Enhancement of Recombinant Subtilisin YaB Production by Bacillus subtilis

REFERENCES

• Bolhuis , A. , Tjalsma , H. , Smith , H.E. , de Jong , A. , Meima , R. , Venema , G. , Bron , S. and van Dijl , J.M. 1999 . Evaluation of bottlenecks in the late stages of protein secretion in Bacillus subtilis . Appl. Environ. Microbiol. , 65 ( 7 ) : 2934 – 2941 .
   PubMed Web of Science ®Google Scholar
• Braaz , R. , Wong , S.L. and Jendrossek , D. 2002 . Production of PHA depolymerase A (PhaZ5) from Paucimonas lemoignei in Bacillus subtilis . FEMS Microbiol. Lett. , 209 : 237 – 241 .
   Google Scholar
• Britton , R.A. , Echienberger , P. , Gonzalez-Pastor , J.E. , Fawcett , P. , Moson , V. , Losick , R. and Grossman , A.D. 2002 . Genome-wide analysis of the stationary-phase σ factor (σ-H) regulon of Bacillus subtilis . J. Bacteriol. , 184 ( 17 ) : 4881 – 4890 .
   PubMed Web of Science ®Google Scholar
• Gaidenko , T.A. and Price , C.W. 1998 . General stress transcription factor σB and sporulation transcription factorσH each contribute to survival of Bacillus subtilis under extreme growth conditions . J. Bacteriol. , 180 ( 14 ) : 3730 – 3733 .
   PubMed Web of Science ®Google Scholar
• Green , C.J. , Stewart , G.C. , Hollis , M.A. , Vold , B.S. and Bott , K.F. 1985 . Nucleotide sequence of the Bacillus subtilis ribosomal RNA operon, rrnB . Gene. , 37 ( 1–3 ) : 261 – 266 .
   Google Scholar
• Harwood , C.R. 1992 . Bacillus subtilis and its relatives: molecular biological and industrial workhorses . TIBTECH , 10 : 247 – 256 .
   PubMed Web of Science ®Google Scholar
• Helmann , J.D. 1995 . Compilation and analysis of Bacillus subtilis sigma A-dependent promoter sequences: evidence for extended contact between RNA polymerase and upstream promoter DNA . Nucleic Acids Res. , 23 : 2351 – 2360 .
   PubMed Web of Science ®Google Scholar
• Jensen , P.R. and Hammer , K. 1998 . Artificial promoters for metabolic optimization . Biotechnol. Bioeng. , 58 : 191 – 195 .
   PubMed Web of Science ®Google Scholar
• Jensen , P.R. and Hammer , K. 1998 . The sequence of spacers between the consensus sequences modulates the strength of prokaryotic promoters . Appl. Environ. Microbiol. , 64 : 82 – 87 .
   PubMed Web of Science ®Google Scholar
• Kaltwasser , M. , Wiegert , T. and Schumann , W. 2002 . Construction and application of epitope- and green fluorescent protein-tagging integration vectors for Bacillus subtilis . Appl. Environ. Microbiol. , 68 : 2624 – 2628 .
   PubMedGoogle Scholar
• Kaneko , R. , Koyama , N. , Tsai , Y.C. , Juang , R.Y. , Yoda , K. and Yamasaki , M. 1989 . Molecular cloning of the structural gene for alkaline elastase Ya-B, a new subtilisin produced by an alkalophilic Bacillus strain . J. Bacteriol. , 171 : 5232 – 5236 .
   Google Scholar
• Kang , I.S. , Wang , J.J. , Shih , J.C.H. and Lanier , T.C. 2004 . Extracellular production of a functional soy cystatin by Bacillus subtilis . J. Agric. Food Chem. , 52 : 5052 – 5056 .
   Google Scholar
• Kawamura , F. and Doi , R.H. 1984 . Construction of a Bacillus subtilis double mutant deficient in extracellular alkaline and neutral protease . J Bacteriol. , 160 : 442 – 444 .
   PubMed Web of Science ®Google Scholar
• Knight , C.A. , Hallett , J. and de Vries , A. L. 1988 . Solute effects on ice recrystallization: an assessment technique . Cryobiol. , 25 : 55 – 60 .
   PubMed Web of Science ®Google Scholar
• Koichiro , M. , Chen , C.L. , Akihiko , K. , Yutaka , T. , Doi , R.H. and Wong , S.L. 2002 . Heterologous production of Clostridium cellulovorans engB, using protease-deficient Bacillus subtilis and preparation of active recombinant cellulosomes . J. Bacteriol. , 184 : 76 – 81 .
   Google Scholar
• LeGrice , S.F. , Shih , C.C. , Whipple , F. and Sonenshein , A.L. 1986 . Separation and analysis of the RNA polymerase binding sites of a complex Bacillus subtilis promoter . Mol. Gen. Genet. , 204 ( 2 ) : 229 – 236 .
   PubMedGoogle Scholar
• Leighton , T.J. and Doi , R.H. 1971 . The stability of messenger RNA during sporulation in B. subtilis . J. Biol. Chem. , 246 : 3189 – 3195 .
   PubMedGoogle Scholar
• Lemmli , U.K. 1970 . Cleavages of structured proteins during the assembly of the head of bacteriophage T4 . Nature , 277 : 680 – 685 .
