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- We earlier showed 7h,i that a mixture of cis- and trans-3b prepared from reaction of 1.0 g of 4 with Me2NCl can be separated in a single pass on an MPLC column to give 200 mg of cis-3b and 500 mg of trans-3b. (15 × 1000 mm column of Merck 230–400 mesh Si02; CHCl3/CH3OH, 97/3)