Identification of novel T-cell epitopes on monkeypox virus and development of multi-epitopes vaccine using immunoinformatics approaches

References

• Abraham, M. J., Murtola, T., Schulz, R., Páll, S., Smith, J. C., Hess, B., & Lindahl, E. (2015). GROMACS: High performance molecular simulations through multi-level parallelism from laptops to supercomputers. SoftwareX, 1-2, 19–25. https://doi.org/10.1016/j.softx.2015.06.001
   Google Scholar
• Aebig, J. A., Mullen, G. E. D., Dobrescu, G., Rausch, K., Lambert, L., Ajose-Popoola, O., Long, C. A., Saul, A., & Miles, A. P. (2007). Formulation of vaccines containing CpG oligonucleotides and alum. Journal of Immunological Methods, 323(2), 139–146.https://doi.org/10.1016/j.jim.2007.04.003
   PubMed Web of Science ®Google Scholar
• Aier, I., Varadwaj, P. K., & Raj, U. (2016). Structural insights into conformational stability of both wild-type and mutant EZH2 receptor. Scientific Reports, 6, 34984. https://doi.org/10.1038/srep34984
   PubMed Web of Science ®Google Scholar
• Alakunle, E., Moens, U., Nchinda, G., & Okeke, M. I. (2020). Monkeypox virus in Nigeria: Infection biology, epidemiology, and evolution. Viruses, 12(11), 1257. https://doi.org/10.3390/v12111257
   PubMed Web of Science ®Google Scholar
• Bhattacharya, K., Shamkh, I. M., Khan, M. S., Lotfy, M. M., Nzeyimana, J. B., Abutayeh, R. F., Hamdy, N. M., Hamza, D., Chanu, N. R., Khanal, P., Bhattacharjee, A., & Basalious, E. B. (2022). Multi-epitope vaccine design against monkeypox virus via reverse vaccinology method exploiting immunoinformatic and bioinformatic approaches. Vaccines, 10(12), 2010. https://doi.org/10.3390/vaccines10122010
   Web of Science ®Google Scholar
• Biasini, M., Bienert, S., Waterhouse, A., Arnold, K., Studer, G., Schmidt, T., Kiefer, F., Gallo Cassarino, T., Bertoni, M., Bordoli, L., & Schwede, T. (2014). SWISS-MODEL: Modelling protein tertiary and quaternary structure using evolutionary information. Nucleic Acids Research, 42(Web Server issue), W252–258. https://doi.org/10.1093/nar/gku340
   PubMed Web of Science ®Google Scholar
• Buchman, G. W., Cohen, M. E., Xiao, Y., Richardson-Harman, N., Silvera, P., DeTolla, L. J., Davis, H. L., Eisenberg, R. J., Cohen, G. H., & Isaacs, S. N. (2010). A protein-based smallpox vaccine protects non-human primates from a lethal monkeypox virus challenge. Vaccine, 28(40), 6627–6636. https://doi.org/10.1016/j.vaccine.2010.07.030
   PubMed Web of Science ®Google Scholar
• Bui, H.-H., Sidney, J., Dinh, K., Southwood, S., Newman, M. J., & Sette, A. (2006). Predicting population coverage of T-cell epitope-based diagnostics and vaccines. BMC Bioinformatics, 7(1), 153. https://doi.org/10.1186/1471-2105-7-153
   PubMed Web of Science ®Google Scholar
• Bunge, E. M., Hoet, B., Chen, L., Lienert, F., Weidenthaler, H., Baer, L. R., & Steffen, R. (2022). The changing epidemiology of human monkeypox-A potential threat? A systematic review. PLoS Neglected Tropical Diseases, 16(2), e0010141. https://doi.org/10.1371/journal.pntd.0010141
   PubMed Web of Science ®Google Scholar
• Calis, J. J. A., Maybeno, M., Greenbaum, J. A., Weiskopf, D., De Silva, A. D., Sette, A., Keşmir, C., & Peters, B. (2013). Properties of MHC Class I presented peptides that enhance immunogenicity. PLoS Computational Biology, 9(10), e1003266. https://doi.org/10.1371/journal.pcbi.1003266
