Advanced search
Publication Cover
Microbial Ecology in Health and Disease Volume 14, 2002 - Issue 1
Journal homepage
421
Views
11
CrossRef citations to date
0
Altmetric
Research Article

Molecular Analysis of Intestinal Microbiota Composition to Evaluate the Effect of PEG and Lactulose Laxatives in Humans

Irène Mangin Institut National de la Recherche Agronomique, Unite dEcologie et de Physiologie du Syste me Digestif, Domaine de Vilvert, 78 352 Jouy-en-Josas, France
,
Yoram Bouhnik Hopital Lariboisiere, Service dHepato-Gastro-Enterologie et dAssistance Nutritive, Service de Bacteriologie-Virologie, 2 rue Ambroise Pare, 75 475 Paris, Cedex 10, France
,
Antonia Suau
,
Violaine Rochet
,
Laurent Raskine
,
Pascal Crenn
,
Franc¸ois Dyard Solvay-Pharma, 42 rue Rouget de Lisle, 92 151 Suresnes, France
,
Jean-Claude Rambaud
&
Joël Doré
 show all
Pages 54-62 | Published online: 11 Jul 2009

  • Moore WE, Holdeman LV. Human fecal flora: the normal flora of 20 Japanese-Hawaiians. Appl Microbiol 1974; 27: 961-79.
  • Finegold SM, Sutler VL, Mathisen GE. Normal indigenous flora. In: Hentges DJ, ed. Human Intestinal Microflora in Health and Disease. New York: Academic Press, 1983: 3-31.
  • Gibson GR, Roberfroid MB. Dietary modulation of the human colonie microbiota: introducing the concept of prebiotics. J Nutr 1995; 125: 1401-12.
  • Roberfroid MB, Van Loo JA, Gibson GR. The bifidogenic nature of chicory inulin and its hydrolysis products. J Nutr 1998; 128: 11-9.
  • Kleessen B, Sykura B, Zunft HJ, Blaut M. Effects of inulin and lactose on fecal microflora, microbial activity, and bowel habit in elderly constipated persons. Am J Clin Nutr 1997; 65: 1397-402.
  • Hidaka H, Eida T, Takizawa T, Tokunaga T, Tashiro Y. Effects of fructooligosaccharides on intestinal flora and human health. Bifidobacteria Microflora 1986; 5: 37-50.
  • Fuller R. Probiotics in medicine. Gut 1991; 32: 439-42.
  • Langendijk PS, Schut F, Jansen GJ, Raangs GC, Kamphuis GR, Wilkinson MH, et al. Quantitative fluorescence in situ hybridization of Bifidobacterium spp. with genus-specific 16S rRNA-targeted probes and its application in fecal samples. Appl Environ Microbiol 1995; 61: 3069-75.
  • Borneman J, Skroch PW, O'Sullivan KM, Palus JA, Rumjanek NG, Jansen JL, et al. Molecular microbial diversity of an agricultural soil in Wisconsin. Appl Environ Microbiol 1996; 62: 1935-43.
  • Godon JJ, Zumstein E, Dabert P, Habouzit F, Moletta R. Molecular microbial diversity of an anaerobic digester as determined by small-subunit rDNA sequence analysis. Appl Environ Microbiol 1997; 63: 2802-13.
  • Kroes I, Lepp PW, Relman DA. Bacterial diversity within the human subgingival crevice. Proc Natl Acad Sci USA 1999; 96: 14547-52.
  • Suau A, Bonnet R, Sutren M, Godon JJ, Gibson GR, Collins MD, et al. Direct analysis of genes encoding 16S rRNA from complex communities reveals many novel molecular species within the human gut. Appl Environ Microbiol 1999; 65: 4799-807.
  • Wilson KH, Blitchington RB. Human colonie biota studied by ribosomal DNA sequence analysis. Appl Environ Microbiol 1996; 62: 2273-8.
  • Zoetendal EG, Akkermans AD, De Vos WM. Temperature gradient gel electrophoresis analysis of 16S rRNA from human fecal samples reveals stable and host-specific communities of active bacteria. Appl Environ Microbiol 1998; 64: 3854-9.
  • Doré J, Sghir A, Hannequart-Gramet G, Corthier G, Pochart P. Design and evaluation of a 16S rRNA-targeted oligonucleotide probe for specific detection and quantitation of human faecal Bacteroides populations. Syst Appl Microbiol 1998; 21: 65-71.
  • Sghir A, Gramet G, Suau A, Rochet V, Pochait P, Doré J. Quantification of bacterial groups within human fecal flora by oligonucleotide probe hybridization. Appl Environ Microbiol 2000; 66: 2263-6.
  • Salminen S, Salminen E. Lactulose, lactic acid bacteria, intestinal microecology and mucosal protection. Scand J Gastroenterol 1997; 222 (Suppl): 45-8.
  • Attar A, Lemann M, Ferguson A, Halphen M, Boutron MC, Flourié B, et al. Comparison of a low dose polyethylene glycol electrolyte solution with lactulose for treatment of chronic constipation. Gut 1999; 44: 226-30.
  • Sghir A, Antonopoulos D, Mackie RI. Design and evaluation of a Lactobacillus group-specific ribosomal RNAtargeted hybridization probe and its application to the study of intestinal microecology in pigs. Syst Appl Microbiol 1998; 21: 291-6.
