Bioactive components from Moringa oleifera seeds: production, functionalities and applications – a critical review

References

• Padayachee B, Baijnath H. An overview of the medicinal importance of Moringaceae. J Med Plants Res. 2012;6(48):5831–5839.
   Google Scholar
• Leone A, Spada A, Battezzati A, et al. Cultivation, genetic, ethnopharmacology, phytochemistry and pharmacology of Moringa oleifera leaves: an overview. Int J Mol Sci. 2015;16(6):12791–12835.
   PubMed Web of Science ®Google Scholar
• Fahey JW. Moringa oleifera: a review of the medical evidence for its nutritional, therapeutic, and prophylactic properties. Part 1. Trees Life J. 2005;1(5):1–15.
   Google Scholar
• Palada MC. Moringa (Moringa oleifera Lam.): a versatile tree crop with horticultural potential in the subtropical United States. HortSci. 1996;31(5):794–797.
   Web of Science ®Google Scholar
• Popoola JO, Obembe OO. Local knowledge, use pattern and geographical distribution of Moringa oleifera Lam. (Moringaceae) in Nigeria. J Ethnopharmacol. 2013;150(2):682–691.
   PubMed Web of Science ®Google Scholar
• Rockwood J, Anderson B, Casamatta D. Potential uses of Moringa oleifera and an examination of antibiotic efficacy conferred by M. oleifera seed and leaf extracts using crude extraction techniques available to underserved indigenous populations. Int J Phytother Res. 2013;3(2):61–71.
   Google Scholar
• Vats S, Gupta T. Evaluation of bioactive compounds and antioxidant potential of hydroethanolic extract of Moringa oleifera Lam. from Rajasthan, India. Physiol Mol Biol Plants. 2017;23(1):239–248.
   PubMed Web of Science ®Google Scholar
• Kasolo JN, Bimenya GS, Ojok L, et al. Phytochemicals and uses of Moringa oleifera leaves in Ugandan rural communities. J Med Plants Res. 2010;4(9):753–757.
   Google Scholar
• Nair S, Varalakshmi K. Anticancer, cytotoxic potential of Moringa oleifera extracts on HeLa cell line. J Nat Pharm. 2011;2(3):138–142.
   Google Scholar
• Sutalangka C, Wattanathorn J, Muchimapura S, et al. Moringa oleifera mitigates memory impairment and neurodegeneration in animal model of age-related dementia. Oxid Med Cell Longev. 2013;2013:695936.
   PubMed Web of Science ®Google Scholar
• Saa RW, Fombang EN, Ndjantou EB, et al. Njintang NYJFs, nutrition. Treatments and uses of Moringa oleifera seeds in human nutrition: a review. Food Sci Nutr. 2019;7(6):1911–1919.
   PubMed Web of Science ®Google Scholar
• Saini RK, Sivanesan I, Keum Y-S. Phytochemicals of Moringa oleifera: a review of their nutritional, therapeutic and industrial significance. 3 Biotech. 2016;6(2):203.
   PubMedGoogle Scholar
• Aderinola TA, Monisola Alashi A, Fagbemi aN, et al. Moringa oleifera flour protein fractions as food ingredients with antioxidant properties. SDRP-JFST. 2019;4(4):720–728.
   Google Scholar
• Aderinola TA, Alashi AM, Nwachukwu ID, et al. Antihypertensive and antioxidant properties of Moringa oleifera seed enzymatic protein hydrolysate and ultrafiltration fractions. Curr Top Nutraceutical Res. 2019;17(4):437–444.
   Web of Science ®Google Scholar
• Aderinola TA, Alashi AM, Nwachukwu ID, et al. In vitro digestibility, structural and functional properties of Moringa oleifera seed proteins. Food Hydrocolloids. 2020;101:105574.
   Web of Science ®Google Scholar
• Aderinola TA, Fagbemi TN, Enujiugha VN, et al. In vitro antihypertensive and antioxidative properties of alcalase-derived Moringa oleifera seed globulin hydrolysate and its membrane fractions. J Food Process Preserv. 2019;43(2):e13862.
   Web of Science ®Google Scholar
• Aderinola TA, Fagbemi TN, Enujiugha VN, et al. In vitro antihypertensive and antioxidative properties of trypsin-derived Moringa oleifera seed globulin hydrolyzate and its membrane fractions. Food Sci Nutr. 2019;7(1):132–138.
   PubMed Web of Science ®Google Scholar
• Aderinola TA, Fagbemi TN, Enujiugha VN, et al. Amino acid composition and antioxidant properties of Moringa oleifera seed protein isolate and enzymatic hydrolysates. Heliyon. 2018;4(10):e00877.
   PubMed Web of Science ®Google Scholar
• Garza NGG, Koyoc JAC, Castillo JAT, et al. Biofunctional properties of bioactive peptide fractions from protein isolates of moringa seed (Moringa oleifera). J Food Sci Technol. 2017;54(13):4268–4276.
   PubMed Web of Science ®Google Scholar
• Liang L, Wang C, Li S, et al. Nutritional compositions of Indian Moringa oleifera seed and antioxidant activity of its polypeptides. Food Sci Nutr. 2019;7(5):1754–1760.
   PubMed Web of Science ®Google Scholar
• Liang L-L, Cai S-Y, Gao M, et al. Purification of antioxidant peptides of Moringa oleifera seeds and their protective effects on H2O2 oxidative damaged Chang liver cells. J Funct Foods. 2020;64:103698.
   Web of Science ®Google Scholar
• Leone A, Spada A, Battezzati A, et al. Moringa oleifera seeds and oil: characteristics and uses for human health. IJMS. 2016;17(12):2141.
   Google Scholar
• Vergara-Jimenez M, Almatrafi MM, Fernandez ML. Bioactive components in Moringa oleifera leaves protect against chronic disease. Antioxidants. 2017;6(4):91.
   Web of Science ®Google Scholar
• Alain Mune Mune M, Nyobe EC, Bakwo Bassogog C, et al. A comparison on the nutritional quality of proteins from Moringa oleifera leaves and seeds. Cogent Food Agric. 2016;2(1):1–8.