   PubMed Web of Science ®Google Scholar
• Centers for Disease Control and Prevention. (2022). Monkeypox—Vaccine guidance. https://www.cdc.gov/poxvirus/monkeypox/clinicians/smallpox-vaccine.html
   Google Scholar
• Chatterjee, N., Ojha, R., Khatoon, N., & Prajapati, V. K. (2018). Scrutinizing Mycobacterium tuberculosis membrane and secretory proteins to formulate multiepitope subunit vaccine against pulmonary tuberculosis by utilizing immunoinformatic approaches. International Journal of Biological Macromolecules, 118(Pt A), 180–188. https://doi.org/10.1016/j.ijbiomac.2018.06.080
   PubMedGoogle Scholar
• Chen, X., Zaro, J. L., & Shen, W.-C. (2013). Fusion protein linkers: Property, design and functionality. Advanced Drug Delivery Reviews, 65(10), 1357–1369. https://doi.org/10.1016/j.addr.2012.09.039
   PubMed Web of Science ®Google Scholar
• Cheng, J., Randall, A. Z., Sweredoski, M. J., & Baldi, P. (2005). SCRATCH: A protein structure and structural feature prediction server. Nucleic Acids Research, 33(Web Server issue), W72–76. https://doi.org/10.1093/nar/gki396
   PubMedGoogle Scholar
• Colovos, C., & Yeates, T. O. (1993). Verification of protein structures: Patterns of nonbonded atomic interactions. Protein Science: A Publication of the Protein Society, 2(9), 1511–1519. https://doi.org/10.1002/pro.5560020916
   PubMed Web of Science ®Google Scholar
• Cooper, C. L., Davis, H. L., Angel, J. B., Morris, M. L., Elfer, S. M., Seguin, I., Krieg, A. M., & Cameron, D. W. (2005). CPG 7909 adjuvant improves hepatitis B virus vaccine seroprotection in antiretroviral-treated HIV-infected adults. AIDS (London, England), 19(14), 1473–1479. https://doi.org/10.1097/01.aids.0000183514.37513.d2
   PubMed Web of Science ®Google Scholar
• Cooper, C. L., Davis, H. L., Morris, M. L., Efler, S. M., Adhami, M. A., Krieg, A. M., Cameron, D. W., & Heathcote, J. (2004). CPG 7909, an immunostimulatory TLR9 agonist oligodeoxynucleotide, as adjuvant to Engerix-B HBV vaccine in healthy adults: A double-blind phase I/II study. Journal of Clinical Immunology, 24(6), 693–701. https://doi.org/10.1007/s10875-004-6244-3
   PubMed Web of Science ®Google Scholar
• De Gregorio, E., Caproni, E., & Ulmer, J. B. (2013). Vaccine adjuvants: Mode of action. Frontiers in Immunology, 4, 214. https://doi.org/10.3389/fimmu.2013.00214
   PubMed Web of Science ®Google Scholar
• Dimitrov, I., Bangov, I., Flower, D. R., & Doytchinova, I. (2014). AllerTOP v.2—A server for in silico prediction of allergens. Journal of Molecular Modeling, 20(6), 2278.https://doi.org/10.1007/s00894-014-2278-5
   PubMed Web of Science ®Google Scholar
• Ezzemani, W., Kettani, A., Sappati, S., Kondaka, K., El Ossmani, H., Tsukiyama-Kohara, K., Altawalah, H., Saile, R., Kohara, M., Benjelloun, S., & Ezzikouri, S. (2023). Reverse vaccinology-based prediction of a multi-epitope SARS-CoV-2 vaccine and its tailoring to new coronavirus variants. Journal of Biomolecular Structure and Dynamics, 41(11), 4917–4938. https://doi.org/10.1080/07391102.2022.2075468
   PubMed Web of Science ®Google Scholar
• Ezzemani, W., Kettani, A., Sappati, S., Kondaka, K., El Ossmani, H., Tsukiyama-Kohara, K., Altawalah, H., Saile, R., Kohara, M., Benjelloun, S., & Ezzikouri, S. (2023). Design of a multi-epitope Zika virus vaccine candidate—An in-silico study. Journal of Biomolecular Structure and Dynamics, 41, 3762–3771. https://doi.org/10.1080/07391102.2022.2055648
