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Original Articles

Overview of Marine Toxin Research in Cuba

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Pages 117-136 | Published online: 15 Sep 2010

  • Gomez , T. , Romero , D. L. , Wang , L. , Barrai , A. M. , Marí'nez , J. R. and Chávez , M. A. 1986 . Isolation and partial purification of two toxins and a protease inhibitor from Stoichactis helianthus anemone . Revista Cubana de Investigaciones Biomedicas , 5 : 117 – 125 . (in Spanish}
  • Lanio , M. E. , Morera , V. , Alvarez , C. , Tejuca , M. , Gomez , T. , Pazos , P. , Besada , V. , Martinez , D. , Huerta , V. , Padron , G. and Chavez , M. A. 2001 . Purification and characterization of two hemolysins from Stichodactyla helianthus . Toxicon , 39 : 187 – 194 .
  • Aneiros , A. , Garcia , I. , Martinez , J. R. , Harvey , A. L. , Anderson , A. J. , Marshall , D. L. , Engstrom , A. , Hellman , U. and Karlsson , E. 1993 . A potassium channel toxin from the secretion of the sea anemone Bunodosoma granultfera. Isolation, amino acid sequence and biological activity . Biochimica et Biophysica Ada , 71157 : 86 – 92 .
  • Salinas , E. M. , Cebada , J. , Valdes , A. , Garateix , A. , Aneiros , A. and Alvarez , J. L. 1997 . Effects of a toxin from the mucus of the Caribbean sea anemone (Bunodosoma gnmulifera} on the ionic currents of single ventricular mammalian cardiomyocytes . Toxicon , 35 : 1699 – 1709 .
  • Garateix , A. , Castellanos , M. , Hemndez , I. , Mas , R. , Romero , L. and Chavez , M. 1992 . Effects of a high molecular weight toxin from the sea anemone Condylactis gigantea on cholinergic responses . Comparative Biochemistry and Physiology , 103 : 403 – 409 .
  • Romero , L. 1997 . “ Isolation and purification of toxins from the sea anemone Condylactis gigantea ” . Cuba : Biochemistry Department, University of Havana . Master in science thesis. (in Spanish}
  • Garateix , A. , Flores , A. , Garcia-Andrade , J. M. , Palmero , A. , Aneiros , A. , Vega , R. and Soto , E. 1996 . Antagonism of glutamate receptors by a Chromatographie fraction from the exudates of the sea anemone Phyllactis flosculifera . Toxicon , 34 : 443 – 450 .
  • Mas , R. , Menendez , R. , Garateix , A. , Garcia , M. and Chavez , M. 1989 . Effects of a high molecular weight toxin from Physalia physalis on glutamate responses . Neuroscience 1989 , 33 : 269 – 273 .
  • Menendez , R. , Mas , R. , Garateix , A. , Garcia , M. and Chavez , M. 1990 . Effects of a high molecular weight polypeptidic toxin from Physalia physalis (Portuguese man-of-war} on cholinergic responses . Comparative Biochemistry and Physiology C. , 95 : 63 – 69 .
  • Garcia , M. E. 1997 . Isolation, purification and partial characterization of two new neurotoxins from the jelly fish Physalia physalis , Cuba : Biochemistry Department, University of Havana . Master in science thesis.(in Spanish}
  • Pazos , F. , Górnez , T. , Tejuca , M. , Alvarez , C. and Lanio , M. E. 1993 . Enzymatic characteristics of a fraction with phospholipase activity isolated from the anemone Stichodactyla helianthus . Revista Biologin , 7 : 115 – 123 . (in Spanish}
  • Castaneda , O. , Sotolongo , V. , Amor , A. M. , Stockiln , R. , Anderson , A. I. , Harvey , A. L. , Engstrom , A. , Wernstedt , C. and Karlsson , E. 1995 . Characterization of a potassium channel toxin from the Caribbean Sea anemone Stichodactyla helianthus . Toxicon , 33 : 603 – 613 .
  • Loret , E. P. , Menéndez-Soto del Valle , R. , Mensuelle , P. , Sampieri , P. and Rochat , H. 1994 . Positively charged amino acid residues located similarly in sea anemone and scorpion toxins . Journal of Biological Chemistry , 269 : 16785 – 16788 .
  • Antuen , W. , Berndt , K. D. , Chavez , M. A. , Delfin , I. and Wuthrich , K. 1993 . The NMR solution structure of a Kunitz-type proteinase inhibitor from the sea anemone Stichodactyla helianthus . European Journal of Biochemistry 15. , 212 : 675 – 684 .