   Google Scholar
• Mutiara T, Titi E, Estiasih W. Effect lactagogue Moringa leaves (Moringa oleifera Lam) powder in rats white female wistar. J Basic Appl Sci Res. 2013;3(4):430–434.
   Google Scholar
• Richter N, Siddhuraju P, Becker KJA. Evaluation of nutritional quality of moringa (Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia (Oreochromis niloticus L.). Aquaculture. 2003;217(1–4):599–611.
   Web of Science ®Google Scholar
• Khor KZ, Lim V, Moses EJ, et al. Abdul Samad N. The in vitro and in vivo anticancer properties of Moringa oleifera. Evid Based Complement Alternat Med. 2018;2018:1071243.
   PubMed Web of Science ®Google Scholar
• Falowo AB, Mukumbo FE, Idamokoro EM, et al. Multi-functional application of Moringa oleifera Lam. in nutrition and animal food products: a review. Food Res Int. 2018;106:317–334.
   PubMed Web of Science ®Google Scholar
• Busani M, Patrick JM, Arnold H, et al. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. Afr J Biotechnol. 2011;10(60):12925–12933.
   Google Scholar
• Ma Z, Ahmad J, Zhang H, et al. Evaluation of phytochemical and medicinal properties of Moringa (Moringa oleifera) as a potential functional food. S Afr J Bot. 2020;129:40–46.
   Web of Science ®Google Scholar
• Yamaguchi NU, Cusioli LF, Quesada HB, et al. A review of Moringa oleifera seeds in water treatment: trends and future challenges. Process Saf Environ Prot. 2020;147:405–420.
   Web of Science ®Google Scholar
• Anwar F, Rashid U. Physico-chemical characteristics of Moringa oleifera seeds and seed oil from a wild provenance of Pakistan. Pak J Bot. 2007;39(5):1443–1453.
   Web of Science ®Google Scholar
• Adamczyk B, Simon J, Kitunen V, et al. Tannins and their complex interaction with different organic nitrogen compounds and enzymes: old paradigms versus recent advances. ChemistryOpen. 2017;6(5):610–614.
   PubMed Web of Science ®Google Scholar
• Biesalski, HK, Grimm P. Pocket atlas of nutrition (No. Ed. 3 enlarged & updated). Georg Thieme Verlag; 2004.
   Google Scholar
• Singh RG, Negi PS, Radha C. Phenolic composition, antioxidant and antimicrobial activities of free and bound phenolic extracts of Moringa oleifera seed flour. J Funct Foods. 2013;5(4):1883–1891.
   Web of Science ®Google Scholar
• Singh BN, Singh B, Singh R, et al. Oxidative DNA damage protective activity, antioxidant and anti-quorum sensing potentials of Moringa oleifera. Food Chem Toxicol. 2009;47(6):1109–1116.
   PubMed Web of Science ®Google Scholar
• Padla EP, Solis LT, Levida RM, et al. Antimicrobial isothiocyanates from the seeds of Moringa oleifera Lam. Z Naturforsch C J Biosci. 2012;67(11–12):557–564.
   PubMed Web of Science ®Google Scholar
• Guevara AP, Vargas C, Sakurai H, et al. An antitumor promoter from Moringa oleifera Lam. Mutat Res/Genet Toxicol Environ Mutagen. 1999;440(2):181–188.
   PubMed Web of Science ®Google Scholar
• Oluduro OA, Aderiye BI, Connolly JD, et al. Characterization and antimicrobial Activity of 4-(β-D-glucopyranosyl-1→4-α-L-rhamnopyranosyloxy)-benzyl thiocarboxamide; a novel bioactive compound from Moringa oleifera seed extract. Folia Microbiol. 2010;55(5):422–426.
   PubMed Web of Science ®Google Scholar
• Aja P, Nwachukwu N, Ibiam U, et al. Chemical constituents of Moringa oleifera leaves and seeds from Abakaliki, Nigeria. Am J Phytomed Clin Ther. 2014;2(3):310–321.
   Google Scholar
• Rim Jeon S, Ha Lee K, Ha Shin D, et al. Synergistic antimicrobial efficacy of mesoporous ZnO loaded with 4-(α-L-rhamnosyloxy)-benzyl isothiocyanate isolated from the Moringa oleifera seed. J Gen Appl Microbiol. 2014;60(6):251–255.
   PubMed Web of Science ®Google Scholar
• Galuppo M, Nicola GR, Iori R, et al. Antibacterial activity of glucomoringin bioactivated with myrosinase against two important pathogens affecting the health of long-term patients in hospitals. Molecules. 2013;18(11):14340–14348.
   PubMed Web of Science ®Google Scholar
• Galuppo M, Giacoppo S, De Nicola GR, et al. Antiinflammatory activity of glucomoringin isothiocyanate in a mouse model of experimental autoimmune encephalomyelitis. Fitoterapia. 2014;95:160–174.
   PubMed Web of Science ®Google Scholar
• Giacoppo S, Galuppo M, De Nicola GR, et al. 4(α-l-rhamnosyloxy)-benzyl isothiocyanate, a bioactive phytochemical that attenuates secondary damage in an experimental model of spinal cord injury . Bioorg Med Chem. 2015;23(1):80–88.
   PubMed Web of Science ®Google Scholar
• Jaja-Chimedza A, Zhang L, Wolff K, et al. A dietary isothiocyanate-enriched moringa (Moringa oleifera) seed extract improves glucose tolerance in a high-fat-diet mouse model and modulates the gut microbiome. J Funct Foods. 2018;47:376–385.
   PubMed Web of Science ®Google Scholar
• Jaja-Chimedza A, Graf BL, Simmler C, et al. Biochemical characterization and anti-inflammatory properties of an isothiocyanate-enriched moringa (Moringa oleifera) seed extract. PLOS One. 2017;12(8):e0182658.
   PubMed Web of Science ®Google Scholar
• Amagloh FK, Benang A. Effectiveness of Moringa oleifera seed as coagulant for water purification. Afr J Agric Res. 2009;4(1):119–123.
   Google Scholar
• Amina M, Al Musayeib NM, Alarfaj NA, et al. Exploiting the potential of Moringa oleifera oil/polyvinyl chloride polymeric bionanocomposite film enriched with silver nanoparticles for antimicrobial activity. Int J Polym Sci. 2019;2019:1–11.