   Google Scholar
• Ezzemani, W., Windisch, M. P., Kettani, A., Altawalah, H., Nourlil, J., Benjelloun, S., & Ezzikouri, S. (2021). Immuno-informatics-based identification of novel potential B cell and T cell epitopes to fight zika virus infections. Infectious Disorders Drug Targets, 21(4), 572–581. https://doi.org/10.2174/1871526520666200810153657
   PubMedGoogle Scholar
• Fleri, W., Paul, S., Dhanda, S. K., Mahajan, S., Xu, X., Peters, B., & Sette, A. (2017). The immune epitope database and analysis resource in epitope discovery and synthetic vaccine design. Frontiers in Immunology, 8, 278. https://doi.org/10.3389/fimmu.2017.00278
   PubMed Web of Science ®Google Scholar
• Gorai, S., Das, N. C., Gupta, P. S. S., Panda, S. K., Rana, M. K., & Mukherjee, S. (2022). Designing efficient multi-epitope peptide-based vaccine by targeting the antioxidant thioredoxin of bancroftian filarial parasite. Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases, 98, 105237. https://doi.org/10.1016/j.meegid.2022.105237
   PubMed Web of Science ®Google Scholar
• Grote, A., Hiller, K., Scheer, M., Münch, R., Nörtemann, B., Hempel, D. C., & Jahn, D. (2005). JCat: A novel tool to adapt codon usage of a target gene to its potential expression host. Nucleic Acids Research, 33(Web Server issue), W526–531. https://doi.org/10.1093/nar/gki376
   PubMed Web of Science ®Google Scholar
• Gupta, P. S. S., Bhat, H. R., Biswal, S., & Rana, M. K. (2020). Computer-aided discovery of bis-indole derivatives as multi-target drugs against cancer and bacterial infections: DFT, docking, virtual screening, and molecular dynamics studies. Journal of Molecular Liquids, 320, 114375. https://doi.org/10.1016/j.molliq.2020.114375
   Web of Science ®Google Scholar
• Gupta, S., Kapoor, P., Chaudhary, K., Gautam, A., Kumar, R., & Raghava, G. P. S. (2015). Peptide toxicity prediction. Methods in Molecular Biology (Clifton, N.J.), 1268, 143–157. https://doi.org/10.1007/978-1-4939-2285-7_7
   PubMedGoogle Scholar
• Gupta, S., Kapoor, P., Chaudhary, K., Gautam, A., Kumar, R., Open Source Drug Discovery Consortium, & Raghava, G. P. S. (2013). In silico approach for predicting toxicity of peptides and proteins. PLoS ONE, 8(9), e73957. https://doi.org/10.1371/journal.pone.0073957
   PubMed Web of Science ®Google Scholar
• Heegaard, P. M. H., Dedieu, L., Johnson, N., Le Potier, M.-F., Mockey, M., Mutinelli, F., Vahlenkamp, T., Vascellari, M., & Sørensen, N. S. (2011). Adjuvants and delivery systems in veterinary vaccinology: Current state and future developments. Archives of Virology, 156(2), 183–202. https://doi.org/10.1007/s00705-010-0863-1
   PubMed Web of Science ®Google Scholar
• Heo, L., Park, H., & Seok, C. (2013). GalaxyRefine: Protein structure refinement driven by side-chain repacking. Nucleic Acids Research, 41(Web Server issue), W384–388. https://doi.org/10.1093/nar/gkt458
   PubMed Web of Science ®Google Scholar
