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Short Communication

Inhibition of agonist-induced platelet aggregation by magnesium sulfate warrants its use as an alternative in vitro anticoagulant in pseudothrombocytopenia

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Pages 680-684 | Received 26 Jan 2019, Accepted 08 Aug 2019, Published online: 11 Sep 2019

References

  • England JM, Rowan RM, Van Assendelft OW, Bull BS, Coulter W, Fujimoto K, Groner W, Richardson-Jones A, Klee G, Koepke JA, Lewis SM, McLaren CE, Shinton NK, Tatsumi N, Verwilghen RL. Recommendations of the international council for standardization in haematology for ethylenediaminetetraacetic acid anticoagulation of blood for blood cell counting and sizing. Am J Clin Pathol. 1993;100(4):371–372. doi:10.1093/ajcp/100.6.618
  • Pegels JG, Bruynes EC, Engelfriet CP, von dem Borne AEGKr. Pseudothrombocytopenia: An immunologic study on platelet antibodies dependent on ethylene diamine tetra-acetate. Blood. 1982;59(1):157–161.
  • Gowland E, Kay HEM, Spillman JC, Williamson JR. Agglutination of platelets by a serum factor in the presence of EDTA. J Clin Pathol. 1969;22(4):460–464. [Accessed 31 May 2016]. doi:10.1136/jcp.22.4.460
  • Schrezenmeier H, Muller H, Gunsilius E, Heimpel H, Seifried. Anticoagulant-induced pseudothrombocytopenia and pseudoleucocytosis. Thromb Haemost. 1995;73(3):506–513.
  • Lombarts AJ, De Kieviet W. Recognition and prevention of pseudothrombocytopenia and concomitant pseudoleukocytosis. Am J Clin Pathol. 1988;89(5):634–639. doi:10.1093/ajcp/89.5.634
  • Rosenkranz B, Wisser H, Bode JC. Pseudothrombopenie–eine Storung der Thrombozytenzahlbestimmung. Deutsche medizinische Wochenschrift. 1985;110(13):492–495. doi:10.1055/s-2008-1068851
  • Bizzozero J. Ueber einen neuen Formbestandtheil des Blutes und dessen Rolle bei der Thrombose und der Blutgerinnung - Untersuchungen. Archiv für Pathologische Anatomie und Physiologie und für Klinische Medicin. 1882;90(2):261–332.
  • Ravn HB, Kristensen SD, Vissinger H, Husted SE. Magnesium inhibits human platelets. Blood Coagul Fibrin. 1996;7(2):241–244. doi:10.1097/00001721-199603000-00033
  • Shechter M, Merz CN, Paul-Labrador M, Sheehan CJ, Zagrodzky JD, Abraham TP, Page RL, Hamdan MH. Oral magnesium supplementation inhibits platelet-dependent thrombosis in patients with coronary artery disease. Am J Cardiol. 1999;84(2):152–156. doi:10.1016/s0002-9149(99)00348-3
  • Gawaz M. Antithrombotic effects of magnesium. Possible therapeutic consequences for acute coronary syndromes | Antithrombozytare Wirksamkeit von Magnesium. Mogliche Konsequenzen fur die akut ischamische Herzerkrankung. Fortschr Med. 1996;114(26):47–50.
  • Golański J, Pietrucha T, Baj Z, Montes R, Borbolla JR, Montaño LF, Rocha E. A novel approach to inhibit the anticoagulant-induced spontaneous activation of blood platelets - Effect of magnesium on platelet release reaction in whole blood. Thromb Res. 1997;85(2):127–132. doi:10.1016/s0049-3848(97)00036-4
  • Nakamoto K, Sugibayashi S, Takahashi A, Terauchi S, Hada A, Munakata M, Teraoka A, Komiyama Y, Egawa H, Murata K. Platelet count in EDTA-dependent pseudothrombocytopenia–application of MgSO4 as an anticoagulant. Rinsho Byori. 1986;34(2):167–173.
  • Schuff-Werner P, Steiner M, Fenger S, Gross H-J, Bierlich A, Dreissiger K, Mannuß S, Siegert G, Bachem M, Kohlschein P. Effective estimation of correct platelet counts in pseudothrombocytopenia using an alternative anticoagulant based on magnesium salt. Br J Haematol. 2013;162(5):684–692. doi:10.1111/bjh.2013.162.issue-5
  • Mannuß S, Kohlschein P, Dreißiger K, Schuff-Werner P. Measurement of Platelet Counts and Volume Using Magnesium Sulfate as an Anticoagulant: Comparison of Impedance and Light-Scatter Technology. Am J Clin Pathol. 2016;146(5):538–545. doi:10.1093/ajcp/aqw146
  • François D, Masure A, Atallah N, Touil L, Vasse M. Underestimation of platelet count on magnesium salt-anticoagulated samples. Clin Chem Lab Med. 2014;52(5):e95–e97. doi:10.1515/cclm-2013-1016
  • Toth O, Calatzis A, Penz S, Losonczy H, Siess W. Multiple electrode aggregometry: A new device to measure platelet aggregation in whole blood. Thromb Haemost. 2006;96(6):781–788. doi:10.1160/TH06-05-0242
  • Yardumian DA, Mackie IJ, Machin SJ. Laboratory investigation of platelet function: A review of methodology. J Clin Pathol. 1986;39(7):701–712. doi:10.1136/jcp.39.7.701