  • Franks AH, Harmsen HJ, Raangs GC, Jansen GJ, Schut F, Welling GW. Variations of bacterial populations in human feces measured by fluorescent in situ hybridization with group-specific 16S rRNA-targeted oligonucleotide probes. Appl Environ Microbiol 1998; 64: 3336-45.
  • Amann RI, Krumholz L, Stahl DA. Fluorescent-oligonucleotide probing of whole cells for determinative, phylogenetic, and environmental studies in microbiology. J Bacteriol 1990; 172: 762-70.
  • Zheng D, Aim EW, Stahl DA, Raskin L. Characterization of universal small-subunit rRNA hybridization probes for quantitative molecular microbial ecology studies. Appl Environ Microbiol 1996; 62: 4504-13.
  • Aim EW, Oerther DB, Larsen N, Stahl DA, Raskin L. The oligonucleotide probe database. Appl Environ Microbiol 1996; 62: 3557-9.
  • Maidak BL, Olsen GJ, Larsen N, Overbeek R, McCaughey MJ, Woese CR. The RDP (Ribosomal Database Project). Nucleic Acids Res 1997; 25: 109-11.
  • Stahl DA, Flesher B, Mansfield HR, Montgomery L. Use of phylogenetically based hybridization probes for studies of ruminai microbial ecology. Appl Environ Microbiol 1988; 54: 1079-84.
  • Raskin L, Stromley JM, Rittmann BE, Stahl DA. Groupspecific 16S rRNA hybridization probes to describe natural communities of methanogens. Appl Environ Microbiol 1994; 60: 1232-40.
  • Ballongue J, Schumann C, Quignon P. Effects of lactulose and lactitol on colonie microflora and enzymatic activity. Scand J Gastroenterol 1997; 222 (Suppl): 41-4.
  • Doré J, Gramet G, Goderel I, Pochait P. Culture-independant characterisation of human faecal flora using rRNAtargeted hybridisation probes. Genet SeI Evol 1998; 1 (Suppl): 287S-96S.
  • Harmsen HJ, Gibson GR, Elfferich P, Raangs GC, Wildeboer-Veloo AC, Argaiz A, et al. Comparison of viable cell counts and fluorescence in situ hybridization using specific rRNA-based probes for the quantification of human fecal bacteria. FEMS Microbiol Lett 2000; 183: 125-9.
  • Kaufmann P, Pfefferkorn A, Teuber M, Meile L. Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR. Appl Environ Microbiol 1997; 63: 1268-73.
  • Scardovi V. Bergey's Manual of Systematic Bacteriology: Genus Bifidobacterium. In: Sneath PHA, Mair NS, Sharpe ME, Holt JG, eds. vol 2, Baltimore: Williams & Wilkins, 1986: 1418-34.
  • Nebra Y, Blanch AR. A new selective medium for Bifidobacterium spp. Appl Environ Microbiol 1999; 65: 5173-6.
  • Bouhnik Y, Riottot M, Telotte A, Attar A, Dyard F, Flourié B. Lactulose ingestion increases fecal bifidobacterial counts. A randomized double blind study in healthy humans. Gut 1999; 45: A288.
  • Bouhnik Y, Flourié B, Andrieux C, Bisetti N, Briet F, Rambaud JC. Effects of Bifidobacterium sp fermented milk ingested with or without inulin on colonie bifidobacteria and enzymatic activities in healthy humans. Eur J Clin Nutr 1996; 50: 269-73.
  • Bouhnik Y, Flourié B, D'Agay-Abensour L, Pochait P, Gramet G, Durand M, et al. Administration of transgalactooligosaccharides increases fecal bifidobacteria and modifies colonie fermentation metabolism in healthy humans. J Nutr 1997; 127: 444-8.
  • Bouhnik Y, Vahedi K, Achour L, Attar A, Salfati J, Pochart P, et al. Short-chain fructo-oligosaccharide administration dose-dependently increases fecal bifidobacteria in healthy humans. J Nutr 1999; 129: 113-6.
  • Gibson GR, Beatty ER, Wang X, Cummings JH. Selective stimulation of bifidobacteria in the human colon by oligofructose and inulin. Gastroenterology 199S; 108: 97582.
  • Kruse HP, Kleessen B, Blaut M. Effects of inulin on faecal bifidobacteria in human subjects. Br J Nutr 1999; 82: 37582.
  • Macfarlane GT, Cummings JH. Probiotics and prebiotics:can regulating the activities of intestinal bacteria benefit health? Br Med J 1999; 318: 999-1003.
  • Liévin V, Peiffer I, Hudault S, Rochat F, Brassart D, Neeser JR, et al. Bifidobacterium strains from resident infant human gastrointestinal microflora exert antimicrobial activity. Gut 2000; 47: 646-52.
  • Crittenden RG. Prebiotics. In: Probiotics: A Critical Review. Wymondham, UK: Horizon Scientific Press, 1999: 141-56.
  • Matsuki T, Watanabe K, Tanaka R, Fukuda M, Oyaizu H. Distribution of bifidobacterial species in human intestinal microflora examined with 16S rRNA-gene-targeted speciesspecific primers. Appl Environ Microbiol 1999; 65: 4506-12.

Related research

People also read lists articles that other readers of this article have read.

Recommended articles lists articles that we recommend and is powered by our AI driven recommendation engine.

Cited by lists all citing articles based on Crossref citations.
Articles with the Crossref icon will open in a new tab.