Purification and characterization of a novel alginate lyase from the marine bacterium Cobetia sp. NAP1 isolated from brown algaeFootnote

Abbreviations: CFE, cell free extract; ESI-MS, electrospray ionization mass spectrometry; HPLC, high-performance liquid chromatography; MALDI-TOF-MS, matrix-assisted laser desorption/ionization-time of flight-mass spectrometry; polyG, polyguluronic acid-rich region; polyM, polymannuronic acid-rich region; PL, polysaccharide lyase; polyMG, random region; SEC, size exclusion chromatography; SDS-PAGE, sodium dodecyl sulfate-polyacrylamide gel electrophoresis.

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