1,170
Views
10
CrossRef citations to date
0
Altmetric
Regular Papers

Substantial decrease in cell wall α-1,3-glucan caused by disruption of the kexB gene encoding a subtilisin-like processing protease in Aspergillus oryzae

, , , , , , , & show all
Pages 1781-1791 | Received 23 Dec 2015, Accepted 18 Feb 2016, Published online: 15 Mar 2016

References

  • Fuller RS, Brake A, Thorner J. Yeast prohormone processing enzyme (KEX2 gene product) is a Ca2+-dependent serine protease. Proc. Nat. Acad. Sci. U.S.A. 1989;86:1434–1438.10.1073/pnas.86.5.1434
  • Mizuno K, Nakamura T, Ohshima T, et al. Yeast KEX2 gene encodes an endopeptidase homologous to subtilisin-like serine proteases. Biochem. Biophys. Res. Commun. 1988;156:246–254.10.1016/S0006-291X(88)80832-5
  • Fuller RS, Brake AJ, Thorner J. Intracellular targeting and structural conservation of a prohormone-processing endoprotease. Science. 1989;246:482–486.10.1126/science.2683070
  • Gómez-Saladín E, Wilson DL, Dickerson IM. Isolation and in situ localization of a cDNA encoding a Kex2-like prohormone convertase in the nematode Caenorhabditis elegans. Cell. Mol. Neurobiol. 1994;14:9–25.10.1007/BF02088586
  • Nakayama K, Hosaka M, Hatsuzawa K, et al. Cloning and functional expression of a novel endoprotease involved in prohormone processing at dibasic sites. J. Biochem. 1991;109:803–806.
  • Shennan KI, Smeekens SP, Steiner DF, et al. Characterization of PC2, a mammalian Kex2 homologue, following expression of the cDNA in microinjected Xenopus oocytes. FEBS Lett. 1991;284:277–280.10.1016/0014-5793(91)80703-6
  • Bader O, Schaller M, Klein S, et al. The KEX2 gene of Candida glabrata is required for cell surface integrity. Mol. Microbiol. 2001;41:1431–1444.10.1046/j.1365-2958.2001.02614.x
  • Newport G, Kuo A, Flattery A, et al. Inactivation of Kex2p diminishes the virulence of Candida albicans. J. Biol. Chem. 2003;278:1713–1720.10.1074/jbc.M209713200
  • Jalving R, van de Vondervoort PJ, Visser J, et al. Characterization of the kexin-like maturase of Aspergillus niger. Appl. Environ. Microbiol. 2000;66:363–368.10.1128/AEM.66.1.363-368.2000
  • Punt PJ, Drint-Kuijvenhoven A, Lokman BC, et al. The role of the Aspergillus niger furin-type protease gene in processing of fungal proproteins and fusion proteins. J. Biotechnol. 2003;106:23–32.10.1016/j.jbiotec.2003.09.005
  • Mizutani O, Nojima A, Yamamoto M, et al. Disordered cell integrity signaling caused by disruption of the kexB gene in Aspergillus oryzae. Eukaryot. Cell. 2004;3:1036–1048.10.1128/EC.3.4.1036-1048.2004
  • Fujioka T, Mizutani O, Furukawa K, et al. MpkA-dependent and -independent cell wall integrity signaling in Aspergillus nidulans. Eukaryot. Cell. 2007;6:1497–1510.10.1128/EC.00281-06
  • Futagami T, Seto K, Kajiwara Y, et al. The putative stress sensor protein MtlA is required for conidia formation, cell wall stress tolerance, and cell wall integrity in Aspergillus nidulans. Biosci. Biotechnol. Biochem. 2014;78:326–335.10.1080/09168451.2014.878218
  • Katayama T, Ohta A, Horiuchi H. Protein kinase C regulates the expression of cell wall-related genes in RlmA-dependent and independent manners in Aspergillus nidulans. Biosci. Biotechnol. Biochem. 2015;79:321–330.10.1080/09168451.2014.973365
  • Smits GJ, Kapteyn JC, van den Ende H, et al. Cell wall dynamics in yeast. Curr. Opin. Microbiol. 1999;2:348–352.10.1016/S1369-5274(99)80061-7
  • Fontaine T, Simenel C, Dubreucq G, et al. Molecular organization of the alkali-insoluble fraction of Aspergillus fumigatus cell wall. J. Biol. Chem. 2000;275:27594–27607.