   Web of Science ®Google Scholar
• Ayerza R. Seed yield components, oil content, and fatty acid composition of two cultivars of moringa (Moringa oleifera Lam.) growing in the Arid Chaco of Argentina. Ind Crops Prod. 2011;33(2):389–394.
   Web of Science ®Google Scholar
• Elsayed EA, Sharaf-Eldin MA, Wadaan M. In vitro evaluation of cytotoxic activities of essential oil from Moringa oleifera seeds on HeLa, HepG2, MCF-7, CACO-2 and L929 cell lines. Asian Pac J Cancer Prev. 2015;16(11):4671–4675.
   PubMedGoogle Scholar
• Abiodun O, Adegbite J, Omolola A. Chemical and physicochemical properties of Moringa flours and oil. Global J Sci Front Res Biol Sci. 2012;12(5):1–7.
   Google Scholar
• Ijarotimi OS, Adeoti OA, Ariyo O. Comparative study on nutrient composition, phytochemical, and functional characteristics of raw, germinated, and fermented Moringa oleifera seed flour. Food Sci Nutr. 2013;1(6):452–463.
   PubMedGoogle Scholar
• Mbah B, Eme P, Ogbusu O. Effect of cooking methods (boiling and roasting) on nutrients and anti-nutrients content of Moringa oleifera seeds. Pak J Nutr. 2012;11(3):211–215.
   Google Scholar
• Moyo B, Masika PJ, Hugo A, et al. Nutritional characterization of Moringa (Moringa oleifera Lam.) leaves. African J Biotechnol. 2011;10(60):12925–12933.
   Google Scholar
• Lurling M, Beekman W. Anti-cyanobacterial activity of Moringa oleifera seeds. J Appl Phycol. 2010;22(4):503–510.
   PubMed Web of Science ®Google Scholar
• Hamza AA. Ameliorative effects of Moringa oleifera Lam seed extract on liver fibrosis in rats. Food Chem Toxicol. 2010;48(1):345–355.
   PubMed Web of Science ®Google Scholar
• Mahajan SG, Mehta AA. Inhibitory action of ethanolic extract of seeds of Moringa oleifera Lam. on systemic and local anaphylaxis. J Immunotoxicol. 2007;4(4):287–294.
   PubMed Web of Science ®Google Scholar
• Mahajan SG, Mali RG, Mehta AA. Protective effect of ethanolic extract of seeds of Moringa oleifera Lam. against inflammation associated with development of arthritis in rats. J Immunotoxicol. 2007;4(1):39–47.
   PubMed Web of Science ®Google Scholar
• Nieman DC, Butterworth DE, Nieman CN. Nutrition. Dubugye (MI): Wm. C. Brown; 1992. p. 237–312
   Google Scholar
• Compaoré WR, Nikièma PA, Bassolé HIN, et al. Chemical composition and antioxidative properties of seeds of Moringa oleifera and pulps of Parkia biglobosa and Adansonia digitata commonly used in food fortification in Burkina Faso. Current Res J Bio Sci. 2011;3(1):64–72.
   Google Scholar
• Faso B. Nutritional properties of enriched local complementary flours. Adv J Food Sci Technol. 2011;3(1):31–39.
   Google Scholar
• Bridgemohan P, Bridgemohan R, Mohamed M. Chemical composition of a high protein animal supplement from Moringa oleifera. African J Food Sci Technol. 2014;5(5):125–128.
   Google Scholar
• Mattila P, Mäkinen S, Eurola M, et al. Nutritional value of commercial protein-rich plant products. Plant Foods Hum Nutr. 2018;73(2):108–115.
   PubMed Web of Science ®Google Scholar
• Esther O, Oladipo A. Preliminary test of phytochemical screening of crude extracts of Moringa oleifera Seed. J Appl Chem. 2012;3(2):11–13.
   Google Scholar
• Sinha S. Phytochemical analysis and antibacterial potential of Moringa oleifera Lam. Int J Sci Innovations Discoveries. 2012;2(4):401–407.
   Google Scholar
• Idris MA, Jami MS, Hammed AM, et al. Moringa oleifera seed extract: a review on its environmental applications. Int J Appl Environ Sci. 2016;11(6):1469–1486.
   Google Scholar
• Mehta S, Rai PK, Rai NK, et al. Role of spectral studies in detection of antibacterial phytoelements and phytochemicals of Moringa oleifera. Food Biophys. 2011;6(4):497–502.
   Web of Science ®Google Scholar
• Abd Rani NZ, Husain K, Kumolosasi E. Moringa genus: a review of phytochemistry and pharmacology. Front Pharmacol. 2018;9:108.
   PubMedGoogle Scholar
• Acquah C, Agyei D, Obeng EM, et al. Aptamers: an emerging class of bioaffinity ligands in bioactive peptide applications. Crit Rev Food Sci Nutr. 2020;60(7):1195–1206.
   PubMed Web of Science ®Google Scholar
• Acquah C, Dzuvor CK, Tosh S, et al. Anti-diabetic effects of bioactive peptides: recent advances and clinical implications. Crit Rev Food Sci Nutr. 2020;1–14.
   PubMed Web of Science ®Google Scholar
• Agyei D, Jeevanandam J, Dzuvor CKO, et al. Novel ingredients from cereals. Innovative Process Technol Healthy Grains. 2020;143–175.
   Google Scholar
• Agyei D, Pan S, Acquah C, et al. Bioactivity profiling of peptides from food proteins. Soft chemistry and food fermentation. Cambridge (MA): Elsevier; 2017. p. 49–77.
   Google Scholar
• Gnasegaran GK, Agyei D, Pan S, et al. Process development for bioactive peptide production. Food Bioactives. Cham: Switzerland: Springer; 2017. p. 91–110.
   Google Scholar
• Dzuvor CKO, Taylor JT, Acquah C, et al. Bioprocessing of functional ingredients from flaxseed. Molecules. 2018;23(10):2444.