• Isidro, J., Borges, V., Pinto, M., Ferreira, R., Sobral, D.,, Nunes, A., Santos, J. D., Borrego, M. J., Núncio, S., Pelerito, A., Cordeiro, R., & Gomes, J. P. (2022). First draft genome sequene of Monkeypox virus associated with the suspected multi-country outbreak, May 2022 (Confirmed case in Portugal). Retrieved May 23, 2022, from https://virological.org/t/first-draft-genome-sequence-of-monkeypox-virus-associated-with-the-suspected-multi-country-outbreak-may-2022-confirmed-case-in-portugal/799
   Google Scholar
• Islam, M. R., Hossain, M. J., Roy, A., Hasan, A. H. M. N., Rahman, M. A., Shahriar, M., & Bhuiyan, M. A. (2022). Repositioning potentials of smallpox vaccines and antiviral agents in monkeypox outbreak: A rapid review on comparative benefits and risks. Health Science Reports, 5(5), e798. https://doi.org/10.1002/hsr2.798
   PubMedGoogle Scholar
• Israely, T., Melamed, S., Achdout, H., Erez, N., Politi, B., Waner, T., Lustig, S., & Paran, N. (2014). TLR3 and TLR9 agonists improve postexposure vaccination efficacy of live smallpox vaccines. PloS One, 9(10), e110545. https://doi.org/10.1371/journal.pone.0110545
   PubMed Web of Science ®Google Scholar
• Jaydari, A., Nazifi, N., & Forouharmehr, A. (2020). Computational design of a novel multi-epitope vaccine against Coxiella burnetii. Human Immunology, 81(10-11), 596–605. https://doi.org/10.1016/j.humimm.2020.05.010
   PubMed Web of Science ®Google Scholar
• Jurk, M., Schulte, B., Kritzler, A., Noll, B., Uhlmann, E., Wader, T., Schetter, C., Krieg, A. M., & Vollmer, J. (2004). C-Class CpG ODN: Sequence requirements and characterization of immunostimulatory activities on mRNA level. Immunobiology, 209(1–2), 141–154. https://doi.org/10.1016/j.imbio.2004.02.006
   PubMed Web of Science ®Google Scholar
• Kar, T., Narsaria, U., Basak, S., Deb, D., Castiglione, F., Mueller, D. M., & Srivastava, A. P. (2020). A candidate multi-epitope vaccine against SARS-CoV-2. Scientific Reports, 10(1), 10895.https://doi.org/10.1038/s41598-020-67749-1
   PubMed Web of Science ®Google Scholar
• Kim, S. K., Ragupathi, G., Cappello, S., Kagan, E., & Livingston, P. O. (2000). Effect of immunological adjuvant combinations on the antibody and T-cell response to vaccination with MUC1-KLH and GD3-KLH conjugates. Vaccine, 19(4–5), 530–537. https://doi.org/10.1016/s0264-410x(00)00195-x
   PubMed Web of Science ®Google Scholar
• Ko, J., Park, H., Heo, L., & Seok, C. (2012). GalaxyWEB server for protein structure prediction and refinement. Nucleic Acids Research, 40(Web Server issue), W294–297. https://doi.org/10.1093/nar/gks493
   PubMed Web of Science ®Google Scholar
• Kovacs, J. A., Chacón, P., & Abagyan, R. (2004). Predictions of protein flexibility: First-order measures. Proteins: Structure, Function, and Bioinformatics, 56(4), 661–668. https://doi.org/10.1002/prot.20151
   Google Scholar
• Krieg, A. M. (2006). Therapeutic potential of Toll-like receptor 9 activation. Nature Reviews Drug Discovery, 5(6), 471–484. https://doi.org/10.1038/nrd2059
   PubMed Web of Science ®Google Scholar
• Kwissa, M., Amara, R. R., Robinson, H. L., Moss, B., Alkan, S., Jabbar, A., Villinger, F., & Pulendran, B. (2007). Adjuvanting a DNA vaccine with a TLR9 ligand plus Flt3 ligand results in enhanced cellular immunity against the simian immunodeficiency virus. The Journal of Experimental Medicine, 204(11), 2733–2746. https://doi.org/10.1084/jem.20071211
   PubMed Web of Science ®Google Scholar
