599
Views
3
CrossRef citations to date
0
Altmetric
Note

The glycosylation stoichiometry of EWS species in neuronal cells

&
Pages 165-167 | Received 23 May 2016, Accepted 13 Aug 2016, Published online: 08 Sep 2016

References

  • Schwartz JC, Cech TR, Parker RR. Biochemical properties and biological functions of FET proteins. Annu. Rev. Biochem. 2015;84:3.1–3.25.
  • Li H, Watford W, Li C, et al. Ewing sarcoma gene EWS is essential for meiosis and B lymphocyte development. J. Clin. Invest. 2007;117:1314–1323.10.1172/JCI31222
  • Cho J, Shen H, Yu H, et al. Ewing sarcoma gene Ews regulates hematopoietic stem cell senescence. Blood. 2011;117:1156–1166.10.1182/blood-2010-04-279349
  • Park JH, Kang HJ, Kang SI, et al. A multifunctional protein, EWS, is essential for early brown fat lineage determination. Dev. Cell. 2013;26:393–404.10.1016/j.devcel.2013.07.002
  • Park JH, Lee SB. An essential role for Ewing sarcoma gene (EWS) in early white adipogenesis. Obesity. 2015;23:138–144.10.1002/oby.20934
  • Azuma M, Embree LJ, Sabaawy H, et al. Ewing sarcoma protein Ewsr1 maintains mitotic integrity and proneural cell survival in the zebrafish embryo. PLoS One. 2007;2:e979.10.1371/journal.pone.0000979
  • Dormann D, Haass C. Fused in sarcoma (FUS): an oncogene goes awry in neurodegeneration. Mol. Cell. Neurosci. 2013;56:475–486.10.1016/j.mcn.2013.03.006
  • Matsuoka Y, Matsuoka Y, Shibata S, et al. Identification of Ewing's sarcoma gene product as a glycoprotein using a monoclonal antibody that recognizes an immunodeterminant containing O-linked N-acetylglucosamine moiety. Hybrid. Hybrydomics. 2002;21:233–236.10.1089/153685902760213831
  • Wells L, Vosseller K, Cole RN, et al. Mapping sites of O-GlcNAc modification using affinity tags for serine and threonine post-translational modifications. Mol. Cell. Proteomics. 2002;1:791–804.10.1074/mcp.M200048-MCP200
  • Ishihara K, Takahashi I, Tsuchiya Y, et al. Characteristic increase in nucleocytoplasmic protein glycosylation by O-GlcNAc in 3T3-L1 adipocyte differentiation. Biochem. Biophys. Res. Commun. 2010;398:489–494.10.1016/j.bbrc.2010.06.105
  • Li Q, Kamemura K. Adipogenesis stimulates the nuclear localization of EWS with an increase in its O-GlcNAc glycosylation in 3T3-L1 cells. Biochem. Biophys. Res. Commun. 2014;450:588–592.10.1016/j.bbrc.2014.06.013
  • Kishi M, Mizuseki K, Sasai N, et al. Requirement of Sox2-mediated signaling for differentiation of early Xenopus neuroectoderm. Development. 2000;127:791–800.
  • Przyborski SA, Morton IE, Wood A, et al. Developmental regulation of neurogenesis in the pluripotent human embryonal carcinoma cell line NTERA-2. Eur. J. Neurosci. 2000;12:3521–3528.10.1046/j.1460-9568.2000.00230.x
  • Lee MK, Tuttle JB, Rebhun L, et al. The expression and posttranslational modification of a neuron-specific β-tubulin isotype during chick embryogenesis. Cell Motil. Cytoskeleton. 1990;17:118–132.10.1002/(ISSN)1097-0169
  • Rexach JE, Rogers CJ, Yu S-H, et al. Quantification of O-glycosylation stoichiometry and dynamics using resolvable mass tags. Nat. Chem. Biol. 2010;6:645–651.10.1038/nchembio.412
  • Nagel AK, Ball LE. O-GlcNAc modification of the runt-related transcription factor 2 (Runx2) links osteogenesis and nutrient metabolism in bone marrow mesenchymal stem cells. Mol. Cell. Proteomics. 2014;13:3381–3395.10.1074/mcp.M114.040691
  • Bachmaier R, Aryee DNT, Jug G, et al. O-GlcNAcylation is involved in the transcriptional activity of EWS-FLI1 in Ewing’s sarcoma. Oncogene. 2009;28:1280–1284.10.1038/onc.2008.484
  • Gupta R, Brunak S. Prediction of glycosylation across the human proteome and the correlation to protein function. Pac. Symp. Biocomput. 2002;7:310–322.
  • Livak KJ, Schmittgen TD. Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods. 2001;25:402–408.10.1006/meth.2001.1262

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.