   Web of Science ®Google Scholar
• Fotouo-M H, Du Toit ES, Robbertse PJ. Germination and ultrastructural studies of seeds produced by a fast-growing, drought-resistant tree: implications for its domestication and seed storage. AoB Plants. 2015;7:1–12.
   PubMed Web of Science ®Google Scholar
• Anwar F, Ashraf M, Bhanger MI. Interprovenance variation in the composition of Moringa oleifera oilseeds from Pakistan. J Amer Oil Chem Soc. 2005;82(1):45–51.
   Web of Science ®Google Scholar
• Kumar Ganesan S, Singh R, Choudhury DR, et al. Genetic diversity and population structure study of drumstick (Moringa oleifera Lam.) using morphological and SSR markers. Ind Crops Prod. 2014;60:316–325.
   Web of Science ®Google Scholar
• Rommi K, Hakala TK, Holopainen U, et al. Effect of enzyme-aided cell wall disintegration on protein extractability from intact and dehulled rapeseed (Brassica rapa L. and Brassica napus L.) press cakes. J Agric Food Chem. 2014;62(32):7989–7997.
   PubMed Web of Science ®Google Scholar
• Hadnadjev M, Dapcevic-Hadnadjev T, Pojic M, et al. Progress in vegetable proteins isolation techniques: a review. Food Feed Res. 2017;44(1):11–21.
   Google Scholar
• Branlard G, Bancel E. Protein extraction from cereal seeds. Plant Proteomics. London (UK): Springer. 2007. p. 15–25.
   Google Scholar
• Agyei D, Danquah MK. Industrial-scale manufacturing of pharmaceutical-grade bioactive peptides. Biotechnol Adv. 2011;29(3):272–277.
   PubMed Web of Science ®Google Scholar
• Avramenko NA, Chang C, Low NH, et al. Encapsulation of flaxseed oil within native and modified lentil protein-based microcapsules. Food Res Int. 2016;81:17–24.
   Web of Science ®Google Scholar
• Karamać M, Kosińska-Cagnazzo A, Kulczyk A. Use of different proteases to obtain flaxseed protein hydrolysates with antioxidant activity. IJMS. 2016;17(7):1027.
   Google Scholar
• Kim S-K, Wijesekara I. Development and biological activities of marine-derived bioactive peptides: a review. J Funct Foods. 2010;2(1):1–9.
   Web of Science ®Google Scholar
• Qi W, He Z. Enzymatic hydrolysis of protein: mechanism and kinetic model. Front Chem China. 2006;1(3):308–314.
   Google Scholar
• Tavano OL. Protein hydrolysis using proteases: an important tool for food biotechnology. J Mol Catal B: Enzym. 2013;90:1–11.
   Web of Science ®Google Scholar
• Castro HC, Abreu PA, Geraldo RB, et al. Looking at the proteases from a simple perspective. J Mol Recognit. 2011;24(2):165–181.
   PubMed Web of Science ®Google Scholar
• Karovičová ZK-J. Kohajdova J. Fermentation of cereals for specific purpose. J Food Nutr Res. 2007;46(2):51–57.
   Web of Science ®Google Scholar
• Missaoui J, Saidane D, Mzoughi R, et al. Fermented Seeds (“Zgougou”) from Aleppo Pine as a novel source of potentially probiotic lactic acid bacteria. Microorganisms. 2019;7(12):709.
   Web of Science ®Google Scholar
• Schaffner DW, Beuchat LR. Fermentation of aqueous plant seed extracts by lactic acid bacteria. Appl Environ Microbiol. 1986;51(5):1072–1076.
   PubMed Web of Science ®Google Scholar
• Hotz C, Gibson RS. Traditional food-processing and preparation practices to enhance the bioavailability of micronutrients in plant-based diets. J Nutr. 2007;137(4):1097–1100.
   PubMed Web of Science ®Google Scholar
• Chaves‐López C, Serio A, Grande‐Tovar CD, et al. Traditional fermented foods and beverages from a microbiological and nutritional perspective: the Colombian heritage. Compr Rev Food Sci Food Saf. 2014;13(5):1031–1048.
   Web of Science ®Google Scholar
• Alka S, Neelam Y, Shruti S. Effect of fermentation on physicochemical properties and in vitro starch and protein digestibility of selected cereals. Int J Agric Food Sci. 2012;2(3):66–70.
   Google Scholar
• Pranoto Y, Anggrahini S, Efendi Z. Effect of natural and Lactobacillus plantarum fermentation on in-vitro protein and starch digestibilities of sorghum flour. Food Biosci. 2013;2:46–52.
   Google Scholar
• Jude‐Ojei B, Lola A, Ajayi I, et al. Functional and pasting properties of maize ‘Ogi’ supplemented with fermented moringa seeds. J Food Process Technol. 2017;8(674):10–4172.
   Google Scholar
• Kumar V, Sinha AK, Makkar HP, et al. Dietary roles of phytate and phytase in human nutrition: a review. Food Chem. 2010;120(4):945–959.
   Web of Science ®Google Scholar
• Ortiz D, Nkhata S, Buechler A, et al. Nutritional changes during biofortified maize fermentation (steeping) for ogi production. FASEB J. 2017;31(1_supplement):32.4–4.
   Google Scholar
• Cavaliere C, Capriotti AL, La Barbera G, et al. Liquid chromatographic strategies for separation of bioactive compounds in food matrices. Molecules. 2018;23(12):3091.
   Web of Science ®Google Scholar
• Gratz S, Gamble B, Stalcup A. Inclusion complexation: liquid chromatography. Oxford: Academic Press; 2000. p. 3079–3086.
   Google Scholar
• Vailaya A, Horváth C. Retention in reversed-phase chromatography: partition or adsorption? J Chromatogr A. 1998;829(1–2):1–27.
   PubMed Web of Science ®Google Scholar
• Di Palma S, Hennrich ML, Heck AJ, et al. Recent advances in peptide separation by multidimensional liquid chromatography for proteome analysis. J Proteomics. 2012;75(13):3791–3813.
   PubMed Web of Science ®Google Scholar
• Ghebremichael K, Gunaratna K, Dalhammar G. Single-step ion exchange purification of the coagulant protein from Moringa oleifera seed. Appl Microbiol Biotechnol. 2006;70(5):526–532.