   Google Scholar
• Mei , H.C. , Liaw , Y.C. , Li , Y.C. , Wang , D.C. and Takagi , H. and Tsai Y.C. 1998 . Engineering subtilisin YaB: restriction of substrate specificity by the substitution of Gly124 and Gly151 with Ala . Prot. Engineer. , 11 ( 2 ) : 109 – 117 .
   PubMedGoogle Scholar
• Moriya , S. , Ogasawara , N. and Yoshikawa , H. 1985 . Structure and function of the region of the replication origin of the Bacillus subtilis chromosome. III. Nucleotide sequence of some 10,000 base pairs in the origin region . Nucleic Acids Res. , 13 ( 7 ) : 2251 – 2265 .
   PubMed Web of Science ®Google Scholar
• Nielsen , J. 2001 . Metabolic engineering . Appl. Microbiol. Biotechnol. , 55 : 263 – 283 .
   PubMed Web of Science ®Google Scholar
• Peterson , A. , Bernhardt , J. , Gerth , U. , Hoper , D. , Koburger , T. , Völker , U. and Hecker , M. 1999 . Identification of σB-dependent genes in Bacillus subtilis using a promoter consensus-directed search and oligonucleotide hybridization . J. Bacteriol. , 181 ( 18 ) : 5718 – 5724 .
   PubMed Web of Science ®Google Scholar
• Power , S.D. , Adams , R.M. and Wells , J.A. 1986 . Secretion and autoproteolytic maturation of subtilisin . Proc. Natl. Acad. Sci. USA , 83 : 3096 – 3100 .
   PubMed Web of Science ®Google Scholar
• Rollenhagen , C. , Antelmann , H. , Kirstein , J. , Delumeau , O. , Hecker , M. and Yudkin , M.D. 2003 . Binding of σA and σB to core RNA polymerase after environmental stress in Bacillus subtilis . J. Bacteriol. , 185 ( 1 ) : 35 – 40 .
   PubMed Web of Science ®Google Scholar
• Sambrook , J. and Russell , D.W. 2001 . Molecular cloning: A laboratory manual , Cold Spring Harbor, NY : Cold Spring Harbor Press, Cold Spring Harbor Laboratory .
   Google Scholar
• Schägger , H. and von Jagow , G.I. 1987 . Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa . Anal. Biochem. , 166 : 368 – 379 .
   PubMed Web of Science ®Google Scholar
• Stewart , G.C. and Bott , K.F. 1983 . DNA sequence of the tandem ribosomal RNA promoter for B. subtilis operon rrnB . Nucleic Acids Res. , 11 ( 18 ) : 6289 – 6300 .
   PubMed Web of Science ®Google Scholar
• Strauch , M.A. , Aronson , A.I. , Brown , S.W. , Schreier , H.J. and Sonenhein , A.L. 1988 . Sequence of the Bacillus subtilis glutamine synthetase gene region . Gene. , 71 ( 2 ) : 257 – 265 .
   PubMed Web of Science ®Google Scholar
• Tjalsma , H. , Bolhuis , A. , Jongbloed , H. , Bron , S. and van Dul , J.M. 2000 . Signal peptide dependent protein transport in Bacillus subtilis: a genome-based survey of the secretome, Microbiol . Mol. Biol. Rev. , 64 ( 3 ) : 515 – 547 .
   PubMed Web of Science ®Google Scholar
• Trumble , W.R. , Sherf , B.A. , Reasoner , J.L. , Seward , P.D. , Denovan , B.A. , Douthart , R.J. and West , J.W. 1992 . Protein expression from an Escherichia coli/Bacillus subtilis multifunctional shuttle plasmid with synthetic promoter sequences . Protein. Expr. Purif. , 3 : 169 – 177 .
   Google Scholar
• Tsai , Y.C. , Yamasaki , M. , Yamamoto-Suzuki , Y. and Tamura , G. 1983 . A new alkaline elastase of an alkalophilic Bacillus . Biochem. Int. , 7 : 577 – 583 .
   Google Scholar
• Wong , S.L. 1995 . Advances in the use of Bacillus subtilis for the expression and secretion of heterologous proteins . Curr. Opion. Biotechnol. , 6 : 517 – 522 .
   PubMed Web of Science ®Google Scholar
• Wong , J.P. , Yeh , C.M. and Tsai , Y.C. 2006 . Improved production of recombinant subtilisin YaB by engineering the expression control sequences in Bacillus subtilis . J. Agric. Food. Chem. , 54 : 9405 – 9410 .
   Google Scholar
• Yeh , C.M. , Chang , H.K. , Hsieh , H.M. , Yoda , K. , Yamasaki , M. and Tsai , Y.C. 1997 . Improved translational efficiency of subtilisin YaB gene with different initiation codons in Bacillus subtilis and alkalophilic Bacillus YaB . J. Appl. Microbiol. , 83 : 758 – 763 .
   Google Scholar
• Yeh , C.M. , Yang , M.C. and Tsai , Y.C. 2002 . Application potency of engineered G159 mutants on P1 substrate pocket of subtilisin YaB as improved meat tenderizers . J. Agric. Food. Chem. , 50 : 6199 – 6204 .
   PubMed Web of Science ®Google Scholar
• Yeh , C.M. , Wang , J.P. and Su , F.S. 2005 . Improved electro-transformation protocol for Bacillus subtilis DB104 . Taiwanese J. Agri. Chem and Food Sci. , 43 ( 5 ) : 325 – 333 .
   Google Scholar