as highly basepaired rod-like structures . Proc. Natl. Acad. Sci. USA , 73 : 3852 – 3856 .
  • Semancik , J. S. and Szychowski , J. 1983 . Enhanced detection of viroid-RNA after selective divalent cation fractionation . Anal. Biochem. , 135 : 275 – 279 .
  • Singh , R. P. 1973 . Experimental host-range of the potato spindle tuber “virus . Am. Potato J. , 50 : 111 – 123 .
  • Singh , R. P. 1982 . Evaluation of procedures for the detection of potato spindle tuber viroid by polyacrylamide gel electrophoresis . Can. Plant Dis. Surv. , 62 : 41 – 44 .
  • Singh , R. P. 1984 . Solanum * berthaultii, a sensitive host for indexing potato spindle tuber viroid from dormant tubers . Potato Res. , 27 : 163 – 172 .
  • Singh , R. P. and Clark , M. C. 1971 . Infectious lowmolecular weight ribonucleic acid from tomato Biochem, Biophys . Res. Commun. , 44 : 1077 – 1082 .
  • Singh , R. P. , Michniewicz , J. J. and Narang , S. A. 1976 . Separation of potato spindle tuber viroid ribonucleic acid from Scopolia sinensis into three infectious forms and the purification and oligonucleotide patterns of fraction II RNA . Can. J. Biochem. , 54 : 600 – 608 .

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