10
Views
2
CrossRef citations to date
0
Altmetric
Original Articles

Genetic Instability of an Artificial Palindrom DNA Sequence

, &
Pages 729-742 | Received 10 Jun 1983, Published online: 21 May 2012

References and Footnotes

  • Kolter , R. and Helinski , D. R. 1979 . Ann. Rev. Genet. , 13 : 355 – 91 .
  • Maniatis , T. , Ptashne , M. , Backman , K. , Kleid , D. , Flashman , S. , Jeffrey , A. and Maurer , R. 1975 . Cell , 5 : 109 – 113 .
  • Rosenberg , M. and Court , D. 1979 . Ann. Rev. Genet. , 13 : 319 – 351 .
  • 1980 . Cold Spring Harbor Symp. Quant. Biol. , 43 “Movable Genetic Elements”, Vol. 1 and 2
  • Mizuuchi , K. , Mizuuchi , M. and Gellert , M. 1982 . J. Mol. Biol. , 156 : 229 – 243 .
  • Panayotatos , N. and Wells , R. D. 1982 . Nature , 289 : 466 – 470 .
  • Wells , R. D. , Goodman , T. C. , Hillen , W. , Horn , G. T. , Klein , R. D. , Larson , J. E. , Muller , U. R. , Neuendorf , S. K. , Panayotatos , N. and Stirdivant , S. M. 1980 . Prog. Nuc. Acid. Res. and Mol. Biology , 24 : 167 – 267 .
  • Albertini , A. M. , Hefer , M. , Calos , M. P. and Miller , J. H. 1982 . Cell , 29 : 319 – 328 .
  • McClintock , B. Cold Spring Harbor Symp. Quant. Biol. , 16 13 – 57 . (1952)
  • Crains , J. 1981 . Nature , 289 : 353 – 357 .
  • Klein , G. 1981 . Nature , 294 : 313 – 318 .
  • Sutcliffe , J. G. Cold Spring Harbor Symp. Quant. Biol. , 43 77 – 90 . (1978)
  • Kolter , R. and Helinski , D. R. 1978 . J. Mol. Biol. , 124 : 425 – 441 .
  • Inuzuka , N. , Inuzuka , M. and Helinski , D. R. 1980 . J. Biol. Chem. , 255 : 11071 – 11074 . Crosa, J.H. ibid. 255, 11075–11077 (1980)
  • Kolter , R. , Inuzuka , M. and Helinski , D. R. 1978 . Cell , 15 : 1199 – 1208 .
  • Stalker , D. M. , Kolter , R. and Helinski , D. R. 1982 . J. Mol. Biol. , 161 : 33 – 34 .
  • Shafferman , A. and Helinski , D. R. 1983 . J. Biol. Chem. , 258 : 4083 – 4091 .
  • Kolter , R. and Helinski , D. R. 1978 . Plasmid , 1 : 571 – 580 .
  • Shafferman , A. , Stalker , D. M. , Tolun , A. , Kolter , R. and Helinski , D. R. 1981 . Mol. Biol. Pathogenicity & Ecology of Bad. Plasmids. Edited by: Levy , S. B. , Clowes , R. C. and Koenig , E. L. 259 – 269 . Plenum Pub. Co
  • We are currently engaged in the sequencing of these mutants to pin point exactly the nucleotides deleted
  • Farabough , P. J. , Scheissner , V. , Hofer , M. and Miller , J. H. 1978 . J. Mol. Biol. , 126 : 847 – 857 .
  • Edluned , T. and Normark , S. 1981 . Nature , 292 : 269 – 271 .
  • McKorkle , G. M. and Altman , S. 1982 . J. Mol. Biol. , 155 : 83 – 103 .
  • Streisinger , G. , Okada , Y. , Emich , J. , Newton , J. , Tsugita , A. , Terzaghi , E. and Inoye , M. 1966 . Cold Spring Harbor Symp. Quant. Biol. , 31 : 77 – 84 .
  • Kolter , R. and Helinski , D. R. 1982 . J. Mol. Biol. , 161 : 45 – 56 .
  • Inuzuka , M. and Helinski , D. R. 1978 . Biochemistry , 17 : 2567 – 2573 .
  • Shafferman , A. , Kolter , R. , Stalker , D. M. and Helinski , D. R. 1982 . J. Mol. Biol. , 161 : 57 – 76 .
  • Germino , J. and Bastia , D. 1983 . Cell , 32 : 131 – 140 .
  • Jones , I. M. , Primrose , S. B. and Ehrlich , S. D. 1982 . Mol. Gen. Genet. , 188 : 486 – 489 .
  • Collins . 1980 . J. Cold Spring Harbor Symp. Quant. Biol. , 45 : 409 – 416 .
  • Masamune , Y. and Richardson , C. C. 1964 . J. Biol. Chem. , 239 : 233 – 241 .
  • Enger , C. and Bery , D. E. 1981 . Proc. Nat. Acad. Sci. U.S.A. , 78 : 459 – 463 .
  • It is possible that the insertion of the 58 bp palindrom in locations other than that of pAS807 may also be unstable. If for example the incidence of palindrom instability in other locations is 10−5instead of IO−4 per round of plasmid DNA replication, we would have to increase either the number of clones to be analyzed from 20–30 to 200–300 clones or to increase the number of generations by a factor of 10. The latter approach is now being used to test all the palindrom insertions considered to be stable
  • Patient , R. K. , Hardies , S. C. , Larson , J. E. , Inman , R. B. , Maquat , L. E. and Wells , R. D. 1979 . J. Biol. Chem. , 254 : 5548 – 5558 .
  • Efstratiadis , A. , Posakony , J. W. , Maniatis , T. , Lawn , R. M. , O'Connell , C. , Spritz , R. A. , De Riel , J. K. , Forget , B. G. , Weissman , S. M. , Slightom , J. L. , Blachl , A. E. , Smithies , O. , Baralle , F. E. , Shoulders , C. C. and Proudfoot , N. J. 1980 . Cell , 21 : 653 – 668 .
  • Chang , C. Y. and Cohen , N. 1978 . J. Bact. , 134 : 1141 – 1156 .
  • Hershfield , V. , Boyer , H. W. , Chow , L. and Helinski , D. R. 1976 . J. Bact. , 126 : 447 – 453 .

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.