  • Delfin , J. , Gonzalez , Y. , Diaz , J. and Chavez , M. 1994 . Proteinase inhibitors from Stichodactyla helianthus: purification, characterization and immobilization . Archives of Medical Research , 25 : 199 – 204 .
  • Kem , W. R. and Dunn , B. M. 1988 . Separation and characterization of four different amino acid sequence variants of a sea anemone (Stichodactyla helianthus} protein cytolysin . Toxicon , 26 : 997 – 1008 .
  • Stevens , S. M. , Kem , W. R. and Prokai , L. 2002 . Investigation of cytolysin variants by peptide mapping: enhanced protein characterization using complementary ionization and mass spectrometric techniques . Rapid Communications in Mass Spectrometry , 16 : 2094 – 2101 .
  • Huerta , V. , Morera , V. , Guanche , Y. , Chinea , G. , Gonzalez , L. I. , Betancourt , L. , Martinez , D. , Alvarez , C. , Lanio , M. E. and Besada , V. 2001 . Primary structure of two cytolysin isoforms from Stichodactyla helianthus differing in their hemolytic activity . Toxicon , 39 : 1253 – 1256 .
  • Anderluh , G. and Macek , P. 2002 . Cytolytic peptide and protein toxins from sea anemone (Anthozoa: Actinaria} . Toxicon , 40 : 111 – 124 .
  • Anderluh , G. , Pungercar , J. , Krizaj , I. , Strukelj , B. , Gubensek , F. and Macek , P. 1997 . Nterminal truncation mutagenesis of equinatoxin II, a pore-forming protein from the sea anemone Actinia equina . Protein Engineering , 10 : 751 – 755 .
  • Menestrina , G. , Cabiaux , V. and Tejuca , M. 1999 . Secondary structure of sea anemone cytolysins in soluble and membrane bound form by infrared spectroscopy . Biochemical and Biophysical Research Communications , 254 : 174 – 180 .
  • Martinez , D. , Campos , A. M. , Pazos , F. , Alvarez , C. , Lanio , M. E. , Schreer , S. , Salinas , R. K. , Vergara , C. and Lisa , E. 2001 . Properties St-I and St-II, two isotoxins isolated from Stichodactyla helianthus . A comparison. Toxicon , 39 : 1547 – 1560 .
  • Pazos , I. F. , Alvarez , C. , Rodeiro , I. , Tejuca , M. and Lanio , M. E. 1996 . Purification of a phospholipase A2 from sea anemone Stichodactyla helianthus . Revista Biologia , 10 : 115 – 123 . (in Spanish}
  • Goudet , C. , Ferrer , T. , Glan , L. , Artiles , A. , Batiste , C. F. V. , Possani , L. D. , Alvarez , I. , Aneiros , A. and Tytgat , J. 2001 . Characterization of two Bunodosoma granulifera toxins active on cardiac sodium channels . British Journal of Pharmacology , 134 : 1195 – 1206 .
  • Norton , R. S. 1991 . Structure and structure-function relationships of sea anemone proteins that interact with the sodium channel . Toxicon , 29 : 1051 – 1084 .
  • Could , A. R. , Mabbutt , B. C. and Norton , R. S. 1990 . Structure-function relationships in the polypeptide cardiac stimulant, anthopleurin-A . Effects of limited proteolysis by trypsin. European Journal of Biochemistry , 189 : 145 – 153 .
  • Kern , W. R. 1988 . “ Sea anemone toxins: structure and action ” . In Biology of the Nematocysts , Edited by: Hessinger , D. A. and Lenhoff , H. M. 375 – 85 . New York : Academic Press .
  • Bernheimer , A. W. 1990 . “ Cytolytic peptides of sea anemones ” . In Marine Toxins: Origin, Structure and Molecular Pharmacology , Edited by: Hall , S. and Strichartz , G. 304 – 311 . Washington, D. C. : American Chemical Society .
  • Macek , P. , Belmonte , G. , Pederzolli , C. and Menestrina , G. 1994 . Mechanism of action of equinatoxin II a cytolysin from the sea anemone Actinia equina L. belonging to the family of actinoporins . Toxicology , 87 : 205 – 227 .
  • Tejuca , M. , Alvarez , C. , Lanio , M. E. and Pazos , L F. 1994 . Effects of different factors on the hemolytic activity of a cytolysin from Stichodactyla helianthus . Revista Biologia , 8 : 39 – 46 . (in Spanish}
  • Tejuca , M. , Dalla-Serra , M. , Ferreras , M , Lanio , M. E. and Menestrina , G. 1996 . The mechanism of membrane permeabilisation by sticholysin I, a cytolysin isolated from the venom of the sea anemone Stichodactyla helianthus . Biochemistry , 35 : 14947 – 14957 .