   PubMed Web of Science ®Google Scholar
• McCalley DV. Understanding and manipulating the separation in hydrophilic interaction liquid chromatography. J Chromatogr A. 2017;1523:49–71.
   PubMed Web of Science ®Google Scholar
• Lemes AC, Sala L, Ores JC, et al. A review of the latest advances in encrypted bioactive peptides from protein-rich waste. IJMS. 2016;17(6):950.
   Google Scholar
• Acquah C, Chan YW, Pan S, et al. Structure-informed separation of bioactive peptides . J Food Biochem. 2019;43(1):e12765.
   PubMed Web of Science ®Google Scholar
• Nawaz H, Shi J, Mittal GS, et al. Extraction of polyphenols from grape seeds and concentration by ultrafiltration. Sep Purif Technol. 2006;48(2):176–181.
   Web of Science ®Google Scholar
• Castel V, Andrich O, Netto FM, et al. Comparison between isoelectric precipitation and ultrafiltration processes to obtain Amaranth mantegazzianus protein concentrates at pilot plant scale. J Food Eng. 2012;112(4):288–295.
   Web of Science ®Google Scholar
• Saucedo-Pompa S, Torres-Castillo J, Castro-López C, et al. Moringa plants: bioactive compounds and promising applications in food products. Food Res Int. 2018;111:438–450.
   PubMed Web of Science ®Google Scholar
• Anudeep S, Radha C. Carbohydrates of Moringa oleifera seeds. Int J Res Anal Rev. 2018;5(4):103–108.
   Google Scholar
• Anudeep S, Prasanna VK, Adya SM, et al. Characterization of soluble dietary fiber from Moringa oleifera seeds and its immunomodulatory effects. Int J Biol Macromol. 2016;91:656–662.
   PubMed Web of Science ®Google Scholar
• Hayes M, Tiwari BK. Bioactive carbohydrates and peptides in foods: an overview of sources, downstream processing steps and associated bioactivities. Int J Mol Sci. 2015;16(9):22485–22508.
   PubMed Web of Science ®Google Scholar
• Garcia-Mendoza MP, Paula JT, Paviani LC, et al. Extracts from mango peel by-product obtained by supercritical CO2 and pressurized solvent processes. LWT-Food Sci Technol. 2015;62(1):131–137.
   Web of Science ®Google Scholar
• Mustafa A, Turner C. Pressurized liquid extraction as a green approach in food and herbal plants extraction: a review. Anal Chim Acta. 2011;703(1):8–18.
   PubMed Web of Science ®Google Scholar
• Soquetta MB, Terra LM, Bastos CP. Green technologies for the extraction of bioactive compounds in fruits and vegetables. CyTA-J Food. 2018;16(1):400–412.
   Web of Science ®Google Scholar
• Ruiz-Matute AI, Ramos L, Martínez-Castro I, et al. Fractionation of honey carbohydrates using pressurized liquid extraction with activated charcoal. J Agric Food Chem. 2008;56(18):8309–8313.
   PubMed Web of Science ®Google Scholar
• Subhedar PB, Gogate PR. Enhancing the activity of cellulase enzyme using ultrasonic irradiations. J Mol Catal B: Enzym. 2014;101:108–114.
   Google Scholar
• Herrero M, Cifuentes A, Ibañez E. Sub-and supercritical fluid extraction of functional ingredients from different natural sources: plants, food-by-products, algae and microalgae: a review. Food Chem. 2006;98(1):136–148.
   Web of Science ®Google Scholar
• Herrero M, Mendiola JA, Cifuentes A, et al. Supercritical fluid extraction: recent advances and applications. J Chromatogr A. 2010;1217(16):2495–2511.
   PubMed Web of Science ®Google Scholar
• Koçak E, Pazır F. Effect of extraction methods on bioactive compounds of plant origin. Turkish JAF Scitech. 2018;6(6):663–675.
   Google Scholar
• Barbary O, Al-Sohaimy S, El-Saadani M, et al. Extraction, composition and physicochemical properties of flaxseed mucilage. J Adv Agric Res. 2009;14:605–620.
   Google Scholar
• Farrán A, Cai C, Sandoval M, et al. Green solvents in carbohydrate chemistry: from raw materials to fine chemicals. Chem Rev. 2015;115(14):6811–6853.
   PubMed Web of Science ®Google Scholar
• Ojiako E, Okeke C. Determination of antioxidant of Moringa oleifera seed oil and its use in the production of a body cream. Asian J Plant Sci Res. 2013;3(3):1–4.
   Google Scholar
• Aviara N, Musa W, Owolarafe O, et al. Effect of processing conditions on oil point pressure of Moringa oleifera seed. J Food Sci Technol. 2015;52(7):4499–4506.
   PubMed Web of Science ®Google Scholar
• Bhutada PR, Jadhav AJ, Pinjari DV, et al. Solvent assisted extraction of oil from Moringa oleifera Lam. seeds. Ind Crops Prod. 2016;82:74–80.
   Web of Science ®Google Scholar
• Belo YN, Al-Hamimi S, Chimuka L, et al. Ultrahigh-pressure supercritical fluid extraction and chromatography of Moringa oleifera and Moringa peregrina seed lipids. Anal Bioanal Chem. 2019;411(16):3685–3693.
   PubMed Web of Science ®Google Scholar
• Janaki S. Characterization of cold press Moringa oil. Int J Sci Res. 2015;4:386–389.
   Google Scholar
• Latif S, Anwar F, Hussain AI, et al. Aqueous enzymatic process for oil and protein extraction from Moringa oleifera seed. Eur J Lipid Sci Technol. 2011;113(8):1012–1018.
   Web of Science ®Google Scholar
• Bhargavi G, Rao PN, Renganathan S. Review on the extraction methods of crude oil from all generation biofuels in last few decades. IOP Conf Ser: Mater Sci Eng. 2018;330:012024.
   Google Scholar
• Galvão EL, Martínez J, de Oliveira HNM, et al. Supercritical extraction of linseed oil: economical viability and modeling extraction curves. Chem Eng Commun. 2013;200(2):205–221.