• Laskowski, R. A., Rullmannn, J. A., MacArthur, M. W., Kaptein, R., & Thornton, J. M. (1996). AQUA and PROCHECK-NMR: Programs for checking the quality of protein structures solved by NMR. Journal of Biomolecular NMR, 8(4), 477–486. https://doi.org/10.1007/BF00228148
   PubMed Web of Science ®Google Scholar
• Laskowski, R. A. (2009). PDBsum new things. Nucleic Acids Research, 37(Database issue), D355–359. https://doi.org/10.1093/nar/gkn860
   PubMed Web of Science ®Google Scholar
• Li, Y., Olson, V. A., Laue, T., Laker, M. T., & Damon, I. K. (2006). Detection of monkeypox virus with real-time PCR assays. Journal of Clinical Virology: The Official Publication of the Pan American Society for Clinical Virology, 36(3), 194–203. https://doi.org/10.1016/j.jcv.2006.03.012
   PubMed Web of Science ®Google Scholar
• Lim, H. X., Lim, J., Jazayeri, S. D., Poppema, S., & Poh, C. L. (2021). Development of multi-epitope peptide-based vaccines against SARS-CoV-2. Biomedical Journal, 44(1), 18–30. https://doi.org/10.1016/j.bj.2020.09.005
   PubMed Web of Science ®Google Scholar
• Lim, H. X., Lim, J., & Poh, C. L. (2021). Identification and selection of immunodominant B and T cell epitopes for dengue multi-epitope-based vaccine. Medical Microbiology and Immunology, 210(1), 1–11. https://doi.org/10.1007/s00430-021-00700-x
   PubMed Web of Science ®Google Scholar
• Lopéz-Blanco, J. R., Garzón, J. I., & Chacón, P. (2011). iMod: Multipurpose normal mode analysis in internal coordinates. Bioinformatics (Oxford, England), 27(20), 2843–2850. https://doi.org/10.1093/bioinformatics/btr497
   PubMed Web of Science ®Google Scholar
• López-Blanco, J. R., Aliaga, J. I., Quintana-Ortí, E. S., & Chacón, P. (2014). iMODS: Internal coordinates normal mode analysis server. Nucleic Acids Research, 42(Web Server issue), W271–276. https://doi.org/10.1093/nar/gku339
   PubMed Web of Science ®Google Scholar
• Macneil, A., Reynolds, M. G., Braden, Z., Carroll, D. S., Bostik, V., Karem, K., Smith, S. K., Davidson, W., Li, Y., Moundeli, A., Mombouli, J.-V., Jumaan, A. O., Schmid, D. S., Regnery, R. L., & Damon, I. K. (2009). Transmission of atypical varicella-zoster virus infections involving palm and sole manifestations in an area with monkeypox endemicity. Clinical Infectious Diseases : An Official Publication of the Infectious Diseases Society of America, 48(1), e6-8–e8. https://doi.org/10.1086/595552
   PubMed Web of Science ®Google Scholar
• Nezafat, N., Ghasemi, Y., Javadi, G., Khoshnoud, M. J., & Omidinia, E. (2014). A novel multi-epitope peptide vaccine against cancer: An in silico approach. Journal of Theoretical Biology, 349, 121–134. https://doi.org/10.1016/j.jtbi.2014.01.018
   PubMed Web of Science ®Google Scholar
• Nielsen, M., & Lund, O. (2009). NN-align. An artificial neural network-based alignment algorithm for MHC class II peptide binding prediction. BMC Bioinformatics, 10, 296.https://doi.org/10.1186/1471-2105-10-296
   PubMed Web of Science ®Google Scholar
• Ohto, U., Shibata, T., Tanji, H., Ishida, H., Krayukhina, E., Uchiyama, S., Miyake, K., & Shimizu, T. (2015). Structural basis of CpG and inhibitory DNA recognition by Toll-like receptor 9. Nature, 520(7549), 702–705. https://doi.org/10.1038/nature14138
   PubMed Web of Science ®Google Scholar