  • Doyle , J. W. and Kern , W. R. 1989 . Binding of a radiolabeled sea anemone cytolysin to erythrocyte membranes . Biochimica et Biophysica Acta , 987 : 181 – 186 .
  • Malovrh , P. , Barlic , A. , Podlesek , Z. , Menestrina , G. , Macek , P. and Anderluh , G. 2000 . Structure/function studies of thryptophan mutants of equinatoxin II a sea anemone poreforming protein . Biochemical Journal , 346 : 223 – 232 .
  • Macek , P. , Zecchini , M. , Pederzolli , C. , Dalla-Serra , M. and Menestrina , G. 1995 . Intrinsic tryptophan fluorescence of equinatoxin II a pore-forming polypeptide from the sea anemone Actinia equina L., monitors its interaction with lipid membranes . European Journal of Biochemistry , 234 : 329 – 335 .
  • Martin-Benito , J. , Gavilanes , F. , De Los Rios , V. , Mancheno , J. M. , Fernandez , J. I. and Gavilanes , I. G. 2000 . Two-dimensional crystallization on lipid monolayers and threedimensional structure of sticholysin II a cytolysin from the sea anemone Stichodactyla helianthus . Biophysical Journal , 78 : 3186 – 3194 .
  • MacGregor , D. R. D. and Tobias , C. A. 1972 . Molecular sieving of red cell membranes during gradual osmotic hemolysis . Journal of Membrane Biology , 10 : 345 – 356 .
  • Renkin , E. M. 1954 . Filtration, diffusion, and molecular sieving through porous cellulose membranes . Journal of General Physiology , 38 : 225 – 243 .
  • Ginsburg , H. and Stein , W. D. 1987 . Biophysical analysis of novel transport pathways induced in red blood cell membranes . Journal of Membrane Biology , 96 : 1 – 10 .
  • Tejuca , M. , Dalla-Serra , M. , Potrich , C. , Alvarez , C and Menstrina , G. 2001 . Sizing the radius of the pore formed in erythrocytes and lipid vesicles by the toxin sticholysin I from the sea anemone Stichodactyla helianthus . Journal of Membrane Biology , 183 : 125 – 135 .
  • Lindemann , B. 1982 . Dependence of ion flow through channels on the density of fixed charges at the channel opening; Voltage control of inverse titration curve . Biophysical Journal , 39 : 15 – 22 .
  • Ropele , M. and Menestrina , G. 1989 . Electrical properties and molecular architecture of the channel formed by K coli hemolysin in planar lipid membranes . Biochimica et Biophysica Acta , 985 : 9 – 18 .
  • Trias , J. and Benz , R. 1993 . Characterization of the channel formed by the mycobacterial porin in lipid bilayer membranes. Demonstration of voltage gating and of negative point charges at the channel mouth . Journal of Biological Chemistry. , 268 : 6234 – 6240 .
  • Anderluh , G. , Krisaj , I. , Strukelj , B. , Gubensek , P. , Macek , P. and Pungercar , J. 1999 . Equinatoxins, pore-forming proteins from the sea anemone Actinia equina, belong to a multigene family . Toxicon , 37 : 1391 – 1401 .
  • Athanasiadis , A. , Anderluh , G. , Macek , P. and Tutk , D. 2001 . Crystal structure of the soluble form of equinatoxin II a pore-forming toxin from sea anemone Actinia equina . Structure , 9 : 341 – 346 .
  • Hinds , M. G. , Zhang , W. , Anderluh , G. , Hansen , P. E. and Norton , R. S. 2002 . Solution structure of the eukaryotic pore-forming cytolysin equinatoxin II implications for pore formation . Journal of Molecular Biology , 315 : 1219 – 1229 .
  • Alvarez , C. , Lanio , M. E. , Tejuca , M. , Martinez , D. , Pazos , I. F. , Campos , A. M. , Encinas , M. V. , Pertinhez , T. , Schreier , S. and Lissi , E. 1998 . The role of ionic strength on the enhancement of the hemolytic activity of sticholysin I, a cytolysin from Stichodactyla helianthus . Toxicon , 36 : 165 – 178 .
  • Marinez , D. , Soto , C. , Casallanovo , P. , Pazos , P. , Alvarez , C. , Campos , A. M. , Schreier , S. , Lanio , M. E. and Lissi , E. 2002 . Influence of membrane phase state on the association of sticholysin II a pore forming protein form Stichodactyla helianthus . Revista Biologia , 16 : 8593 (in Spanish}
  • Alvarez , C. , Dalla-Serra , M. , Potrich , C. , Bernhart , I. , Tejuca , M. , Martinez , D. , Pazos , I. P. , Lanio , M. E. and Menestrina , G. 2001 . Effects of lipid composition on membrane permeabilization by Sticholysin I and II two cytolysins of the sea anemone Stichodactyla helianthus . Biophysical Journal , 80 : 2761 – 2774 .