  • Sanner BM, Konermann M, Tepel M, Groetz J, Mummenhoff C, Zidek W. Platelet function in patients with obstructive sleep apnoea syndrome. Eur Respir J. 2000;16(4):648–652. doi:10.1034/j.1399-3003.2000.16d14.x
  • Kalb ML, Potura L, Scharbert G, Kozek-Langenecker SA. The effect of ex vivo anticoagulants on whole blood platelet aggregation. Platelets. 2009;20(1):7–11. doi:10.1080/09537100802364076
  • Mann KG, Whelihan MF, Butenas S, ORFEO T. Citrate anticoagulation and the dynamics of thrombin generation. J Thromb Haemost. 2007;5(10):2055–2061. Accessed 2016 May 30. doi:10.1111/jth.2007.5.issue-10
  • Stone SR, Hofsteenge J. Kinetics of the inhibition of thrombin by hirudin. Biochemistry. 1986;25(16):4622–4628. [Accessed 2016 May 24]. doi:10.1021/bi00364a025
  • Stalker TJ, Newman DK, Ma P, Wannemacher KM, Brass LF. Platelet signaling. In: Gresele P, Born G, Patrono C, Page C. (eds) Antiplatelet Agents. Handbook of Experimental Pharmacology. 2012. Springer, Berlin, Heidelberg. 210: 59–85.
  • Gawaz M, Ott I, Reininger AJ, Neumann FJ. Effects of magnesium on platelet aggregation and adhesion. Magnesium modulates surface expression of glycoproteins on platelets in vitro and ex vivo. Thromb haemost. 1994;72(6):912–918.
  • Murray R, FitzGerald GA. Regulation of thromboxane receptor activation in human platelets. Proc Natl Acad Sci. 1989;86(1):124–128. Available: http://www.pnas.org/content/pnas/86/1/124.full.pdf. [Accessed 2018 Sep 5].
  • FitzGerald GA. Mechanisms of platelet activation: thromboxane A2 as an amplifying signal for other agonists. Am J Cardiol. 1991;68(7):11B–15B. doi:10.1016/0002-9149(91)90379-y
  • Ghoshal K, Bhattacharyya M. Overview of platelet physiology: its hemostatic and nonhemostatic role in disease pathogenesis. ScientificWorldJournal. 2014. doi:10.1155/2014/781857. Accessed 2016 Jun 1.
  • Hwang DL, Yen CF, Nadler JL. Effect of extracellular magnesium on platelet activation and intracellular calcium mobilization. Am J Hypertens. 1992;5(10):700–706. doi:10.1093/ajh/5.10.700
  • Roberts DE, McNicol A, Bose R. Mechanism of Collagen Activation in Human Platelets. J Biol Chem. 2004;279(19):19421–19430. doi:10.1074/jbc.M308864200
  • Murugappa S, Kunapuli SP. The role of ADP receptors in platelet function. Front Biosci. 2006;11:1977–1986. doi:10.2741/1939
  • Grand RJA, Turnell AS, Grabham PW. Cellular consequences of thrombin-receptor activation. Biochem J. 1996;313(2):353–368. doi:10.1042/bj3130353
  • Du X. Signaling and regulation of the platelet glycoprotein Ib-IX-V complex. Curr Opin Hematol. 2007;14(3):262–269. doi:10.1097/MOH.0b013e3280dce51a
  • Volpe P, Alderson-Lang BH, Nickols GA. Regulation of inositol 1,4,5-trisphosphate-induced Ca2+ release. I. Effect of Mg2+. Am J Physiol Cell Physiol. 1990;258(6):C1077–85.
  • Hsiao G, Shen MY, Chou DS, Lin CH, Chen TF, Sheu JR. Involvement of the antiplatelet activity of magnesium sulfate in suppression of protein kinase C and the Na+/H+exchanger. J Biomed Sci. 2004;11(1):19–26.
  • Sheu JR, Hsiao G, Shen MY, Fong T-H, Chen Y-W, Lin C-H, Chou D-S. Mechanisms involved in the antiplatelet activity of magnesium in human platelets. Br J Haematol. 2002;119(4):1033–1041. doi:10.1046/j.1365-2141.2002.03967.x
  • Anfossi G, Trovati M, Malfertheiner P. Role of catecholamines in platelet function: Pathophysiological and clinical significance. Eur J Clin Invest. 1996;26(5):353–370. doi:10.1046/j.1365-2362.1996.470598.x
  • Stegner D, Nieswandt B. Platelet receptor signaling in thrombus formation. J Mol Med. 2011;89(2):109–121. doi:10.1007/s00109-010-0691-5
  • Figures WR, Scearce LM, Wachtfogel Y, Chen J, Colman RF, Colman RW. Platelet ADP receptor and alpha 2-adrenoreceptor interaction. Evidence for an ADP requirement for epinephrine-induced platelet activation and an influence of epinephrine on ADP binding. J Biol Chem. 1986;261(13):5981–5986.
  • Hardy E, Glenn J, Heptinstall S, Rubin P, Horn E. Magnesium modifies the responses of platelets to inhibitory agents which act via cAMP. Thromb Haemost. 1995;74(4):1132–1137. doi:10.1055/s-0038-1649893
  • Kovacs F, Varga M, Pataki Z, Rigo E. Pseudothrombocytopenia with multiple anticoagulant sample collection tubes. Interventional Med Appl Sci. 2016;8(4):181–183. doi:10.1556/1646.8.2016.4.4

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