• Pandey, R. K., Ojha, R., Aathmanathan, V. S., Krishnan, M., & Prajapati, V. K. (2018). Immunoinformatics approaches to design a novel multi-epitope subunit vaccine against HIV infection. Vaccine, 36(17), 2262–2272. https://doi.org/10.1016/j.vaccine.2018.03.042
   PubMed Web of Science ®Google Scholar
• Pal, M., Mengstie, F., & Kandi, V. (2017). Epidemiology, diagnosis, and control of monkeypox disease: A comprehensive review. American Journal of Infectious Diseases and Microbiology, 5(2), 94–99. https://doi.org/10.12691/ajidm-5-2-4
   Google Scholar
• Pauling, L., Corey, R. B., & Branson, H. R. (1951). The structure of proteins; two hydrogen-bonded helical configurations of the polypeptide chain. Proceedings of the National Academy of Sciences of the United States of America, 37(4), 205–211. https://doi.org/10.1073/pnas.37.4.205
   PubMed Web of Science ®Google Scholar
• Mahmud, S., Rafi, M. O., Paul, G. K., Promi, M. M., Shimu, M. S. S., Biswas, S., Emran, T. B., Dhama, K., Alyami, S. A., Moni, M. A., & Saleh, M. A. (2021). Designing a multi-epitope vaccine candidate to combat MERS-CoV by employing an immunoinformatics approach. Scientific Reports, 11(1), 15431. https://doi.org/10.1038/s41598-021-92176-1
   PubMed Web of Science ®Google Scholar
• Moss, B. (2013). Poxvirus DNA replication. Cold Spring Harbor Perspectives in Biology, 5(9), a010199–a010199. https://doi.org/10.1101/cshperspect.a010199
   PubMed Web of Science ®Google Scholar
• Palatnik-de-Sousa, C. B., Soares, I. d S., & Rosa, D. S. (2018). Editorial: Epitope discovery and synthetic vaccine design. Frontiers in Immunology, 9, 826. https://doi.org/10.3389/fimmu.2018.00826
   PubMed Web of Science ®Google Scholar
• Panda, S. K., Saxena, S., & Guruprasad, L. (2020). Homology modeling, docking and structure-based virtual screening for new inhibitor identification of Klebsiella pneumoniae heptosyltransferase-III. Journal of Biomolecular Structure & Dynamics, 38(7), 1887–1902. https://doi.org/10.1080/07391102.2019.1624296
   PubMed Web of Science ®Google Scholar
• Parker, S., & Buller, R. M. (2013). A review of experimental and natural infections of animals with monkeypox virus between 1958 and 2012. Future Virology, 8(2), 129–157. https://doi.org/10.2217/fvl.12.130
   PubMed Web of Science ®Google Scholar
• Pauli, G., Blümel, J., Burger, R., Drosten, C., Gröner, A., Gürtler, L., Heiden, M., Hildebrandt, M., Jansen, B., Montag-Lessing, T., Offergeld, R., Seitz, R., Schlenkrich, U., Schottstedt, V., Strobel, J., Willkommen, H., & von König, C.-H W. (2010). Orthopox viruses: Infections in humans. Transfusion Medicine and Hemotherapy: Offizielles Organ Der Deutschen Gesellschaft Fur Transfusionsmedizin Und Immunhamatologie, 37(6), 351–364. https://doi.org/10.1159/000322101
   PubMed Web of Science ®Google Scholar
• Quarleri, J., Delpino, M. V., & Galvan, V. (2022). Monkeypox: Considerations for the understanding and containment of the current outbreak in non-endemic countries. GeroScience, 44(4), 2095–2103. https://doi.org/10.1007/s11357-022-00611-6
   PubMed Web of Science ®Google Scholar
• Rao, A. K., Schulte, J., Chen, T.-H., Hughes, C. M., Davidson, W., Neff, J. M., Markarian, M., Delea, K. C., Wada, S., Liddell, A., Alexander, S., Sunshine, B., Huang, P., Honza, H. T., Rey, A., Monroe, B., Doty, J., Christensen, B., Delaney, L., … McCollum, A. M. (2022). Monkeypox in a traveler returning from Nigeria—Dallas, Texas, July 2021. MMWR. Morbidity and Mortality Weekly Report, 71(14), 509–516. https://doi.org/10.15585/mmwr.mm7114a1
