Heterologous extracellular DNA facilitates the development of Trichosporon asahii and T. inkin biofilms and enhances their tolerance to antifungals

References

• Alhede M, Alhede M, Qvortrup K, Kragh KN, Jensen PØ, Stewart PS, Bjarnsholt T. 2020. The origin of extracellular DNA in bacterial biofilm infections in vivo. Pathog Dis. 78:ftaa018. doi:10.1093/femspd/ftaa018
   PubMed Web of Science ®Google Scholar
• Bongomin F, Otu A, Calisti G, Richardson MD, Barnard J, Venkateswaran R, Vergidis P. 2019. Trichosporon japonicum fungemia and ventricular assist device infection in an imnunocompetent patient. Open Forum Infect Dis. 6:ofz343. doi:10.1093/ofid/ofz343
   PubMed Web of Science ®Google Scholar
• Carvalho MHGF, Santos FB, Nosanchuk JD, Zancope-Oliveira RM, Almeida-Paes R. 2014. L-dihidroxyphenylalanine induces melanin production by members of the genus Trichosporon. FEMS Yeast Res. 14:988–991. doi:10.1111/1567-1364.12174.
   PubMed Web of Science ®Google Scholar
• Collins TJ. 2007. ImageJ for microscopy. Biotechniques. 43:S25–S30. doi:10.2144/000112517
   PubMed Web of Science ®Google Scholar
• Cordeiro RA, Serpa R, Alexandre CFU, Marques FJF, Melo CVS, Franco JS, Evangelista AJJ, Camargo ZP, Brilhante RSN, Rocha MFG, et al. 2015. Trichosporon inkin biofilms produce extracellular proteases and exhibit resistance to antifungals. J Med Microbiol. 64:1277–1286. doi:10.1099/jmm.0.000159
   PubMed Web of Science ®Google Scholar
• De Hoog GS, Guarro J, Gené J, Figueras MJ. 2000. Atlas of clinical fungi. 2nd ed. Baarn (The Nederlands): Centraalbureau voor Schimmslcultures.
   Google Scholar
• Di Bonaventura G, Pompilio A, Picciani C, Iezzi M, D'Antonio D, Piccolomini R. 2006. Biofilm formation by the emerging fungal pathogen trichosporon asahii: development, architecture, and antifungal resistance. Antimicrob Agents Chemother. 50:3269–3276. doi:10.1128/AAC.00556-06
   PubMed Web of Science ®Google Scholar
• Iturrieta-Gonzalez IA, Padovan ACB, Bizerra FC, Hahn RC, Colombo AL. 2014. Multiple species of Trichosporon produce biofilms highly resistant to triazoles and amphotericin B. PLoS One. 9:e109553. doi:10.1371/journal.pone.0109553.
   PubMed Web of Science ®Google Scholar
• Ibáñez de Aldecoa AL, Zafra O, González-Pastor JE. 2017. Mechanisms and regulation of extracellular DNA release and its biological roles in microbial communities. Front Microbiol. 8:1390. doi:10.3389/fmicb.2017.01390
   PubMed Web of Science ®Google Scholar
• Jakubovics NS, Shields RC, Rajarajan N, Burgess JG. 2013. Life after death: the critical role of extracellular DNA in microbial biofilms. Lett Appl Microbiol. 57:467–475. doi:10.1111/lam.12134
   PubMed Web of Science ®Google Scholar
• Karygianni L, Ren Z, Koo H, Thurnheer T. 2020. Biofilm matrixome: extracellular components in structured microbial communities. Trends Microbiol. 28:668–681. doi:10.1016/j.tim.2020.03.016
   PubMed Web of Science ®Google Scholar
• Kurakado S, Chiba R, Sato C, Matsumoto Y, Sugita T. 2020. N,N,N′,N′-tetrakis(2-pyridylmethyl) ethylenediamine, a zinc chelator, inhibits biofilm and hyphal formation in Trichosporon asahii. BMC Res Notes. 13:6. doi:10.1186/s13104-020-04990-x
   PubMed Web of Science ®Google Scholar
• Liao S, Klein MI, Heim KP, Fan Y, Bitoun JP, Ahn S, Burne RA, Koo H, Brady LJ, Wen ZT. 2014. Streptococcus mutans Extracellular DNA is upregulated during growth in biofilms, actively released via membrane vesicles, and influenced by components of the protein secretion machinery. J Bacteriol. 196:2355–2366. doi:10.1128/JB.01493-14
   PubMed Web of Science ®Google Scholar
• Mariné M, Brown NA, Riaño-Pachón DM, Goldman GH. 2015. On and under the skin: emerging basidiomycetous yeast infections caused by Trichosporon species. PLoS Pathog. 11:e1004982–21. doi:10.1371/journal.ppat.1004982
   PubMed Web of Science ®Google Scholar
• Martinez LR, Casadevall A. 2006. Susceptibility of Cryptococcus neoformans biofilm to antifungal agents in vitro. Antimicrob Agents Chemother. 50:1021–1033. doi:10.1128/AAC.50.3.1021-1033.2006
   PubMed Web of Science ®Google Scholar
• Martins M, Henriques M, Lopez-Ribot JL, Oliveira R. 2012. Addition of DNase improves the in vitro activity of antifungal drugs against Candida albicans biofilms. Mycoses. 55:80–85. doi:10.1111/j.1439-0507.2011.02047.x
   PubMed Web of Science ®Google Scholar
• Martins M, Uppuluri P, Thomas DP, Cleary IA, Henriques M, Lopez-Ribot JL, Oliveira R. 2010. Presence of extracellular DNA in the Candida albicans biofilm matriz and its contribution to biofilms. Mycopathologia. 169:323–331. doi:10.1007/s11046-009-9264-y
   PubMed Web of Science ®Google Scholar
• Okshevsky M, Meyer RL. 2015. The role of extracellular DNA in the establishment, maintenance and perpetuation of bacterial biofilms. Crit Rev Microbiol. 41:341–352. doi:10.3109/1040841X.2013.841639
   PubMed Web of Science ®Google Scholar
• Okshevsky M, Regina VR, Meyer RL. 2015. Extracellular DNA as a target for biofilm control. Curr Opin Biotechnol. 33:73–80. doi:10.1016/j.copbio.2014.12.002.
