1,028
Views
9
CrossRef citations to date
0
Altmetric
Biochemistry & Molecular Biology

Expression and characterization of recombinant human lactoferrin in edible alga Chlamydomonas reinhardtii

, , , , , & show all
Pages 851-859 | Received 16 Nov 2018, Accepted 07 Jan 2019, Published online: 23 Jan 2019

References

  • Mayeur S, Spahis S, Pouliot Y, et al. Lactoferrin, a pleiotropic protein in health and disease. Antioxid Redox Signal. 2016;24(14):813–836.
  • Garcia-Montoya IA, Cendon TS, Arevalo-Gallegos S, et al. Lactoferrin a multiple bioactive protein: an overview. Biochim Biophys Acta. 2012;1820(3):226–236.
  • Sharma S, Sinha M, Kaushik S, et al. C-lobe of lactoferrin: the whole story of the half-molecule. Biochem Res Int. 2013;2013:271641.
  • Expósito LM, Montes RL, Rodríguez LM, et al. Multifunctional capacity and therapeutic potential of lactoferrin. Life Sci. 2018;195:61–64.
  • Wang X, Wang X, Hao Y, et al. Research and development on lactoferrin and its derivatives in China from 2011–2015. Biochem Cell Biol. 2017;95(1):162–170.
  • Legrand D. Overview of lactoferrin as a natural immune modulator. J Pediatr. 2016;173(Suppl):S10–S15.
  • Nandi S, Suzuki YA, Huang J, et al. Expression of human lactoferrin in transgenic rice grains for the application in infant formula. Plant Sci. 2002;163(4):713–722.
  • Pang XN, Hong X, Wei X, et al. Research progress in physicochemical characteristics of lactoferrin and its recombinant expression systems. Hereditas. 2015;37(9):873–884. [Article in Chinese].
  • Almond RJ, Flanagan BF, Antonopoulos A, et al. Differential immunogenicity and allergenicity of native and recombinant human lactoferrins: role of glycosylation. Eur J Immunol. 2013;43(1):170–181.
  • Scaife MA, Nguyen GT, Rico J, et al. Establishing Chlamydomonas reinhardtii as an industrial biotechnology host. Plant J. 2015;82(3):532–546.
  • Mayfield SP, Manuell AL, Chen S, et al. Chlamydomonas reinhardtii chloroplasts as protein factories. Curr Opin Biotechnol. 2007;18(2):126–133.
  • Rochaix JD. The three genomes of Chlamydomonas. Photosynth Res. 2002;73(1–3):285–293.
  • Specht E, Miyake-Stoner S, Mayfield S. Micro-algae come of age as a platform for recombinant protein production. Biotechnol Lett. 2010;32(10):1373–1383.
  • Almaraz-Delgado AL, Flores-Uribe J, Pérez-España VH, et al. Production of therapeutic proteins in the chloroplast of Chlamydomonas reinhardtii. AMB Express. 2014;4:57.
  • Yoon SM, Kim SY, Li KF, et al. Transgenic microalgae expressing Escherichia coli AppA phytase as feed additive to reduce phytate excretion in the manure of young broiler chicks. Appl Microbiol Biotechnol. 2011;91:553–563.
  • Yagi T, Yamashita K, Okada N, et al. Hydrogen photoproduction in green algae Chlamydomonas reinhardtii sustainable over 2 weeks with the original cell culture without supply of fresh cells nor exchange of the whole culture medium. J Plant Res. 2016;129:771–779.
  • Gong W, Zhao L, Hu B, et al. Identifying novel salt-tolerant genes from Dunaliella salina using a Haematococcus pluvialis expression system. Plant Cell Tissue Org Cult. 2014;117:113–124.
  • Wang H, Chen X, Hao X, et al. High throughput screening atrazine chlorohydrolase mutants with enhanced activity through Haematococcus pluvialis expression system. Sheng Wu Gong Cheng Xue Bao. 2011;27(4): 620–628. [Article in Chinese].
  • Díaz-Santos E, Vega M, Vila M, et al. Efficiency of different heterologous promoters in the unicellular microalga Chlamydomonas reinhardtii. Biotechnol Prog. 2013;29(2):319–328.
  • Zhang R, Patena W, Armbruster U, et al. High-throughput genotyping of green algal mutants reveals random distribution of mutagenic insertion sites and endonucleolytic cleavage of transforming DNA. Plant Cell. 2014;26(4):1398–1409.
  • Mirimin L, Roodt-Wilding R. Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus. J Fish Biol. 2015;86(3):1218–1223.
