The tyrosine-sorting motif of the vacuolar sorting receptor VSR4 from Arabidopsis thaliana, which is involved in the interaction between VSR4 and AP1M2, μ1-adaptin type 2 of clathrin adaptor complex 1 subunits, participates in the post-Golgi sorting of VSR4Footnote

Abbreviations: Amp^r, ampicillin resistance marker; AP, adaptor protein; AP1M, μ1-adaptin of AP1 complex; AP1M2, AP1M type 2; BiFC, bimolecular fluorescence complementation; BP, a recombination between an att B-flanking DNA fragment and an att P-containing donor vector; CaMV, cauliflower mosaic virus; CDS, coding sequence; CCV, clathrin-coated vesicle; CLSM, confocal laser scanning microscope; COPI, coat protein I; COPII, coat protein II; CRS, cargo-recognition sequence; cV, C-terminal region (211–239) of Venus; ECFP, enhanced cyan fluorescent protein; EE, early endosome; ER, endoplasmic reticulum; GFP, green fluorescent protein; IP, immunoprecipitation; Km^r, kanamycin resistance marker; mCherry, monomeric Cherry; nV, N-terminal region (1–210) of Venus; ORF, open reading frame; P_35S, 35S promoter; LR, a recombination between an att L-containing entry clone and an att R-containing destination vector; PCR, polymerase chain reaction; PM, plasma membrane; PPI, protein–protein interaction; PVDF, polyvinylidene fluoride; RFP, red fluorescent protein; RME, receptor-mediated endocytosis; SD, standard deviation; SDS-PAGE, SDS‐polyacrylamide gel electrophoresis; sGFP, synthetic GFP with S65T; SYP, syntaxin of plants; tdTomato, tandem dimer Tomato; TGN, trans-Golgi network; VM, vacuolar membrane; VSR, vacuolar-sorting receptor; YFP, yellow fluorescent protein.

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