  • Meinardi , E. , Florin-Christensen , M , Paratcha , G. , Azcurra , J. M. and Florin-Christensen , J. 1995 . The molecular basis of the self/non self-selectivity of a coelenterate toxin . Biochemical and Biophysical Research Communications , 216 : 348 – 354 .
  • Catterall , W. A. 2000 . From ionic currents to molecular mechanisms: the structure and function of voltage gated sodium channels . Neuron , 26 : 13 – 25 .
  • Pennington , M. W. , Byrnes , M. E. , Zaydenberg , I. , Khaytin , I. , De Chastoray , J. , Krafte , D. S. , Hill , R. , Mahnir , V. M. , Volberg , W. A. and Kern , W. R. 1995 . Chemical synthesis and characterization of ShK toxin: a potent potassium channel inhibitor from a sea anemone . International Journal of Peptides and Protein Research , 46 : 354 – 358 .
  • Garateix , A. , Vega , R. , Salceda , E. , Cebada , J. , Aneiros , A. and Soto , E. 2000 . BgK anemone toxin inhibits outward K+ currents in snail neurons . Brain Research , 864 : 312 – 314 .
  • Cotton , J. , Crest , M. , Bouet , P. , Alessandri , N. , GoIa , M. , Forest , E. , Karlsson , E. , Castaneda , O. , Harvey , A. L. , Vita , C. and Menez , A. 1997 . A potassium channel toxin from the sea anemone Bunodosoma granullfera an inhibitor for Kv 1 channels. Revision of the amino acid sequence, disulfide-bridge assigment, chemical synthesis and biological activity . European Journal of Biochemistry , 244 : 192 – 202 .
  • Kem , W. R. , Pennington , M. W. and Norton , R. 1999 . Sea anemone toxins as templates for the design of immunosuppressant drugs . Perspectives in Drug Discovery and Design , 15/16 : 111 – 129 .
  • Alessandri-Haber , N. , Lecoq , A. , Gasparini , S. , Grangier-Macmath , G. , Jacquet , G. , Harvey , A. L. , de Medeiros , C , Rowan , E. G. , GoIa , M. , Menez , A. and Crest , M. 1999 . Mapping the functional anatomy of BgK on Kv 1.1, Kv 1.2 and Kv 1.3 . Journal of Biological Chemistry , 274 : 35653 – 35661 .
  • Kaiman , K. , Pennington , M. W. , Lanigan , M. D. , Nguyen , A. , Rauer , H. , Mahnir , V. , Paschetto , K. , Kem , W. R. , Grissmer , G. A. , Christina , E. P. , Cahalan , M. D. , Norton , R. S. and Chandy , K. G. 1998 . ShK-Dap (22} a potent Kvl.3-specific immunosuppressive polypeptide . Journal of Biological Chemistry , 273 : 32697 – 32707 .
  • Rauer , H. , Pennington , M. , Cahalan , M. and Chyy , G. 1999 . Structural conservation of the pores of calcium-activated and voltage gated potassium channels determined by a sea anemone toxins . Journal of Biological Chemistry , 274 : 21885 – 21892 .
  • Tudor , J. E. , Pallaghy , P. K. , Pennington , M. W. and Norton , R. 1996 . Solution structure of ShK toxin, a novel potassium channel inhibitor from a sea anemone . Nature Structural Biology , 3 : 317 – 320 .
  • Salceda , E. , Garateix , A. and Soto , E. 2002 . The sea anemone toxins BgII and BgIII prolong the inactivation time course of the tetrodotoxin-sensitive sodium current in rat dorsal root ganglion neurons . Journal of Pharmacology and Experimental Therapeutics , 303 : 1067 – 1074 .
  • Bosman , F. , Aneiros , A. and Tytgat , J. 2002 . The sea anemone Bunodosoma granulifera contains surprisingly efficacious and potent insect-selective toxins . FEBS Letters , 532 : 131 – 134 .
  • Alvarez , C. , Pazos , F.I. , Lanio , M.E. , Martinez , D. , Schreier , S. , Casallanovo , F. , Campos , A. M. and Lissi , E. 2001 . Effects of pH on the conformation, interaction with membranes and hemolytic activity of Sticholysin II, a pore forming cytolysin from the sea anemone Stichodactyla helianthus . Toxicon , 39 : 539 – 553 .