   PubMed Web of Science ®Google Scholar
• Reynisson, B., Alvarez, B., Paul, S., Peters, B., & Nielsen, M. (2020). NetMHCpan-4.1 and NetMHCIIpan-4.0: Improved predictions of MHC antigen presentation by concurrent motif deconvolution and integration of MS MHC eluted ligand data. Nucleic Acids Research, 48(W1), W449–W454. https://doi.org/10.1093/nar/gkaa379
   PubMed Web of Science ®Google Scholar
• Rosano, G. L., & Ceccarelli, E. A. (2014). Recombinant protein expression in Escherichia coli: Advances and challenges. Frontiers in Microbiology, 5, 172. https://doi.org/10.3389/fmicb.2014.00172
   PubMed Web of Science ®Google Scholar
• Russell, C. D., Unger, S. A., Walton, M., & Schwarze, J. (2017). The human immune response to respiratory syncytial virus infection. Clinical Microbiology Reviews, 30(2), 481–502. https://doi.org/10.1128/CMR.00090-16
   PubMed Web of Science ®Google Scholar
• Saghazadeh, A., & Rezaei, N. (2022). Poxviruses and the immune system: Implications for monkeypox virus. International Immunopharmacology, 113(Pt A), 109364. https://doi.org/10.1016/j.intimp.2022.109364
   PubMedGoogle Scholar
• Sah, R., Abdelaal, A., Reda, A., Katamesh, B. E., Manirambona, E., Abdelmonem, H., Mehta, R., Rabaan, A. A., Alhumaid, S., Alfouzan, W. A., Alomar, A. I., Khamis, F., Alofi, F. S., Aljohani, M. H., Alfaraj, A. H., Alfaresi, M., Al-Jishi, J. M., Alsalman, J., Alynbiawi, A., Almogbel, M. S., & Rodriguez-Morales, A. J. (2022). Monkeypox and its possible sexual transmission: Where are we now with its evidence? Pathogens, 11(8), 924. https://doi.org/10.3390/pathogens11080924
   PubMed Web of Science ®Google Scholar
• Sanches, R. C. O., Tiwari, S., Ferreira, L. C. G., Oliveira, F. M., Lopes, M. D., Passos, M. J. F., Maia, E. H. B., Taranto, A. G., Kato, R., Azevedo, V. A. C., & Lopes, D. O. (2021). Immunoinformatics design of multi-epitope peptide-based vaccine against schistosoma mansoni using transmembrane proteins as a target. Frontiers in Immunology, 12, 621706. https://doi.org/10.3389/fimmu.2021.621706
   PubMed Web of Science ®Google Scholar
• Shchelkunov, S. N., & Shchelkunova, G. A. (2020). Genes that control vaccinia virus immunogenicity. Acta Naturae, 12(1), 33–41. https://doi.org/10.32607/actanaturae.10935
   PubMed Web of Science ®Google Scholar
• Song, H.-S., Park, S., Huh, J.-W., Lee, Y.-R., Jung, D.-J., Yang, C., Kim, S. H., Kim, H. M., & Kim, Y.-M. (2022). N-glycosylation of UNC93B1 at a specific asparagine residue is required for TLR9 signaling. Frontiers in Immunology, 13, 875083. https://doi.org/10.3389/fimmu.2022.875083
   PubMed Web of Science ®Google Scholar
• Sørensen, H. P., & Mortensen, K. K. (2005). Soluble expression of recombinant proteins in the cytoplasm of Escherichia coli. Microbial Cell Factories, 4(1), 1. https://doi.org/10.1186/1475-2859-4-1
   PubMed Web of Science ®Google Scholar
• Ullah, A., Shahid, F. A., Haq, M. U., Tahir Ul Qamar, M., Irfan, M., Shaker, B., Ahmad, S., Alrumaihi, F., Allemailem, K. S., & Almatroudi, A. (2022). An integrative reverse vaccinology, immunoinformatic, docking and simulation approaches towards designing of multi-epitopes based vaccine against monkeypox virus. Journal of Biomolecular Structure & Dynamics, 1–14.https://doi.org/10.1080/07391102.2022.2125441