   Web of Science ®Google Scholar
• Rubilar M, Gutiérrez C, Verdugo M, et al. Flaxseed as a source of functional ingredients. J Soil Sci Plant Nutr. 2010;10(3):373–377.
   Web of Science ®Google Scholar
• Shim YY, Gui B, Wang Y, et al. Flaxseed (Linum usitatissimum L.) oil processing and selected products. Trends Food Sci Technol. 2015;43(2):162–177.
   Web of Science ®Google Scholar
• Yu S, Hongkun X, Chenghai L, et al. Comparison of microwave assisted extraction with hot reflux extraction in acquirement and degradation of anthocyanin from powdered blueberry. Int J Agric Biol Eng. 2016;9(6):186–199.
   Web of Science ®Google Scholar
• Zhong J, Wang Y, Yang R, et al. The application of ultrasound and microwave to increase oil extraction from Moringa oleifera seeds. Ind Crops Prod. 2018;120:1–10.
   Web of Science ®Google Scholar
• Rosas-Mendoza ME, Coria-Hernández J, Meléndez-Pérez R, et al. Characteristics of chia (Salvia hispanica L.) seed oil extracted by ultrasound assistance. J Mex Chem Soc. 2017;61(4):326–335.
   Web of Science ®Google Scholar
• Nde DB, Foncha AC. Optimization methods for the extraction of vegetable oils: a review. Processes. 2020;8(2):209.
   Web of Science ®Google Scholar
• Alegbeleye OO. How functional is Moringa Oleifera? A review of its nutritive, medicinal, and socioeconomic potential. Food Nutr Bull. 2018;39(1):149–170.
   PubMed Web of Science ®Google Scholar
• Dhakad AK, Ikram M, Sharma S, et al. Biological, nutritional, and therapeutic significance of Moringa oleifera Lam. Phytother Res. 2019;33(11):2870–2903.
   PubMed Web of Science ®Google Scholar
• Lin M, Zhang J, Chen X. Bioactive flavonoids in Moringa oleifera and their health-promoting properties. J Funct Foods. 2018;47:469–479.
   Web of Science ®Google Scholar
• Randriamboavonjy JI, Loirand G, Vaillant N, et al. Cardiac protective effects of Moringa oleifera seeds in spontaneous hypertensive rats. Am J Hypertens. 2016;29(7):873–881.
   PubMed Web of Science ®Google Scholar
• Paula PC, Sousa DO, Oliveira JT, et al. A protein isolate from Moringa oleifera leaves has hypoglycemic and antioxidant effects in alloxan-induced diabetic mice. Molecules. 2017;22(2):271.
   Web of Science ®Google Scholar
• Abdulazeez A, Ajiboye O, Wudil A, et al. Partial purification and characterization of angiotensin converting enzyme inhibitory alkaloids and flavonoids from the leaves and seeds of Moringa oleifera. JABB. 2016;5(2):1–11.
   Google Scholar
• Al-Malki AL, El Rabey HA. The antidiabetic effect of low doses of Moringa oleifera Lam. seeds on streptozotocin induced diabetes and diabetic nephropathy in male rats. Biomed Res Int. 2015;2015:381040.
   PubMed Web of Science ®Google Scholar
• Fatoumata B, MamadouSaïdou B, Mohamet S, et al. Antidiabetic properties of Moringa oleifera: a review of the literature. J Diabetes Endocrinol. 2020;11(1):18–29.
   Google Scholar
• Vargas-Sánchez K, Garay-Jaramillo E, González-Reyes RE. Effects of Moringa oleifera on glycaemia and insulin levels: a review of animal and human studies. Nutrients. 2019;11(12):2907.
   Web of Science ®Google Scholar
• Gupta R, Kannan GM, Sharma M, et al. Therapeutic effects of Moringa oleifera on arsenic-induced toxicity in rats. Environ Toxicol Pharmacol. 2005;20(3):456–464.
   PubMed Web of Science ®Google Scholar
• Mahmood KT, Mugal T, Haq IU. Moringa oleifera: a natural gift – a review. J Pharm Sci Res. 2010;2(11):775.
   Google Scholar
• Yang R-Y, Chang L-C, Hsu J-C, et al. Nutritional and functional properties of moringa leaves from germplasm, to plant, to food, to health. Moringa leaves: strategies, standards and markets for a better impact on nutrition in Africa Moringanews. Paris: CDE, CTA, GFU; 2006.
   Google Scholar
• Lalas S, Tsaknis J. Characterization of Moringa oleifera seed oil variety Periyakulam 1. J Food Compos Anal. 2002;15(1):65–78.
   Web of Science ®Google Scholar
• Lalas S, Tsaknis J. Extraction and identification of natural antioxidant from the seeds of the Moringa oleifera tree variety of Malawi. J Amer Oil Chem Soc. 2002;79(7):677–683.
   Web of Science ®Google Scholar
• Yasa N, Masoumi F, Rouhani RS, et al. Chemical composition and antioxidant activity of the extract and essential oil of Rosa damascena from Iran, population of Guilan. J Fac Pharm. 2009;17(3):175–180.
   Google Scholar
• Minaiyan M, Asghari G, Taheri D, et al. Anti-inflammatory effect of Moringa oleifera Lam. seeds on acetic acid-induced acute colitis in rats. Avicenna J Phytomed. 2014;4(2):127–136.
   PubMedGoogle Scholar
• Adebayo IA, Arsad H, Samian MR. Antiproliferative effect on breast cancer (MCF7) of Moringa oleifera seed extracts. Afr J Tradit Complement Altern Med. 2017;14(2):282–287.
   PubMedGoogle Scholar
• Asaduzzaman A, Hasan I, Chakrabortty A, et al. Moringa oleifera seed lectin inhibits Ehrlich ascites carcinoma cell growth by inducing apoptosis through the regulation of Bak and NF-κB gene expression. Int J Biol Macromol. 2018;107(Pt B):1936–1944.
   PubMedGoogle Scholar
• Bharali R, Tabassum J, Azad MRH. Chemomodulatory effect of Moringa oleifera, Lam, on hepatic carcinogen metabolising enzymes, antioxidant parameters and skin papillomagenesis in mice. Asian Pac J Cancer Prev. 2003;4(2):131–140.
