659
Views
6
CrossRef citations to date
0
Altmetric
Articles

Selection and evaluation of reference genes for expression analysis of Cassi

, , , , , , , , & show all
Pages 1818-1826 | Received 11 Mar 2015, Accepted 11 May 2015, Published online: 16 Jun 2015

References

  • Heid CA, Stevens J, Livak KJ, Williams PM. Real time quantitative PCR. Genome Res. 1996;6:986–994.10.1101/gr.6.10.986
  • Huggett J, Dheda K, Bustin S, Zumla A. Real-time RT-PCR normalisation strategies and considerations. Genes Immun. 2005;6:279–284.10.1038/sj.gene.6364190
  • Andersen CL, Jensen JL, Ørntoft TF. Normalization of real-time quantitative reverse transcription-PCR data: a model-based variance estimation approach to identify genes suited for normalization, applied to bladder and colon cancer data sets. Cancer Res. 2004;64:5245–5250.10.1158/0008-5472.CAN-04-0496
  • Bustin SA, Nolan T. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 2004;15:155–166.
  • Fleige S, Pfaffl MW. RNA integrity and the effect on the real-time qRT-PCR performance. Mol. Aspects Med. 2006;27:126–139.10.1016/j.mam.2005.12.003
  • Radonić A, Thulke S, Mackay IM, Landt O, Siegert W, Nitsche A. Guideline to reference gene selection for quantitative real-time PCR. Biochem. Biophys. Res. Commun. 2004;313:856–862.10.1016/j.bbrc.2003.11.177
  • Li Q-F, Sun SS, Yuan D-Y, Yu H-X, Gu M-H, Liu Q-Q. Validation of candidate reference genes for the accurate normalization of real-time quantitative RT-PCR data in rice during seed development. Plant Mol. Biol. Rep. 2010;28:49–57.10.1007/s11105-009-0124-1
  • Chen L, Zhong H-y, Kuang J-f, Li J-g, Lu W-j, Chen J-y. Validation of reference genes for RT-qPCR studies of gene expression in banana fruit under different experimental conditions. Planta. 2011;234:377–390.10.1007/s00425-011-1410-3
  • Czechowski T, Stitt M, Altmann T, Udvardi MK, Scheible W-R. Genome-wide identification and testing of superior reference genes for transcript normalization in Arabidopsis. Plant Physiol. 2005;139:5–17.10.1104/pp.105.063743
  • Schmidt GW, Delaney SK. Stable internal reference genes for normalization of real-time RT-PCR in tobacco (Nicotiana tabacum) during development and abiotic stress. Mol. Genet. Genomics. 2010;283:233–241.10.1007/s00438-010-0511-1
  • Narsai R, Ivanova A, NgS, Whelan J. Defining reference genes in Oryza sativa using organ, development, biotic and abiotic transcriptome datasets. BMC Plant Biol. 2010;10:56–65.10.1186/1471-2229-10-56
  • Warzybok A, Migocka M. Reliable reference genes for normalization of gene expression in Cucumber Grown under different nitrogen nutrition. PLoS One. 2013;8:e72887.10.1371/journal.pone.0072887
  • Reid KE, Olsson N, Schlosser J, Peng F, Lund ST. An optimized grapevine RNA isolation procedure and statistical determination of reference genes for real-time RT-PCR during berry development. BMC Plant Biol. 2006;6:27–36.10.1186/1471-2229-6-27
  • Long X-Y, Wang J-R, Ouellet T, Rocheleau H, Wei Y-M, Pu Z-E, Jiang Q-T, Lan X-J, Zheng Y-L. Genome-wide identification and evaluation of novel internal control genes for Q-PCR based transcript normalization in wheat. Plant Mol. Biol. 2010;74:307–311.10.1007/s11103-010-9666-8
  • Hu R, Fan C, Li H, Zhang Q, Fu Y-F. Evaluation of putative reference genes for gene expression normalization in soybean by quantitative real-time RT-PCR. BMC Mol. Biol. 2009;10:93–99.10.1186/1471-2199-10-93
  • Barsalobres-Cavallari CF, Severino FE, Maluf MP, Maia IG. Identification of suitable internal control genes for expression studies in Coffea arabica under different experimental conditions. BMC Mol. Biol. 2009;10:1–10.10.1186/1471-2199-10-1