   PubMed Web of Science ®Google Scholar
• Peeters E, Nelis HJ, Coenye T. 2008. Comparison of multiple methods for quantification of microbial biofilms grown in microtiter plates. J Microbiol Methods. 72:157–165. doi:10.1016/j.mimet.2007.11.010
   PubMed Web of Science ®Google Scholar
• Ramírez I, Moncada D. 2020. Fatal Disseminated infection by Trichosporon asahii under voriconazole therapy in a patient with acute myeloid leukemia: a review of breakthrough infections by Trichosporon spp. Mycopathologia. 185:377–388. doi:10.1007/s11046-019-00416-w
   PubMed Web of Science ®Google Scholar
• Rajendran R, Sherry L, Lappin DF, Nile CJ, Smith K, Williams C, Munro CA, Ramage G. 2014. Extracellular DNA release confers heterogeneity in Candida albicans biofilm formation. BMC Microbiol. 14:303. doi:10.1186/s12866-014-0303-6
   PubMed Web of Science ®Google Scholar
• Rajendran R, Williams C, Lappin DF, Millington O, Martins M, Ramage G. 2013. Extracellular DNA release acts as an antifungal resistance mechanism in mature Aspergillus fumigatus biofilms. Eukaryot Cell. 12:420–429. doi:10.1128/EC.00287-12
   PubMedGoogle Scholar
• Rodriguez-Tudela JL, Diaz-Guerra TM, Mellado E, Cano V, Tapia C, Perkins A, Gomez-Lopez A, Rodero L, Cuenca E. 2005. Susceptibility patterns and molecular identification of Trichosporon species. Antimicrob Agents Chemother. 49:4026–4034. doi:10.1128/AAC.49.10.4026-4034.2005
   PubMed Web of Science ®Google Scholar
• Sapaar B, Nur A, Hirota K, Yumoto H, Murakami K, Amoh T, Matsuo T, Ichikawa T, Miyake Y. 2014. Effects of extracellular DNA from Candida albicans and pneumonia-related pathogens on Candida biofilm formation and hyphal transformation. J Appl Microbiol. 116:1531–1542. doi:10.1111/jam.12483
   PubMed Web of Science ®Google Scholar
• Shopova I, Bruns S, Thywissen A, Kniemeyer O, Brakhage AA, Hillmann F. 2013. Extrinsic extracellular DNA leads to biofilm formation and colocalizes with matrix polysaccharides in the human pathogenic fungus Aspergillus fumigatus. Front Microbiol. 4:141. doi:10.3389/fmicb.2013.00141
   PubMed Web of Science ®Google Scholar
• Smolarz M, Zawrotniak M, Satala D, Rapala-Kozik M. 2021. Extracellular nucleic acids present in the candida albicans biofilm trigger the release of neutrophil extracellular traps. Front Cell Infect Microbiol. 11:681030. doi:10.3389/fcimb.2021.681030
   PubMed Web of Science ®Google Scholar
• Sugimoto S, Sato F, Miyakawa R, Chiba A, Onodera S, Hori S, Mizunoe Y. 2018. Broad impact of extracellular DNA on biofilm formation by clinically isolated Methicillin-resistant and -sensitive strains of Staphylococcus aureus. Sci Rep. 8:2254. doi:10.1038/s41598-018-20485-z
   PubMed Web of Science ®Google Scholar
• Taverna CG, Córdoba S, Murisengo OA, Vivot W, Davel G, Bosco-Borgeat ME. 2014. Molecular identification, genotyping, and antifungal susceptibility testing of clinically relevant Trichosporon species from Argentina. Med Mycol. 52:356–366. doi:10.1093/mmy/myt029
   PubMed Web of Science ®Google Scholar