   PubMedGoogle Scholar
• Bharali DJ, Sahoo SK, Mozumdar S, et al. Cross-linked polyvinylpyrrolidone nanoparticles: a potential carrier for hydrophilic drugs. J Colloid Interface Sci. 2003;258(2):415–423.
   PubMed Web of Science ®Google Scholar
• Shebek K, Schantz AB, Sines I, et al. The flocculating cationic polypetide from Moringa oleifera seeds damages bacterial cell membranes by causing membrane fusion. Langmuir. 2015;31(15):4496–4502.
   PubMed Web of Science ®Google Scholar
• Pinto CE, Farias DF, Carvalho AF, et al. Food safety assessment of an antifungal protein from Moringa oleifera seeds in an agricultural biotechnology perspective. Food Chem Toxicol. 2015;83:1–9.
   PubMed Web of Science ®Google Scholar
• Bukar A, Uba A, Oyeyi T. Antimicrobial profile of Moringa oleifera Lam. extracts against some food-borne microorganisms. Bayero J Pure App Sci. 2010;3(1):43–48.
   Google Scholar
• Chuang P-H, Lee C-W, Chou J-Y, et al. Anti-fungal activity of crude extracts and essential oil of Moringa oleifera Lam. Bioresour Technol. 2007;98(1):232–236.
   PubMed Web of Science ®Google Scholar
• Ferreira R, Napoleão TH, Santos AF, et al. Coagulant and antibacterial activities of the water-soluble seed lectin from Moringa oleifera . Lett Appl Microbiol. 2011;53(2):186–192.
   PubMed Web of Science ®Google Scholar
• Emmanuel S, Olajide O, Abubakar S, et al. Phytochemical and antimicrobial studies of methanol, ethyl acetate, and aqueous extracts of Moringa oleifera seeds. Am J Ethnomed. 2014;1(5):346–354.
   Google Scholar
• Onsare J, Arora D. Antibiofilm potential of flavonoids extracted from Moringa oleifera seed coat against Staphylococcus aureus, Pseudomonas aeruginosa and Candida albicans. J Appl Microbiol. 2015;118(2):313–325.
   PubMed Web of Science ®Google Scholar
• Ali GH, EL‐Taweel GE, Ali MA. M. The cytotoxicity and antimicrobial efficiency of Moringa oleifera seeds extracts. Int J Environ Stud. 2004;61(6):699–708.
   Google Scholar
• Chollom S, Agada G, Gotep J, et al. Investigation of aqueous extract of Moringa oleifera lam seed for antiviral activity against newcastle disease virus in ovo. J Med Plants Res. 2012;6(22):3870–3875.
   Google Scholar
• Randriamboavonjy JI, Rio M, Pacaud P, et al. Moringa oleifera seeds attenuate vascular oxidative and nitrosative stresses in spontaneously hypertensive rats. Oxid Med Cell Longev. 2017;2017:4129459.
   PubMed Web of Science ®Google Scholar
• Faizi S, Siddiqui BS, Saleem R, et al. Hypotensive constituents from the pods of Moringa oleifera. Planta Med. 1998;64(3):225–228.
   PubMed Web of Science ®Google Scholar
• Kim Y, Wu AG, Jaja-Chimedza A, et al. Isothiocyanate-enriched moringa seed extract alleviates ulcerative colitis symptoms in mice. PLoS One. 2017;12(9):e0184709.
   PubMed Web of Science ®Google Scholar
• Batista AB, Oliveira JT, Gifoni JM, et al. New insights into the structure and mode of action of Mo-CBP3, an antifungal chitin-binding protein of Moringa oleifera seeds. PLOS One. 2014;9(10):e111427.
   PubMed Web of Science ®Google Scholar
• Prabhu K, Murugan K, Nareshkumar A, et al. Larvicidal and repellent potential of Moringa oleifera against malarial vector, Anopheles stephensi Liston (Insecta: Diptera: Culicidae). Asian Pac J Trop Biomed. 2011;1(2):124–129.
   PubMedGoogle Scholar
• Agra-Neto AC, Napoleão TH, Pontual EV, et al. Effect of Moringa oleifera lectins on survival and enzyme activities of Aedes aegypti larvae susceptible and resistant to organophosphate. Parasitol Res. 2014;113(1):175–184.
   PubMed Web of Science ®Google Scholar
• Salles HO, Braga ACL, do Nascimento MTdS, et al. Lectin, hemolysin and protease inhibitors in seed fractions with ovicidal activity against Haemonchus contortus. Rev Bras Parasitol Vet. 2014;23(2):136–143.
   PubMed Web of Science ®Google Scholar
• Sengupta ME, Keraita B, Olsen A, et al. Use of Moringa oleifera seed extracts to reduce helminth egg numbers and turbidity in irrigation water. Water Res. 2012;46(11):3646–3656.
   PubMed Web of Science ®Google Scholar
• Ashfaq M, Ashfaq U. Evaluation of mosquitocidal activity of water extract of Moringa oleifera seeds against Culex quinquefasciatus (Diptera: Culicidae) in Pakistan. Pak Entomol. 2012;34(1):21–26.
   Google Scholar
• Parwani L, Bhatnagar M, Bhatnagar A, et al. Evaluation of Moringa oleifera seed biopolymer-PVA composite hydrogel in wound healing dressing. Iran Polym J. 2016;25(11):919–931.
   Web of Science ®Google Scholar
• Bhatnagar M, Parwani L, Sharma V, et al. Hemostatic, antibacterial biopolymers from Acacia arabica (Lam.) Willd. and Moringa oleifera (Lam.) as potential wound dressing materials. Indian J Exp Biol. 2013;51(10):804–810.
   Google Scholar
• Bichi MH. A review of the applications of Moringa oleifera seeds extract in water treatment. Civil Environ Res. 2013;3(8):1–10.
   Google Scholar
• Gopalakrishnan L, Doriya K, Kumar DS. Moringa oleifera: a review on nutritive importance and its medicinal application. Food Sci Hum Wellness. 2016;5(2):49–56.