  • Kim S-J, Kim K-W, Kim D-S, Kim M-C, Jeon Y-D, Kim S-G, Jung H-J, Jang H-J, Lee B-C, Chung W-S. The protective effect of Cassia obtusifolia on DSS-induced colitis. Am. J. Chin. Med. 2011;39:565–577.10.1142/S0192415X11009032
  • Dave H, Ledwani L. A review on anthraquinones isolated from Cassia species and their applications. Indian J. Nat. Prod. Res. 2012;3:291–319.
  • Sadiq I, Shuaibu M, Bello A, Isah STA, Izuagie T, Nasiru S, Kamaru M. Phytochemistry and antimicrobial activities of Cassia occidentalis used for herbal remedies. Methods. 2012;18:19–28.
  • Singh D, Sharma S, Rani R, Mishra S, Sharma R. A new flavonoid and other two flavonoids isolated from different plant parts of selected Cassia species. Asian J. Res. Chem. 2011;4:818–821.
  • Tzeng T-F, Lu H-J, Liou S-S, Chang CJ, Liu I-M. Reduction of lipid accumulation in white adipose tissues by Cassia tora (Leguminosae) seed extract is associated with AMPK activation. Food Chem. 2013;136:1086–1094.10.1016/j.foodchem.2012.09.017
  • Dai, Y-c, Deng, N, Liu, W, Tan, B-y. Extraction, isolation and purification of aurantio-obtusin in Cassia seeds. Cent. South Pharm. 2011;3:1–7.
  • Liao H, Ren W, Kang Z, Jiang J-H, Zhao X-J, Du L-F. A trypsin inhibitor from Cassia obtusifolia seeds: isolation, characterization and activity against Pieris rapae. Biotechnol. Lett. 2007;29:653–658.10.1007/s10529-006-9281-6
  • Yang Y-C, Lim M-Y, Lee H-S. Emodin isolated from Cassia obtusifolia (Leguminosae) seed shows larvicidal activity against three mosquito species. J. Agric. Food Chem. 2003;51:7629–7631.10.1021/jf034727t
  • Vandesompele J, De Preter K, Pattyn F, Poppe B, Van Roy N, De Paepe A, Speleman F. Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes. Genome Biol. 2002;3:1–34.
  • Liu Z, Song T, Zhu Q, Wang W, Zhou J, Liao H. De novo assembly and analysis of Cassia obtusifolia seed transcriptome to identify genes involved in the biosynthesis of active metabolites. Biosci. Biotechnol. Biochem. 2014;78:791–799.
  • Schlotter YM, Veenhof EZ, Brinkhof B, Rutten VP, Spee B, Willemse T, Penning LC. A GeNorm algorithm-based selection of reference genes for quantitative real-time PCR in skin biopsies of healthy dogs and dogs with atopic dermatitis. Vet. Immunol. Immunopathol. 2009;129:115–118.10.1016/j.vetimm.2008.12.004
  • Wang Q, Ishikawa T, Michiue T, Zhu B-L, Guan D-W, Maeda H. Stability of endogenous reference genes in postmortem human brains for normalization of quantitative real-time PCR data: comprehensive evaluation using geNorm, NormFinder, and BestKeeper. Int. J. Legal Med. 2012;126:943–952.10.1007/s00414-012-0774-7
  • Pfaffl MW, Tichopad A, Prgomet C, Neuvians TP. Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper—Excel-based tool using pair-wise correlations. Biotech. Lett. 2004;26:509–515.10.1023/B:BILE.0000019559.84305.47
  • Al-Menaie H, Al-Ragam O, Al-Shatti A, Mathew M, Suresh N. Interactive effect of soil mixture and fertilizer concentration on the growth of Cassia seedlings. Eur. J. Sci. Res. 2013;94:61–66.
  • Rushton PJ, Somssich IE, Ringler P, Shen QJ. WRKY transcription factors. Trends Plant Sci. 2010;15:247–258.10.1016/j.tplants.2010.02.006
  • Chen L, Song Y, Li S, Zhang L, Zou C, Yu D. The role of WRKY transcription factors in plant abiotic stresses. Biochim. Biophys. Acta (BBA)—Gene Regul. Mech. 2012;1819:120–128.10.1016/j.bbagrm.2011.09.002

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.