  • Beauvais A, Latgé JP. Membrane and cell wall targets in Aspergillus fumigatus. Drug Resist. Update. 2001;4:38–49.10.1054/drup.2001.0185
  • Bernard M, Latgé JP. Aspergillus fumigatus cell wall: composition and biosynthesis. Med. Mycol. 2001;39:9–17.10.1080/744118873
  • Akao T, Sano M, Yamada O, et al. Analysis of expressed sequence tags from the fungus Aspergillus oryzae cultured under different conditions. DNA Res. 2007;14:47–57.10.1093/dnares/dsm008
  • Machida M, Asai K, Sano M, et al. Genome sequencing and analysis of Aspergillus oryzae. Nature. 2005;438:1157–1161.10.1038/nature04300
  • Kobayashi T, Abe K, Asai K, et al. Genomics of Aspergillus oryzae. Biosci. Biotechnol. Biochem. 2007;71:646–670.10.1271/bbb.60550
  • Reissig JL, Storminger JL, Leloir LF. A modified colorimetric method for the estimation of N-acetylamino sugars. J. Biol. Chem. 1955;217:959–966.
  • Dubois M, Gilles K, Hamilton JK, et al. A colorimetric method for the determination of sugars. Nature. 1951;168:167.10.1038/168167a0
  • Dubois M, Gilles K, Hamilton JK, et al. Colorimetric method for determination of sugars and related substances. Anal. Chem. 1956;28:350–356.10.1021/ac60111a017
  • Cabib E, Kang MS, Au-Young J. Chitin synthase from Saccharomyces cerevisiae. Methods Enzymol. 1987;138:643–649.10.1016/0076-6879(87)38058-9
  • Latgé JP. The cell wall: a carbohydrate armour for the fungal cell. Mol. Microbiol. 2007;66:279–290.10.1111/mmi.2007.66.issue-2
  • Schaffner W, Weissmann C. A rapid, sensitive, and specific method for the determination of protein in dilute solution. Anal. Biochem. 1973;56:502–514.10.1016/0003-2697(73)90217-0
  • Kelly R, Register E, Hsu MJ, et al. Isolation of a gene involved in 1,3-beta-glucan synthesis in Aspergillus nidulans and purification of the corresponding protein. J. Bacteriol. 1996;178:4381–4391.
  • Shida M, Ushioda Y, Nakajima T, et al. Structure of the alkali-insoluble skeletal glucan of Lentinus edodes. J. Biochem. 1981;90:1093–1100.
  • Hakomori S. A rapid permethylation of glycolipid, and polysaccharide catalyzed by methylsulfinyl carbanion in dimethyl sulfoxide. J. Biochem. 1964;55:205–208.
  • Watanabe T, Kobori K, Miyashita K, et al. Identification of glutamic acid 204 and aspartic acid 200 in chitinase A1 of Bacillus circulans WL-12 as essential residues for chitinase activity. J. Biol. Chem. 1993;268:18567–18572.
  • Nelson N. A photometric adaptation of the somogyi method for the determination of the glucose. J. Biol. Chem. 1944;153:375–380.
  • Somogyi M. Notes on sugar determination. J. Biol. Chem. 1952;195:19–22.