   Google Scholar
• Olagbemide PT, Philip C. Proximate analysis and chemical composition of raw and defatted Moringa oleifera kernel. Adv Life Science Technol. 2014;24:92–99.
   Google Scholar
• Nzikou J, Matos L, Moussounga J, et al. Characterization of Moringa oleifera seed oil variety Congo-Brazzaville. J Food Technol. 2009;7(3):59–65.
   Google Scholar
• Qu X, Alvarez PJ, Li Q. Applications of nanotechnology in water and wastewater treatment. Water Res. 2013;47(12):3931–3946.
   PubMed Web of Science ®Google Scholar
• Grady CL, Daigger GT Jr, Love NG, et al. Biological wastewater treatment. Boca Raton (FL): CRC Press; 2011.
   Google Scholar
• Gray N. How nature deals with waste. Biology of Wastewater Treatment. Covent Garden, London, UK: Imperial College Press; 2004. p. S1–14.
   Google Scholar
• Henze M, van Loosdrecht M, Ekama G, et al. Biological wastewater treatment IWA publishing. London: Cambridge University Press; 2008.
   Google Scholar
• Chen G. Electrochemical technologies in waste water treatment. Sep Purif Technol. 2004;38(1):11–41.
   Web of Science ®Google Scholar
• Berger M, Habs M, Jahn S, et al. Toxicological assessment of seeds from Moringa oleifera and Moringa stenopetala, two highly efficient primary coagulants for domestic water treatment of tropical raw waters. East Afr Med J. 1984;61(9):712–716.
   PubMed Web of Science ®Google Scholar
• Sotheeswaran S, Nand V, Maata M, et al. Moringa oleifera and other local seeds in water purification in developing countries. Res J Chem Environ. 2011;15(2):135–138.
   Google Scholar
• Ndabigengesere A, Narasiah KS. Use of Moringa oleifera seeds as a primary coagulant in wastewater treatment. Environ Technol. 1998;19(8):789–800.
   Web of Science ®Google Scholar
• Vieira AMS, Vieira MF, Silva GF, et al. Use of Moringa oleifera seed as a natural adsorbent for wastewater treatment. Water Air Soil Pollut. 2010;206(1–4):273–281.
   Web of Science ®Google Scholar
• Vijayaraghavan G, Sivakumar T, Kumar AV. Application of plant based coagulants for waste water treatment. Int J Adv Eng Res Stud. 2011;1(1):88–92.
   Google Scholar
• Jahn SAA. Using Moringa seeds as coagulants in developing countries. J‐Am Water Works Assoc. 1988;80(6):43–50.
   Web of Science ®Google Scholar
• Gandji K, Chadare F, Idohou R, et al. Status and utilisation of Moringa oleifera Lam: a review. Afr Crop Sci J. 2018;26(1):137–156.
   Google Scholar
• Mangale S, Chonde S, Jadhav A, et al. Study of Moringa oleifera (drumstick) seed as natural absorbent and antimicrobial agent for river water treatment. J Nat Prod Plant Resour. 2012;2(1):89–100.
   Google Scholar
• Karmakar A, Karmakar S, Mukherjee S. Properties of various plants and animals feedstocks for biodiesel production. Bioresour Technol. 2010;101(19):7201–7210.
   PubMed Web of Science ®Google Scholar
• Rashid U, Anwar F, Moser BR, et al. Moringa oleifera oil: a possible source of biodiesel. Bioresour Technol. 2008;99(17):8175–8179.
   PubMed Web of Science ®Google Scholar
• Ofor MO, Nwufo MI. The search for alternative energy sources: Jatropha and moringa seeds for biofuel production. J Agric Soc Res. 2011;11(2):87–94.
   Google Scholar
• da Silva JP, Serra TM, Gossmann M, et al. Moringa oleifera oil: studies of characterization and biodiesel production. Biomass Bioenergy. 2010;34(10):1527–1530.
   Web of Science ®Google Scholar
• Biswas W. Life cycle assessment of biodiesel production from Moringa oleifera oilseeds. Staff Publications - Conference Papers. Paris: Moringanews, Moringa and Plant Resources Network; 2008; 1–9.
   Google Scholar
• Rahman MM, Hassan MH, Kalam MA, et al. Performance and emission analysis of Jatropha curcas and Moringa oleifera methyl ester fuel blends in a multi-cylinder diesel engine. J Cleaner Prod. 2014;65:304–310.
   Web of Science ®Google Scholar
• Rashed M, Masjuki H, Kalam M, et al. Study of the oxidation stability and exhaust emission analysis of Moringa olifera biodiesel in a multi-cylinder diesel engine with aromatic amine antioxidants. Renew Energy. 2016;94:294–303.
   Web of Science ®Google Scholar
• Teoh YH, How HG, Masjuki HH, et al. Investigation on particulate emissions and combustion characteristics of a common-rail diesel engine fueled with Moringa oleifera biodiesel-diesel blends. Renew Energy. 2019;136:521–534.
   Web of Science ®Google Scholar
• Sastri B. The Wealth of India. A dictionary of Indian raw materials and industrial products raw materials, Vol. 6: LM. The Wealth of India A Dictionary of Indian Raw Materials and Industrial Products Raw Materials. New Delhi (India): Council of Scientific and Industrial Research; 1962; 6 LM.
   Google Scholar
• Varmani SG, Garg M. Health benefits of Moringa oleifera: a miracle tree. Int J Food Nutr Sci. 2014;3(3):111.
   Google Scholar
• Silver J. Moringa oleifera: the Future of Health. Village volunteers. 1–9.
   Google Scholar
• Emmanuel S, Zaku S, Adedirin S, et al. Moringa oleifera seed-cake, alternative biodegradable and biocompatibility organic fertilizer for modern farming. ABJNA. 2011;2(9):1289–1292.
   Google Scholar
• Exports R. Moringa seed cake. 2020 [cited 2021 Feb 22]. Available from: http://www.buymoringaplantproducts.com/moringa-seed-cake/
   Google Scholar
• Emmanuel S, Emmanuel B, Zaku S, et al. Biodiversity and agricultural productivity enhancement in Nigeria: application of processed Moringa oleifera seeds for improved organic farming. ABJNA. 2011;2(5):867–871.
   Google Scholar