  • Garcia , T. , Valdés , A. , Palmero , A. , Garriga , Y. , Tejuca , M. , Pazos , F. and Garateix , A. in press . Pharmacological effects of Sticholysins I and II, two cytolysins isolated from the anemone Stichodactyla helianthus . Avicennia ,
  • Diaz , A. , Brito , A. , Tejuca , M. , Alvarez , C. , Lanio , M. E. , Almagro , D. and Estrada del Cueto , Ma. 1999 . Platelet pro-coagulant effects induced by StI and StII, two cytolysins purified from the sea anemone Stichodactyla helianthus . Revista Cubana de Hematologia e Inmunologia , 15 : 182 – 189 . (in Spanish}
  • Díaz , A. , Tejuca , M. , Alvarez , C. , Lanio , M. E. and Gomez , T. 1992 . Anticoagulant effect of a total extract and some fractions isolated from the anemone Stichodactyla helianthus . Revista Iberoamericana de Trombosis y Hemostasia , 5 : 8 – 11 . (in Spanish}
  • Garateix , A. , Hernandez , J. L. , Mas , R. , Romero , L. and Chavez , M. 1990 . Effects of intra and extracellularly applied Condylactis gigantea phospholipase A on ionic currents of isolated molluscan neurons . Comparative Biochemistry and Physiology , 97 : 481 – 486 .
  • Garateix , A. , Alcorta , L. F. , Menendez , R. , Mas , R. , Romero , L. and Chavez , M. 1988 . Anticholinergic effects of toxic fractions isolated from the sea anemone Condylactis gigantea . Revista Biologia , 11 : 37 – 46 . (in Spanish}
  • Menéndez , R. , Mas , R. , García , M. E. , Garateix , A. , Sotolongo , V. and Chavez , M. 1989 . Anticholinergic effects of a high molecular weight polypeptide from Physalia physalis . Comparative Biochemistry and Physiology. , 95C : 63 – 69 .
  • Campos , A. M. , Lissi , E. A. , Vergara , C. , Lanio , M. E. , Alvarez , C. , Pazos , I. , Morera , V. , García , Y. and Martínez , D. 1999 . Kinetics and mechanism of St I modification by peroxyl radicals . Journal of Protein Chemistry , 18 : 297 – 306 .
  • Pazos , I. F. , Alvarez , C. , Lanio , M. E. , Martínez , D. , Morera , V. , Lissi , E. A. and Campos , A. M. 1998 . Modification of Sticholysin II hemolytic activity by free radicals . Toxicon , 36 : 1383 – 1393 .
  • Anderluh , G. , Pungercar , J. , Strukelj , B. , Macek , P. and Gubensek , F. 1996 . Cloning, sequencing and expression of equinatoxin II . Biochemical and Biophysical Research Communications , 220 : 437 – 442 .
  • Pungercar , I. , Anderluh , G. , Macek , P. , Gubensek , F. and Strukeli , B. 1997 . Sequence analysis of the cDNA encoding the precursor of Equinatoxin V, a newly discovered hemolysin from sea anemone Actinia equina . Biochimica et Biophysica Acta , 1341 : 105 – 107 .
  • De los Ríos , V. , Onaderra , M. , Martínez-Ruis , A. , Lacadena , J. , Mancheno , I. M. , del Pozo , A. M. and Gavilanes , J. G. 2000 . Overproduction in Escherichia coli and purification of the hemolytic protein sticholysin II from the sea anemone Stichodactyla helianthus . Protein Expression and Purification , 18 : 71 – 76 .
  • Wang , Y. , Chua , K. L. and Khoo , H. E. 2000 . A new cytolysin from the sea anemone Heteractis magnified: isolation, cDNA cloning and functional expression . Biochimica et BiophysicaActa , 1478 : 9 – 18 .
  • Tejuca , M. , Alvarez , C. , Lanio , M. E. and Menestrina , G. 1999 . Antiparasite activity of seaanemone cytolysins on Giardia duodenalis and specific targeting with anti-Giardia antibodies . International Journal for Parasitology. , 29 : 489 – 498 .
  • Avila , A. D. , de Acosta , M. C. and Lage , A. 1989 . A carcinoembryonic antigen-directed immunotoxin built by linking a monoclonal antibody to a hemolytic toxin . International Journal of Cancer , 43 : 926 – 929 .
  • Avila , A. D. , Mateo de Acosta , C. and Lage , A. 1988 . A new immunotoxin built by linking a hemolytic toxin to a monoclonal antibody specific for immature T-lymphocytics . International Journal of Cancer , 142 : 568 – 571 .

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