   Web of Science ®Google Scholar
• Vajda, S., Yueh, C., Beglov, D., Bohnuud, T., Mottarella, S. E., Xia, B., Hall, D. R., & Kozakov, D. (2017). New additions to the ClusPro server motivated by CAPRI. Proteins, 85(3), 435–444. https://doi.org/10.1002/prot.25219
   PubMed Web of Science ®Google Scholar
• Vaughan, A. M., Cenciarelli, O., Colombe, S., Alves de Sousa, L., Fischer, N., Gossner, C. M., Pires, J., Scardina, G., Aspelund, G., Avercenko, M., Bengtsson, S., Blomquist, P., Caraglia, A., Chazelle, E., Cohen, O., Diaz, A., Dillon, C., Dontsenko, I., Kotkavaara, K., … Haussig, J. M. (2022). A large multi-country outbreak of monkeypox across 41 countries in the WHO European Region, 7 March to 23 August 2022. Eurosurveillance, 27(36), 2200620. https://doi.org/10.2807/1560-7917.ES.2022.27.36.2200620
   Web of Science ®Google Scholar
• WHO. (2022). Monkeypox: 19 May 2022. Retrieved October 29https://www.who.int/news-room/fact-sheets/detail/monkeypox
   Google Scholar
• WHO. (2022). Multi-country monkeypox outbreak in non-endemic countries: 21 May 2022. Retrieved October 29, https://www.who.int/emergencies/disease-outbreak-news/item/2022-DON385
   Google Scholar
• WHO. (2022). Monkeypox. https://www.who.int/news-room/fact-sheets/detail/monkeypox
   Google Scholar
• Wiederstein, M., & Sippl, M. J. (2007). ProSA-web: Interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Research, 35(Web Server issue), W407–410. https://doi.org/10.1093/nar/gkm290
   PubMed Web of Science ®Google Scholar
• Wilkins, M. R., Gasteiger, E., Bairoch, A., Sanchez, J.-C., Williams, K. L., Appel, R. D., & Hochstrasser, D. F. (1998). Protein identification and analysis tools in the ExPASy server. In A. J. Link (Ed.), 2-D Proteome analysis protocols (Vol. 112, pp. 531–552). Humana Press. https://doi.org/10.1385/1-59259-584-7:531
   Google Scholar
• Xiao, Y., Zeng, Y., Schante, C., Joshi, S. B., Buchman, G. W., Volkin, D. B., Middaugh, C. R., & Isaacs, S. N. (2020). Short-term and longer-term protective immune responses generated by subunit vaccination with smallpox A33, B5, L1 or A27 proteins adjuvanted with aluminum hydroxide and CpG in mice challenged with vaccinia virus. Vaccine, 38(38), 6007–6018. https://doi.org/10.1016/j.vaccine.2020.07.018
   PubMed Web of Science ®Google Scholar
• Yu, M., Zhu, Y., Li, Y., Chen, Z., Sha, T., Li, Z., Zhang, F., & Ding, J. (2021). Design of a novel multi-epitope vaccine against echinococcus granulosus in immunoinformatics. Frontiers in Immunology, 12, 668492. https://doi.org/10.3389/fimmu.2021.668492
   PubMed Web of Science ®Google Scholar
• Zaharieva, N., Dimitrov, I., Flower, D. R., & Doytchinova, I. (2019). VaxiJen dataset of bacterial immunogens: An update. Current Computer-Aided Drug Design, 15(5), 398–400. https://doi.org/10.2174/1573409915666190318121838
   PubMed Web of Science ®Google Scholar
• Zaib, S., Rana, N., Areeba, null, Hussain, N., Alrbyawi, H., Dera, A. A., Khan, I., Khalid, M., Khan, A., & Al-Harrasi, A. (2023). Designing multi-epitope monkeypox virus-specific vaccine using immunoinformatics approach. Journal of Infection and Public Health, 16(1), 107–116. https://doi.org/10.1016/j.jiph.2022.11.033
   PubMed Web of Science ®Google Scholar