  • Manners DJ, Masson AJ, Sturgeon RJ. An enzymic method for the determination of the degree of polymerisation of glucans. Carbohydr. Res. 1971;17:109–114.10.1016/S0008-6215(00)81547-5
  • Sugawara T, Takahashi S, Osumi M, et al. Refinement of the structures of cell-wall glucans of Schizosaccharomyces pombe by chemical modification and NMR spectroscopy. Carbohydr. Res. 2004;339:2255–2265.10.1016/j.carres.2004.05.033
  • Yano S, Wakayama M, Tachiki T. Cloning and expression of an α-1,3-glucanase gene from Bacillus circulans KA-304: the enzyme participates in protoplast formation of Schizophyllum commune. Biosci. Biotechnol. Biochem. 2006;70:1754–1763.10.1271/bbb.60095
  • Rappleye CA, Eissenberg LG, Goldman WE. Histoplasma capsulatum alpha-(1,3)-glucan blocks innate immune recognition by the beta-glucan receptor. Proc. Nat. Acad. Sci. 2007;104:1366–1370.10.1073/pnas.0609848104
  • Fujikawa T, Kuga Y, Yano S, et al. Dynamics of cell wall components of Magnaporthe grisea during infectious structure development. Mol. Microbiol. 2009;73:553–570.10.1111/mmi.2009.73.issue-4
  • Fontaine T, Beauvais A, Loussert C, et al. Cell wall α1-3glucans induce the aggregation of germinating conidia of Aspergillus fumigatus. Fungal Genet. Biol. 2010;47:707–712.10.1016/j.fgb.2010.04.006
  • Yoshimi A, Sano M, Inaba A, et al. Functional analysis of the α-1,3-glucan synthase genes agsA and agsB in Aspergillus nidulans: agsB is the major α-1,3-glucan synthase in this fungus. PLoS One. 2013;8:e54893.10.1371/journal.pone.0054893
  • Damveld RA, Arentshorst M, Franken A, et al. The Aspergillus niger MADS-box transcription factor RlmA is required for cell wall reinforcement in response to cell wall stress. Mol. Microbiol. 2005;58:305–319.10.1111/j.1365-2958.2005.04827.x
  • Damveld RA, vanKuyk PA, Arentshorst M, et al. Expression of agsA, one of five 1,3-α-d-glucan synthase-encoding genes in Aspergillus niger, is induced in response to cell wall stress. Fungal Genet. Biol. 2005;42:165–177.10.1016/j.fgb.2004.11.006
  • Henry C, Latge JP, Beauvais A. α1,3 Glucans are dispensable in Aspergillus fumigatus. Eukaryot. Cell. 2012;11:26–29.10.1128/EC.05270-11
  • Perez P, Rincón SA. Rho GTPases: regulation of cell polarity and growth in yeasts. Biochem. J. 2010;426:243–253.10.1042/BJ20091823
  • Dichtl K, Helmschrott C, Dirr F, et al. Deciphering cell wall integrity signalling in Aspergillus fumigatus: identification and functional characterization of cell wall stress sensors and relevant Rho GTPases. Mol. Microbiol. 2012;83:506–519.10.1111/mmi.2012.83.issue-3
  • Kwon MJ, Arentshorst M, Roos ED, et al. Functional characterization of Rho GTPases in Aspergillus niger uncovers conserved and diverged roles of Rho proteins within filamentous fungi. Mol. Microbiol. 2011;79:1151–1167.10.1111/mmi.2011.79.issue-5
  • Mizutani O, Furukawa K, Ichiyanagi S, et al. Alternative processing of proproteins in Aspergilli kexB gene disruptants under hyperosmotic conditions. Biosci. Biotechnol. Biochem. 2009;73:40–46.10.1271/bbb.80437
  • Mouyna I, Hartland RP, Fontaine T, et al. A 1,3-beta-glucanosyltransferase isolated from the cell wall of Aspergillus fumigatus is a homologue of the yeast Bgl2p. Microbiology. 1998;144:3171–3180.10.1099/00221287-144-11-3171
  • Carotti C, Ragni E, Palomares O, et al. Characterization of recombinant forms of the yeast Gas1 protein and identification of residues essential for glucanosyltransferase activity and folding. Eur. J. Biochem. 2004;271:3635–3645.10.1111/ejb.2004.271.issue-18
  • Hartland RP, Fontaine T, Debeaupuis JP, et al. A novel beta-(1-3)-glucanosyltransferase from the cell wall of Aspergillus fumigatus. J. Biol. Chem. 1996;271:26843–26849.
  • Mouyna I, Morelle W, Vai M, et al. Deletion of GEL2 encoding for a β(1-3)glucanosyltransferase affects morphogenesis and virulence in Aspergillus fumigatus. Mol. Microbiol. 2005;56:1675–1688.10.1111/j.1365-2958.2005.04654.x
  • Cid VJ, Duran A, del Rey F, et al. Molecular basis of cell integrity and morphogenesis in Saccharomyces cerevisiae. Microbiol. Rev. 1995;59:345–386.
  • Rodriguez-Pena JM, Cid VJ, Arroyo J, et al. A novel family of cell wall-related proteins regulated differently during the yeast life cycle. Mol. Cell Biol. 2000;20:3245–3255.10.1128/MCB.20.9.3245-3255.2000
  • Smits GJ, van den Ende H, Klis FM. Differential regulation of cell wall biogenesis during growth and development in yeast. Microbiology. 2001;147:781–794.10.1099/00221287-147-4-781
  • Larriba G, Basco RD, Andaluz E, et al. Yeast exoglucanases. Where redundancy implies necessity. Arch. Med. Res. 1993;24:293–299.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.