  • Chang S, Chen W, Yang J. Another formula for calculating the gene change rate in real-time RT-PCR. Mol Biol Rep. 2009;36(8):2165–2168.
  • Campos-Quevedo N, Rosales-Mendoza S, Paz-Maldonado LMT, et al. Production of milk-derived bioactive peptides as precursor chimeric proteins in chloroplasts of Chlamydomonas reinhardtii. Plant Cell Tissue Org Cult. 2013;113(2):217–225.
  • Jones CG, Hare JD, Compton SJ. Measuring plant protein with the Bradford assay. J Chem Ecol. 1989;15:979–992.
  • Li Q, Hong X, Chen XW, et al. Expression and characterization of recombinant cellulases from an isolated Klebsiella. Afr J Microbiol Res. 2015;9(38):2057–2066.
  • Dekanski D, Spremo-Potparević B, Bajić V, et al. Acute toxicity study in mice of orally administrated TiO2 nanoparticles functionalized with caffeic acid. Food Chem Toxicol. 2018;115:42–48.
  • Wang JY, Fang M, Boye A, et al. Interaction of microRNA-21/145 and Smad3 domain-specific phosphorylation in hepatocellular carcinoma. Oncotarget. 2017 May 9;8(49):84958–84973.
  • Kim SN, Lee JH, Shin H, et al. Effects of in vitro-digested ginsenosides on lipid accumulation in 3T3-L1 adipocytes. Planta Med. 2009;75:596–601.
  • EFSA. Panel on dietetic products, nutrition and allergies (NDA). Scientific opinion on bovine lactoferrin. EFSA J. 2012;10(7):2811.
  • Rosales-Mendoza S, Paz-Maldonado LMT, Soria-Guerra RE. Chlamydomonas reinhardtii as a viable platform for the production of recombinant proteins: current status and perspectives. Plant Cell Rep. 2012;31:479–494.
  • Shao N, Bock R. A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii. Curr Genet. 2008;53:381–388.
  • Barahimipour R, Neupert J, Bock R. Efficient expression of nuclear transgenes in the green alga Chlamydomonas: synthesis of an HIV antigen and development of a new selectable marker. Plant Mol Biol. 2016;90(4–5):03–18.
  • Eichler-Stahlberg A, Weisheit W, Ruecker O, et al. Strategies to facilitate transgene expression in Chlamydomonas reinhardtii. Planta. 2009;229(4):873–883.
  • Dauvillée D, Delhaye S, Gruyer S, et al. Engineering the chloroplast targeted malarial vaccine antigens in Chlamydomonas starch granules. PLoS One. 2010;5(12):e15424.
  • Rasala BA, Lee PA, Shen Z, et al. Robust expression and secretion of Xylanase1 in Chlamydomonas reinhardtii by fusion to a selection gene and processing with the FMDV 2A peptide. PLoS One. 2012;7(8):e43349.
  • Cerven D, DeGeorge G, Bethell D. 28-Day repeated dose oral toxicity of recombinant human apo-lactoferrin or recombinant human lysozyme in rats. Regul Toxicol Pharmacol. 2008;51(2):162–167.
  • Appel MJ, van Veen HA, Vietsch H, et al. Sub-chronic (13-week) oral toxicity study in rats with recombinant human lactoferrin produced in the milk of transgenic cows. Food Chem Toxicol. 2006;44(7):964–973.
  • Neupert J, Shao N, Lu Y, et al. Genetic transformation of the model green alga Chlamydomonas reinhardtii. Methods Mol Biol. 2012;847:35–47.
  • Barahimipour R, Strenkert D, Neupert J, et al. Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardti. Plant J. 2015;84:704–717.
  • Lauersen KJ, Berger H, Mussgnug JH, et al. Efficient recombinant protein production and secretion from nuclear transgenes in Chlamydomonas reinhardtii. J Biotechnol. 2013;167:101–110.

Reprints and Corporate Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

To request a reprint or corporate permissions for this article, please click on the relevant link below:

Academic Permissions

Please note: Selecting permissions does not provide access to the full text of the article, please see our help page How do I view content?

Obtain permissions instantly via Rightslink by clicking on the button below:

If you are unable to obtain permissions via Rightslink, please complete and submit this Permissions form. For more information, please visit our Permissions help page.